Functional Activity of Blood Neutrophils in Patients with Stable Course and Exacerbation of Chronic Obstructive Pulmonary Disease.

We performed a comparative study of the parameters of chemiluminescence of blood neutrophils in patients with different severity of chronic obstructive pulmonary disease in its different periods. The maximum values of induced and spontaneous chemiluminescence were recorded at moderate severity of the disease during exacerbation. Low levels of chemiluminescence indicators were found in severe chronic obstructive pulmonary disease in the stable phase. The values of the induction period of the chemiluminescent response in patients with moderate chronic obstructive pulmonary disease were higher than in the control group. Correlations between the values of induced chemiluminescence of neutrophils and the respiration function parameter FEV1 were established, which may indicate the influence of multidirectional changes in the functional activity of systemic neutrophils on the development and worsening of airway obstruction in chronic obstructive pulmonary disease patients.

Cognitive Impairments in Patients with Bronchial Asthma.

The course of bronchial asthma can be accompanied by cognitive impairments. However, the relationship between cognitive dysfunction and asthma has not been fully revealed, nor has it been fully established what causes cognitive impairments in patients with asthma. There is an opinion that transient hypoxia and persistent systemic inflammation with insufficient control of bronchial asthma can be accompanied by neurotoxicity in relation to the hippocampus and indirectly lead to deterioration of cognitive functions. Comorbid conditions, such as obesity, allergic rhinitis, and depressive states can increase cognitive dysfunction in asthmatics. The review considers the pathophysiology of cognitive dysfunction in patients with bronchial asthma, as well as the impact of comorbid conditions on the cognitive status. This information will allow systematizing the available knowledge about the state of cognitive functions in asthma for timely detection and correction of their impairments and, ultimately, optimization of the management of these patients.

Comparative Study of the Levels of IL-1ß, IL-4, IL-8, TNFα, and IFNγ in Stable Course and Exacerbation of Chronic Obstructive Pulmonary Disease of Varying Severity.

In patients with chronic obstructive pulmonary disease, the levels of cytokines IL-1ß, IL-4, IL-8, TNFα, and IFNγ depended on the degree of bronchial obstruction and severity and period of the disease. The maximum levels of IL-4, IL-8, and TNFα were observed in severe chronic obstructive pulmonary disease during exacerbation. The highest concentration of IL-1ß and IFNγ were recorded during activation of inflammation in patients with moderate bronchial obstruction. The revealed correlations between the tested cytokines and spirometry parameters make it possible to consider the levels of these proteins as quantitative markers of systemic inflammation progression.

Messenger RNA of FCGR3A and FCGR3B Genes as Monitoring Markers of Clear Cell Renal Adenocarcinoma (a Pilot Study).

The aim of the study was to assess the capabilities of mRNA genes encoding CD16a (FCGR3A) and CD16b (FCGR3B) in tumor samples from patients with renal cancer, and characterize the tumor process in relation to clinical and morphological factors. Materials and Methods: We used 125 tumor samples from patients with a histologically confirmed diagnosis of renal cancer T1-4N0-1M0-1. A method described by Chomczynski and Sacchi was used to isolate nucleic acids. The mRNA levels were determined using a reverse transcription polymerase chain reaction and calculated according to ΔΔCt formula, taking into account the reaction efficiency. Results: mRNA of the FCGR3A gene was detected in all tumor tissue samples under study; in contrast, mRNA of the FCGR3B gene was found only in 92.0% (115/125) of cases. In tumors classified as pT1, the mRNA content of the FCGR3A gene was significantly lower than that in tumor samples of pT3 size. There was the significant increase in the mRNA content of both genes with an increase in tumor grade, as well as in the cases with distant metastases. The presence of a tumor thrombus in the inferior vena cava system was accompanied by a significant increase in the mRNA content of the FCGR3A gene. Conclusion: In tumor tissue samples from patients with clear cell renal cancer, the predominant production of the FCGR3A mRNA was observed in comparison with the FCGR3B mRNA. The revealed relationship of an increased amount of the FCGR3A mRNA and, in some cases, the FCGR3B mRNA with a number of clinical and morphological factors enables to consider the mRNA level of the genes as new monitoring biomarkers.

