Quantitative method for the determination of antibiotic-resistant gut bacterial strains for the early diagnosis of chronic arthritis.

Based on the clinical and microbiological monitoring of two groups of children aged 3 to 17 years with acute (n=78) and chronic (n=46) course of reactive arthritis (ReA), a method for early diagnosis of chronic arthritis was developed by determining the number of antibiotic-resistant coprostrains in patients with ReA, characterized by the absence of the need to isolate a pure culture of the pathogen and its identification; inoculation of faeces at a dilution of 10-5 on solid 1.5% GRM-agar with an antibacterial agents used in the treatment of a particular patient, at a minimum inhibitory concentration in the resistance range, followed by incubation and counting of the colonies of microorganisms grown on the plate. A significant relationship between the number of antibiotic-resistant gut bacterial strains and the course of arthritis (acute, chronic) was revealed, and the borderline value of the number of antibiotic-resistant gut bacterial strains was determined - 5×103 CFU/g, which allows differentiating the acute course from the chronic one: in the acute course< 5×103 CFU/g, with chronic - ≥ 5×103 CFU/g. The method allows, at the stage of completion of anti-inflammatory therapy in the active phase of the disease, to identify a risk group for the development of a chronic course of arthritis among patients with ReA, which can contribute to timely therapeutic measures aimed at preventing recurrence of the disease and making the patient disabled.

Diagnostic cytokine marker of male infertility - interleukin 4.

Analysis of the study is to assess the diagnostic significance of cytokines in the sperm plasma of men of reproductive age (20 - 45 years) of two groups: of patients with chronic bacterial prostatitis, not complicated by infertility and with loss of fertility. The study of sperm plasma - the WHO standard. Determination of the level of cytokines in seminal plasma - by enzyme immunoassay («Cytokine¼, Russia). Two methods of mathematical statistics were used: discriminant analysis and classification trees (decision trees).The similarity of interpretations of discriminant analysis and decision tree was noted, where the main role in both cases belongs to the cytokine IL-4. The level of sperm IL-4 in combination with therapeutic monitoring can be used for the medical management of patients with chronic prostatitis in order to prevent the development of infertility and to develop methods for screening diagnostics of fertility disorders in men.

[GUT MICROSYMBIOCENOSIS IN CHILDREN WITH REACTIVE ARTHRITIS].

AIM: To study the state of gut microsymbiocenosis in children with reactive arthritis (RA), with the assessment of biofilm formation (BFF) of microsymbionts and the ability to change cytokine levels (their anticyokine activity) in vitro. MATERIALS AND METHODS: The investigation of gut microsymbiocenosis by means of bacteriological method was conducted in 34 children with RA and 25 relatively healthy 3 - 16 year- old children. Microorganisms were identified with the help of MALDI-TOF mass-spectrometry, anticytokine activity (ACA) of microsymbionts - according to Bukharin O.V et al. (2011), biofilm formation - according to O'Toole G.A., Kolter R. (1998). RESULTS: On the ground of species composition differences of gut microbiota discrimination model was created which allowed to separate the group of children with RA from healthy individuals. Microsymbiocenosis of patients with RA was characterized by increasing number of opportunistic microorganisms (OM) (enterobacteria, clostridia, bacteroides, and Candida), BFF and ACA level. CONCLUSION: The obtained data greatly contribute.to the deciphering of spondylo- arthritis and disclose the role of microbial factor under given pathology. Hypercolonisation of human gut with OM, having pronounced ability to BFF and regulating cytokine level, promotes strengthening of arthritogenic potential and serves as additional marker of arthritis development risk in children.

Formation of Helices in Graphene Nanoribbons under Torsion.

We use objective boundary conditions and self-consistent charge density-functional-based tight-binding to simulate at the atomistic scale the formation of helices in narrow graphene nanoribbons with armchair edges terminated with fluorine and hydrogen. We interpret the microscopic data using an inextensible, unshearable elastic rod model, which considers both bending and torsional strains. When fitted to the atomistic data, the simple rod model uses closed-form solutions for a cubic equation to predict the strain energy and morphology at a given twist angle and the crossover point between pure torsion and a helix. Our modeling and simulation bring key insights into the origin of the helical graphene morphologies stored inside of carbon nanotubes. They can be useful for designing chiral nanoribbons with tailored properties.

Ewald summation on a helix: A route to self-consistent charge density-functional based tight-binding objective molecular dynamics.

We explore the generalization to the helical case of the classical Ewald method, the harbinger of all modern self-consistent treatments of waves in crystals, including ab initio electronic structure methods. Ewald-like formulas that do not rely on a unit cell with translational symmetry prove to be numerically tractable and able to provide the crucial component needed for coupling objective molecular dynamics with the self-consistent charge density-functional based tight-binding treatment of the inter-atomic interactions. The robustness of the method in addressing complex hetero-nuclear nano- and bio-systems is demonstrated with illustrative simulations on a helical boron nitride nanotube, a screw dislocated zinc oxide nanowire, and an ideal DNA molecule.

