[Protective effects of San Baoxin on injury induced by myocardical ischemic reperfusion and anti-thrombosis in rats].

OBJECTIVE: To investigate the effects of San Baoxin on myocardial injury induced by ischemia-reperfusion (MI/R) and thrombogenesis in rats in vivo and ex vivo. METHOD: The experimental model was established by ligation of left anterior descending coronary artery for 30 min followed by reperfusion for 180 min. The Chandler method was used to produced ex vivo thrombosis and an electrical stimulation of common carotid artery was adopted to form in vivo thrombosis respectively, the effect of antithrombosis induced by San Baoxin was observed. RESULT: San Baoxin significantly decreased the content of malondialdehyde (MDA), obviously elevated the activity of superoxide dismutase (SOD) and increased the amount of NO of the serum simultaneously. The San Baoxin at the dosage of 10 g x kg(-1) could remarkably lengthen the OT ( P < 0.05). All San Baoxin dosages could inhibit the formation of ex vivo thrombosis. CONCLUSION: San Baoxin protects the myocardium from injury of ischemic and reperfusion. The protective effect of San Baoxin may be due to that it can dilate vessels, increase the activity of clearance enzyme of free radical and inhibit lipid peroxidation and the formation of ex vivo and in vivo thrombosis.

Effects of Guiyuanfang and autologous transplantation of bone marrow stem cells on rats with liver fibrosis.

AIM: To investigate the therapeutic effects of Guiyuanfang and bone marrow stem cells (BMSCs) on rats with liver fibrosis. METHODS: Liver fibrosis model was induced by carbon tetrachloride, ethanol, high lipid and assessed biochemically and histologically. Liver function and hydroxyproline contents of liver tissue were determined. Serum hyaluronic acid (HA) level and procollagen III level were performed by radioimmunoassay. The VG staining was used to evaluate the collagen deposit in the liver. Immunohistochemical SABC methods were used to detect transplanted BMSCs and expression of urokinase plasminogen activator (uPA). RESULTS: Serum transaminase level and liver fibrosis in rats were markedly reduced by Guiyuanfang and BMSCs. HA level and procollagen III level were also reduced obviously, compared to model rats (HA: 47.18+/-10.97 ng/mL, 48.96+/-14.79 ng/mL; PCIII: 22.48+/-5.46 ng/mL, 26.90+/-3.35 ng/mL; P<0.05). Hydroxyproline contents of liver tissue in both BMSCs group and Guiyuanfang group were far lower than that of model group (1 227.2+/-43.1 microg/g liver tissue, 1390.8+/-156.3 microg/g liver tissue; P<0.01). After treatment fibrosis scores were also reduced. Both Guiyuanfang and BMSCs could increase the expression of uPA. The transplanted BMSCs could engraft, survive, and proliferate in the liver. CONCLUSION: Guiyuanfang protects against liver fibrosis. Transplanted BMSCs may engraft, survive, and proliferate in the fibrosis livers indefinitely. Guiyuanfang may synergize with BMSCs to improve recovery from liver fibrosis.