Oxidative stress-mediated activation of FTO exacerbates impairment of the epithelial barrier by up-regulating IKBKB via N6-methyladenosine-dependent mRNA stability in asthmatic mice exposed to PM2.5.

In order to comprehend the underlying mechanisms contributing to the development and exacerbation of asthma resulting from exposure to fine particulate matter (PM2.5), we established an asthmatic model in fat mass and obesity-associated gene knockdown mice subjected to PM2.5 exposure. Histological analyses using hematoxylin-eosin (HE) and Periodic Acid-Schiff (PAS) staining revealed that the down-regulation of the fat mass and obesity-associated gene (Fto) expression significantly ameliorated the pathophysiological alterations observed in asthmatic mice exposed to PM2.5. Furthermore, the down-regulation of Fto gene expression effectively attenuated damage to the airway epithelial barrier. Additionally, employing in vivo and in vitro models, we elucidated that PM2.5 modulated FTO expression by inducing oxidative stress. Asthmatic mice exposed to PM2.5 exhibited elevated Fto expression, which correlated with increased levels of reactive oxygen species. Similarly, when cells were exposed to PM2.5, FTO expression was up-regulated in a ROS-dependent manner. Notably, the administration of N-acetyl cysteine successfully reversed the PM2.5-induced elevation in FTO expression. Concurrently, we performed transcriptome-wide Methylated RNA immunoprecipitation Sequencing (MeRIP-seq) analysis subsequent to PM2.5 exposure. Through the implementation of Gene Set Enrichment Analysis and m6A-IP-qPCR, we successfully identified inhibitor of nuclear factor kappa B kinase subunit beta (IKBKB) as a target gene regulated by FTO. Interestingly, exposure to PM2.5 led to increased expression of IKBKB, while m6A modification on IKBKB mRNA was reduced. Furthermore, our investigation revealed that PM2.5 also regulated IKBKB through oxidative stress. Significantly, the down-regulation of IKBKB effectively mitigated epithelial barrier damage in cells exposed to PM2.5 by modulating nuclear factor-kappa B (NF-κB) signaling. Importantly, we discovered that decreased m6A modification on IKBKB mRNA facilitated by FTO enhanced its stability, consequently resulting in up-regulation of IKBKB expression. Collectively, our findings propose a novel role for FTO in the regulation of IKBKB through m6A-dependent mRNA stability in the context of PM2.5-induced oxidative stress. Therefore, it is conceivable that the utilization of antioxidants or inhibition of FTO could represent potential therapeutic strategies for the management of asthma exacerbated by PM2.5 exposure.

Solid-state fermentation by S. cerevisiae with high resistance to ferulic acid improves the physicochemical properties of wheat bran and quality of bran-rich Chinese steamed bread.

Wheat bran has numerous health benefits, but its poor processing and sensory properties limit its application in the staple food industry. Fermentation by S. cerevisiae changes the performance of wheat bran. However, high levels of ferulic acid (FA) inhibit S. cerevisiae. The effects of solid-state fermentation of S. cerevisiae with high resistance to FA on the physicochemical properties of wheat bran and the quality of bran-rich Chinese steamed bread (CSB) were investigated. The results showed that the growth of S. cerevisiae was inhibited by FA in a dose-dependent manner. Short-term adaptation strategies efficiently improved the tolerance of S. cerevisiae to FA stress. Compared with the parental strain (PS), fermentation of the short-term adapted strains (adapted strains) significantly increased the FA, total phenol, and soluble dietary fiber content in wheat bran. Wheat bran fermented by the adapted strains had a higher antioxidant capacity than wheat bran fermented by PS. In addition, compared with the PS, the wheat bran fermented by the adapted strains can decrease the hardness, improve the specific volume, and the quality of CSB. Thus, solid-state fermentation of the adapted strain is a potentially effective method to improve the nutritional and physicochemical properties of wheat bran as a cereal food ingredient.

Understanding the multi-scale structure, physicochemical and digestive properties of extruded yam starch with plasma-activated water.

