Supercritical Carbon Dioxide Extraction of Coumarins from the Aerial Parts of Pterocaulon polystachyum.

Pterocaulon polystachyum is a species of pharmacological interest for providing volatile and non-volatile extracts with antifungal and amebicidal properties. The biological activities of non-volatile extracts may be related to the presence of coumarins, a promising group of secondary metabolites. In the present study, leaves and inflorescences previously used for the extraction of essential oils instead of being disposed of were subjected to extraction with supercritical CO2 after pretreatment with microwaves. An experimental design was followed to seek the best extraction condition with the objective function being the maximum total extract. Pressure and temperature were statistically significant factors, and the optimal extraction condition was 240 bar, 60 °C, and pretreatment at 30 °C. The applied mathematical models showed good adherence to the experimental data. The extracts obtained by supercritical CO2 were analyzed and the presence of coumarins was confirmed. The extract investigated for cytotoxicity against bladder tumor cells (T24) exhibited significant reduction in cell viability at concentrations between 6 and 12 µg/mL. The introduction of green technology, supercritical extraction, in the exploration of P. polystachyum as a source of coumarins represents a paradigm shift with regard to previous studies carried out with this species, which used organic solvents. Furthermore, the concept of circular bioeconomy was applied, i.e., the raw material used was the residue of a steam-distillation process. Therefore, the approach used here is in line with the sustainable exploitation of native plants to obtain extracts rich in coumarins with cytotoxic potential against cancer cells.

Topical Nanoemulsions as Delivery Systems for Green Extracts of Pterocaulon balansae Aiming at the Treatment of Sporotrichosis.

Coumarins are benzopyrones found in several plant genera, including Pterocaulon (Asteraceae). These compounds represent an important source of new treatments, especially as antimicrobial and antifungal agents. In this study, two coumarin-rich extracts from Pterocaulon balansae using green technologies were obtained through aqueous maceration (AE) and supercritical fluid extraction (SFE). Such extracts were incorporated into nanoemulsions (NAE and NSFE) composed of a medium-chain triglyceride oil core stabilized by phospholipids. The nanoemulsions exhibited droplet sizes between 127 and 162 nm, pH above 5.0, and viscosity of approximately 1.0 cP, properties compatible with the topical route. The coumarins permeation/retention from formulations through ear porcine skin using Franz-type diffusion cells were evaluated. Whatever the extract, coumarins were distributed in skin layers, especially in the dermis in both intact and impaired (tape stripping) skin. In addition, a significant increase in coumarins that reached up to the receptor fluid was observed for impaired skin, with increases of approximately threefold for NAE and fourfold for NSFE. Finally, antifungal activity of nanoemulsions was evaluated according to minimum inhibitory concentrations, and the values were 250 µg/mL for all strains tested. The overall results demonstrated the feasibility of incorporating P. balansae extracts into nanoemulsions and showed a potential alternative for the treatment of sporotrichosis.

Effects of grape juice intake on the cell migration properties in overweight women: Modulation mechanisms of cell migration in vitro by delphinidin-3-O-glucoside.

Overweight and obesity are typical conditions of chronic low-intensity systemic inflammatory responses, and both have become more common in recent decades, which emphasizes the necessity for healthier diet intake. Fruits such as grapes are rich in anthocyanins, one of which is delphinidin, a promising chemopreventive agent with anti-inflammatory properties. Considering that polymorphonuclear cells (PMNs) are rapidly mobilized to tissues when the inflammatory process is initiated, this study aimed to understand the impact of grape juice intake and delphinidin on the migration properties of PMNs. Overweight women ingested 500 mL of grape juice for 28 days, and then lipid and inflammatory profiles, as well as the white blood cell count (WBC), were evaluated. Additionally, the gene expression of inflammatory markers and quantified migration molecules such as CD11/CD18, ICAM-1 and VCAM-1 were evaluated in PMNs. The influence of delphinidin-3-O-glucoside in vitro on some migration properties was also evaluated. Grape juice intake did not influence the lipid profile or affect the WBC. However, NFκB gene expression was reduced in PMNs, also reducing the circulating values of IL-8, sICAM-1, and sVCAM-1. The in vitro results demonstrated that delphinidin significantly reduced the migration potential of cells and reduced CD11-/CD18-positive cells, the gene expression of ICAM-1, and the phosphorylation and gene expression of NFκB. Additionally, delphinidin also reduced the production of IL-6, IL-8, and CCL2. Grape juice, after 28 days of intervention, influenced some properties related to cell migration, and delphinidin in vitro can modify the cell migration properties.

Virtual facebow techniques: A scoping review.

