Proteomic Analysis of a Syntrophic Coculture of Syntrophobacter fumaroxidans MPOBT and Geobacter sulfurreducens PCAT.

We established a syntrophic coculture of Syntrophobacter fumaroxidans MPOBT (SF) and Geobacter sulfurreducens PCAT (GS) growing on propionate and Fe(III). Neither of the bacteria was capable of growth on propionate and Fe(III) in pure culture. Propionate degradation by SF provides acetate, hydrogen, and/or formate that can be used as electron donors by GS with Fe(III) citrate as electron acceptor. Proteomic analyses of the SF-GS coculture revealed propionate conversion via the methylmalonyl-CoA (MMC) pathway by SF. The possibility of interspecies electron transfer (IET) via direct (DIET) and/or hydrogen/formate transfer (HFIT) was investigated by comparing the differential abundance of associated proteins in SF-GS coculture against (i) SF coculture with Methanospirillum hungatei (SF-MH), which relies on HFIT, (ii) GS pure culture growing on acetate, formate, hydrogen as propionate products, and Fe(III). We noted some evidence for DIET in the SF-GS coculture, i.e., GS in the coculture showed significantly lower abundance of uptake hydrogenase (43-fold) and formate dehydrogenase (45-fold) and significantly higher abundance of proteins related to acetate metabolism (i.e., GltA; 62-fold) compared to GS pure culture. Moreover, SF in the SF-GS coculture showed significantly lower abundance of IET-related formate dehydrogenases, Fdh3 (51-fold) and Fdh5 (29-fold), and the rate of propionate conversion in SF-GS was 8-fold lower than in the SF-MH coculture. In contrast, compared to GS pure culture, we found lower abundance of pilus-associated cytochrome OmcS (2-fold) and piliA (5-fold) in the SF-GS coculture that is suggested to be necessary for DIET. Furthermore, neither visible aggregates formed in the SF-GS coculture, nor the pili-E of SF (suggested as e-pili) were detected. These findings suggest that the IET mechanism is complex in the SF-GS coculture and can be mediated by several mechanisms rather than one discrete pathway. Our study can be further useful in understanding syntrophic propionate degradation in bioelectrochemical and anaerobic digestion systems.

Cortical ChAT+ neurons co-transmit acetylcholine and GABA in a target- and brain-region-specific manner.

The mouse cerebral cortex contains neurons that express choline acetyltransferase (ChAT) and are a potential local source of acetylcholine. However, the neurotransmitters released by cortical ChAT+ neurons and their synaptic connectivity are unknown. We show that the nearly all cortical ChAT+ neurons in mice are specialized VIP+ interneurons that release GABA strongly onto other inhibitory interneurons and acetylcholine sparsely onto layer 1 interneurons and other VIP+/ChAT+ interneurons. This differential transmission of ACh and GABA based on the postsynaptic target neuron is reflected in VIP+/ChAT+ interneuron pre-synaptic terminals, as quantitative molecular analysis shows that only a subset of these are specialized to release acetylcholine. In addition, we identify a separate, sparse population of non-VIP ChAT+ neurons in the medial prefrontal cortex with a distinct developmental origin that robustly release acetylcholine in layer 1. These results demonstrate both cortex-region heterogeneity in cortical ChAT+ interneurons and target-specific co-release of acetylcholine and GABA.

Endozoochorous dispersal of forest seeds by carnivorous mammals in Sierra Fría, Aguascalientes, Mexico.