In this study, the synergistic effect of plasma-activated water (PAW) combined with twin-screw extrusion (TSE) on multi-scale structure, physicochemical and digestive properties of yam starch (YS) was studied. PAW-TSE resulted in higher amylose content in YS than TSE alone. Compared with single TSE, the relative crystallinity, short-range ordered degree, and gelatinization enthalpy of YS were increased by PAW-TSE according to the results of X-ray diffraction, Fourier transform infrared, Raman spectroscopy, and differential scanning calorimetry. Furthermore, rapid viscosity and dynamic rheological analysis showed that the peak and breakdown viscosity of PAW-TSE treated YS paste were considerably reduced, and the storage modulus and loss modulus were significantly increased, indicating that the gel strength and thermal stability were improved. In addition, the resistant starch (RS) content of YS treated by PAW-TSE increased from 6.04 % to 21.21 %. Notably, the effect of PAW-TSE on YS enhanced with the preparation time of PAW increased. Finally, correlation analysis indicated that the characteristic indexes of PAW had a significant impact on the long or short-range ordered structure, thermal properties, and in vitro digestibility of YS during extrusion. Therefore, PAW-TSE, as an emerging dual modification technology, will greatly expand the application of extrusion technology.

Effect of plasma-activated water on the formation of endogenous wheat starch-lipid complexes during extrusion.

The aim of this study was to investigate the effect of plasma-activated water (PAW) during extrusion on the formation of endogenous starch complexes with wheat starch (WS) as a model material. Using PAW during the extrusion process resulted in an increase in amylose content from 27.87 % to 30.07 %. Results from Fourier transform infrared spectroscopy, X-ray diffraction, and differential scanning calorimetry indicated that the PAW facilitated the formation of endogenous starch-lipid complexes during extrusion. PAW120 (distilled water treated by plasma for 120 s) showed a better promotion effect than PAW60 (distilled water treated by plasma for 60 s). EWS120 (WS extruded using PAW120) exhibited lower peak viscosity and swelling power, but higher solubility, particle size, and resistant starch content compared with EWS0 (WS extruded using distilled water) and EWS60 (WS extruded using PAW60). In a word, the acidic substances in PAW may lead to hydrolysis of starch and generate more amylose, thus improving the amount of endogenous starch-lipid complexes. The present study provides a novel extrusion method to obtain modified starch with higher RS content than common extrusion, which has potential application in the industrial production of functional foods with low glycemic index.

Treatment of malignant airway stenosis with extracorporeal membrane oxygenation under low-dose anticoagulation: A case report.

The occurrence of airway obstruction due to severe stenosis from lung cancer poses a significant risk of asphyxia. Although the placement of a metallic stent may relieve the obstruction, the procedure is associated with a high risk of asphyxia. To mitigate this risk, extracorporeal membrane pulmonary oxygenation (ECMO) has been proposed to temporarily substitute for cardiopulmonary function during the procedure. However, the use of systemic anticoagulation with heparin during ECMO may increase the likelihood of bleeding during surgery. This case report describes a successful treatment of a patient with malignant central airway obstruction through low-dose heparin veno-venous ECMO. This approach resulted in reduced intraoperative bleeding and invasive operation time, allowing for prompt postoperative withdrawal and recovery.

ACT001 Alleviates chronic kidney injury induced by a high-fat diet in mice through the GPR43/AMPK pathway.

BACKGROUND: Roughly 10 -15% of global populace suffer from Chronic Kidney Disease(CKD). A major secondary disease that can progress to end-stage renal disease (ESRD) is obesity-associated kidney disease (ORG). Although clinical management strategies are currently available, morbidity and mortality rates are increasing. Thus, new solutions are needed. Intestinal permeability, systemic inflammation, and aberrant intestinal metabolites have all been linked to ORG. PURPOSE: ACT001 has anti-inflammatory, redox-regulatory and antitumour activities. The current study was designed to examine how ACT001 affects ORG and analyze the fundamental processes. METHODS: A high-fat diet (HFD) was used to generate ORG in female C57BL/6 J mice. ORG mice were divided into three groups at random: HFD, HFD + ACT001, HFD + polyphosphocholine (PPC). To assess renal and colonic damage, periodic acid-Schiff (PAS) and hematoxylin-eosin (HE) staining were used. Following that, renal inflammation, oxidative stress, lipid deposition, colonic inflammation, and intestinal permeability were evaluated by protein blotting, polymerase chain reaction (PCR), immunohistochemistry, and immunofluorescence staining. Lastly, the SCFAs content was assessed by gas chromatographymass spectrometry. RESULTS: Mice in the HFD group displayed more severe albuminuria, glomerular hypertrophy, renal oxidative damage, inflammation, and lipid accumulation than mice with the normal diet (ND) group, as well as lower levels of intestinal SCFA valproic acid, colonic inflammation, and tight junction protein downregulation. ACT001 treatment restores the content of valproic acid in intestinal SCFAs, promotes the binding of SCFAs to renal GPR43, activates the AMPK signalling pathway. Therefore, it promotes the Nrf2-Keap1 signalling pathway and inhibits the NF-κB signalling pathway. SCFAs, additionally, augment colonic GPR43 concentrations, diminishing NLRP3 inflammasome expression and restoring ZO-1 and occludin protein levels. CONCLUSION: This study is the first to look at ACT001's potential as a treatment for obesity-related kidney disease. Regulating GPR43 and AMPK signalling pathways, By controlling the GPR43 and AMPK signalling pathways, ACT001 improves colitis and the intestinal mucosal barrier, decreases renal lipid deposition, and suppresses inflammation and oxidative stress in the kidneys. According to this study, ACT001 could be a viable ORG therapy option.