STATEMENT OF PROBLEM: Although advances in technology continue to improve the acquisition of patient data and the manufacturing of different oral rehabilitations, the method of transferring clinical information to a virtual environment has not yet been consolidated in the literature. PURPOSE: The purpose of this scoping review was to map the existing literature on different techniques of transferring information from virtual facebows for oral rehabilitation. MATERIAL AND METHODS: This scoping review was structured using a 5-step methodology based on guidelines proposed by Arksey and O'Malley: (1) characterization of the research question, (2) identification of relevant studies, (3) selection of studies, (4) mapping of results, and (5) selection, summary and reporting of the data. The Joanna Briggs Manual for Evidence Synthesis was followed and the review was guided by the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). The guiding question for the development of this review was, "What virtual facebow techniques are being used to transfer anatomic data to the virtual environment?" RESULTS: A total of 1745 articles were found during the search, and 20 were included in this review. Nineteen of the included articles had positive results with the described techniques of registration and transfer of anatomic references to the virtual environment; however, 1 study indicated that the technique was negative. CONCLUSIONS: Based on the findings, facial scanning, 2-dimensional photographs, and cone beam computed tomography are feasible methods of acquiring extraoral anatomic landmarks. The use of a device that allows the convergence of intraoral and extraoral images by superimposing data was revealed to be a promising option.

Muscle activity between dentate and edentulous patients rehabilitated with dental prostheses: A systematic review.

BACKGROUND: Adequate muscle activity is important for the success of oral rehabilitation: it maintains the integrity of the stomatognathic system and is responsible for chewing movements needed to break, crush and grind food. OBJECTIVE: To compare muscle activity using electromyography (EMG) while clenching or chewing soft and/or hard foods among individuals with natural dentition (ND) and edentulous patients rehabilitated with dental prostheses. METHODS: This review was conducted until March, 2023, and the research question was "Is the muscle activity of edentulous patients rehabilitated with dental prostheses similar to that of dentate individuals?" A search strategy was conducted in PubMed/MEDLINE, The Cochrane Library, Web of Science, Lilacs, Embase and manual journal searches. RESULTS: Ten studies were included in the analysis. Most of them reported that individuals with ND had significant higher muscle activity (clenching or chewing) than complete dentures (CD) users. However, no difference was observed between patients with full mouth implant-supported fixed dental prosthesis (ISFDP) and ND. Additionally, two studies compared patients with mandibular ISFDP with maxillary CD and individuals with ND and found no differences; however, one study concluded that patients with ISFDP with CD (maxillary or mandibular) had lower muscle activity than individuals with ND. Only one study reported a higher muscle activity in patients with full-mouth ISFDP than in individuals with ND. CONCLUSIONS: Bimaxillary CD users had lower muscle activity than individuals with ND. During rehabilitation, the muscle activity of patients with full-mouth ISFDP and mandibular ISFDP with maxillary CD is similar to individuals with ND.

Delphinidin-3-O-glucoside in vitro suppresses NF-κB and changes the secretome of mesenchymal stem cells affecting macrophage activation.

OBJECTIVE: Anthocyanins are polyphenols that are promising chemopreventive agents. They stand out for their anti-inflammatory properties, with specific modulatory actions on the immune system. Additionally, regarding the immune system, a group of cells identified as mesenchymal stem cells (MSCs) have been attracting attention, mainly because of their capacity to migrate to sites of inflammation and produce potent immunomodulatory effects. Considering the ability of these cells to act on the immune system, as well as the properties of anthocyanins, especially delphinidin, in modulating the immune system, the aim of this study was to investigate the effects of delphinidin in influencing some immunoregulatory properties of MSCs. METHODS: MSCs were cultivated in the presence of delphinidin 3-O-ß-d-glycoside and cell viability, the cell cycle and the production of soluble factors (interleukin [IL]-1ß, IL-6, IL-10, transforming growth factor [TGF]-ß, prostaglandin E2 [PGE2] and nitric oxide [NO]) were evaluated, as was the expression of the transcription factors nuclear factor (NF)-κB and STAT3. Additionally, the effects of conditioned media from MSCs on macrophage activation were assessed. RESULTS: Delphinidin at 50 µM does not affect cell viability. In association with lipopolysaccharide, delphinidin was able to induce MSC proliferation. Additionally, delphinidin modulated the MSC immune response, showing increased levels of anti-inflammatory cytokines such as IL-10 and TGF-ß as well as lower expression of NF-κB. Furthermore, conditioned media from MSCs inhibited macrophage metabolism, reducing the production of IL-1ß, IL-12, and TNF-α and increasing IL-10. CONCLUSIONS: Overall, this work showed that delphinidin can modify the immunomodulatory properties of MSCs, increasing the IL-10 production by macrophages.