[Construction of norovirus (Caliciviridae: Norovirus) virus-like particles containing VP1 of the Echovirus 30 (Picornaviridae: Enterovirus: Enterovirus B)].

INTRODUCTION: Enterovirus (nonpolio) infection is widespread all over the world, registered as sporadic cases and large-scale outbreaks and can cause severe lesions such as serous meningitis. Epidemiological studies have shown that enterovirus (Picornaviridae; Enterovirus) variant Echovirus 30 (E30) is the most frequently detected variant in patients with enterovirus meningitis in the Russian Federation. However, no vaccines to prevent the disease caused by this pathogen have been developed so far. One of the promising modern trends in terms of creating vaccine preparations is the use of virus-like particles (VLPs), including chimeric ones containing the biological structures of viruses belonging to different species.The aim of this work was to obtain norovirus (Caliciviridae; Norovirus) VLPs displaying enterovirus Echovirus E30 full-length VP1 on the surface. MATERIAL AND METHODS: The nucleotide sequences of VP1 protein of norovirus genotype GII.4 and VP1 E30 of genotype h circulating in Russia were used. The SN-VP1E30 protein was constructed, in which the shell (S) and the hinge regions of the norovirus VP1 are fused into one molecule with the full-length VP1 of the E30 virus. The protein was expressed in E. coli, purified using affinity chromatography, and characterized by polyacrylamide gel electrophoresis (PAGE) and immunoblotting. VLPs were visualized by electron microscopy. RESULTS: The S N-VP1E30 protein expressed in E. coli as insoluble form, so the conditions for SN-VP1E30 solublisation were defined. Sucrose has been shown to significantly increase the efficiency of renaturation. Electrophoretic mobility comparison of denatured and non-denatured SN-VP1E30 demonstrated that most monomers form high molecular weight compounds. Electron microscopy showed that renatured SN-VP1E30 spontaneously forms empty virus-like particles about 50 nm in diameter. CONCLUSION: Chimeric protein SN-VP1E30 self-assemble into VLPs displaying the VP1 protein of E30 variant that is highly prevalent in Russia. Further immunological research is necessary to characterize VLPs potential for development of the vaccine for enteroviral meningitis prevention.

[The medical social problems and methods of diagnostic of cognitive disorders in patients taking "Spice"].

The article presents the results of pilot study implemented to determine cognitive disorders in patients taking "Spice". The purpose of research is to identify targets of psychological correction interventions to prevent cognitive deficiency in patients addicted to synthetic cannabinoids. The pilot sampling consisted of 30 patients taking "Spice". The control group consisted of 30 healthy individuals. Along with social consequences, one of the important focuses of the study was investigation of cognitive sphere of patients. A number of pathopsychologic tests was applied. The study established that patients demonstrated disorders of various functions of memory, attention, mental pace and thinking. In particular, disorders of short-term, long-term and mediate memory and mental tempo were revealed. Also, reduced activity of attention took place. The distortion and decreasing of generalization and poor prehension occurred reliably more often as compared with control group. The established cognitive disorders in patients taking "Spice" can be considered as targets for psychological correction interventions to prevent cognitive deficiency in patients addicted to synthetic cannabinoids.

[The mental reactions of psychiatrists and narcologists against the background of COVID-19 pandemic].