pH: A core node of interaction networks among soil organo-mineral fractions.

Mineral-associated organic matter (MAOM) is the largest soil organic carbon (OC) pool with the longest turnover. MAOM is expected to have relatively little sensitivity to climate change due to mineral protection, but its persistence involves several organo-mineral fractions. The uncertainty in the response of specific organo-mineral fractions to climate change hampers the reliability of predictions of MAOM preservation in the future. Here, we applied a sequential chemical fractionation method integrated with network analysis to investigate MAOM stabilization mechanisms across five alpine ecosystems: alpine desert, alpine steppe, alpine meadow, alpine wetland, and alpine forest. Hierarchical cluster analysis revealed grouping of seven extractable OM fractions in MAOM into three OM clusters: a cluster with weak bondings consisting of water-soluble OM (WSOM) and weakly adsorbed fractions (2.1-21.3% of total OC); a cluster with metal-bound complexes comprising Ca-OM complexes and Fe/Al-OM complexes (3.8-12.2% of total OC); and a cluster with strong bonding composed of Al oxyhydroxides, carbonates and Fe oxyhydroxides (12.2-33.5% of total OC). The relative percentages of OM from soils of the five ecosystems in the three clusters exhibited distinct pH dependence patterns. With the increase in pH, the cluster with weak bondings decreased, and that with strong bondings increased, while the one with metal-bound complexes showed a maximum at weakly acidic pH. Organo-mineral fractions and metal cations in MAOM constructed a complex network with pH as the central node. Results suggest that precipitation does not only alter vegetation type and microbial biomass but also regulate soil pH, which is balanced by specific metal cations, thus resulting in particular pH preference of specific OM clusters. These findings demonstrate that soil pH plays a central role in unveiling MAOM dynamics and can serve as a good predictor of soil organo-mineral fractions across alpine ecosystems.

MIR-107/HMGB1/FGF-2 axis responds to excessive mechanical stretch to promote rapid repair of vascular endothelial cells.

The increase of vascular wall tension can lead to endothelial injury during hypertension, but its potential mechanism remains to be studied. Our results of previous study showed that HUVECs could induce changes in HMGB1/RAGE to resist abnormal mechanical environments in pathological mechanical stretching. In this study, we applied two different kinds of mechanical tension to endothelial cells using the in vitro mechanical loading system FlexCell-5000T and focused on exploring the expression of miR-107 related pathways in HUVECs with excessive mechanical tension. The results showed that miR-107 negatively regulated the expression of the HMGB1/RAGE axis under excessive mechanical tension. Excessive mechanical stretching reduced the expression of miR-107 in HUVECs, and increased the expression of the HMGB1/RAGE axis. When miR-107 analog was transfected into HUVECs with lipo3000 reagent, the overexpression of miR-107 slowed down the increase of the HMGB1/RAGE axis caused by excessive mechanical stretching. At the same time, the overexpression of miR-107 inhibited the proliferation and migration of HUVECs to a certain extent. On the contrary, when miR-107 was silent, the proliferation and migration of HUVECs showed an upward trend. In addition, the study also showed that under excessive mechanical tension, miR-107 could regulate the expression of FGF-2 by HMGB1. In conclusion, these findings suggest that pathological mechanical stretching promote resistance to abnormal mechanical stimulation on HUVECs through miR-107/HMGB1/RAGE/FGF-2 pathway, thus promote vascular repair after endothelial injury. The suggest that miR-107 is a potential therapeutic target for hypertension.

iTRAQ-based quantitative proteomics analysis of the effect of ACT001 on non-alcoholic steatohepatitis in mice.

