Structural advances in transcription elongation.

Transcription is the first step of gene expression and involves RNA polymerases. After transcription initiation, RNA polymerase enters elongation followed by transcription termination at the end of the gene. Only recently, structures of transcription elongation complexes bound to key transcription elongation factors have been determined in bacterial and eukaryotic systems. These structures have revealed numerous insights including the basis for transcriptional pausing, RNA polymerase interaction with large complexes such as the ribosome and the spliceosome, and the transition into productive elongation. Here, we review these structures and describe areas for future research.

Mathematical modeling of the lower urinary tract: A review.

AIMS: Understand what progress has been made toward a functionally predictive lower urinary tract (LUT) model, identify knowledge gaps, and develop from them a path forward. METHODS: We surveyed prominent mathematical models of the basic LUT components (bladder, urethra, and their neural control) and categorized the common modeling strategies and theoretical assumptions associated with each component. Given that LUT function emerges from the interaction of these components, we emphasized attempts to model their connections, and highlighted unmodeled aspects of LUT function. RESULTS: There is currently no satisfactory model of the LUT in its entirety that can predict its function in response to disease, treatment, or other perturbations. In particular, there is a lack of physiologically based mathematical descriptions of the neural control of the LUT. CONCLUSIONS: Based on our survey of the work to date, a potential path to a predictive LUT model is a modular effort in which models are initially built of individual tissue-level components using methods that are extensible and interoperable, allowing them to be connected and tested in a common framework. A modular approach will allow the larger goal of a comprehensive LUT model to be in sight while keeping individual efforts manageable, ensure new models can straightforwardly build on prior research, respect potential interactions between components, and incentivize efforts to model absent components. Using a modular framework and developing models based on physiological principles, to create a functionally predictive model is a challenge that the field is ready to undertake.

Occupational Exposure of Hairdressers to Airborne Hazardous Chemicals: A Scoping Review.

INTRODUCTION: Exposure to hazardous chemicals released during hairdressing activities from hair care products puts hairdressers at risk of adverse health effects. Safety assessments of hair products are mainly focused on consumers, but exposure for professional hairdressers might be substantially higher. OBJECTIVE: To identify and assess available research data on inhalation exposures of professional hairdressers. METHODS: A systematic search of studies between 1 January 2000 and 30 April 2021 was performed in Medline, Embase, Web of Science and in Cochrane registry, toxicological dossiers of the Scientific Committee on Consumer Safety (SCCS) of the European Commission as well as the German MAK Commission. Studies reporting quantitative data on airborne concentrations of chemicals in the hairdresser's workplace were considered. The outcome was an airborne concentration of chemicals in the working environment, which was compared, when possible, with current occupational exposure limits (OEL) or guidance levels. RESULTS: In total, 23 studies performed in 14 countries were included. The average number of hairdressing salons per study was 22 (range 1-62). Chemicals most frequently measured were formaldehyde (n = 8), ammonia (n = 5), total volatile organic compounds (TVOC) (n = 5), and toluene (n = 4). More than fifty other chemicals were measured in one to three studies, including various aromatic and aliphatic organic solvents, hydrogen peroxide, persulfate, and particulate matter. Most studies reported environmental air concentrations, while personal exposure was measured only in seven studies. The measured air concentrations of formaldehyde, ammonia, and TVOC exceeded OEL or guidance values in some studies. There was large variability in measuring conditions and reported air concentrations differed strongly within and between studies. CONCLUSION: Hairdressers are exposed to a wide spectrum of hazardous chemicals, often simultaneously. Airborne concentrations of pollutants depend on salon characteristics such as ventilation and the number of customers but also on used products that are often country- or client-specific. For exposure to formaldehyde, ammonia, and TVOC exceeding OELs or guidance values for indoor air was observed. Therefore, occupational exposure should be taken into account by safety regulations for hair care products.

Artificial Intelligence to Aid Glaucoma Diagnosis and Monitoring: State of the Art and New Directions.

Recent developments in the use of artificial intelligence in the diagnosis and monitoring of glaucoma are discussed. To set the context and fix terminology, a brief historic overview of artificial intelligence is provided, along with some fundamentals of statistical modeling. Next, recent applications of artificial intelligence techniques in glaucoma diagnosis and the monitoring of glaucoma progression are reviewed, including the classification of visual field images and the detection of glaucomatous change in retinal nerve fiber layer thickness. Current challenges in the direct application of artificial intelligence to further our understating of this disease are also outlined. The article also discusses how the combined use of mathematical modeling and artificial intelligence may help to address these challenges, along with stronger communication between data scientists and clinicians.