Itinerários terapêuticos produzidos por mulheres na busca pelo acesso ao diagnóstico e cuidado no câncer de mama

Objetivo: mapear o acesso ao diagnóstico e os impactos no cuidado em mulheres com câncer de mama em um serviço de referência estadual no nordeste do Brasil. Métodos: Abordagem qualitativa exploratória, com dados coletados por meio de entrevista a partir de um roteiro semiestruturado. O local escolhido para a coleta dos dados foi um Centro Estadual de Oncologia. A amostra foi composta por 11 participantes, embasando-se na saturação das respostas para a sua composição. A interpretação de dados foi realizada pelo método de análise de conteúdo. Resultados: As entrevistas revelam que a produção do diagnóstico em câncer de mama se inicia por diversos caminhos, um deles é por meio dos mutirões de mamografia, ou seja, através de campanhas focalizadas. Nesse percurso, as mulheres acessam diferentes pontos da rede de atenção, enfrentando as lacunas deixadas pelos serviços de saúde, como o déficit de comunicação e a fragmentação do cuidado. Portanto, é necessário que haja uma interlocução efetiva entre os serviços, visando minimizar os atrasos no diagnóstico e no início da terapêutica, possibilitando, assim, a continuidade da atenção. Conclusões: O diagnóstico do câncer por muitas vezes ainda é realizado por campanhas de detecção, mas, para tanto, há ainda itinerários produzidos por estas mulheres com muitas barreiras que dificultam o acesso aos resultados. Uma dessas barreiras é a comunicação, a qual se estabelece um obstáculo importante para continuidade do cuidado e consequentemente a resolutividade precoce desta condição de saúde.

Influência da Delfinidina como potencial modulador da capacidade imunorregulatória das Células-Tronco Mesenquimais / Influence of Delphinidin as a potential modulator of the immunoregulatory capacity of Mesenchymal Stem Cells

As Células-Tronco Mesenquimais (CTMs), são células multipotentes, presentes em diversos tecidos, sendo bastante estudada devido sua capacidade imunorregulatória por meio da liberação de fatores solúveis. Fatores estes que atuam sobre as funções de células do sistema imunitário. Simultaneamente, estudos indicam que os compostos flavonoides, em destaque a Delfinidina, presente em alguns frutos e flores, possuem atuação anti-inflamatória e inibitória sobre células do sistema imunitário. Todavia, são escassos os estudos em relação entre a capacidade imunorregulatória da CTM e a influência da Delfinidina, sendo este o objetivo deste estudo. Inicialmente, a Delfinidina 3-O-ß-D-glicosídeo foi escolhido, devido a sua maior estabilidade e a dose de 50 µM foi selecionada após análise por citometria de fluxo que mostrou aumento da fase proliferativa do ciclo celular. Posteriormente ao realizar análise da produção de fatores solúveis pelas CTM, os resultados mostraram aumento da produção de IL-10, TGF-ß e Oxido nítrico pelas CTM tratadas com Delfinidina. Bem como, diminuição da expressão de p-NF-κB/NF-κB pelas CTMs tratadas com Delfinidina, quando avaliadas por Wersten Blot. Adicionalmente, para analisar a Delfinidina sobre os efeitos imunorregulatórios da CTM sob macrófagos (RAW 264.7), célula esta, importante no sistema imune inato. Foram realizadas culturas condicionadas, com posterior análise da produção de fatores solúveis, os resultados mostraram aumento da produção de IL-10, e diminuição da produção de TNF-α, IL-1α e IL-12 pelos macrófagos, nas culturas condicionadas. Assim como, diminuição da expressão do fator p-NF-κB/NF-κB pelos macrófagos nas culturas condicionadas, quando avaliadas por Wersten Blot. Ademais, ao analisar a atividade metabólica dos macrófagos por ensaio de MTT, os resultados mostraram que as culturas condicionadas e a Delfinidina per si foi capaz de diminuir a atividade metabólica, sem alterar os efeitos anti-inflamatórios sobre a célula. Em síntese, a Delfinidina mostrou acentuar a atuação imunorregulatória da CTM sobre a linhagem macrofágica, célula esta, de grande importância para o sistema imune inato

Mesenchymal Stem Cells (MSCs) are multipotent cells present in various tissues, being widely studied due to their immunoregulatory capacity through the release of soluble factors. These factors act on the functions of cells of the immune system. Simultaneously, studies indicate that flavonoid compounds, especially Delphinidin, present in some fruits and flowers, have anti inflammatory and inhibitory effects on immune system cells. However, there are few studies on the relationship between the immunoregulatory capacity of MSC and the influence of Delphinidin, which is the objective of this study. Initially, Delphinidin 3-O-ß-D-glycoside was chosen due to its greater stability and the 50 µM dose was selected after analysis by flow cytometry which showed an increase in the proliferative phase of the cell cycle. Subsequently, when analyzing the production of soluble factors by MSCs, the results showed an increase in the production of IL-10, TGF-ß and nitric oxide by MSCs treated with Delphinidin. As well as decreased expression of p-NF-κB/NF-κB by MSCs treated with Delphinidin, when evaluated by Wersten Blot. Additionally, to analyze Delphinidin on the immunoregulatory effects of MSC on macrophages (RAW 264.7), this cell is important in the innate immune system. Conditioned cultures were performed, with subsequent analysis of the production of soluble factors, the results showed an increase in the production of IL-10, and a decrease in the production of TNF-α, IL-1α and IL-12 by macrophages, in the conditioned cultures. As well as decreased expression of p-NF-κB/NF-κB factor by macrophages in conditioned cultures, when evaluated by Wersten Blot. Furthermore, when analyzing the metabolic activity of macrophages by MTT assay, the results showed that conditioned cultures and Delphinidin itself was able to decrease the metabolic activity, without altering the anti-inflammatory effects on the cell. In summary, Delphinidin has shown to enhance the immunoregulatory action of MSC on the macrophage lineage, a cell that is of great importance for the innate immune system