Some carnivorous mammals ingest fruit and disperse seeds of forest plant species capable of colonizing disturbed areas in ecosystems. The objective of the present study was to evaluate the dissemination of Arctostaphylos pungens and Juniperus deppeana seeds by the gray fox (Urocyon cinereoargenteus), coyote (Canis latrans), and other carnivores in the Protected Natural Area Sierra Fría, in Aguascalientes, Mexico. Scat collection was undertaken via transects using the direct search method, while the seasonal phenology of A. pungens and J. deppeana was evaluated by recording flower and fruit abundance on both the plant and the surrounding forest floor ground. Seed viability was assessed by optical densitometry via X-ray and a germination test. It was found that the gray fox, coyote, ringtail (Bassariscus astutus), and bobcat (Lynx rufus) disseminated seeds of A. pungens (212 ± 48.9 seeds/scat) and J. deppeana (23.6 ± 4.9 seeds/scat), since a large proportion of the collected scat of these species contained seeds (28/30 = 93.33%, 12/43 = 27.9%, 6/12 = 50% and 7/25 = 28% respectively). The gray fox, coyote, ringtail, and bobcat presented an average of seed dispersion of both plant species of 185.4 ± 228.7, 4.0 ± 20.0, 12.1 ± 30.4, and 0.8 ± 1.5 per scat; the seed proportions in the gray fox, coyote, ringtail, and bobcat were 89.6/10.4%, 82.3/17.7%, 90.4/9.6%, and 38.1/61.9% for A. pungens and J. deppeana, respectively. The phenology indicated a finding related to the greater abundance of ripe fruit in autumn and winter (p < .01). This coincided with the greater abundance of seeds found in scats during these seasons. Endozoochory and diploendozoochory enhanced the viability and germination of the seeds (p > .05), except in those of A. pungens dispersed by coyote. These results suggest that carnivores, particularly the gray fox, the coyote, and the bobcat, play an important role in forest seed dissemination, and thus forest regeneration, by making both a quantitative and qualitative contribution to the dispersal of the two pioneer species under study.

Caveolae in CNS arterioles mediate neurovascular coupling.

Proper brain function depends on neurovascular coupling: neural activity rapidly increases local blood flow to meet moment-to-moment changes in regional brain energy demand1. Neurovascular coupling is the basis for functional brain imaging2, and impaired neurovascular coupling is implicated in neurodegeneration1. The underlying molecular and cellular mechanisms of neurovascular coupling remain poorly understood. The conventional view is that neurons or astrocytes release vasodilatory factors that act directly on smooth muscle cells (SMCs) to induce arterial dilation and increase local blood flow1. Here, using two-photon microscopy to image neural activity and vascular dynamics simultaneously in the barrel cortex of awake mice under whisker stimulation, we found that arteriolar endothelial cells (aECs) have an active role in mediating neurovascular coupling. We found that aECs, unlike other vascular segments of endothelial cells in the central nervous system, have abundant caveolae. Acute genetic perturbations that eliminated caveolae in aECs, but not in neighbouring SMCs, impaired neurovascular coupling. Notably, caveolae function in aECs is independent of the endothelial NO synthase (eNOS)-mediated NO pathway. Ablation of both caveolae and eNOS completely abolished neurovascular coupling, whereas the single mutants exhibited partial impairment, revealing that the caveolae-mediated pathway in aECs is a major contributor to neurovascular coupling. Our findings indicate that vasodilation is largely mediated by endothelial cells that actively relay signals from the central nervous system to SMCs via a caveolae-dependent pathway.

Cholesterol granuloma in an antrochoanal polyp. A rare lesion in children / Granuloma por colesterol en un pólipo antrocoanal. Una rara lesión en la edad pediátrica

Antrochoanal polyps (ACPs) are an infrequent clinical entity. Cholesterol granulomas (CGs) are commonly associated with chronic middle ear disease but are rare in the paranasal sinuses. We describe a case of a 10-year-old girl with a concomitant CG in an ACP which was surgically excised by nasosinusal endoscopic surgery. To our knowledge, there are only five previously published cases of CGs in ACPs and of these, only two were pediatric cases. We describe a third case in the youngest patient yet reported. Increased intrasinus pressure may affect venous and lymphatic drainage, leading to hemorrhages with hemolysis and deposition of cholesterol crystals and their esters initiating the formation of granulomas in the polyp. In addition, the insufficient lymphatic drainage prevents the complete elimination of lipids, contributing to the formation of cholesterol granulomas. The treatment and the outcome of an ACP associated with a CG are the same as for usual ACPs

Los pólipos antrocoanales (PAC) son una entidad clínica infrecuente. Los granulomas de colesterol (GC) se asocian comúnmente con la enfermedad crónica del oído medio, pero son raros en los senos paranasales. En este artículo describimos un caso de GC concomitante con un PAC de una niña de 10 años que fue sometida a extirpación quirúrgica nasosinusal endoscópica. En nuestro conocimiento, solamente 5 casos de GC asociados a PAC han sido publicados. De ellos solamente 2 eran casos pediátricos. En el presente artículo describimos un tercer caso que representa el paciente más joven publicado hasta la fecha. El aumento de la presión en la cavidad del seno puede afectar al drenaje venoso y linfático conduciendo a hemorragias con hemólisis y depósito de cristales de colesterol y sus ésteres iniciando la formación de granulomas en el pólipo. Además, el drenaje linfático insuficiente impide la eliminación completa de los lípidos contribuyendo a la formación de granulomas por colesterol. El tratamiento y la evolución del PAC asociado a GC son similares a los del PAC habitual

Cholesterol granuloma in an antrochoanal polyp. A rare lesion in children.