ACT001 is a novel sesquiterpene lactone derivative that has been shown to have significant antitumor and anti-inflammatory effects. However, the effect of ACT001 on nonalcoholic steatohepatitis (NASH) is unknown. Methionine and choline deficient (MCD) diet induced NASH model in C57BL/6J mice. Steatosis, inflammation and fibrosis-related indices of serum and liver tissues were detected by fully automated biochemical analyzer, enzyme-linked immunosorbent assay (ELISA) kit, flow cytometry, hematoxylin and eosin (H&E), Masson and immunohistochemical staining. The results showed that ACT001 reduced serum lipid and inflammatory factor levels, attenuated hepatic steatosis, inflammation and fibrosis, and inhibited hepatic oxidative stress and activation of NOD-like receptor protein 3 (NLRP3) inflammatory vesicles in NASH mice. In addition, 381 differentially expressed proteins (DEPs), including 162 up-regulated and 219 down-regulated proteins, were identified in the MCD group and ACT001 high-dose group using isotope labeling relative and absolute quantification (iTRAQ) technique analysis. Among these DEPs, five proteins associated with NAFLD were selected for real-time fluorescence quantitative PCR (RT-qPCR) validation, and the results were consistent with proteomics. In conclusion, ACT001 has a therapeutic effect on NASH, and the results of proteomic analysis will provide new ideas for the mechanism study of ACT001 for NASH treatment.

Networks of mineral-associated organic matter fractions in forest ecosystems.

Mineral-associated organic matter (MAOM), the largest soil carbon pool, is formed through a series of organo-mineral interaction mechanisms. However, different organo-mineral fractions relevant to specific stabilization mechanisms and their response to environmental variables are poorly understood, which hinders accurate prediction of MAOM preservation under climate change. We applied sequential chemical extraction to separate MAOM into different organo-mineral fractions. To assess of response of different organo-mineral fractions to climate change, alpine forest soils with high environmental sensitivity along a controlled environmental gradient were selected. Residual OM and weakly adsorbed OM were the primary organo-mineral fractions, accounting for approximately 45.1-67.7 % and 16.4-30.6 %, respectively, of the total organic carbon (TOC). Climate exerted considerable indirect effects on the preservation of organo-mineral fractions through weathering and edaphic and biotic variables. Moreover, organo-mineral fractions were closely associated with metal cations (mainly Fe3+/Al3+) and secondary minerals, forming complex networks. Water-soluble OM (WSOM), weakly adsorbed OM and Fe/Al oxyhydroxides-stabilized OM were tightly linked, occupying the central position of the networks, and were closely related to soil pH, moisture and prokaryotic composition, indicating that edaphic and biotic factors might play important roles in maintaining the network structure and topology. In addition, Fe/Al-OM complexes, oxyhydroxides-stabilized OM and residual OM in the network were greatly impacted by climate and weathering factors, including precipitation, temperature and the plagioclase index of alteration (PIA). The complex network among organo-mineral fractions sheds light on MAOM dynamic stabilization for better predicting MAOM preservation under climate change.

High-precision single-pixel 3D calibration method using pseudo-phase matching.

Compressive sensing makes it possible to explore two-dimensional spatial information using a single-point detector. However, the reconstruction of the three-dimensional (3D) morphology using a single-point sensor is largely limited by the calibration. Here we demonstrate a pseudo-single-pixel camera calibration (PSPC) method using pseudo phase matching in stereo, which can perform 3D calibration of low-resolution images with the help of a high-resolution digital micromirror device (DMD) in the system. In this paper, we use a high-resolution CMOS to pre-image the DMD surface and successfully calibrate the spatial position of a single-point detector and the projector with the support of binocular stereo matching. Our system achieved sub-millimeter reconstructions of spheres, steps, and plaster portraits at low compression ratios with a high-speed digital light projector (DLP) and a highly sensitive single-point detector.

Debranching facilitates malate esterification of waxy maize starch and decreases the digestibility.