Bottom-up reconstitution of focal adhesion complexes.

Focal adhesions (FA) are large macromolecular assemblies relevant for various cellular and pathological events such as migration, polarization, and metastatic cancer formation. At FA sites at the migrating periphery of a cell, hundreds of players gather and form a network to respond to extra cellular stimuli transmitted by the integrin receptor, the most upstream component within a cell, initiating the FA signaling pathway. Numerous cellular experiments have been performed to understand the FA architecture and functions; however, their intricate network formation hampers unraveling the precise molecular actions of individual players. Here, in vitro bottom-up reconstitution presents an advantageous approach for elucidating the FA machinery and the hierarchical crosstalk of involved cellular players.

Structural insights into integrin α5ß1 opening by fibronectin ligand.

Integrin α5ß1 is a major fibronectin receptor critical for cell migration. Upon complex formation, fibronectin and α5ß1 undergo conformational changes. While this is key for cell-tissue connections, its mechanism is unknown. Here, we report cryo-electron microscopy structures of native human α5ß1 with fibronectin to 3.1-angstrom resolution, and in its resting state to 4.6-angstrom resolution. The α5ß1-fibronectin complex revealed simultaneous interactions at the arginine-glycine-aspartate loop, the synergy site, and a newly identified binding site proximal to adjacent to metal ion-dependent adhesion site, inducing the translocation of helix α1 to secure integrin opening. Resting α5ß1 adopts an incompletely bent conformation, challenging the model of integrin sharp bending inhibiting ligand binding. Our biochemical and structural analyses showed that affinity of α5ß1 for fibronectin is increased with manganese ions (Mn2+) while adopting the half-bent conformation, indicating that ligand-binding affinity does not depend on conformation, and α5ß1 opening is induced by ligand-binding.

Gradual opening of Smc arms in prokaryotic condensin.

Multi-subunit SMC ATPases control chromosome superstructure apparently by catalyzing a DNA-loop-extrusion reaction. SMC proteins harbor an ABC-type ATPase "head" and a "hinge" dimerization domain connected by a coiled coil "arm." Two arms in a SMC dimer can co-align, thereby forming a rod-shaped particle. Upon ATP binding, SMC heads engage, and arms are thought to separate. Here, we study the shape of Bacillus subtilis Smc-ScpAB by electron-spin resonance spectroscopy. Arm separation is readily detected proximal to the heads in the absence of ligands, and separation near the hinge largely depends on ATP and DNA. Artificial blockage of arm opening eliminates DNA stimulation of ATP hydrolysis but does not prevent basal ATPase activity. We report an arm contact as being important for controlling the transformations. Point mutations at this arm interface eliminated Smc function. We propose that partially open, intermediary conformations provide directionality to SMC DNA translocation by (un)binding suitable DNA substrates.

Marine Seagrass Extract of Thalassia testudinum Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways.

Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm Thalassia testudinum (TTE) in colon tumor cell lines (RKO, SW480, and CT26) and a syngeneic allograft murine colorectal cancer model. MTT assays revealed a dose-dependent decrease of cell viability of RKO, CT26, and SW480 cells upon TTE treatment with IC50 values of, respectively, 175, 115, and 60 µg/mL. Furthermore, TTE significantly prevented basal and bFGF-induced angiogenesis in the chicken chorioallantoic membrane angiogenesis assay. In addition, TTE suppressed bFGF-induced migration of endothelial cells in a wound closure assay. Finally, TTE treatment abrogated CT26 colorectal cancer growth and increased overall organism survival in a syngeneic murine allograft model. Corresponding transcriptome profiling and pathway analysis allowed for the identification of the mechanism of action for the antitumor effects of TTE. In line with our in vitro/in vivo results, TTE treatment triggers ATF4-P53-NFκB specific gene expression and autophagy stress pathways. This results in suppression of colon cancer cell growth, cell motility, and angiogenesis pathways in vitro and in addition promotes antitumor immunogenic cell death in vivo.

Docking and Molecular Dynamics Predictions of Pesticide Binding to the Calyx of Bovine ß-Lactoglobulin.

