Application of metal artifact reduction algorithm for CBCT diagnosis of temporary anchorage device-tooth root contact: inadequate to reduce false-positive rate.

OBJECTIVES: It was recently found that when cone beam computed tomography (CBCT) was used to examine temporary anchorage device (TAD)-tooth root contact, it tends to yield high false-positive (FP) diagnoses. This study investigated whether application of a metal artifact reduction (MAR) algorithm or reducing CBCT scan voxel-size can remediate this problem. METHODS: 18 fresh pig cadaver mandibles underwent TAD placement bilaterally at first molar lingual furcation regions. CBCT scans were taken under varied MAR (absence, presence) and voxel-size (400 µm, 200 µm) settings. Then, TADs were removed and a micro-CT scan (27 µm voxel-size) of the TAD placement site was performed. Three raters, blinded of CBCT scan setting, independently diagnosed whether TADs were in contact with roots. The reliability and accuracy of CBCT diagnoses using micro-CT as the gold-standard were statistically examined. RESULTS: Generally, CBCT diagnoses had intrarater (Cohen's κ: 0.54-1) and interrater (Fleiss' κ: 0.73-0.81) reliability, within the moderate to excellent range, which did not vary with MAR setting or scan voxel-size. For diagnostic accuracy, FP rate among all raters was mostly in the 15-25% range and did not change with MAR or scan voxel-size settings (McNemar tests, p > 0.05) while false-negative rate was relatively minimal and only occurred to one rater (9%). CONCLUSIONS: When using CBCT to diagnose possible TAD-root contact, applying a currently available Planmeca MAR algorithm or reducing CBCT scan voxel-size from 400 µm to 200 µm may not decrease FP rate. Further optimization of the MAR algorithm for this purpose may be needed.

Multiple Defects of Natural Killer Cells in Cancer Patients: Anarchy, Dysregulated Systemic Immunity, and Immunosuppression in Metastatic Cancer.

We have previously demonstrated that natural killer (NK) cells are the main immune effectors that can mediate selection and differentiation of different cancer stem cells and undifferentiated tumors via lysis and secreted or membrane-bound interferon-γ and tumor necrosis factor-α, respectively. This leads to growth inhibition and tumor metastasis curtailment. In this review, we present an overview of our findings on NK cell biology and its significance in selection and differentiation of stem-like tumors using in vitro and in vivo studies conducted in nonobese diabetic/severe combined immunodeficiency (scid)/interleukin-Rγ--, humanized-bone-marrow/liver/thymus (hu-BLT) mice, and those of human cancer patients. Moreover, we present recent advances in NK cell expansion and therapeutic delivery and discuss the superiority of allogeneic supercharged NK cells over their autologous counterparts for cancer treatment. We review potential loss of NK cell numbers and function at neoplastic and preneoplastic stages of tumorigenesis as a potential mechanism for pancreatic cancer induction and progression. We believe that NK cells should be placed highly in the armamentarium of tumor immunotherapy due to their indispensable role in targeting cancer stem-like/poorly differentiated tumors and a variety of other key NK cell functions that are discussed in this report, including their role in CD8+ T-cell expansion and targeting gene knockout or dedifferentiated tumors. The combination of allogeneic supercharged NK cells and other immunotherapeutic strategies such as oncolytic viruses, antibody-dependent cellular cytotoxicity-inducing antibodies, checkpoint inhibitors, chimeric antigen receptor (CAR)-T cells and CAR-NK cells, chemotherapeutics, and radiotherapeutic strategies can be used for optimal eradication of tumors.

Use of evidence-based assessments for childhood anxiety disorders within a regional medical system.

Anxiety disorders represent a common and serious threat to mental health in children and adolescents. To effectively treat anxiety in children, clinicians must conduct accurate assessment of patients' symptoms. However, despite the importance of assessment in the treatment of childhood anxiety disorders, the literature lacks a thorough analysis of the practices used by clinicians' when evaluating such disorders in community settings. Thus, the current study examines the quality of assessment for childhood anxiety disorders in a large regional health system. The results suggest that clinicians often provide non-specific diagnoses, infrequently document symptoms according to diagnostic criteria, and rarely administer rating scales and structured diagnostic interviews. Relatedly, diagnostic agreement across practice settings was low. Finally, the quality of assessment differed according to the setting in which the assessment was conducted and the complexity of the patient's symptomatology. These results highlight the need to develop and disseminate clinically feasible evidence-based assessment practices that can be implemented within resource-constrained service settings. (PsycINFO Database Record