The value of preoperative controlling nutritional status score in evaluating short-term and long-term outcomes of patients with colorectal cancer following surgical resection.

Background: This study aimed to explore the value of controlling nutritional status (CONUT) score in assessing short-term and long-term outcomes of colorectal cancer (CRC) patients, and construct CONUT-based nomograms to predict risk of postoperative comorbidities and survival. Methods: We retrospectively enrolled 512 patients from 2012 to 2014. Patients were categorized into low-CONUT and high-CONUT groups. Logistic regression analysis was used to determine characteristics influencing postoperative comorbidities. Kaplan-Meier survival curve and Cox proportional hazards analysis were used to determine characteristics affecting prognosis. The receiver operating characteristic was used to compare ability of the CONUT score with other immune-nutritional indicators to predict prognosis. Results: Logistic regression analysis suggested that high CONUT score was an independent risk factor affecting postoperative comorbidities (odds ratio, 1.792; 95% confidence interval [CI], 1.113-2.886; P = 0.016). Patients with low-CONUT score had longer disease-free survival (DFS) (P < 0.001) and overall survival (OS) (P < 0.001) compared to those with high-CONUT score, especially at the early stage. CONUT score was an independent factor affecting both DFS (hazard ratio [HR], 1.820; 95% CI, 1.204-2.752; P = 0.005) and OS (HR, 1.815; 95% CI, 1.180-2.792; P = 0.007). The area under the curve of CONUT score was higher than for other immune-nutritional indicators. The CONUT-based nomograms had good predictive capability. Conclusions: CONUT score is a strong independent predictor of postoperative comorbidities and long-term outcomes in CRC patients, and might be a better prognostic factor than other immune-nutritional indicators. The CONUT-based nomograms are conducive to the individualized formulation of follow-up strategies and treatment plans.

Observation on frequency and effect of dusting molluscicide in irrigation canals / 中国血吸虫病防治杂志

Objective To study a new method and technical specification for Oncomelania snail control in irrigation canals.Methods Four percent niclosamide ethanolamine salt dustable powder was dusted in a test canal three times continuously,and a control canal was set up at the same time.The molluscicidal frequency and effect of niclosamide ethanolamine salt powder was observed and the results,including the change of living snail frames,average density of living snails and mortality of snails,were analyzed.Results Between the third and fifteenth day after the first dusting in the test canal,the reduction rate of the density of snails was more than 90% and after the second and third dusting,the reduction rate was more than 99%.For the average rates of living snail frames and mortality of snails,there were significant differences between the first dusting and later two dustings,while there was no significant difference between the second dusting and the third dusting.On the thirtieth and ninetieth day after the third dusting,the effect of snail control was still satisfactory.There were significant differences between the test canal and control canal about all the observation indexes.Conclusion The application of 4% niclosamide ethanolamine salt dustable powder is efficient in the snail control in irrigation canals,and the suitable frequency of dusting is 2 or 3 times.

P120ctn overexpression enhances beta-catenin-E-cadherin binding and down regulates expression of survivin and cyclin D1 in BEL-7404 hepatoma cells.

AIM: To understand the role of P120ctn in E-cadherin-mediated cell-cell adhesion and signaling as well as in hepatoma cell biological function. METHODS: We stably overexpressed p120ctn isoform 3A in BEL-7404 human hepatoma cells and studied the effect of p120ctn on beta-catenin and E-cadherin binding as well as p120ctn and beta-catenin subcellular localization using immunoprecipitation, Western blotting and confocal microscopy. We also investigated the inhibitory effect of p120ctn transfection on the expression of apoptotic protein survivin survivin and cell cycle regulator cyclin D1 in the cells. RESULTS: Western blotting indicated that p120ctn expression increased after cells were transfected with p120ctn isoform 3A. The protein was located mainly at membrane under immunofluorescent microscope. Beta-catenin nuclear expression was reduced after overexpression of p120ctn isoform 3A. The p120ctn-E-cadherin binding increased after transfection of p120ctn isoform 3A. Furthermore, overexpression of p120ctn down regulated the expression of apoptotic protein survivin and cell cycle regulator cyclin D1. These effects led to reduction of cell proliferation. CONCLUSION: Our results indicate that p120ctn plays an important role in regulating the formation of E-cadherin and -catenin complex, cell apoptosis, cell cycle and cancer cell biological function.

[The relationship between p120ctn translocation and malignant features of hepatocellular carcinoma].