[On laboratory diagnostics of early alcoholism and comorbid visceropathy].

The article is dedicated to methodical approaches to revealing organopathological consequences of alcohol abuse in adolescents. The author describes laboratory criteria of pre-nosological disturbances in homeostasis which characterize functional changes in the somatic sphere and adaptation. Algorithms of biochemical diagnostics of internal diseases accompanying alcohol abuse are presented.

The use of the FEFF8 code to calculate the XANES and electron density of states of some sulfides.

The FEFF8 was used to calculate x-ray absorption spectra (XANES) and the local partial electron density of states (LDOS) for several sulfides: InPS4 (twice defective chalcopyrite), CdIn2S4 (normal spinel) and Tl3AsS3 (space group R3m). The calculated XANES are compared with experimental spectra. The self-consistent MT-potentials found for clusters of about 35 atoms were used to calculate XANES and LDOS for clusters of 87 atoms in the approach of full multiple scattering. In all cases the agreement of the simulated XANES and experimental ones is quite good. The theoretical LDOS agree well with the experimental x-ray emission K- and L2,3-spectra.

[Errors in reconstructive-rehabilitative operations on the hip joint]. / Oshibki pri rekonstruktivno-vosstanovitel'nykh operatsiiakh na tazobedrennom sustave.

Based upon the analysis of 1015 case records of patients, aged 16-70, with different hip joint pathology types, carried out during 1985-1990, there were revealed mistakes and complications after reconstructive-restorative operations. In the group of patients with dysplastic coxarthrosis they noted rather frequent (12% of cases) formation of "tectum" in supratrochanteric region that served as the cause of joint instability and development of arthrosis in future. Cartilaginous limbus, not removed at grave forms of dysplastic coxarthrosis, served as the cause of the pain syndrome in 63% of cases. Carrying out of "osteotomy-medicate" only in patients with aseptic necrosis of femoral head was ineffective in 38% of cases. Total removal of necrotization foci in thigh head with subsequent osseous plasty is necessary. In the process of mobilization operation in case of hyperplastic, coxarthrosis, removal of the lower edge of cotyloid cavity is the obligatory step. Thus, the important condition of favourable terminations of reconstructive-restorative operations is the correct selection of indications, carrying out of complete surgical intervention, rigorous observation of the patient control rules in near and distant postoperative period.

Subchronic nose-only inhalation study of propylene glycol in Sprague-Dawley rats.

Groups of nineteen Sprague-Dawley rats of each sex were exposed by a nose-only inhalation to 0.0, 0.16, 1.0 or 2.2 mg propylene glycol/litre air, for 6 hr/day, 5 days/wk for 90 days. There were no significant differences in respiratory rates, minute volumes or tidal volumes between any of the groups during aerosol exposure. The uniformity of respiratory parameters between dose groups implied that the delivered doses were proportional to the exposure concentrations. The mean terminal body weights were not significantly different from controls for any group of male animals. The mean body weights of the females exposed to 2.2 mg/litre were significantly less than those of female controls from day 50 onwards. This effect, in female rats, was consistent with a decrease in feed consumption for the high-exposure female rats beginning on study day 43. Statistically significant differences between the treated and control groups in certain haematological parameters, serum enzyme activities, other serum chemistry parameters and organ weights did not show clear dose relationships. There was a significant increase in the number of goblet cells or an increase in the mucin content of the existing goblet cells in the nasal passages of the medium- and high-exposure animals. Exposure to the above concentrations of propylene glycol caused nasal haemorrhage and ocular discharge in a high proportion of animals, possibly as a result of dehydration of the nares and eyes.

[Addiction treatment services in the Russian Federation]. / Narkologicheskaia sluzhba v Rossiiskoi Federatsi.

The purpose of this paper is to address issues pertaining to the substantiation of trends in improving the narcological service with regard to the present restructuring of antialcohol policy. It is necessary to give up the practice of locating narcological facilities in accommodated premises and to develop the network of dispensaries by constructing new types of buildings. It is advisable to drastically expand the basis for post-graduate training of psychiatrists-narcologists by establishing in each economic region chairs of narcology at faculties of post-graduate training of physicians within medical schools, as well as departments of post-graduate training of personnel at scientific research institutes. There is a need to dramatically strengthen the structural subdivisions of narcological service, providing curative and preventive care to drug addicts. Narcological service for teenagers is also in need of improvement, right up to the introduction of posts of psychiatrists-narcologists for teenagers at all narcological institutions. Another task of high priority is the creation of legal mechanism for organising within narcological service, on the cooperative basis, one's own industrial and rural enterprises capable of improving social and labour rehabilitation of patients and, in the and, to ensure the transfer of narcological service to cost-accountable and self-financing practice.

[Effect of ethanol on excitatory and inhibitory processes in the visual cortex of rabbits undergoing learning]. / Vliianie étanaola na vozbuditel'nye i tormoznye protsessy v zritel'noi kore krolikov pri obuchenii.