Bioaccessibility and Cellular Uptake of Carotenoids Extracted from Bactris gasipaes Fruit: Differences between Conventional and Ionic Liquid-Mediated Extraction.

Currently, on an industrial scale, synthetic colorants are used in many fields, as well as those extracted with conventional organic solvents (COSs), leading to several environmental issues. Therefore, we developed a sustainable extraction and purification method mediated by ionic liquids (IL), which is considered an alternative high-performance replacement for COSs. Carotenoids are natural pigments with low bioaccessibility (BCT) and bioavailability (BV) but with huge importance to health. To investigate if the BCT and cellular uptake of the carotenoids are modified by the extraction method, we conducted a comparison assay between both extraction procedures (IL vs. COS). For this, we used the Amazonian fruit Bactris gasipaes, a rich source of pro-vitamin A carotenoids, to obtain the extract, which was emulsified and subjected to an in vitro digestion model followed by the Caco-2 cell absorption assay. The bioaccessibility of carotenoids using IL was better than those using COS (33.25%, and 26.84%, respectively). The cellular uptake of the carotenoids extracted with IL was 1.4-fold higher than those extracted using COS. Thus, IL may be a feasible alternative as extraction solvent in the food industry, replacing COS, since, in this study, no IL was present in the final extract.

Dispositivos intraorais no tratamento da apneia obstrutiva do sono em pacientes desdentados: uma revisão sistematizada de literatura / Intraoral devices in the treatment of obstructive sleep apnea in edentulous patients: a systematized literature review

Objetivo: elucidar aspectos envolvidos no uso dos dispositivos intraorais para apneia obstrutiva do sono em indivíduos desdentados totais, como a eficácia dos dispositivos, o conforto e retenção/estabilidade. Metodologia: essa revisão seguiu o checklist do PRISMA, no qual foram incluídos estudos clínicos em inglês, sem restrição de tempo, em que foram utilizados dispositivos para apneia obstrutiva do sono em pacientes desdentados bimaxilares. As buscas foram realizadas nas bases de dados PubMed / MEDLINE, Cochrane e SCOPUS até março de 2021. Resultados: Após as diferentes etapas do processo de seleção dos artigos, foram selecionados 6 estudos para esta revisão, sendo 5 relatos de caso e 1 ensaio clínico. Os estudos relataram uma redução no índice apneia-hipopneia. Em três estudos houve redução expressiva, proporcionando a redução no grau de apneia, de severa para moderada e moderada para leve. A protrusão alcançada com os dispositivos foi adequada para o efeito desejado, em todos os dispositivos. Os estudos não reportam deslocamento do dispositivo e apenas um relata desconforto temporário. Conclusão: Os dispositivos intraorais foram eficazes no tratamento da apneia obstrutiva do sono em pacientes desdentados e os usuários não tiveram queixas quanto ao conforto e estáveis... (AU)

Objective: to elucidate aspects involved in the use of intraoral devices for obstructive sleep apnea in complete edentulous patients, such as the effectiveness of these devices, comfort and retention/stability. Methodology: this review followed the PRISMA checklist, included clinical studies in English, without publication restrictions, in which intraoral devices were used for obstructive sleep apnea in edentulous bimaxillary patients. The search was carried out in PubMed/MEDLINE, Cochrane and SCOPUS databases, until March 2021. Results: After the different stages of the article selection process, 6 studies were selected for this review, 5 case reports and 1 clinical trial. The studies reported a reduction in the apnea-hypopnea index. In three studies there was a significant reduction in the degree of apnea, from severe to moderate and moderate to mild. The protrusion achieved with the devices was adequate for the desired effect, in all devices. Studies did not report displacement of the device and only one reports temporary discomfort. Conclusion: Intraoral devices were effective in treating obstructive sleep apnea in edentulous patients and users had no complaints about comfort and stability... (AU)

Existe relação entre doenças respiratórias e próteses dentárias removíveis contaminadas? Uma revisão sistematizada da literatura / Relationship between respiratory diseases and contaminated removable dental prostheses