Antrochoanal polyps (ACPs) are an infrequent clinical entity. Cholesterol granulomas (CGs) are commonly associated with chronic middle ear disease but are rare in the paranasal sinuses. We describe a case of a 10-year-old girl with a concomitant CG in an ACP which was surgically excised by nasosinusal endoscopic surgery. To our knowledge, there are only five previously published cases of CGs in ACPs and of these, only two were pediatric cases. We describe a third case in the youngest patient yet reported. Increased intrasinus pressure may affect venous and lymphatic drainage, leading to hemorrhages with hemolysis and deposition of cholesterol crystals and their esters initiating the formation of granulomas in the polyp. In addition, the insufficient lymphatic drainage prevents the complete elimination of lipids, contributing to the formation of cholesterol granulomas. The treatment and the outcome of an ACP associated with a CG are the same as for usual ACPs.

Comparative proteome analysis of propionate degradation by Syntrophobacter fumaroxidans in pure culture and in coculture with methanogens.

Syntrophobacter fumaroxidans is a sulfate-reducing bacterium able to grow on propionate axenically or in syntrophic interaction with methanogens or other sulfate-reducing bacteria. We performed a proteome analysis of S. fumaroxidans growing with propionate axenically with sulfate or fumarate, and in syntrophy with Methanospirillum hungatei, Methanobacterium formicicum or Desulfovibrio desulfuricans. Special attention was put on the role of hydrogen and formate in interspecies electron transfer (IET) and energy conservation. Formate dehydrogenase Fdh1 and hydrogenase Hox were the main confurcating enzymes used for energy conservation. In the periplasm, Fdh2 and hydrogenase Hyn play an important role in reverse electron transport associated with succinate oxidation. Periplasmic Fdh3 and Fdh5 were involved in IET. The sulfate reduction pathway was poorly regulated and many enzymes associated with sulfate reduction (Sat, HppA, AprAB, DsrAB and DsrC) were abundant even at conditions where sulfate was not present. Proteins similar to heterodisulfide reductases (Hdr) were abundant. Hdr/Flox was detected in all conditions while HdrABC/HdrL was exclusively detected when sulfate was available; these complexes most likely confurcate electrons. Our results suggest that S. fumaroxidans mainly used formate for electron release and that different confurcating mechanisms were used in its sulfidogenic metabolism.

Erythrocyte-derived nano-probes functionalized with antibodies for targeted near infrared fluorescence imaging of cancer cells.

Constructs derived from mammalian cells are emerging as a new generation of nano-scale platforms for clinical imaging applications. Herein, we report successful engineering of hybrid nano-structures composed of erythrocyte-derived membranes doped with FDA-approved near infrared (NIR) chromophore, indocyanine green (ICG), and surface-functionalized with antibodies to achieve molecular targeting. We demonstrate that these constructs can be used for targeted imaging of cancer cells in vitro. These erythrocyte-derived optical nano-probes may provide a potential platform for clinical translation, and enable molecular imaging of cancer biomarkers.

Discovery of Small Molecules for Fluorescent Detection of Complement Activation Product C3d.

Complement activation plays a major role in many acute and chronic inflammatory conditions. C3d, a terminal product of complement activation, remains covalently attached to cells and is an excellent biomarker of complement-mediated inflammation. We employed a virtual high-throughput screening protocol to identify molecules with predicted binding to complement C3d and with intrinsic fluorescence properties to enable detection. Pharmacophore models were developed based on known C3d-ligand interactions and information from computational analysis of structural and molecular dynamics data. Iterative pharmacophore-based virtual screening was performed to identify druglike molecules with physicochemical similarity to the natural C3d ligand CR2. Hits from the pharmacophore screens were docked to C3d and ranked based on predicted binding free energies. Top-ranked molecules were selected for experimental validation of binding affinity to C3d, using microscale thermophoresis, and for their suitability to become molecular imaging agents, using fluorescence spectroscopy. This work serves as a foundation for identifying additional fluorescent molecules with high-affinity for C3d that will subsequently be explored as noninvasive in vivo diagnostics of complement-mediated inflammation, for spatiotemporal monitoring of disease progression, and for targeting therapeutics to sites of inflammation.