Pesticides are used extensively in agriculture, and their residues in food must be monitored to prevent toxicity. The most abundant protein in cow's milk, ß-lactoglobulin (BLG), shows high affinity for diverse hydrophobic ligands in its central binding pocket, called the calyx. Several of the most frequently used pesticides are hydrophobic. To predict if BLG may be an unintended carrier for pesticides, we tested its ability to bind 555 pesticides and their isomers, for a total of 889 compounds, in a rigid docking screen. We focused on the analysis of 60 unique molecules belonging to the five pesticide classes defined by the World Health Organization, that docked into BLG's calyx with ΔGs ranging from -8.2 to -12 kcal mol-1, chosen by statistical criteria. These "potential ligands" were further analyzed using molecular dynamic simulations, and the binding energies were explored with Molecular Mechanics/Generalized Born/Surface Area (MMGBSA). Hydrophobic pyrethroid insecticides, like cypermethrin, were found to bind as deeply and tightly into the calyx as BLG's natural ligand, palmitate; while polar compounds, like paraquat, were expelled. Our results suggest that BLG could be a carrier for pesticides, in particular for pyrethroid insecticides, allowing for their accumulation in cow's milk beyond their solubility restrictions. This analysis opens possibilities for pesticide biosensor design based on BLG.

Transient DNA Occupancy of the SMC Interarm Space in Prokaryotic Condensin.

Multi-subunit SMC ATPases control chromosome superstructure and DNA topology, presumably by DNA translocation and loop extrusion. Chromosomal DNA is entrapped within the tripartite SMCkleisin ring. Juxtaposed SMC heads ("J heads") or engaged SMC heads ("E heads") split the SMCkleisin ring into "S" and "K" sub-compartments. Here, we map a DNA-binding interface to the S compartment of E heads SmcScpAB and show that head-DNA association is essential for efficient DNA translocation and for traversing highly transcribed genes in Bacillus subtilis. We demonstrate that in J heads, SmcScpAB chromosomal DNA resides in the K compartment but is absent from the S compartment. Our results imply that the DNA occupancy of the S compartment changes during the ATP hydrolysis cycle. We propose that DNA translocation involves DNA entry into and exit out of the S compartment, possibly by DNA transfer between compartments and DNA segment capture.

Tuned SMC Arms Drive Chromosomal Loading of Prokaryotic Condensin.

SMC proteins support vital cellular processes in all domains of life by organizing chromosomal DNA. They are composed of ATPase "head" and "hinge" dimerization domains and a connecting coiled-coil "arm." Binding to a kleisin subunit creates a closed tripartite ring, whose ∼47-nm-long SMC arms act as barrier for DNA entrapment. Here, we uncover another, more active function of the bacterial Smc arm. Using high-throughput genetic engineering, we resized the arm in the range of 6-60 nm and found that it was functional only in specific length regimes following a periodic pattern. Natural SMC sequences reflect these length constraints. Mutants with improper arm length or peptide insertions in the arm efficiently target chromosomal loading sites and hydrolyze ATP but fail to use ATP hydrolysis for relocation onto flanking DNA. We propose that SMC arms implement force transmission upon nucleotide hydrolysis to mediate DNA capture or loop extrusion.

The tertiary origin of the allosteric activation of E. coli glucosamine-6-phosphate deaminase studied by sol-gel nanoencapsulation of its T conformer.

The role of tertiary conformational changes associated to ligand binding was explored using the allosteric enzyme glucosamine-6-phosphate (GlcN6P) deaminase from Escherichia coli (EcGNPDA) as an experimental model. This is an enzyme of amino sugar catabolism that deaminates GlcN6P, giving fructose 6-phosphate and ammonia, and is allosterically activated by N-acetylglucosamine 6-phosphate (GlcNAc6P). We resorted to the nanoencapsulation of this enzyme in wet silica sol-gels for studying the role of intrasubunit local mobility in its allosteric activation under the suppression of quaternary transition. The gel-trapped enzyme lost its characteristic homotropic cooperativity while keeping its catalytic properties and the allosteric activation by GlcNAc6P. The nanoencapsulation keeps the enzyme in the T quaternary conformation, making possible the study of its allosteric activation under a condition that is not possible to attain in a soluble phase. The involved local transition was slowed down by nanoencapsulation, thus easing the fluorometric analysis of its relaxation kinetics, which revealed an induced-fit mechanism. The absence of cooperativity produced allosterically activated transitory states displaying velocity against substrate concentration curves with apparent negative cooperativity, due to the simultaneous presence of subunits with different substrate affinities. Reaction kinetics experiments performed at different tertiary conformational relaxation times also reveal the sequential nature of the allosteric activation. We assumed as a minimal model the existence of two tertiary states, t and r, of low and high affinity, respectively, for the substrate and the activator. By fitting the velocity-substrate curves as a linear combination of two hyperbolic functions with Kt and Kr as KM values, we obtained comparable values to those reported for the quaternary conformers in solution fitted to MWC model. These results are discussed in the background of the known crystallographic structures of T and R EcGNPDA conformers. These results are consistent with the postulates of the Tertiary Two-States (TTS) model.