In this study, the debranching followed by malate esterification was employed to prepare malate debranched waxy maize starch (MA-DBS) with a high degree of substitution (DS) and low digestibility using malate waxy maize starch (MA-WMS) as the control. The optimal esterification conditions were obtained using an orthogonal experiment. Under this condition, the DS of MA-DBS (0.866) was much higher than that of MA-WMS (0.523). A new absorption peak was generated at 1757 cm-1 in the infrared spectra, indicating the occurrence of malate esterification. Compared with MA-WMS, MA-DBS had more particle aggregation, resulting in an increase in the average particle size from scanning electron microscopy and particle size analysis. The X-ray diffraction results showed that the relative crystallinity decreased after malate esterification, in which the crystalline structure of MA-DBS almost disappeared, which was consistent with the decrease of decomposition temperature by thermogravimetric analysis and the disappearance of the endothermic peak by differential scanning calorimeter. In vitro digestibility tests showed an order: WMS > DBS > MA-WMS > MA-DBS. The MA-DBS showed the highest content of resistant starch (RS) of 95.77 % and the lowest estimated glycemic index of 42.27. In a word, pullulanase debranching could produce more short amylose, promoting malate esterification and improving the DS. The presence of more malate groups inhibited the formation of starch crystals, increased particle aggregation, and enhanced resistance to enzymolysis. The present study provides a novel protocol for producing modified starch with higher RS content, which has potential application in functional foods with a low glycemic index.

Regression in hepatic fibrosis in elderly Chinese patients with hepatitis C receiving direct-acting antiviral treatment.

BACKGROUND: Patients infected with Hepatitis C virus (HCV) are recommended to receive treatment with direct-acting antiviral agents (DAAs), which have been certified to obtain a high sustained virological response (SVR). However, little is known about the benefits of successful anti-viral treatment to elderly patients with hepatic fibrosis. In this study, we aimed to assess degree of fibrosis in elderly patients with chronic hepatitis C (CHC) treated with DAAs, and to evaluate the correlations between identified factors associated with these changes. METHODS: This study retrospectively enrolled elderly patients with CHC who received DAAs in Tianjin Second People's Hospital from April 2018 to April 2021. The degree of liver fibrosis was assessed using serum biomarkers and transient elastography (TE) expressed as the liver stiffness (LSM), while the hepatic steatosis was evaluated by controlled attenuated parameter (CAP). Changes in factors related to hepatic fibrosis were examined following treatment with DAAs, and associated prognostic factors were further evaluated. RESULTS: We included 347 CHC patients in our analysis, where 127 of these were elderly patients. For the elderly group, the median LSM was 11.6 (7.9-19.9) kPa, and this value was significantly reduced to 9.7 (6.2-16.6) kPa following DAA treatment. Similarly, GPR, FIB-4 and APRI indices were significantly reduced from 0.445 (0.275-1.022), 3.072 (2.047-5.129) and 0.833 (0.430-1.540) to 0.231 (0.155-0.412), 2.100 (1.540-3.034) and 0.336 (0.235-0.528), respectively. While in younger patients, the median LSM reduced from 8.8 (6.1-16.8) kPa to 7.2 (5.3-12.4) kPa, and the trends of GPR, FIB-4 and APRI were also consistent. The CAP in younger patients increased with statistical significance, but we did not observe any significant change in CAP for the elderly group. Based on multivariate analysis, age, LSM, and CAP before baseline were identified as determinants for LSM improvement in the elderly. CONCLUSION: In this study, we found that elderly CHC patients treated with DAA had significantly lower LSM, GPR, FIB-4, and APRI values. DAA treatment did not significantly change CAP. Furthermore, we observed correlations between three noninvasive serological evaluation markers and LSM. Finally, age, LSM, and CAP were identified as independent predictors of fibrosis regression in elderly patients with CHC.

Effects of Different Nanoparticles on Microbes.