Objetivo: O objetivo dessa revisão sistematizada da literatura foi analisar a associação entre o uso de próteses dentárias removíveis e doenças respiratórias prevalentes. Materiais e métodos: Este estudo foi conduzido seguindo os critérios do PRISMA check-list (Preferred Reporting Items for Systematic Review and Meta-Analysis). A base de dados eletrônica selecionada foi a PubMed/MEDLINE, sem restrições do ano de publicação. Estudos prospectivos e retrospectivos (ensaios clínicos randomizados, ensaios clínicos controlados, estudos de coorte, estudos caso-controle e estudos transversais), estudos in vitro e publicados em inglês foram escolhidos como critérios de elegibilidade. Resultados: A busca inicial na base de dados obteve 553 artigos e 8 deles foram selecionados baseados nos critérios de elegibilidade e subdivididos em dois tópicos: doença pulmonar obstrutiva crônica e pneumonia por aspiração. Conclusão: Com base nos estudos avaliados existe associação entre as próteses dentárias removíveis contaminadas e doenças respiratórias... (AU)

Objective: The purpose of this review was to analyze the association between the use of removable dental prostheses and the prevalence of respiratory diseases. Methodology: This study was conducted following the criteria of PRISMA check-list (Preferred Reporting Items for Systematic Review and Meta-Analysis). The database selected was PubMed/MEDLINE, with no restrictions on the year of publication. Prospective and retrospective studies (randomized clinical trials, controlled clinical trials, cohort studies, case-control studies and cross-sectional studies), in vitro studies and published in English were selected in tehe eligibility criteria. Results: The search in the database obtained 553 articles and 8 of them were selected based on the eligibility criteria and subdivided into two topics: chronic obstructive pulmonary disease and aspiration pneumonia. Conclusion: Based on the studies evaluated, there is an association between contaminated removable dental prostheses and respiratory diseases... (AU)

Optimization of Coumarins Extraction from Pterocaulon balansae by Box-Behnken Design and Anti-Trichomonas vaginalis Activity.

Trichomonas vaginalis causes trichomoniasis, a nonviral sexually transmitted infection with a high prevalence worldwide. Oral metronidazole is the drug of choice for the treatment of this disease, although high levels of T. vaginalis resistance to this agent are well documented in the literature. This study describes the anti-T. vaginalis activity of an optimized coumarin-rich extract from Pterocaulon balansae. Optimization was performed to maximize extraction of total coumarins by means of a 3-level Box-Behnken design, evaluating the effect of three factors: extraction time, plant : solvent ratio, and ethanol concentration. Optimum conditions were found to be 5 h extraction time and a plant : solvent ratio of 1% (w/v) and 60% (v/v) ethanol, which resulted in approximately 30 mg of total coumarins/g of dry plant. The coumarin-enriched extract exhibited a minimum inhibitory concentration of 30 µg/mL and an IC50 of 3.2 µg/mL against T. vaginalis, a low cytotoxicity, and a high selectivity index (18 for vaginal epithelial cells and 16 for erythrocytes). The coumarins permeation/retention profile through porcine vaginal mucosa was evaluated in Franz-type diffusion cells. After 8 h of kinetics, coumarins were detected in the tissue (4.93 µg/g) without detecting them in the receptor compartment. A significant increase of coumarins in the mucosa layers (8.18 µg/g) and receptor compartment (0.26 µg/g) was detected when a T. vaginalis suspension (2 × 105 trophozoites/mL) was previously added onto the mucosa. No alterations were visualized in the stratified squamous non-keratinized epithelium of the porcine vaginal mucosa after contact with the extract. Overall, these results suggest that the P. balansae coumarin-rich extract may have potential as a treatment for trichomoniasis.

Influence of Complete Denture Use on Respiratory Capacity: A Systematic Review.

AIM AND OBJECTIVE: This study aimed to determine whether the use of complete dentures has an influence on the respiratory capacity, assessed by the spirometry examination. MATERIALS AND METHODS: A systematic review was conducted following the Preferred Reporting Items for Systematic Review and Meta-Analysis and registered in the International Prospective Register of Systematic Reviews (CRD42021255224). The PICO question (population/exposure/comparison/outcome) was "Does the use of complete dentures influence the respiratory capacity of a toothless subject?" A search strategy was adapted for the PubMed/MEDLINE, The Cochrane Library, OpenGrey, Lilacs, Scopus, and Embase databases. Inclusion criteria were prospective and retrospective studies. The new castle ottawa (NOS) scale and the Methodological Index for Non-randomized studies were selected to assess the quality of the included studies. RESULTS: Four studies were selected, totalizing the evaluation of 242 participants, aged ranging from 40 to 73 years old. Two studies concluded that the use of complete dentures can negatively affect the respiratory capacity. One study stated that it did not interfere regardless of its use during spirometric measurements, and the other reported that dental prosthesis was required in cases of evaluation of the extrathoracic airways. CONCLUSION: The use of complete dentures did not represent relevant changes from the reference values for pulmonary function in the spirometry test. Considering the results of this review, it is not yet possible to establish a clinical protocol for the use of complete dentures during the test. CLINICAL SIGNIFICANCE: Oral rehabilitation with conventional complete dentures is widely used for the treatment of edentulism, especially in elderly patients. In addition, with aging, many complex changes in immunity and respiratory function contribute to the increase in the development of lung diseases. Therefore, it is important to establish a guidance regarding the use or not of the removable dental prostheses in the respiratory capacity test through spirometry examination.