Una experiencia de capacitación sobre el análisis del trabajo / A training experience in work analysis

Con el propósito de capacitar en un método de análisis del trabajo, a un grupo de estudiantes de postgrado en Salud Ocupacional de la Universidad de Carabobo, Núcleo Aragua, Venezuela, el Centro de Estudios en Salud de los Trabajadores (CEST-UC) desarrolló un programa teórico práctico donde se implementaron un conjunto de técnicas para el análisis del trabajo con un enfoque que pretende ser sistémico y holístico. El programa de capacitación impartido se desarrolló básicamente sobre tres aspectos claves: desarrollo teórico, aspectos metodológicos y desarrollo instrumental. Fueron entrenados cinco profesionales a los cuales se les dio la tarea de facilitadores, quienes monitorizaron los ensayos de intervención. Al curso asistieron 20 profesionales que se dividieron en cinco (5) equipos para la evaluación de diferentes puestos de trabajo en varias dependencias de la Universidad. La descripción de las condiciones de trabajo, las premisas individuales y los efectos negativos evaluados en los trabajadores(as) que se desempeñaban en los puestos estudiados fueron consignados por los equipos participantes a las dependencias, lo que permitió establecer el diagnóstico general de las condiciones de los puestos evaluados así como la frecuencia y magnitud de los efectos negativos a la salud, entre los cuales el mayormente reportado fue la fatiga. El aprovechamiento de los pasantes del curso fue significativo logrando el diagnóstico de los puestos en las dependencias designadas. El Centro de Estudios en Salud de los Trabajadores de la Universidad de Carabobo cuenta con cinco expertos que pueden replicar el curso realizado y multiplicar el conocimiento adquirido.

In the context of a training program in work analysis for occupational health students at the University of Carabobo, state of Aragua, Venezuela, the Center for Studies on Workers’ Health (CEST-UC), developed a theoretical and practical framework using a systemic and holistic approach. The program had three key components: theoretical foundations, methodological aspects, and instrumentation development. Five health professionals were trained as facilitators to oversee the various interventions. Then 20 professionals attending the training program were divided into five teams and evaluated different workplaces in several university departments. The description of working conditions, individual characteristics and both frequency and magnitude of adverse health effects on workers were identified by the teams by departments. Fatigue was the most commonly reported symptom. Engaging training program participants proved very useful in establishing working condition profiles by departments. The Center for Studies on Workers' Health of the University of Carabobo has five experts who can replicate the course and multiply the acquired knowledge.

Concepciones de los maestros sobre la creatividad y su enseñanza / Teachers’ concept about creativity and its teaching

Esta investigación tuvo como objetivo general comprender las concepciones de seis maestros de un colegio de la ciudad de Barranquilla (Colombia) sobre la creatividad y su enseñanza y la manera como éstas se reflejan en su práctica pedagógica. Para ello se desarrolló una investigación de carácter cualitativo dentro de un diseño interpretativo-explicativo, que no sólo dio cuenta de las teorías implícitas que los maestros tenían sobre la creatividad y su enseñanza, sino que igualmente permitió comprender por qué se dan. Para ello, en primer lugar se establecieron y describieron las concepciones de un «Grupo de referencia¼ sobre la creatividad y su enseñanza; en segundo lugar se comprendieron las concepciones de los casos de estudio sobre el fenómeno de estudio; en tercer lugar se compararon las concepciones de los casos con las concepciones del Grupo de referencia, y finalmente se contrastó la práctica pedagógica de los casos (en su fase interactiva) con las concepciones que éstos tenían de la creatividad y su enseñanza. De las conclusiones de la investigación se destacan las siguientes: la evaluación de la creatividad se realiza a través de los productos concretos del estudiante; independiente del área de conocimiento se comparten concepciones generales sobre el fenómeno de la creatividad; no hay una enseñanza única para cada concepción de la creatividad; las concepciones sobre la creatividad y su enseñanza inciden en la forma como los sujetos desarrollan su práctica de aula y cada concepción de los casos tiene sus propias características que la hacen única.

