RESUMO
Paphiopedilum wenshanense, a species of the family Orchidaceae, is endangered in the world with highly ornamental and biological value, the morphology of P. wenshanense is very similar to its relative species, P. bellatulum and P. concolor. However, there are few studies on the molecular biology and phylogeny of this species currently. Therefore, we report its complete chloroplast (cp) genome sequence at the first time, hoping to provide a foundation for its future phylogenetic analysis. The complete chloroplast genome of P. wenshanense was 161,750 bp in size, which contained a large single-copy (LSC) region of 90,656 bp, a small single-copy (SSC) region of 1886 bp, and two inverted repeat (IR) regions of 34,604 bp each. The total GC content was 35.66%. The genome encodes 38 transfer RNA genes, eight ribosomal RNA genes, and 80 protein-coding genes. The phylogenetic analysis indicated that the genetic relationship between P. wenshanense and P. concolor was very close.
RESUMO
The genetic diversity and differentiation of Lilium formolongi Hort. lines in self-inbred early generations were studied by allozyme electrophoresis. The results showed that mean allele number/locus, percentage of polymorphic loci, average observed heterozygosity and average expected heterozygosity were decreased with the increment of self-inbred generations. The purified loci, purified speed, fixed alleles and increment of genetic differentiation were different among lines because of selection and self-inbred. The mean genetic distances among F4 lines were larger than that of F3 lines, serving as another evidence to prove the differentiation among lines.