Bioaccessibility and cellular uptake by Caco-2 cells of carotenoids and chlorophylls from orange peels: A comparison between conventional and ionic liquid mediated extractions.

Native extracts from orange peels were obtained by a conventional method using acetone and, an alternative method using ionic liquid (1-butyl-3-methylimidazolium chloride ([C4mim]Cl)). The bioaccessibilities and cellular uptakes of carotenoids, esters and chlorophylls were evaluated, since the influence of esterification on bioaccessibility and bioavailability is not well established. For this, the extracts were emulsified, submitted to in vitro simulated digestion model according to the INFOGEST protocol, followed by uptake by Caco-2 cells. Compounds were separated, identified and quantified by HPLC-PDA-MS/MS. After digestion, 22.0% and 26.2% of the total carotenoids and 45.9% and 68.7% of the chlorophylls were bioaccessible from the acetone and [C4mim]Cl extracts, respectively. The bioaccessibilities of xanthophylls and carotenes were significantly higher than those of the mono- and diesters. The uptake by Caco-2 cells varied from 130.2 to 131.9 ng/mg cell protein for total carotenoids and from 243.8 to 234.2 ng/mg cell protein for chlorophylls in the acetone and [C4mim]Cl extracts, respectively. In general, xanthophylls and esters were better absorbed than carotenes.

Perfil sociodemográfico e odontológico de usuários de próteses totais do programa de extensão universitária Resgatando Sorrisos / Sociodemographic and dental profile of users of total protheses in the university extention program "Resgatando Sorrisos"

Objetivo: Realizar uma análise transversal do perfil sociodemográfico e clínico de pacientes edêntulos bimaxilares e dos hábitos de uso das suas próteses totais (PT) antigas. Posteriomente, os participantes foram reabilitados com próteses totais, em um período de 3 anos (2017- 2019)), em três municípios do estado de Pernambuco - Brasil, por meio de um programa de extensão universitária. Métodos: 58 participantes foram avaliados por meio de um questionário composto de 73 questões. Os dados coletados permitiram uma avaliação quantitativa, e o nível de significância considerado foi de 0,05. Resultados: Destaca-se um perfil de pacientes idosos com uma média de tempo de desdentado total de 24,3±15,3 anos (maxila) e 22,14±14,22 (mandíbula), havendo uma maior prevalência de usuários com próteses bimaxilares (74%). Destaca-se em 57,1% da amostra, a predo-minância da escovação com creme dental e, em 42,9%, a utilização de sabão/detergente, solução de hipoclorito de sódio (NaClO) e/ou bicarbonato de sódio (NaHCO3) como coadjuvantes na higienização. Além disso, 66,6% da amostra apresentou necessidade de intervenção clínica para reparos na PT. Com relação aos hábitos deletérios, os idosos apre-sentaram menos o hábito de dormir com a prótese quando comparados a adultos (p≤0,001). Conclusão: O perfil dos participantes apresentou prevalência do gênero feminino e idade média de 63 anos, além de uma média de 20 anos desdentados (maxila e mandíbula), um tempo elevado de uso das próteses (>10 anos) e o hábito de uso contínuo. Mais da metade dos participantes utilizava métodos de higienização inadequados.

Objective: To conduct a cross-sectional analysis of the sociodemographic and clin-ical profile of edentulous bimaxillary patients and their habits of using old complete den-tures (CD).Subsequently, the participants were rehabilitated with complete dentures, in three years (2017-2019), in three municipalities located in the state of Pernambuco, - Bra-zil, through a university extension program. Methods: We evaluated a total of 58 partici-pants through a questionnaire with 73 questions. Data collected were used for quantitative assessment, and the level of significance was set at 0.05. Results: Most of the participants were elderly patients with an average total edentulous time of 24.3 ± 15.3 years, and 22.14 ± 14.22 years at the maxilla and mandible, respectively. In addition, there was a high prev-alence of users who had bimaxillary prostheses (74%). In 57.1% of the sample, there was a predominance of brushing with toothpaste, 42.9% used soap/detergent, sodium hypochlo-rite solution, and (or) sodium bicarbonate as adjuvants in cleaning. Moreover, 66.6% of the patients needed a clinical intervention for CD repairs. About the harmful habits, the elderly showed a less frequent habit of sleeping with their prostheses compared to the adults (p ≤ 0.001). Conclusion: Most of the participants were female with an average age of 63 years. They presented an average of 20 years of edentulousness (maxilla and mandible) and the extended use of the dentures (> 10 years), in addition to the habit of continuous use. More than half of the participants used inadequate cleaning methods.