Nanoparticles widely exist in nature and may be formed through inorganic or organic pathways, exhibiting unique physical and chemical properties different from those of bulk materials. However, little is known about the potential consequences of nanomaterials on microbes in natural environments. Herein, we investigated the interactions between microbes and nanoparticles by performing experiments on the inhibition effects of gold, ludox and laponite nanoparticles on Escherichia coli in liquid Luria-Bertani (LB) medium at different nanoparticle concentrations. These nanoparticles were shown to be effective bactericides. Scanning electron microscopy (SEM) images revealed the distinct aggregation of cells and nanoparticles. Transmission electron microscopy (TEM) images showed considerable cell membrane disruption due to nanoparticle accumulation on the cell surfaces, resulting in cell death. We hypothesized that this nanoparticle accumulation on the cell surfaces not only disrupted the cell membranes but also physically blocked the microbes from accessing nutrients. An iron-reducing bacterium, Shewanella putrefaciens, was tested for its ability to reduce the Fe (III) in solid ferrihydrite (HFO) or aqueous ferric citrate in the presence of laponite nanoparticles. It was found that the laponite nanoparticles inhibited the reduction of the Fe (III) in solid ferrihydrite. Moreover, direct contact between the cells and solid Fe (III) coated with the laponite nanoparticles was physically blocked, as confirmed by SEM images and particle size measurements. However, the laponite particles had an insignificant effect on the extent of aqueous Fe (III) bioreduction but slightly enhanced the rate of bioreduction of the Fe (III) in aqueous ferric citrate. The slightly increased rate of bioreduction by laponite nanoparticles may be due to the removal of inhibitory Fe (II) from the cell surface by its sorption onto the laponite nanoparticle surface. This result indicates that the scavenging of toxic heavy metals, such as Fe (II), by nanoparticles may be beneficial for microbes in the environment. On the other hand, microbial cells are also capable of detoxifying nanoparticles by coagulating nanoparticles with extracellular polymeric substances or by changing nanoparticle morphologies. Hence, the interactions between microbes and nanoparticles in natural environments should receive more attention.

Recyclable ferroferric oxide@titanium dioxide@molybdenum disulfide with enhanced enzyme-like activity under visible light for effectively inhibiting the growth of drug-resistant bacteria in sewage.

With the development of social industry and the increase in domestic sewage discharge, pathogenic bacterial contamination in water has become a serious health and environmental problem. It is important to design sewage treatment reagents with effective pathogenic bacterial removal and recyclability. In this work, we developed a nanocomposite, Fe3O4@TiO2@MoS2, with once-for-all effects of photocatalytic, magnetic, and peroxidase-like activities for solving the above-mentioned problems. The loading of MoS2 may cause the band gap of Fe3O4@TiO2 to decrease from 3.11 eV to 2.85 eV, demonstrating increased photocatalytic activity under visible light, based on the synergistic impact of Fe3O4@TiO2 and MoS2. In return, the peroxidase-like activity of Fe3O4@TiO2@MoS2 was significantly higher than that of Fe3O4 and MoS2 alone, resulting in the generation of more hydroxyl radicals (˙OH) for combating the drug-resistant broad-spectrum ß-lactamase-producing Escherichia coli and methicillin-resistant Staphylococcus aureus. The antibacterial mechanism study showed that Fe3O4@TiO2@MoS2 could effectively inhibit bacterial growth by destroying the bacterial biofilm and genome via the peroxidase-like activity as well as photocatalytic activity. In addition, Fe3O4@TiO2@MoS2 has excellent paramagnetic properties, which can achieve magnetic recovery after wastewater treatment. Even after three times of recycling, its antibacterial effect can remain above 98.8%.

Cisplatin causes erectile dysfunction by decreasing endothelial and smooth muscle content and inducing cavernosal nerve senescence in rats.

Introduction: Cisplatin (cis-diamminedichloroplatinum II, CDDP), a drug widely used for cancer worldwide, may affect erectile function, but its side effects have not received enough attention. To investigate the effect of CDDP on erectile function and its possible mechanism. Methods: Sprague-Dawley rats were intraperitoneally administered CDDP (CDDP group) or the same volume of normal saline (control group). Erectile function was evaluated after a one-week washout. Then, histologic changes in the corpus cavernosum and cavernous nerve (CN) were measured. Other Sprague-Dawley rats were used to isolate the major pelvic ganglion and cavernous nerve (MPG/CN). RSC96 cells were then treated with CDDP. SA-ß-gal staining was used to identify senescent cells, and qPCR was used to detect the senescence-associated secretory phenotype (SASP). Finally, the supernatant of RSC96 cells was used to culture MPG/CN. Erectile function was measured after administration of CDDP. The cavernosum levels of α-SMA, CD31, eNOS, and γ-H2AX, the apoptosis rate and the expression of p16, p21 and p53 in CN were also assayed. The senescent phenotype of RSC96 cells treated with CDDP was identified, and neurite growth from the MPG/CN was photographed and measured. Results: The CDDP group had a significantly lower ICP/MAP ratio than the control group. Compared to the control group, the CDDP group exhibited significantly lower α-SMA, CD31 and eNOS levels and significantly higher γ-H2AX and apoptosis rates in corpus cavernosum. In addition, CDDP increased some senescence markers p16, p21 and p53 in CN. In vitro, CDDP induced RSC96 senescence and SASP, and the supernatant of senescent cells slowed neurite outgrowth of MPG/CN. Discussions: CDDP treatment could induce erectile dysfunction, by affecting the content of endothelial and smooth muscle and causing SASP in CN. The results indicate that CDDP treatment should be considered as a risk factor for ED. Clinicians should pay more attention to the erectile function of cancer patients who receive CDDP treatment.

