RESUMO
Cervical swabs for Chlamydia culture were collected from 638 unselected women attending a sexually transmitted diseases clinic with a fresh complaint. Chlamydia were isolated from 76 (12 per cent.) of the women. When the results were related to the patients' diagnoses, Chlamydia were present in 44 per cent. of women with gonorrhoea and in 22 per cent. of women who were contacts of men with nonspecific urethritis (women who may be regarded as having non-specific genital infection). Chlamydia were uncommon in women with no evidence of genital infection. Significant correlations were found between the presence of Chlamydia and cervical erosion, cervical cytological inflammatory change, and absence of symptoms. Isolates were obtained more frequently from women with non-specific genital infection who were primary contacts than from women who were secondary contacts. These findings support the concept that Chlamydia are pathogens in the genital tract and are sexually transmitted.
Assuntos
Colo do Útero/microbiologia , Chlamydia/isolamento & purificação , Infecções Sexualmente Transmissíveis/microbiologia , Uretra/microbiologia , Infecções por Chlamydia/transmissão , Anticoncepcionais Orais , Feminino , Humanos , Masculino , Infecções Sexualmente Transmissíveis/transmissão , Uretrite/microbiologia , Erosão do Colo do Útero/microbiologiaRESUMO
The effect of minocycline on nongonococcal urethritis (NGU) was investigated, with particular reference to its action against Chlamydia trachomatis. Preliminary laboratory studies showed that the drug was active against a laboratory chlamydial strain (Lb4f) in vitro. A group of 133 men with first attacks of NGU was then treated with minocycline, 100 mg twice daily for 3 weeks. Isolates of C trachomatis were obtained from 33 of these men before treatment; all of them gave negative results on cell culture after therapy. Of the 133 patients, 119 (90%) had symptoms on their first attendance, but after one week's treatment only 16 of the 133 (12%) and after 3 weeks 6 of the 133 (5%) had symptoms. All 133 men had demonstrable urethritis initially; by the end of the first week of treatment only 43 of the 133 (32%) and by the end of the third week 32 of the 133 (24%) had evidence of urethritis. There were no differences in clinical response between the Chlamydia-positive and Chlamydia-negative groups. A group of 24 female sexual contacts of men with NGU yielded C trachomatis on cell culture. After 3 weeks' therapy with minocycline all these women gave negative results on cell culture. It is concluded that minocycline is a useful addition to existing remedies for NGU. The future structure of treatment trials for NGU is discussed.
Assuntos
Infecções por Chlamydia/tratamento farmacológico , Chlamydia trachomatis , Minociclina/uso terapêutico , Tetraciclinas/uso terapêutico , Uretrite/tratamento farmacológico , Animais , Embrião de Galinha , Chlamydia/efeitos dos fármacos , Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis/efeitos dos fármacos , Chlamydia trachomatis/isolamento & purificação , Avaliação de Medicamentos , Feminino , Humanos , Masculino , Minociclina/efeitos adversos , Minociclina/farmacologia , Tetraciclina/farmacologia , Uretrite/microbiologia , Cervicite Uterina/tratamento farmacológico , Cervicite Uterina/microbiologiaRESUMO
Men with urethritis due to Neisseria gonorrhoeae were treated with gentamicin, which is inactive against Chlamydia. Urethral specimens were collected before treatment and one and two weeks after treatment and cultured for Chlamydia in irradiated McCoy cells. The overall incidence of chlamydial infection was 15 of 44 (34%). All of 15 Chlamydia-positive men and 11 of 29 Chlamydia-negative men (38%) developed postogonococcal urethritis two weeks after treatment. Pre-treatment sera were examined by a complement fixation test and a simplified indirect fluorescent antibody (FA) test with a yolk sac-cultured antigen, strain SA2)f). Sera from all of 15 Chlamydia-positive men had titers of larger than or equal to 1:16 in the indirect FA test; 13 of 29 Chlamydia-negative men (45%) had positive tests. The complement fixation test was insensitive, detecting chlamydial antibodies in only one of 15 sera from Chlamydia-positive men. These results suggest that Chlamydia may cause many cases of postgonococcal urethritis.
Assuntos
Infecções por Chlamydia/imunologia , Gonorreia/complicações , Uretrite/imunologia , Anticorpos/análise , Chlamydia/imunologia , Chlamydia/isolamento & purificação , Infecções por Chlamydia/tratamento farmacológico , Testes de Fixação de Complemento , Técnicas de Cultura , Imunofluorescência , Gentamicinas/uso terapêutico , Humanos , Masculino , Neisseria gonorrhoeae/imunologia , Uretrite/etiologia , Uretrite/microbiologiaRESUMO
In the diagnosis of trichomoniasis Papanicolaou-stained cervical smears gave marginally better results than cultures of vaginal secretion; stained cervical smears and wet films of vaginal material gave similar results. Culture of vaginal secretion on Sabouraud's medium gave the best results in the diagnosis of candidiasis; Papanicolaou-stained smears gave significantly fewer positive results than either cultures or Gram-stained vaginal smears. Papanicolaou-stained smears were reported as positive in only two of five patients with cervical herpes simplex virus infection. It is concluded that Papanicolaou smears are as good as wet films or cultures in the diagnosis of trichomoniasis but cannot be relied on for the diagnosis of candidiasis, or for detecting herpes simplex virus infection.