Adenocarcinoma de cérvix en el segundo trimestre de embarazo / Cervical adenocarcinoma in the second trimester of pregnancy

El cáncer de cérvix es una de las neoplasias diagnosticadas con más frecuencia durante la gestación. El manejo de estos casos será individualizado y basado en la edad gestacional en el momento del diagnóstico, el estadio tumoral y el deseo de la paciente de continuar o no con el embarazo. Presentamos el caso de un adenocarcinoma de cérvix diagnosticado en la semana 18 de gestación, en el que se decidió un manejo expectante (AU)

Cervical cancer is one of the most common malignancies diagnosed during pregnancy. The management of these patients is individualized and is based on gestational age at diagnosis, tumor stage, and the patient's desire to continue the pregnancy or not. We report a case of adenocarcinoma of the cervix that was diagnosed in the 18th week of pregnancy, in which the approach adopted was expectant management (AU)

Photoinduced dynamics of a cyanine dye: parallel pathways of non-radiative deactivation involving multiple excited-state twisted transients.

Cyanine dyes are broadly used for fluorescence imaging and other photonic applications. 3,3'-Diethylthiacyanine (THIA) is a cyanine dye composed of two identical aromatic heterocyclic moieties linked with a single methine, -CH[double bond, length as m-dash]. The torsional degrees of freedom around the methine bonds provide routes for non-radiative decay, responsible for the inherently low fluorescence quantum yields. Using transient absorption spectroscopy, we determined that upon photoexcitation, the excited state relaxes along two parallel pathways producing three excited-state transients that undergo internal conversion to the ground state. The media viscosity impedes the molecular modes of ring rotation and preferentially affects one of the pathways of non-radiative decay, exerting a dominant effect on the emission properties of THIA. Concurrently, the polarity affects the energy of the transients involved in the decay pathways and further modulates the kinetics of non-radiative deactivation.

Correction: Photoinduced dynamics of a cyanine dye: parallel pathways of non-radiative deactivation involving multiple excited-state twisted transients.

[This corrects the article DOI: 10.1039/C4SC02881C.].

Dipole-mediated rectification of intramolecular photoinduced charge separation and charge recombination.

Controlling charge transfer at a molecular scale is critical for efficient light harvesting, energy conversion, and nanoelectronics. Dipole-polarization electrets, the electrostatic analogue of magnets, provide a means for "steering" electron transduction via the local electric fields generated by their permanent electric dipoles. Here, we describe the first demonstration of the utility of anthranilamides, moieties with ordered dipoles, for controlling intramolecular charge transfer. Donor-acceptor dyads, each containing a single anthranilamide moiety, distinctly rectify both the forward photoinduced electron transfer and the subsequent charge recombination. Changes in the observed charge-transfer kinetics as a function of media polarity were consistent with the anticipated effects of the anthranilamide molecular dipoles on the rectification. The regioselectivity of electron transfer and the molecular dynamics of the dyads further modulated the observed kinetics, particularly for charge recombination. These findings reveal the underlying complexity of dipole-induced effects on electron transfer and demonstrate unexplored paradigms for molecular rectifiers.

A genomic view on syntrophic versus non-syntrophic lifestyle in anaerobic fatty acid degrading communities.

In sulfate-reducing and methanogenic environments complex biopolymers are hydrolyzed and degraded by fermentative micro-organisms that produce hydrogen, carbon dioxide and short chain fatty acids. Degradation of short chain fatty acids can be coupled to methanogenesis or to sulfate-reduction. Here we study from a genome perspective why some of these micro-organisms are able to grow in syntrophy with methanogens and others are not. Bacterial strains were selected based on genome availability and upon their ability to grow on short chain fatty acids alone or in syntrophic association with methanogens. Systematic functional domain profiling allowed us to shed light on this fundamental and ecologically important question. Extra-cytoplasmic formate dehydrogenases (InterPro domain number; IPR006443), including their maturation protein FdhE (IPR024064 and IPR006452) is a typical difference between syntrophic and non-syntrophic butyrate and propionate degraders. Furthermore, two domains with a currently unknown function seem to be associated with the ability of syntrophic growth. One is putatively involved in capsule or biofilm production (IPR019079) and a second in cell division, shape-determination or sporulation (IPR018365). The sulfate-reducing bacteria Desulfobacterium autotrophicum HRM2, Desulfomonile tiedjei and Desulfosporosinus meridiei were never tested for syntrophic growth, but all crucial domains were found in their genomes, which suggests their possible ability to grow in syntrophic association with methanogens. In addition, profiling domains involved in electron transfer mechanisms revealed the important role of the Rnf-complex and the formate transporter in syntrophy, and indicate that DUF224 may have a role in electron transfer in bacteria other than Syntrophomonas wolfei as well. This article is a part of a Special Issue entitled: 18th European Bioenergetics Conference (Biochim. Biophys. Acta, Volume 1837, Issue 7, July 2014).

