RESUMO
Oligomer 12 S alpha-toxin as well as 3 S alpha-toxoid of Staphylococcus aureus induced the formation of monoclonal antibodies (mabs). Mabs against the 12 S alpha-toxin could be demonstrated in 31 and those against 3 S alpha-toxoid in 18 of 120 hybrid cell colonies. Each of these mab-preparations reacted with 12 S, 3 S alpha-toxin and 3 S alpha-toxoid. The reactions were more pronounced with the homologous than the heterologous toxin preparations. Mabs against 12 S alpha-toxin inhibited the hemolytic effects of native 3 S alpha-toxin as well or better than the respective polyclonal antisera.
Assuntos
Anticorpos Monoclonais , Toxinas Bacterianas/farmacologia , Proteínas Hemolisinas , Hemólise/efeitos dos fármacos , Animais , Complexo Antígeno-Anticorpo , Toxinas Bacterianas/imunologia , Toxinas Bacterianas/isolamento & purificação , Hibridomas/imunologia , Cinética , Camundongos , Staphylococcus aureus/análiseAssuntos
Toxinas Bacterianas/imunologia , Vacinas Bacterianas/imunologia , Proteínas Hemolisinas/imunologia , Coelhos , Infecções Estafilocócicas/veterinária , Staphylococcus aureus/imunologia , Animais , Injeções Intradérmicas/veterinária , Infecções Estafilocócicas/prevenção & controle , Vacinação/veterináriaRESUMO
Concentrations of alpha 2-macroglobulin could be determined in the sera of 215 blood donors and 94 patients with various internal diseases by quantitative inhibition of an acid protease from Staphylococcus aureus, M 135 (fig. 1, 2). The determinations agreed closely with those obtained by immunodiffusion (tab. 1, fig. 3). However, the alpha 2-macroglobulin-measurements by the protease method required only 4 h and 40 microliter serum. This procedure would also be suitable for the determination of alpha 2-macroglobulin in sera from experimental and domestic animals.