The article considers various mental reactions of psychiatrists and narcologists against the background of the SARS-CoV-2 pandemic, based on a brief scientific analysis of publications and experimental psychological research methods. The purpose of study was to identify impact of the COVID-19 pandemic on mental health of psychiatrists and psychiatrists-narcologists. The results of survey of psychiatrists and narcologists are presented. The study was carried out using such test techniques as life quality scale SF-36 (Health Status Survey), Lazarus questionnaire "Coping Behaviors" adjusted to conditions of Russia and Hamilton scales to assess depression and anxiety (HDRS, HARS). The results.The low level of psychological well-being, moderate degree of preference for all coping strategies were established. The anxiety was determine in 34.8% of respondents and depression in 8.7%. It was established that actually, the impact of the COVID-19 pandemic on medical workers is conditioned by complex of mental reactions (from anosognosia to expressed anxiety and depressive disorders) and is accompanied by increasing general anxiety.

S. aureus and E. coli change the force and work of adhesion between P- and E-selectins of endothelial cells and ligands of neutrophil granulocytes.

Thanks to the modification of the force spectroscopy method, when a neutrophil is fixed on the tip, and an endotheliocyte culture is grown on the substrate, the exact indicators of the adhesion force and adhesion work between cells have been investigated. The high variability of adhesion contacts in different donors associated with different expression profiles of neutrophils. It was found by flow cytometry that the EA.hy926 cell line actively expresses VCAM-1, as well as P- and E-selectin under the Staphylococcus aureus influence after 60 min of co-incubation. At the same time, the integral indicators of the adhesion force and adhesion work in the "neutrophil - endothelial cell" interaction were significantly inhibited by S. aureus in all studied donors. Since the VCAM-1 receptor is not involved in the adhesion bonds between neutrophils and endothelial cells, the suppression of the interaction is associated with the inhibition of P- and E-selectins, but direct receptors removal from the endothelial cells surface of the EA.hy926 cell line does not occur. Escherichia coli causes multidirectional effects in the system of interaction "neutrophil - endothelial cell", depending on the expression profile of the donor's neutrophils. However, the cumulative effect of interaction from all donors shows that in general, under the influence of E. coli, there is an increase in adhesion force and a suppression of adhesion work.

[The analysis of failures of intra-familial relationships in families with children suffering of diabetes mellitus].

The article presents the results of study of in-family (child-parents) relationships in families with children suffering with diagnosed diabetes mellitus type I. The purpose of study is to investigate the disorders of family relationships in families of such kind. The established disorders allow to develop psychotherapeutic measures both for children and their parents. Materials and methods. The study covered 30 families with children of primary school age (7-10 years old) with diabetes mellitus. The families with children with severe concomitant diseases and broken families were excluded of the sampling. The control group consisted of 30 relevant families with healthy children. The in-family relationships were explored using a number of psychological tests applied to children and their mothers. The results. In mothers of the studied families prevailed authoritarian type of upbringing, which was expressed in tendency to hyper-protect and to over-control. The phobia of losing child and the tendency to reject it were also established. The children suffered from high level of anxiety. They had more positive attitude to mother than to father. The conclusion. The established disorders of in-family relationships in families with children with diabetes mellitus can serve as targets for psychotherapeutic activities both with children and their parents.

Magnetic phase transition and lattice dynamic features in ErB2C borocarbide.

A sample of erbium borocarbide ErB2C was synthesized from a stoichiometric mixture of erbium, boron, and pyrographite hydride. Temperature dependent magnetic susceptibility, heat capacity and lattice parameters of borocarbide at 2-300 K were experimentally investigated, the Raman spectrum was determined and analyzed. Sharp anomalies in the heat capacity and magnetic properties of ErB2C nearT= 16.3 K, caused by the transition to the antiferromagnetic state, as well as diffuse anomalies at a higher temperature, caused by the effect of the crystal field (Schottky anomalies) were revealed. It was found that the magnetic phase transition does not cause measurable anomalies in the lattice parameters of borocarbide due to the weak coupling between the Er3+ion subsystem and the B-C layers. Almost zero thermal expansion of erbium borocarbide in the basal plane (alongaandbaxes) (anisotropic invar effect) as well as an unusual character of thec-axis thermal expansion were observed. The first of these features is due to the high bonding energy in the B-C layers and the weak bond between the layers in the borocarbide crystal lattice. It was found that the anomalous expansion along thecaxis is a consequence of the action of the crystal field. The splitting scheme for the ground level of Er3+ions by crystal field was determined from the analysis of the Schottky anomaly of the heat capacity.