Soil texture influences soil bacterial biomass in the permafrost-affected alpine desert of the Tibetan plateau.

Under warm climate conditions, permafrost thawing results in the substantial release of carbon (C) into the atmosphere and potentially triggers strong positive feedback to global warming. Soil microorganisms play an important role in decomposing organic C in permafrost, thus potentially regulating the ecosystem C balance in permafrost-affected regions. Soil microbial community and biomass are mainly affected by soil organic carbon (SOC) content and soil texture. Most studies have focused on acidic permafrost soil (pH < 7), whereas few examined alkaline permafrost-affected soil (pH > 7). In this study, we analyzed soil microbial communities and biomass in the alpine desert and steppe on the Tibetan plateau, where the soil pH values were approximately 8.7 ± 0.2 and 8.5 ± 0.1, respectively. Our results revealed that microbial biomass was significantly associated with mean grain size (MGS) and SOC content in alkaline permafrost-affected soils (p < 0.05). In particular, bacterial and fungal biomasses were affected by SOC content in the alpine steppe, whereas bacterial and fungal biomasses were mainly affected by MGS and SOC content, respectively, in the alpine desert. Combined with the results of the structural equation model, those findings suggest that SOC content affects soil texture under high pH-value (pH 8-9) and that soil microbial biomass is indirectly affected. Soils in the alpine steppe and desert are dominated by plagioclase, which provides colonization sites for bacterial communities. This study aimed to highlight the importance of soil texture in managing soil microbial biomass and demonstrate the differential impacts of soil texture on fungal and bacterial communities in alkaline permafrost-affected regions.

Synthesis and Characterization of PU/PLCL/CMCS Electrospun Scaffolds for Skin Tissue Engineering.

As tissue regeneration material, electrospun fibers can mimic the microscale and nanoscale structure of the natural extracellular matrix (ECM), which provides a basis for cell growth and achieves organic integration with surrounding tissues. At present, the challenge for researchers is to develop a bionic scaffold for the regeneration of the wound area. In this paper, polyurethane (PU) is a working basis for the subsequent construction of tissue-engineered skin. poly(L-lactide-co-caprolactone) (PLCL)/carboxymethyl chitosan (CMCS) composite fibers were prepared via electrospinning and cross-linked by glutaraldehyde. The effect of CMCS content on the surface morphology, mechanical properties, hydrophilicity, swelling degree, and cytocompatibility were explored, aiming to assess the possibility of composite scaffolds for tissue engineering applications. The results showed that randomly arranged electrospun fibers presented a smooth surface. All scaffolds exhibited sufficient tensile strength (5.30-5.60 MPa), Young's modulus (2.62-4.29 MPa), and swelling degree for wound treatment. The addition of CMCS improved the hydrophilicity and cytocompatibility of the scaffolds.

DDSM: Design-Oriented Dual-Scale Shape-Material Model for Lattice Material Components.

This paper proposes a new CAD model for the design of lattice material components. The CAD model better captures the user's design intent and provides a dual-scale framework to represent the geometry and material distribution. Conventional CAD model formats based on B-Rep generate millions of data files, which also makes design intent and material information missing. In the present work, a new shape-material model for lattice material components is proposed. At the macroscopic scale, a compact face-based non-manifold topological data structure is proposed to express the lattice shape-material information without ambiguity. At the microscopic scale, implicit function is adopted for the representation of lattice material components. Numerical experiments verify that the proposed CAD model provides a powerful support for design intent with minor space costs. Meanwhile, the representation method supports solid modeling queries of geometric and material information on each scale.