Exploratory saccades show no direction-specific deficit in neglect.

In patients with spatial neglect, contralesional reflexive saccades toward suddenly appearing targets show direction-specific deficits. We examined whether these deficits also occur during free exploration of space. Neglect patients' voluntary eye movements showed reduced amplitudes for saccades in all directions but no direction-specific deficit. The results argue against an interpretation of spatial neglect as a general deficit to disengage attention or to program saccades in contralesional direction.

Disentangling gravitational, environmental, and egocentric reference frames in spatial neglect.

Previous studies in neglect patients using rotation of the body around the roll-axis revealed neglect of visual stimuli not only in the egocentric, body-centered left but also in the environmental left. The latter has been taken as evidence for a gravity-based environment-centered component of neglect occurring independently of the subject's actual body orientation. However, by using visual stimuli in a normally lightened room, the studies confounded the gravitational upright with the visible upright of the surround. Thus, it is possible that the visible upright of the environment may have served the role of the gravitational upright relative to which neglect occurred. The present experiment evaluated the influence of gravity on contralateral neglect when no visual information was presented. In complete darkness, neglect patients' exploratory eye movements were recorded in five experimental conditions: body in normal upright position, body titled 30 degrees to the left and 30 degrees to the right, and body pitched 30 degrees backward and 30 degrees forward. In the upright orientation, the patients with neglect showed a bias of ocular exploration to the ipsilesional right side. In egocentric body coordinates, we found no significant differences between the orientation of the biased search field in the different experimental conditions showing that the search field shifted with the orientation of the body. No significant decrease or enhancement of neglect was observed when body orientation was varied in the different conditions. In conclusion, the present results revealed that the modulation of gravitational forces has no significant influence on the exploratory bias of these patients. When visual information was excluded and only graviceptive information was available, the patients' failure to explore the contralesional part of space appeared purely body-centered. The results argue against a disturbed representation of space in neglect that encodes locations in a gravity-based reference system.

Changes of cerebrovascular CO2 reactivity during normal aging.

BACKGROUND AND PURPOSE: During the past decade, transcranial Doppler sonography has widely been used to assess blood flow velocities in the basal intracranial arteries and cerebrovascular reactivity (CR) to various stimuli. Although numerous studies have shown a decline of cerebral blood flow velocity with age, the age dependency of CR, including cerebrovascular CO2 reactivity, however, is controversial. Recently, we have reported a significant sex-related difference in CR, stressing the need to study the relation between normal aging and CR in both sexes separately. METHODS: By means of transcranial Doppler sonography, CR was determined in 100 healthy, nonsmoking volunteers (age 20 to 70 years, 10 men and 10 women per decade). RESULTS: In men, no change of CR with increasing age could be observed (P=0.98). In contrast, CR in women declined significantly, with a step decrease from the 4th to the 5th decades (F=4.413; P<0.01) and was significantly higher in the 3rd and 4th compared with the 5th, 6th, and 7th decades (P<0.05). Information on hormone replacement therapy (HRT) in women of the 6th and 7th decades was obtained retrospectively. HRT was associated with enhanced CR (HRT, n = 7 versus non-HRT, n = 13; P<0.001), with values similar to those found in premenopausal women. CONCLUSIONS: There are no changes of CR during normal aging in men, whereas CR declines significantly from the 4th to the 5th decades in women. HRT in postmenopausal women appears to enhance CR.

Space exploration in neglect.