OBJECTIVE: To investigate the effect of catenin p120 (p120ctn) translocation on the malignant features of hepatocellular carcinoma and its interrelation with beta-catenin in E-cadherin-mediated cell signaling. METHODS: Expression and translocation of p120ctn, tyrosine phosphorylation, and its binding capacity to E-cadherin were detected by DNA transfection, immunoblotting and immunoprecipitation. Cellular localization of p120ctn and beta-catenin was detected by immunofluorescent microscopy. Cell adhesion, cell migration and cell proliferation were also studied. RESULTS: Expression of p120ctn increased after cells transfected with p120ctn isoform 3A, and it was located mainly at cell-cell contact region. Its binding to E-cadherin was enhanced. After EGF stimulation, tyrosine phosphorylation of p120ctn was increased, membrane expression of p120ctn and beta-catenin was decreased while cytosol expression was increased. It was translocated into the nucleus, cell adhesiveness was increased but mobility decreased. With over-expression of p120ctn, beta-catenin was recruited by nucleus export. Cell proliferation was reduced but it was increased after EGF treatment. CONCLUSION: p120tn plays an important role in cell adhesion, migration and proliferation of hepatocellular carcinoma, and its tyrosine phosphorylation might contribute to this mechanism. There might be a competitive relationship between p120ctn and beta-catenin.

[p120(ctn) tyrosine phosphorylation are involved in the biologic behavior changes of hepatocellular carcinoma cells].

OBJECTIVE: In hepatocellular carcinoma cells, the tyrosine phosphorylation of p120(ctn) was stimulated by epidermal growth factor (EGF) to investigate the relationship between the tyrosine phosphorylation of p120(ctn) and the translocation of p120(ctn), also the relationship between the tyrosine phosphorylation of p120(ctn) and the biological behaviour of hepatocellular carcinoma cells. The role of p120(ctn) in the cell adhesion and signaling of hepatocellular carcinoma is to be investigated. METHODS: In BEL-7404 human hepatocellular carcinoma cells, the tyrosine phosphotyrosine of p120(ctn) stimulated by EGF were detected by immunoprecipitation (IP) and Immunoblotting (IB). The cellular distribution and translocation of p120(ctn) and beta-catenin were detected and examined by indirect intracellular immunofluorescence. Cell morphology and cell adhesion potential were also detected using correspondent methods. Antisense nucleotide of p120(ctn) was transfected into BEL-7404 cells. RESULTS: The tyrosine phosphorylation of p120(ctn) was enhanced after EGF treatment than control, especially at 20min after EGF treatment; When BEL-7404 cells were transfected with antiseuse nucleotide of p120(ctn) before EGF treatment, the tyrosine phosphorylation of p120(ctn) stimulated by EGF was obviously lowered. We also observed that the tyrosine phosphorylation of p120(ctn) stimulated by EGF was accompanied by the nuclear translocation of p120(ctn); the similar translocation was also observed in beta-catenin after EGF stimulation. At the meantime, cell adhesion potential was reduced after EGF treatment and cell morphology became thin, elongated and irregular, speudopods increased. CONCLUSIONS: In BEL-7404 cells,the tyrosine phosphorylation of p120(ctn) could be stimulated by EGF, which was accompanied by the nuclear accumulation of p120(ctn). The tyrosine phosphorylation of p120(ctn) stimulated by EGF was also in correlation with the changes of cell adhesion and cell morphology. The results indicated that the tyrosine phosphorylation of p120(ctn) cell correlated with the translocation of p120(ctn) and the biological behavior of cells.

Cost-benefit analysis of project with mode of collecting water from middle layer of water body by culvert pipelinet / 中国血吸虫病防治杂志

Objective To evaluate the cost-benefit of the project with the mode of collecting water from middle layer of water body by the culvert pipelinet.Methods The cost-benefit analysis was carried out by the way of benefit-cost ratio for the project with the mode of collecting water from middle layer of water body by the culvert pipelinet without indirect cost and indirect benefit,and according to the price of 2005.Results The direct costs was 34 thousand yuans in the project with the mode of collecting water from middle layer of water body by the culvert pipelinet.The saved costs of prevention and treatment were 1011 thousand yuans,the net benefit was 977 thousand yuans and the net benefit-cost ratio was 28.7∶1 following the project implemented.Conclusion The investment is small but the benefit is high in the project of collecting water from middle layer of water body by the culvert pipelinet.The technique has the notable worthiness of applications.

Efficiency of prevention of Oncomelania snail dispersal with the mode of leading water from middle level of water body by the culvert pipelinet / 中国血吸虫病防治杂志

Objective To evaluate the efficiency of prevention of Oncomelania snail dispersal with the mode of leading water from middle level of water body by the culvert pipelinet in the electric pumping station. Methods The snails were detected in the floats by riverhead and the irrigation areas, and the snails were hold up by the block in the exit of pump. The snail investigation was carried out in the irrigation system areas and the results were compared between the study area and the control area. Results The snails were discovered in the floats of riverhead of the electric pumping station. However, there were no any snails in the exit of pump after the block snail treatment and no any snails in the irrigation areas, either. There were snails in the control areas Conclusion The mode of leading water from middle level of water body by the culvert pipelinet is very effective for the prevention of Oncomelania snail dispersal.