This research aimed at understanding the general concepts on creativity and its teaching of six teachers from a school in the city of Barranquilla and how these concepts are reflected in their teaching practice. A qualitative research was developed within an interpretative- explanatory design was developed and it not only accounted for the implicit theories on creativity and its teaching on the part of the teachers, but it also allowed the understanding of the reason why they exist. As an initial step, the conceptions of a "reference group" were established and described. Secondly, the conceptions of the case study individuals concerning this subject were understood. Thirdly, the conceptions of the cases studied were compared with those of the "reference group" and finally, the teaching practice (in its interactive phase) of the cases was contrasted with the conceptions they had on creativity and its teaching. From the findings of this research we can conclude that: creativity is assessed through the concrete products of the students. General concepts on the creativity issue are shared independently of the area of knowledge; there is not a unique teaching for each conception of creativity; The conceptions on creativity and its teaching do have an influence on the way subjects develop their classroom teaching and each conception of the subjects has its own characteristics that make it unique.

Influencia de la gestación de bajo riesgo en la calidad de vida relacionada con la salud percibida por las gestantes / Influence of low-risk pregnancy on self-perceived health-related quality of life in pregnant women

Objetivos: Valorar la calidad de vida relacionada con la salud (CVRS) percibida por las gestantes de bajo riesgo en comparación con los valores poblacionales de referencia para mujeres del mismo grupo de edad; determinar si la gestación de bajo riesgo puede modificar la CVRS percibida; relacionar los «cambios fisiológicos de la gestación» con la pérdida de calidad de vida percibida; obtener valores de CVRS de referencia del cuestionario Medical Outcomes Study (MOS) 36-Item Short Form, o cuestionario SF-36, para gestantes del primer y tercer trimestres, dado que hasta la actualidad no hemos encontrado publicaciones españolas similares. Material y métodos: Encuesta transversal de prevalencia y comparación de 2 grupos independientes. Como instrumento de medición se utilizó el Medical Outcomes Study 36-Item Short Form (SF-36). Conclusiones: Las gestantes presentan una pérdida estadísticamente significativa de las dimensiones físicas cuando se comparan con los valores poblacionales de referencia para mujeres del mismo intervalo de edad; esta pérdida es más significativa al progresar la gestación, es decir, la progresión cronológica del embarazo normal puede alterar la percepción en la calidad de vida percibida por las gestantes, especialmente en el campo físico. No se observan alteraciones en las dimensiones relacionadas con la función emocional o social. Los síntomas propios de la gestación, como las náuseas y los vómitos, son capaces de alterar las funciones física y emocional

Objectives: The aims of the present study were four-fold: to assess health-related quality of life (QoL) perceived by women with low-risk pregnancies in comparison with reference population values for women in the same age group; to determine whether low risk pregnancy by itself can modify perceived QoL; to determine the association between the physiological changes of pregnancy and perceived loss of QoL; to obtain reference values for QoL from the questionnaire Medical Outcomes Study (MOS) 36-Item Short Form (SF-36) for expectant mothers in their first or third trimester, given that to date we have found no Spanish publications on the topic. Material and methods: We performed a cross-sectional survey of prevalence, with comparison of two independent groups. Measuring instrument: SF-36. Conclusions: The expectant mothers showed a statistically significant loss in physical dimensions in comparison with the reference population values for women in the same age group; this loss was more significant as the pregnancy progressed, that is, the chronological progression of a normal pregnancy is, by itself, able to alter the quality of life perceived by the pregnant women, especially in the physical domain. No changes in the dimensions related to social or emotional functions were observed. The typical symptoms of pregnancy such as nausea and vomiting are able to alter physical and emotional functions

Obesidad en pacientes adultos del municipio Sucre del estado Miranda / Obesity in adults in the municipality of Sucre state Miranda

