RESUMO
Kidney development is regulated by a coordinated reciprocal induction of metanephric mesenchymal (MM) and ureteric bud (UB) cells. Here, established MM and UB progenitor cell lines were recombined in three-dimensional Matrigel implants in SCID mice. Differentiation potential was examined for changes in phenotype, organization, and the presence of specialized proteins using immunofluorescence and bright-field and electron microscopy. Both cell types, when grown alone, did not develop into specialized structures. When combined, the cells organized into simple organoid structures of polarized epithelia with lumens surrounded by capillary-like structures. Tracker experiments indicated the UB cells formed the tubuloid structures, and the MM cells were the source of the capillary-like cells. The epithelial cells stained positive for pancytokeratin, the junctional complex protein ZO-1, collagen type IV, as well as UB and collecting duct markers, rearranged during transfection (RET), Dolichos biflorus lectin, EndoA cytokeratin, and aquaporin 2. The surrounding cells expressed α-smooth muscle actin, vimentin, platelet endothelial cell adhesion molecule 1 (PECAM), and aquaporin 1, a marker of vasculogenesis. The epithelium exhibited apical vacuoles, microvilli, junctional complexes, and linear basement membranes. Capillary-like structures showed endothelial features with occasional pericytes. UB cell epithelialization was augmented in the presence of MM cell-derived conditioned medium, glial-derived neurotrophic factor (GDNF), hepatocyte growth factor (HGF), or fibronectin. MM cells grown in the presence of UB-derived conditioned medium failed to undergo differentiation. However, UB cell-derived conditioned medium induced MM cell migration. These studies indicate that tubulogenesis and vasculogenesis can be partially recapitulated by recombining individual MM and UB cell lineages, providing a new model system to study organogenesis ex vivo.
Assuntos
Rim/embriologia , Células-Tronco/fisiologia , Ureter/embriologia , Animais , Diferenciação Celular , Linhagem Celular , Movimento Celular/fisiologia , Colágeno/farmacologia , Meios de Cultivo Condicionados/farmacologia , Combinação de Medicamentos , Células Epiteliais/ultraestrutura , Células Alimentadoras/fisiologia , Fibronectinas/metabolismo , Fator Neurotrófico Derivado de Linhagem de Célula Glial/metabolismo , Fator de Crescimento de Hepatócito/metabolismo , Rim/citologia , Rim/crescimento & desenvolvimento , Laminina/farmacologia , Células-Tronco Mesenquimais/citologia , Camundongos , Camundongos SCID , Técnicas de Cultura de Órgãos/métodos , Organogênese/fisiologia , Proteoglicanas/farmacologia , Células-Tronco/citologia , Ureter/citologia , Ureter/crescimento & desenvolvimentoAssuntos
Autorradiografia/métodos , Eletroforese em Gel de Poliacrilamida/métodos , Endorribonucleases/análise , Escherichia coli/enzimologia , Sítios de Ligação , Endorribonucleases/metabolismo , Marcação por Isótopo/métodos , RNA/genética , RNA/metabolismo , Ribonucleases/análise , Ribonucleases/metabolismo , Sensibilidade e EspecificidadeRESUMO
Although glycerol is not taken up via the phosphotransferase system (PTS) in Bacillus subtilis, some mutations that affect the general components of the PTS impair the ability of cells to grow on glycerol. Five revertants of a pts deletion mutant that grow on glycerol were analysed. They were shown to carry mutations in the glycerol kinase gene. These are missense mutations located in parts of the glpK gene that could encode regions important for the activity of glycerol kinase. The results strongly suggest that the main effect of the PTS on glycerol utilization in B. subtilis is mediated via glycerol kinase.