The specific features of phononic and magnetic subsystems of type-VII clathrate EuNi2P4.

A type-VII clathrate with a Eu2+ guest embedded into a Ni-P covalent framework, EuNi2P4, was synthesized by a standard two-stage ampoule synthesis and confirmed to crystallize in the orthorhombic space group Fddd with unit cell parameters a = 5.1829(1) Å, b = 9.4765(1) Å, and c = 18.9900(1) Å. A general technique for studying the lattice and magnetic properties of REE containing compounds is proposed. The temperature and field dependences of electrical resistivity ρ(T,H), magnetization M(T,H), magnetic susceptibility χ(T,H), heat capacity Cp(T), and unit cell parameters a(T), b(T), c(T), and volume V(T) were experimentally studied and analyzed at different pressures in the temperature range of 2-300 K. A cascade of anomalies in the studied dependences was identified and attributed to the magnetic phase transformation and peculiar lattice contributions at temperatures below 20 K. As a result of comparison with an isostructural clathrate SrNi2P4, the parameters of the magnetic and lattice contributions were determined. It is characteristic that the phase transition from the paramagnetic to the magnetically ordered state is not reflected in the temperature changes of the lattice parameters due to weak bonds between guest europium atoms and the Ni-P host matrix. We have constructed a tentative H-T phase diagram based on the M(T) and M(H) data, which includes 6 different phases. It is established that the anomalous lattice contribution to the clathrate heat capacity CTLS(T) appears due to the effect of two-level systems (TLS) in the Eu2+ subsystem on the thermodynamic properties of EuNi2P4. The values of TLS parameters as well as the parameters of the magnetic subsystem of the clathrate were determined.

Serum Protein Corona Abolishes Changes in the Expression of Proinflammatory Genes Induced by Quantum Dots in Human Blood Mononuclear Cell.

We studied changes in the transcription of genes encoding cytokines (TNF, IL-6, IL-10, and IL-32), cell activation markers (ICAM1, CD38, Fas, and FCGRIII), ROS production catalyst (NOX2), autophagy (Beclin 1, LC3B, and p62) and apoptosis (BAX, BCL2) regulators in peripheral blood mononuclear cells upon contact with quantum dots CdSe/ZnS-MPA and CdSe/CdSeZnS/ZnS-PTVP. Up-regulation of TNF, ICAM1, Fas, p62 mRNA and down-regulation of the FCGRIII and NOX2 mRNA in response to the presence of quantum dots were revealed. The formation of serum protein corona on the surface of quantum dots abolished this effect.

Crystal lattice disorder and characteristic features of the low-temperature thermal properties of higher borides.

Heat capacity CP(T) and lattice parameters a(T), b(T) and c(T) of LuB44Si3.5 borosilicide are experimentally studied as a function of temperature in the range of 2-300 K. The results are compared with those of pseudo-isostructural LuB50 boride. At the lowest temperatures, it is shown that the CP(T) dependence of borosilicide changes linearly with temperature. This is attributed to the effect of glass-like behaviour of the heat capacity due to the disorder in the sublattice of non-metals. The presence of defects in the B-Si sublattice and the irregular form of the cages in the B-Si matrix, which are occupied by Lu3+ ions, lead to the formation of two-level systems (TLS) in the Lu3+ subsystem. The TLS make a characteristic bell-like low-temperature contribution to the heat capacity of borosilicide. We show that there is a wide temperature range (5-150 K) of negative thermal expansion of borosilicide, which is attributed to the influence of quasi-independent vibrations of Lu3+ ions in the cages of the borosilicide crystal structure.

