RESUMO
The safety and immunological activity of P. aeruginosa vaccine were experimentally evaluated. The vaccine was prepared on the basis of the antigens of P. aeruginosa extracellular slime which was accumulated in medium K-4, obtained with the use of original technology. The immunization of animals with P. aeruginosa vaccine induced the synthesis of antibodies. The introduction of the vaccine in 2 or 3 injections resulted in a high level of antibody formation, differing with the use of various strains. Hyperimmune sera, obtained by the multiple immunization of rabbits with P. aeruginosa vaccine, ensured high protection of mice from P. aeruginosa infection. The vaccine proved to be safe when evaluated in experiments of acute and chronic toxicity, made on laboratory animals.
Assuntos
Antígenos de Bactérias/imunologia , Vacinas Bacterianas/administração & dosagem , Infecções por Pseudomonas/prevenção & controle , Pseudomonas aeruginosa/imunologia , Vacinação , Animais , Anticorpos Antibacterianos/biossíntese , Especificidade de Anticorpos , Antígenos de Bactérias/toxicidade , Vacinas Bacterianas/isolamento & purificação , Vacinas Bacterianas/toxicidade , Meios de Cultura , Hidroxilamina , Injeções Subcutâneas , Camundongos , Infecções por Pseudomonas/imunologia , Pseudomonas aeruginosa/crescimento & desenvolvimento , CoelhosRESUMO
The possibility of using experimental culture medium K-4 prepared on the basis of casein hydrolysate peptides with the isoelectric point 4.1 for obtaining antigens from P. aeruginosa strains was evaluated. Two antigenic fractions were isolated from the culture fluid containing extracellular slime. The study of the toxicity of the antigenic preparations revealed that one of these fractions had low toxicity for mice (the second antigenic fraction was highly toxic). The former P. aeruginosa antigenic fraction was used for obtaining pyocyanic vaccine. One vaccination dose of this vaccine contained 0.2 mg of the antigen adsorbed on aluminum hydroxide. Pyocyanic vaccine ensured the active protection of mice challenged with P. aeruginosa homologous and heterologous strains.
Assuntos
Antígenos de Bactérias/administração & dosagem , Meios de Cultura/química , Infecções por Pseudomonas/prevenção & controle , Pseudomonas aeruginosa/imunologia , Vacinação , Animais , Antígenos de Bactérias/isolamento & purificação , Antígenos de Bactérias/toxicidade , Centrifugação , Precipitação Química , Diálise , Modelos Animais de Doenças , Hidroxilamina , Injeções Intraperitoneais , Dose Letal Mediana , Camundongos , Pseudomonas aeruginosa/crescimento & desenvolvimento , Vacinas de Subunidades Antigênicas/administração & dosagem , Vacinas de Subunidades Antigênicas/isolamento & purificação , Vacinas de Subunidades Antigênicas/toxicidadeRESUMO
The test for the evaluation of the toxicity of different types of pertussis preparations as manifested by their in vitro influence on mouse thymic cells (T test) has been finally worked out. The use of the T test has made it possible to reveal the nonstandard character of the production lots of adsorbed diphtheria-pertussis-tetanus vaccines, both whole-cell vaccine and Japanese acellular vaccine. The degree of the in vitro damaging action of pertussis preparations on mouse thymic cells greatly depends on the residual content of Bordetella pertussis nontoxoidized toxin which, in contrast to B. pertussis lipopolysaccharide and filamentous hemagglutinin, produces pronounced cytotoxic action on mouse thymic cells.
Assuntos
Vacina contra Coqueluche/toxicidade , Timo/efeitos dos fármacos , Animais , Peso Corporal/efeitos dos fármacos , Células Cultivadas/efeitos dos fármacos , Vacina contra Difteria, Tétano e Coqueluche/toxicidade , Relação Dose-Resposta a Droga , Dose Letal Mediana , Camundongos , Camundongos Endogâmicos CBA , Timo/citologia , Fatores de TempoRESUMO
The influence exerted by histamine on the capacity of T-lymphocytes for spontaneous rosette formation in intact animals and in animals immunized with adsorbed DPT vaccine and its components has been studied. Histamine at a concentration of 10(-3) M has been found to produce an inhibitory effect on the capacity of lymphocytes in the blood and spleen of guinea pigs immunized with adsorbed DPT vaccine and its components for spontaneous rosette formation. This effect of histamine has proved to be even more pronounced after the immunization of the animals with adsorbed DPT vaccine and Bordetella pertussis suspension, which is probably due to their sensitizing action.
Assuntos
Toxoide Diftérico , Histamina/imunologia , Vacina contra Coqueluche , Formação de Roseta , Linfócitos T/imunologia , Toxoide Tetânico , Animais , Vacina contra Difteria, Tétano e Coqueluche , Combinação de Medicamentos , CobaiasRESUMO
The results obtained in the study of the influence of histamine on the capacity of T-lymphocytes of guinea pigs immunized with DPT-vaccine and its components for spontaneous rosette formation are presented. Histamine at a concentration of 10(-3) M has been found to inhibit the capacity of blood and splenic lymphocytes of guinea pigs immunized with adsorbed DPT vaccine for spontaneous rosette formation. The inhibitory effect is more pronounced after the immunization of the animals with adsorbed DPT vaccine and Bordetella pertussis suspension.