Subcutaneous ethanol injection to waking rabbits in a dose of 2-6 g/kg causes discoordination of movements, a decline in motor reactions to inhibitory and reinforced light flashes, and appearance of equalizing and paradoxical relations in the number of reactions to these stimuli. Under the influence of ethanol, activating and disinhibitory action of the pain reinforcement on neurones of the visual area temporarily weakens, while the disinhibitory influence of light flashes (CS) is preserved. Judging from the dynamics of phasic reactions to the inhibitory light flashes, the ethanol in a dose of 2-6 g/kg does not influence the inhibitory hyperpolarization processes in the cerebral cortex of rabbits. Tonic inhibition of the cortical unit activity temporarily intensifies after the ethanol injection.

[Clinico-experimental research on acute isoproturon poisoning in sheep]. / Kliniko-eksperimentalno prouchvane na ostroto otraviane s izoproturon pri ovtse.

The toxic action of the carbamide preparation isoproturon-50 was followed up after oral administration at a single dose rate of 3600 mg per kg body mass. Studied were also the clinical picture, body temperature, pulse rate and respiratory excursions, appetite, general behaviour and such biochemical indices as calcium, phosphorus, magnesium, carotene, vitamin A, blood sugar, total protein, aspartate aminotransferase and alanine aminotransferase.

[Histological characteristics of the intraorbital glands of pheasants with tuberculosis]. / Khistologichni osobenosti na intraorbitalnite zhlezi pri fazani, bolni ot tuberkuloza.

For the first time in the country are investigated histologically lacrimal and Harderian glands of pheasants, affected by tuberculosis, raised in pens. By histological analysis were reported the results of the cell reactivity in the affected by the tubercular process tissues and organs. Under the influence of the mycobacteria and their disintegration products the tissues react specifically. In pheasants, affected by tuberculosis are established destructive processes in the intraorbital glands. Mainly in the connective tissue of the glands are observed tubercular knots, which morphologically differ from these in the hen. In the interlobular tissue, in the interacinar and peritubular connective tissue exist strongly expressed infiltration with cells of the lymphoid series, phagocytes and heterophils, as a result of the affected-tissue reactivity, on account of the local and diffusional spreading of the tubercular bacillus in the glands.

[Pathomorphological changes in experimental Erysipelothrix infection in lambs]. / Patomorfologichni promeni pri eksperimentalna chervenkova infektsiia u agneta.

Investigations were carried out with eight lambs treated i/v with a broth culture of Erysipelothrix rhusiopathiae at the rate of 10(8) microbial cells per 1 cm3. Following infection the lambs developed polyarthritis. Studied were the morphologic changes in both dead and slaughtered lambs at various intervals after the infection was performed. It was found that from the 2nd up to the 7th day the lesions were characterized by moderate infiltration (neutrophil granulocytes) of the parenchymal organs as well as by dystrophic- and necrotic foci in the liver and hyperemia, edema, and hemorrhages in the spleen, lung, and brain. After the 7th day were found reticulohistiocytic proliferations in the liver and heart, and intracapillary glomerulonephritis, thromboendocarditis, and polyarthritis.

[Forced method of insulin-coma therapy (methodology, effects)]. / Forsirovannyi sposob insulinokomatoznoi terapii (metodika, osobennosti deistviia).

A study of a new regimen of insulin comatose therapy by intravenous instillation has shown the feasibility of determining the minimal comatose dose of insulin and achieving the comatose state in the first days of insulin therapy. This method makes it possible to utilize the advantages of insulin comatose treatment (a more marked arresting antipsychotic action and the remission persistence). At the same time insulin comatose therapy by intravenous instillation permits the physicians to overcome one of the major drawbacks of this treatment, namely the time lag of the therapeutic response associated with the period of time needed to select the minimal comatose dose which was characteristic of the old method.

[Toxic action and deratization effect of acute rodenticide]. / Toksichno deistvie i deratizatsionen efekt na akutniia rodentitsid.

Tested was the toxic action and the deratization effect of the new acutely acting rodenticide EL614 recently introduced into deratization practice. The experiments were carried out with laboratory rats and pigs as well as with the rats found in a swine breeding complex. Studied were the morphologic and histopathologic changes in the internal organs of rats that had swallowed baits carrying the preparation in various concentration. It was found that LD50 for rats up to the 24th hour after swallowing the poison was 4.13 mg/kg, and it was 1.78 mg/kg at the 48t hour, and 1.32 mg/kg at the 72nd hour. Intoxication and death set in as the result of impaired function of almost all organs, which was explained by the lipidotropic action of the preparation. Particularly severe were the lesions (hemodynamic and degenerative) in the brain. In field trials the baits contained EL614 in concentration of 0.005 per cent, showed a low deratization effect the first day after setting them. In this case the effect could be explained by the stress developing among the rodent population. The same baits in amounts surpassing 300-400 g caused intoxication and death in pigs of 20 kg live weight.