Thermodynamic properties and lattice dynamics investigation of LuB2C: experiment and ab initio calculations.

A sample of lutetium carboboride LuB2C was synthesized from a mixture of lutetium hydride, boron and carbon by annealing in argon. The temperature dependence of the heat capacity Cp(T) (2-300 K) and lattice parameters a(T), b(T), and c(T) (5-300 K) of the carboboride was experimentally determined. The experimental values of the heat capacity were fitted with the approximation Cp(T) = aT + ΣCD + CE + CTLS(T). Here the first term is the electronic contribution, the second is the sum of the Debye components, the third is the Einstein contribution, and the fourth is the contribution to the heat capacity due to the vibrations of the two-level systems which are formed in the Lu-subsystem due to the asymmetry of the B-C atomic arrangement around the Lu3+-ions and, as a consequence, the possible transition of the lutetium atoms between spatially close, but energetically non-equivalent positions. A strong anisotropy of the thermal expansion of the carboboride was revealed. Along the c axis the coefficient of thermal expansion monotonically increases; in the basal plane, the expansion is practically not observed. The temperature dependence of the unit cell volume Vu(T) has been analyzed in the Debye-Einstein approximation taking into account the electronic contribution and effect of two-level systems. The values of the Gruneisen parameters corresponding to different modes of the phonon spectrum of the carboboride have been determined. The frequencies of the lattice vibrations, determined in a Raman scattering experiment, are in satisfactory agreement with the parameters obtained from Cp(T) using the Debye-Einstein approximation. Using ab initio band theory methods and an exchange-correlation functional in the PBE form in the VASP package, it was established that the total energies of these two crystal structures differ by no more than 0.01 eV f.u.-1. Calculations of the thermodynamic properties of LuB2C yielded similar results for orthorhombic and tetragonal phases of the carboboride.

Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells.

We studied the influence of magnetite nanoparticles (FeO•Fe2O3) and quantum dots (CdSe/ZnS coated with mercaptopropionic acid) on the expression of 5 common reference genes (BA, B2M, PPIA, UBC, and YWHAZ) in peripheral blood cells from 20 volunteers by reverse transcription PCR method. The stability of the expression of reference genes varied depending of the cells type and chemical structure of nanoparticles. The level of YWHAZ mRNA after exposure by nanoparticles demonstrated highest stability in lymphocytes, neutrophils, and monocytes. Stability of YWHAZ expression was confirmed by Western blotting. Our findings suggest that YWHAZ is the most suitable as the reference gene.

Effect of the cation sublattice composition of tin-based type-I clathrates on their low-temperature thermal properties.

We performed an experimental study on thermal properties of the Sn18In6As21.5I8 clathrate by measuring temperature dependencies of its heat capacity (2-300 K) and thermal expansion (5-300 K). By comparing the results with those published previously for Sn-based clathrates Sn24P19.2I8, Sn20Zn4P20.8I8, and Sn17Zn7P22I8, we established that partial replacement of tin and phosphorus by heavier indium and arsenic, respectively, leads to lowering vibration frequencies in both host and guest substructures. Deviation of the observed thermal properties at low temperatures from those predicted by the Einstein-Debye model is caused by the Schottky-like contribution of two-level systems to heat capacity and thermal expansion. These systems form owing to transitions of guest atoms in non-spherical 24-vertex cages between stationary states with close energies.

Dynamics of the crystal structure of tin-based type-I clathrates with different degrees of disorder in their cationic frameworks.