Se evaluó el estado nutricional de 360 adultos que consultaron los ambulatorios "Don Pedro del Corral", "Araguaney" y las Clínicas Móviles, entre abril y junio de 2005, para el diagnóstico de obesidad, la cual se determinó según el índice de masa corporal sugerido por la Organización Mundial de la Salud (OMS) y se clasificó la obesidad en tipo I, tipo II y tipo III según la OMS. 28,88 por ciento de los pacientes femeninos que consultaron presentaron obesidad y 23,33 por ciento de los pacientes masculinos fueron obesos. Hubo obesidad de tipo I en un 65,79 por ciento, de tipo II en un 23,68 por ciento y de tipo III en un 10,52 por ciento. Los datos obtenidos fueron significativamente superiores a los datos publicados internacionalmente en Europa, Estados Unidos y el resto de Latinoamérica.

Manejo de la fertilización en el rendimiento, calidad del jugo y actividad de invertasas en caña de azúcar / Fertilization management for yield, juice quality, and invertase activity in sugarcane

El exceso de N en el tejido vegetal en la época de cosecha es la principal causa del bajo contenido de sacarosa en el jugo de caña de azúcar (Saccharum officinarum L.). El contenido de N es el producto del balance entre cantidad total de sacarosa sintetizada y cantidad hidrolizada por la actividad de invertasas ácida y neutra. El objetivo del trabajo fue determinar la relación entre la calidad del jugo y de caña de azúcar (Brix puerza, concentraciones de sacarosa y azúcares reductores) y la actividad de las invertasas ácidas y neutras, en plantas sometidas a diferentes manejos de fertlización. El experiemnto se realizó en planataciones de caña establecidas con las variedades Méx 69-290 (soca) en un Vertisol y Méx 68-P-23 (resoca 3) en n Inceptisol, en Tabasco, México. Los tratamientos fueron combinciones de 160 y 200kg.ha de N con 67 y 202 kg.ha de dos 1 o 3 meses después del rebrote. Todos los tratamientos recibieron 35kg.ha de P, excepto un tratamiento testigo sin fertilizante. Los tratamientos se acomodaron en eun arreglo de bloques al azar con cuatro repeticiones. La caña fue cosechada a los 10,5 y 11,5 meses de edad en el Vertisol e Inceptisol, respectivamente. El tratamiento 160-35-67 N-P-K (kg.ha), aplicado como mezcla de sulfato de amonio, superfosfato triple y KCI, enterrado tres meses despues del rebrote, produjo rendimientos mayores a los del testigo en ambos suelos (130,7 y 113,6ton.ha). La calidad del jugo de caña no fue afectada por las dosis de fertilización estudiadas, ni la actividad de la invertasas fue modificada por los diferentes manejos

Cierre percutáneo del conducto arterioso con espiral de gianturco en lactante de 3.2 Kg de peso / Percutaneous closure of patent ductus arterious in an infant with a gianturco coil

Se reporta un lactante de 38 días de vida, con 3.2 kg de peso y portador de síndrome de Down, soplo cardiaco y taquipnea. En el ecocardiograma Doppler color se encuentran persistencia del conducto arterioso e hipertensión arterial pulmonar. Se realizó cateterismo cardiaco que confirmó conducto arterioso permeable del tipo A-2 de 1.5 mm en su diámetro más angosto, con Qp/Qs de 1.8 e hipertensión arterial pulmonar. Se colocó espiral metálica de 5 cm de largo x 5 mm de diámetro por vía anterógrada a través de vena femoral, con oclusión completa del conducto y desaparición de hiperflujo e hipertensión arterial pulmonar. Concluimos que, en lactantes pequeños como en este caso, el uso de espiral metálica es una buena alternativa para el cierre del ducto arterioso

Correción del desequilibrio hidroelectrolítico, en recién nacidos con rehidratación por vía oral / Correction with oral rehydration of the hydroelectrolytic imbalance in the newborn infant

En un estudio previo se evaluó la eficacia de la solución por vía oral propuesta por la OMS para corregir el desequilibrio electrolítico y ácido-base, en 250 niños deshidratados por gastroenteritis. En el presente trabajo se analizan los resultados en los 22 recién nacidos de ese grupo. La edad promedio fue de 16 días; la evolución de la diarrea de 3,9 días y la deshidratación de 6.7%. La rehidratación se logró en promedio de seis horas. La corrección del desequilibrio hidroelectrolítico y ácido-base fue satisfactoria en todos los casos y no se observaron complicaciones durante el procedimiento