The present study investigated the gaze as well as the head and the eye-in-head movements of neglect patients while they were exploring their surroundings. A random configuration of letters was presented on the inner surface of a sphere that surrounded the subject, requiring free exploratory eye and head movements. The subjects were requested to search for a single (non-existent) target letter. The co-ordination of eye and head movements in patients with neglect resembled the pattern usually observed in healthy subjects orienting to eccentric visual targets. They performed hypometric head movements with additional shifts of eye-in-head position. Moreover, like healthy subjects, the patients with neglect explored space with gaze, with head and eye-in-head movements that were symmetrically distributed around preferred orientations in space. However, in contrast to controls, these centres of exploration were shifted towards the right. The average horizontal position of gaze and of head movements lay right of the body's mid-sagittal plane, the average eye-in-head position right of the head midline. The preferred orientations were located far away from the anatomical limits of horizontal gaze, head and eye-in-head movements. The decrease of exploration towards more eccentric locations left and right of these orientations thus could not be explained by anatomical restrictions. The results argue against a model of neglect that proposes a lateral gradient of attentional orienting towards the ipsilesional side. Exploring the surroundings, the patients did not orient gaze, the head or the eyes in the head towards the extreme ipsilesional side, nor even close to it. The results favour a deviation model suggesting a shift of the whole frame for exploratory behaviour towards the ipsilesional side. In addition to this shift, we found a second component of altered visual exploration in neglect. The patients' head and gaze movements exhibited a reduced variability around the deviated centre of exploration. The variability was not generally reduced but rather concerned specifically the horizontal dimension. The latter was found even when the area of exploration was paralleled between the groups, requiring the control subjects to search only in that part of the letter array that the neglect patients had explored spontaneously. Possible mechanisms, such as a disturbed ability to update the spatial representation of visual targets or an altered neural representation of space in the horizontal dimension, are discussed.

Increased bronchoalveolar IgG2/IgG1 ratio is a marker for human lung allograft rejection.

BACKGROUND: Lung allograft rejection (AR) is thought to involve T-helper-1 (Th-1) lymphocytes mediating both cellular immunity and alloantibody production. Th-1 lymphocytes produce gamma interferon (gamma IFN) and induce IgG2 production, suggesting that increased IgG2 production might occur during AR. The purpose of this study was to determine if locally altered bronchoalveolar IgG2/IgG1 ratios might correlate with AR. METHODS: Eighteen recipients of lung allografts underwent a total of 25 bronchoscopies for surveillance or at times of suspected infection or AR. Bronchoalveolar lavage (BAL), serum collection, and transbronchial biopsy (TB) were performed on all patients. gamma IFN, IgG1, IgG2 levels, and the ratio of IgG2/IgG1 were determined in serum and BAL and matched with TB histology. Five nonsmoking normal volunteers undergoing bronchoscopy, BAL, and serum collection served as controls. RESULTS: IgG2 was upregulated in allograft BAL during AR as determined by the ratio IgG2/IgG1 (2.91 +/- 0.79 SEM vs 0.62 +/- SEM, p < 0.019, IgG2/IgG1, AR BAL vs non-AR BAL, respectively). An IgG2/IgG1 > or = 1 in allograft BAL (95% confidence intervals 1.26 to 4.56) was 80% specific and 91% sensitive for the diagnosis of AR with a positive predictive value of 92%. A BAL IgG2/IgG1 < 1 (95% confidence interval 0.27 to 0.97) had a negative predictive value of 77%. After therapy in two patients the elevated IgG2/IgG1 ratio reversed to normal (ie, < 1) with histologic resolution of AR. CONCLUSIONS: Human lung AR is associated with a locally increased IgG2/IgG1 ratio suggesting locally upregulated Th-1 lymphocyte activity during lung AR.

Severe hypoxia related to uncomplicated atrial septal defect.

A 73-year-old man was admitted to our institution with severe hypoxia. Cardiac catheterization and transesophageal echocardiography revealed an atrial septal defect with an interatrial right-to-left shunt but with no pulmonary hypertension. Direct examination at surgery revealed an elongated thoracic aorta that caused the aortic annulus to reside at the level of the diaphragm and the heart to be positioned transversely in the mediastinum. Surgical closure of the atrial septal defect normalized oxygenation. We attribute the unusual occurrence of an atrial septal defect and a right-to-left shunt in the absence of pulmonary hypertension to the spatial and mechanical changes caused by the patient's elongated aorta and by the transverse position of the heart.