The temperature dependencies of heat capacity, CP(T), and cubic unit cell parameter, a(T), were experimentally obtained in the range of 2-300 K for the compounds Sn24P19.2I8, Sn20Zn4P20.8I8, and Sn17Zn7P22I8, which belong to a family of type-I clathrates. The experimental data were analyzed in the frames of the Debye-Einstein approximation, further accounting for the contributions of positional disorder in the clathrate frameworks as well as those of defect modes arising from the distribution of guest atoms over unequal in energy but close in space positions inside the framework cages. By fitting the experimental data, the Debye and Einstein characteristic temperatures describing the dynamics of the framework and guest atoms, respectively, were obtained. Their analysis revealed peculiar dependencies of the characteristic temperatures upon the number of substituted zinc atoms and the concentration of vacancies in the framework, which are discussed in this paper.

Structural irregularities and peculiarities of low-temperature thermal properties of Sn24P19.4Br8 clathrate.

Temperature changes of the heat capacity and unit cell parameters of Sn24P19.4Br8 clathrate were experimentally determined in the temperature range of 2 to 300 K. The data obtained were analyzed using Debye-Einstein approximation and taking into account the impact of both disorder in the host matrix and the presence of vacancies in the framework. Anomalous negative contribution to the thermal expansion was revealed and related to the defect mode influence on the clathrate thermal properties as a result of vibrations of two-level systems (TLS). The guest atoms that have the opportunity to occupy spatially close yet energetically non-equivalent positions in the asymmetric environment of the host matrix atoms play a principal role in the TLS formation. The results are compared with those previously obtained for semiclathrate Ge31P15Se8.

[Cancer-testis genes in colon cancer]. / Rakovo-testikuliarnye geny pri rake tolstoi kishki.

The expression of cancer-testis (CT) genes varies with tumor type. There are tumors with high, low, and intermediate gene expressions. Tumor cells of different origin are characterized by ST gene co-expression. The expression of ST genes increases in later stages of tumor development in the presence of metastases. In colon cancer, the tumor samples showed most frequently MAGE-A and SSX mRNA. The peripheral blood samples displayed most commonly XAGE, MAGE-C, and SSX mRNA. In patients with colon cancer, the expression of TSP50, MAGE-A(1-6), and SSX1,2,4 genes was associated with a poor prognosis, that of MAGE-C1 and XAGE1 was related to a favorable prognosis.

[The correlation of expression of MUC1, ICAM1, IL32, FcyR3A and FoxP3 in tumor niduses of patients with breast cancer].

The hypo-glycated mucin I (MUC I) frequently is redundantly expressed in cells of breast carcinoma that is considered as an unfavorable prognostic marker for patients with breast cancer. The purpose of study is to evaluate in tumor areas of patients with breast cancer the interrelationships between expression of MUC I and genes participating in implementation of immune reactions. The technique of polymerase chain reaction with reverse transcription in real-time was applied to investigate expression of genes of MUC I, Fas, ICAMI, FcyR3A, FcyR3B, FoxP3, IL2Ra, IL32 and TNF in tumor areas of 40 patients with breast cancer. The level of mRNA of each of analyzed genes was evaluated relatively to three genes of household. The rate of detection of mRNA MUC I amounted to 80%, mRNA Fas - 95%, mRNA ICAM I - 97.5%, mRNA FcyR3A - 95%, mRNA FcyR3B - 32.5%, mRNA FoxP3 - 85%, mRNA IL2Ra - 87.5%, mRNA IL32 - 75%, mRNA TNF - 95%. The direct correlation was established between levels of mRNA MUC I, ICAM I, IL32, FcyR3A and FoxP3. The most strong relationship was registered between levels of expression of genes MUC I and IL32 (r-0.72; p=0.0001). In comparison with MUC I negative breast carcinoma, MUC I positive breast carcinoma characterized by higher level of mRNA of pro-inflammatory cytokines IL32 and ЕТА, molecules of adhesion ICAM I and molecular marker of T-regulative cells FoxP3. The given profile of expression of genes in tumors of patients with breast cancer demonstrates relationship of expression of MUC I with inflammation and its modulation by T-regulative cells. The effect of expression of analyzed genes on phenotype of cancer cells and course of disease are discussed.