Atividades esquistossomicida da planta victoria amazonica / Schistosomicidal activities of the victoria amazonica plant

A esquistossomose é uma doença parasitária que afeta 240 milhões de pessoas ao redor do mundo e mais de 700 milhões vivem em áreas endêmicas, sendo um problema de saúde pública. Atualmente, o praziquantel (PZQ) é o principal fármaco disponível para esta doença, porém está em uso clínico há mais de 30 anos com relatos de resistência. Neste contexto, a busca por novas terapias é de extrema importância. Metabólitos secundários presentes em plantas são extremamente utilizados como protótipos farmacêuticos. Considerando que o Brasil apresenta uma das maiores biodiversidades do mundo, muitos candidatos a fármacos antiparasitários vêm sendo estudados. A planta Victoria amazonica pertence a família Nymphaeaceae, é amplamente encontrada na região amazônica. Apesar de nenhum estudo etnofarmacológico ter sido publicado até o momento, pouquíssimos trabalhos descrevem alguns constituintes químicos da planta. No presente trabalho, o potencial esquistossomicida da V. amazonica foi avaliado em modelo in vitro utilizando vermes adultos de Schistosoma mansoni. O extrato total metanólico da planta foi particionado em diferentes solventes, obtendo-se uma fração em hexano (VAFH), acetato de etila (VAFA) e diclorometano (VAFD). Após incubação das frações com o parasito a 300 µg/mL por até 96 horas, observou-se a morte dos vermes após 48 h em todas as frações. Estudos em microscopia óptica demonstraram que as frações induziram descamações e bolhas no tegumento dos vermes (macho e fêmea), porém, apenas a fração VAFA induziu a formação de ovos com morfologia alterada. Houve redução na motilidade nas primeiras 24 horas e ausência a partir de 48 horas nas três frações analisadas. Estes dados demonstram que metabólitos presentes em frações orgânicas da planta V. amazonica apresentam atividade antiparasitária, e que futuros isolamento(s) do(s) composto(s) ativo(s) poderão proporcionar a identificação de novos protótipos de fármacos para a esquistossomose.

Representação social do consumo de álcool em idosos de uma população quilombola / Social representation of alcohol consumption among elders of a quilombola population / La representación social del alcohol en una población de edad avanzada quilombo

OBJETIVO: compreender as palavras evocadas por idosos de uma população quilombola acerca do consumo de álcool. MÉTODO: trata-se de uma pesquisa de abordagem mista (quanti-qualitativa). Utilizou-se a Técnica de Evocação Livre de Palavras com o termo indutor "Consumo de bebidas alcoólicas". A análise foi realizada através do software EVOC 2005. Utilizou-se um formulário com questões sociodemográficas, com análise no software SPSS, versão 21. RESULTADOS: foram entrevistados 60 idosos, a maioria do sexo feminino e com baixa escolaridade. CONCLUSÃO: as representações sociais acerca do consumo de bebidas alcoólicas se mostraram negativas, o que emerge significações atreladas ao alto consumo de álcool e à problematização advinda com este.

OBJECTIVE: understand the words evoked by elders of a quilombola population about alcohol consumption. METHOD: this is a mixed-approach (quantitative and qualitative) research. The Free-Speech Evoking Technique was used with the inductive term "alcohol consumption". The analysis was performed using EVOC 2005 software. A form with sociodemographic questions was used, and the statistical analysis was performed with SPSS software, version 21. RESULTS: participants interviewed were 60 elderly, mostly were female and with low schooling. CONCLUSION: the social representations on the alcohol consumption were negative, which shows meanings linked to the high consumption of alcohol and to the problems deriving from this consumption.

OBJECTIVO: comprender las palabras evocadas por ancianos de una población quilombola acerca del consumo de alcohol. METODO: se trata de una investigación de enfoque mixto (cuantitativa). Se utilizó la Técnica de Evocación Libre de Palabras con el término inductor "Consumo de bebidas alcohólicas" el análisis fue realizado a través del software EVOC 2005. Se utilizó un formulario con cuestiones sociodemográficas, con análisis en el software SPSS versión 21. RESULTADOS: fueron entrevistados 60 ancianos, mayoría del sexo femenino y con baja escolaridad. CONCLUSIÓN: las representaciones sociales, acerca del consumo de bebidas alcohólicas se mostraron negativas, lo que emerge significaciones atadas al alto consumo de alcohol y la problematización que viene con este.

Recent Patents Concerning the use of Nanotechnology-based Delivery Systems as Skin Penetration Enhancers.