Degradation and antibacterial properties of magnesium alloys in artificial urine for potential resorbable ureteral stent applications.

This article presents an investigation on the effectiveness of magnesium and its alloys as a novel class of antibacterial and biodegradable materials for ureteral stent applications. Magnesium is a lightweight and biodegradable metallic material with beneficial properties for use in medical devices. Ureteral stent is one such example of a medical device that is widely used to treat ureteral canal blockages clinically. The bacterial colony formation coupled with the encrustation on the stent surface from extended use often leads to clinical complications and contributes to the failure of indwelling medical devices. We demonstrated that magnesium alloys decreased Escherichia coli viability and reduced the colony forming units over a 3-day incubation period in an artificial urine (AU) solution when compared with currently used commercial polyurethane stent. Moreover, the magnesium degradation resulted in alkaline pH and increased magnesium ion concentration in the AU solution. The antibacterial and degradation properties support the potential use of magnesium-based materials for next-generation ureteral stents. Further studies are needed for clinical translation of biodegradable metallic ureteral stents.

Microfluidic space-domain time-resolved emission spectroscopy of terbium(III) and europium(III) chelates with pyridine-2,6-dicarboxylate.

This article describes the utilization of laminar microflows for time-resolved emission measurements with steady-state excitation and detection. Passing a laminar flow through a short illuminated section of a microchannel provided a means for pulsed-like photoexcitation of the moieties carried by the fluid. Imaging the microchannel flows carrying thus photoexcited chelates of lanthanide ions allowed us to extract their excited-state lifetimes from the spatial distribution of the changes in the emission intensity. The lifetime values obtained using this space-domain approach agreed well with the lifetimes from time-domain measurements. This validated space-domain microfluidic approach reveals a means for miniaturization of time-resolved emission spectroscopy.

Amyloid histology stain for rapid bacterial endospore imaging.

Bacterial endospores are some of the most resilient forms of life known to us, with their persistent survival capability resulting from a complex and effective structural organization. The outer membrane of endospores is surrounded by the densely packed endospore coat and exosporium, containing amyloid or amyloid-like proteins. In fact, it is the impenetrable composition of the endospore coat and the exosporium that makes staining methodologies for endospore detection complex and challenging. Therefore, a plausible strategy for facile and expedient staining would be to target components of the protective surface layers of the endospores. Instead of targeting endogenous markers encapsulated in the spores, here we demonstrated staining of these dormant life entities that targets the amyloid domains, i.e., the very surface components that make the coats of these species impenetrable. Using an amyloid staining dye, thioflavin T (ThT), we examined this strategy. A short incubation of bacillus endospore suspensions with ThT, under ambient conditions, resulted in (i) an enhancement of the fluorescence of ThT and (ii) the accumulation of ThT in the endospores, affording fluorescence images with excellent contrast ratios. Fluorescence images revealed that ThT tends to accumulate in the surface regions of the endospores. The observed fluorescence enhancement and dye accumulation, coupled with the sensitivity of emission techniques, provide an effective and rapid means of staining endospores without the inconvenience of pre- or posttreatment of samples.

Kinetics of bacterial fluorescence staining with 3,3'-diethylthiacyanine.

For more than a century, colorimetric and fluorescence staining have been the foundation of a broad range of key bioanalytical techniques. The dynamics of such staining processes, however, still remains largely unexplored. We investigated the kinetics of fluorescence staining of two gram-negative and two gram-positive species with 3,3'-diethylthiacyanine (THIA) iodide. An increase in the THIA fluorescence quantum yield, induced by the bacterial dye uptake, was the principal reason for the observed emission enhancement. The fluorescence quantum yield of THIA depended on the media viscosity and not on the media polarity, which suggested that the microenvironment of the dye molecules taken up by the cells was restrictive. The kinetics of fluorescence staining did not manifest a statistically significant dependence neither on the dye concentration, nor on the cell count. In the presence of surfactant additives, however, the fluorescence-enhancement kinetic patterns manifested species specificity with statistically significant discernibility.

