RESUMO
Essential oil from Cymbopogon nardus was evaluated for activity against Trypanosoma brucei brucei BS221 (IC50 = 0.31 ± 0.03 µg/mL) and cytotoxic effect on normal kidney (Vero) cells (IC50 = >100 µg/mL). The crude essential oil was subjected to various chromatography techniques afforded active sub fractions with antitrypanosomal activity; F4 (IC50 = 0.61 ± 0.06 µg/mL), F6 (IC50= 0.73 ± 0.33 µg/mL), F7 (IC50 = 1.15 ± 0 µg/mL) and F8 (IC50 = 1.11 ± 0.01 µg/mL). These active fractions did not exhibit any toxic effects against Vero cell lines and the chemical profiles investigation indicated presence of α-and γ-eudesmol, elemol, α-cadinol and eugenol by GC/MS analysis.
Assuntos
Antiprotozoários/farmacologia , Cymbopogon/química , Óleos Voláteis/farmacologia , Extratos Vegetais/farmacologia , Trypanosoma brucei brucei/efeitos dos fármacos , Animais , Antiprotozoários/química , Antiprotozoários/isolamento & purificação , Antiprotozoários/toxicidade , Sobrevivência Celular/efeitos dos fármacos , Chlorocebus aethiops , Células Epiteliais/efeitos dos fármacos , Células Epiteliais/fisiologia , Cromatografia Gasosa-Espectrometria de Massas , Concentração Inibidora 50 , Óleos Voláteis/química , Óleos Voláteis/isolamento & purificação , Óleos Voláteis/toxicidade , Testes de Sensibilidade Parasitária , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/toxicidade , Células VeroRESUMO
Essential oil from Cymbopogon nardus was evaluated for activity against Trypanosoma brucei brucei BS221 (IC50 = 0.31 ± 0.03 μg/mL) and cytotoxic effect on normal kidney (Vero) cells (IC50 = >100 μg/mL). The crude essential oil was subjected to various chromatography techniques afforded active sub fractions with antitrypanosomal activity; F4 (IC50 = 0.61 ± 0.06 μg/mL), F6 (IC50= 0.73 ± 0.33 μg/mL), F7 (IC50 = 1.15 ± 0 μg/mL) and F8 (IC50 = 1.11 ± 0.01 μg/mL). These active fractions did not exhibit any toxic effects against Vero cell lines and the chemical profiles investigation indicated presence of α-and γ-eudesmol, elemol, α-cadinol and eugenol by GC/MS analysis.