Nanotechnology-based delivery systems have been considered a promising approach for topical application, considering their characteristics of penetration into/across the skin. The present review aimed to evaluate the recent international scenario of patents concerning the use of nanotechnology- based delivery systems as skin penetration enhancers. A survey of recent patent documents was conducted by using the Espacenet patent database including the terms "skin" in the title and "promot* or enhanc* and penetrat* or absorp* or permeat*" and "nano*" with the truncation symbol (*) in the abstract of documents. A total of 110 patents were published from 2008 to 2018, with 94 technologies being considered. The results demonstrated an increase in innovations concerning nanotechnologybased delivery systems as skin penetration enhancers in recent years. Most patent applicants are from China (60.6%) and Korea (21.3%), and companies (68%) were the most prominent owners. The majority of patent applications (76%) were intended for cosmetic purposes; the types of products and nanostructures were also investigated. Overall results demonstrated the increased interest around the world in patenting products involving skin permeation promotion and nanotechnology for pharmaceutical and, mainly, for cosmetics purposes.

Brazilian Biodiversity Fruits: Discovering Bioactive Compounds from Underexplored Sources.

Large segments of the Brazilian population still suffer from malnutrition and diet-related illnesses. In contrast, many native fruits have biodiversity and are underexploited sources of bioactive compounds and unknown to consumers. The phytochemical composition of nine underexplored Brazilian fruits was determined. Carotenoids and anthocyanins were identified and quantified by high performance liquid chromatography-photodiode array-tandem mass spectrometry (HPLC-PDA-MS/MS), and phenolic compounds and iridoids were identified by flow injection analysis-electrospray-ion trap-tandem mass spectrometry (FIA-ESI-IT-MS/MS); in total, 84 compounds were identified. In addition, the chemical structure and pathway mass fragmentation of new iridoids from jenipapo ( Genipa americana) and jatoba ( Hymenae coubaril) are proposed. The highest level of carotenoids was registered in pequi ( Caryocar brasiliense; 10156.21 µg/100 g edible fraction), while the major total phenolic content was found in cambuci ( Campomanesia coubaril; 221.70 mg GAE/100 g). Anthocyanins were quantified in jabuticaba ( Plinia cauliflora; 45.5 mg/100 g) and pitanga ( Eugenia uniflora; 81.0 mg/100 g). Our study illustrates the chemical biodiversity of underexplored fruits from Brazil, supporting the identification of new compounds and encouraging the study of more food matrixes not yet investigated.

Impacto da autopercepção de saúde em longevos com incontinência urinária / Impact of self-perceived health on long-lived subjects with urinary incontinence

OBJETIVOS: Verificar o impacto da autopercepção de saúde sobre a chance de desenvolver incontinência urinária em longevos. MÉTODOS: Trata-se de um estudo transversal e analítico com idosos de 90 anos ou mais. Foi realizada análise estatística analítica e descritiva (frequências, média e desvio padrão, análise de regressão logística univariada e ajustada pela autopercepção de saúde) das variáveis sociodemográficas e clínicas (sexo, estado conjugal, continência, idade, sintomas depressivos, cognição, comorbidades e facilidade de realizar atividades básicas e funcionais). RESULTADOS: Participaram 182 longevos, 71% mulheres. O diagnóstico prévio de depressão, o escore de sintomas depressivos e o percentual de facilidade para atividades funcionais e básicas foram relacionados com a autopercepção de saúde (p<0,05). A perda de urina, contudo, não foi relacionada à autopercepção de saúde. A autopercepção de saúde influenciou o efeito das variáveis diagnóstico prévio de depressão, número de sintomas depressivos, pontuação do Mini Exame do Estado Mental e número de comorbidades sobre a chance de incontinência urinária na presença das mesmas. O sexo masculino, a viuvez e a facilidade no desempenho de atividades básicas e funcionais foram variáveis significativamente relacionadas com a incontinência urinária independentemente do ajuste da autopercepção de saúde. CONCLUSÕES: Não houve relação entre incontinência urinária e autopercepção de saúde, contudo, a autopercepção de saúde influenciou no efeito das variáveis sociodemográficas e clínicas sobre a chance de ter incontinência urinária.

AIMS: To verify the impact of self-perceived health on the chance of developing urinary incontinence in very-old. METHODS: This is a cross-sectional and analytical study with elderly individuals aged 90 years and over. The statistical and analytical variables (sex, marital status, continence, age, depressive symptoms, cognition, comorbidities and ability to perform activities) were analyzed analytical and descriptive statistics (frequencies, mean and standard deviation, univariate and health self-perception adjusted logistic regression analysis). RESULTS: There were 182 participants, 71% women. The previous diagnosis of depression, the score of depressive symptoms and the percentage of ease for functional and basic activities were related to self-perception of health (p<0.05). The loss of urine, however, was not related to self-perceived health. The self-perception of health influenced the effect of the variables previous diagnosis of depression, number of depressive symptoms, Mini-Mental State Examination score and number of comorbidities on the chance of urinary incontinence in the presence of these variables. Males, widowhood and ease of performance of basic and functional activities were significantly related to urinary incontinence regardless of the adjustment of health self-perception. CONCLUSIONS: There was no relationship between urinary incontinence and health self-perception. However, health self-perception influenced the effect of sociodemographic and clinical variables on the chance of having urinary incontinence.