Leiomioma vulvar atípico / Atypical vulvar leiomyoma

Los criterios valorados para el diagnóstico de malignidad en los tumores de músculo liso primarios vulvares son: tamaño ≥ 5 cm, márgenes infiltrantes, 5 o más mitosis por cada 10 campos de gran aumento, y atipia citológica moderada o grave. Con 3 o más de estos criterios, el diagnóstico será de leiomiosarcoma, y con uno o menos, de leiomioma. Cuando el tumor reúne sólo 2 criterios, el diagnóstico es de leiomioma atípico o leiomiosarcoma de bajo grado, y presenta un riesgo de recidiva local más que de enfermedad metastásica. Presentamos un caso de leiomioma atípico vulvar en una paciente de 87 años de edad, de 6,5 cm de eje máximo y de 5-7 mitosis por cada 10 campos de gran aumento, a la que se realizó un tratamiento conservador y un seguimiento de la evolución

The evaluation criteria for the diagnosis of primary malignant smooth-muscle tumors of the vulva are size equal to or greater than 5 cm, infiltrating margins, more than 5 mitotic figures per 10 high-power fields, and moderate to severe cytologic atypia. Lesions with three or more of these features should be diagnosed as leiomyosarcoma while those with only one of these characteristics should be diagnosed as leiomyoma. Lesions with two of those features should be considered as atypical leiomyoma or low-grade leiomyosarcoma, with a risk of local recurrence rather than of metastatic disease. We report the case of an 87-year-old woman with an atypical leiomyoma of the vulva. Histological characterization of the tumor revealed a maximum diameter of 6.5 cm and 5-7 mitotic figures per 10 high-power fields. The patient underwent conservative treatment and follow-up

Incidencia y mortalidad del cáncer epitelial de ovario en el Área Sanitaria de León / Incidence and mortality of ovarian epithelial cancer in the health area of León (Spain)

Objetivo: Analizar la incidencia y la mortalidad por cáncer epitelial de ovario en el Área Sanitaria de León durante el período 1991-2001. Pacientes y métodos: Incluimos en el estudio a 236 mujeres con diagnóstico histológico de cáncer epitelial, invasivo, de ovario. Se calcularon las tasas brutas, acumuladas, truncadas, específicas por edad y ajustadas a la población mundial, tanto de la incidencia como de la mortalidad. Resultados: La tasa bruta de incidencia en todo el período es de 11,07 x 105 mujeres al año, la tasa ajustada de 5,97 x 105 mujeres al año, y la tasa acumulada, que indica el riesgo que tiene una mujer de esta área sanitaria de presentar un cáncer de ovario a lo largo de su vida, es de 0,15, es decir, 1,5 de cada 1.000 mujeres tendrán esta enfermedad. La tasa bruta de mortalidad es de 6,15 x 105 mujeres al año; la tasa ajustada, de 2,70 x 105 mujeres al año y la tasa acumulada de mortalidad, que indica el riesgo de morir por esta enfermedad, es de 1,39. Conclusiones: Las tasas de incidencia son similares o ligeramente superiores a las cifras publicadas para todo el territorio nacional; sin embargo, la tasa de mortalidad es menor que en otras provincias españolas y el conjunto de los países europeos, coincidiendo con las tasas de mortalidad apreciadas en el grupo de los países mediterráneos

Aim: To analyze the incidence and mortality of ovarian epithelial cancer in the health area of León between 1991 and 2000. Patients and methods: A total of 236 women with a histodiagnosis of invasive ovarian epithelial cancer were included in this study. We calculated the crude, accumulated, truncated, and age-specific incidence and mortality rates adjusted to the world population. Results: The crude incidence rate for the entire period was 11.07 x 105 women/year, the adjusted rate was 5.97 x 105 women/year, and the accumulated rate, which indicates the risk of developing ovarian cancer over a lifespan in women from this health area, was 0.15, that is, 1.5 out of every 1000 women will suffer from this disease. The crude mortality rate was 6.15 x 105 women/year, the adjusted rate was 2.70 x 105 women/year, and the accumulated mortality rate, which indicates the risk of dying from this disease, was 1.39. Conclusions: The incidence rates in this study are similar or slightly higher than those reported for Spain as a whole. However, the mortality rate is lower than that in other Spanish provinces and in the European Union as a whole and is similar to mortality rates in the group of Mediterranean countries