A Targeted Survey for Scrapie in Jos Plateau State, Nigeria.

Scrapie, a disease of sheep and goats with a progressive course and fatal outcome, has not been identified in Nigeria. Anecdotal scrapie reports by livestock workers abound. Livestock diseases like scrapie form huddles in livestock economics of countries. For 8 months we surveyed for scrapie targeting emergency/casualty slaughter sheep and goats in Jos, Nigeria. We clinically examined 510 sheep and 608 goats of local breeds, aged from 12 months to 5 years. In total 31 (5.10%) goats and no sheep were clinically suspicious for scrapie. Caudal brainstem tissues of suspect animals collected postmortem were analyzed for the disease specific form of the prion protein, PrP(Sc), using Bio-Rad's TeSeE ELISA rapid test kit. No sample was positive for scrapie. Fluorescent antibody test for rabies and H&E staining on samples were carried out for differential diagnosis. These showed no pathological lesions indicative for neurological disease. While our findings do not exclude the presence of scrapie in Jos, we demonstrate that targeted sampling of small ruminants for neuroinfectious disease is feasible in developing countries, pointing to the possibility of implementing such a monitoring scheme in Nigeria to prevent economic losses in small ruminant livestock as scrapie caveats from endemic countries have shown.

A pilot study for targeted surveillance of bovine spongiform encephalopathy in Nigeria.

Bovine spongiform encephalopathy (BSE), popularly known as 'mad cow disease', led to an epidemic in Europe that peaked in the mid-1990s. Its impact on developing countries, such as Nigeria, has not been fully established as information on livestock and surveillance has eluded those in charge of this task. The BSE risk to Nigeria's cattle population currently remains undetermined, which has resulted in international trade restrictions on commodities from the cattle population. This is mainly because of a lack of updated BSE risk assessments and disease surveillance data. To evaluate the feasibility of BSE surveillance in Nigeria, we carried out a pilot study targeting cattle that were presented for emergency or casualty slaughter. In total, 1551 cattle of local breeds, aged 24 months and above were clinically examined. Ataxia, recumbency and other neurological signs were topmost on our list of criteria. A total of 96 cattle, which correspond to 6.2%, presented clinical signs that supported a suspect of BSE. The caudal brainstem tissues of these animals were collected post-mortem and analysed for the disease-specific form of the prion protein using a rapid test approved by the International Animal Health Organization (OIE). None of the samples were positive for BSE. Although our findings do not exclude the presence of BSE in Nigeria, they do demonstrate that targeted sampling of clinically suspected cases of BSE is feasible in developing countries. In addition, these findings point to the possibility of implementing clinical monitoring schemes for BSE and potentially other diseases with grave economic and public health consequences.

Studies on Prevalence and Risk Factors for Hepatitis B Surface Antigen among Secondary School Students in North-Central; Nigeria

Hepatitis B virus (HBV) Infection is one of the major global public health problems; with its attendant risks especially among the young adults. This study was therefore carried out to assess the sero-prevalence of hepatitis B surface antigen (HBsAg) and associated risk factors among students of a secondary school in Jagindi Tasha; Kaduna State; Nigeria. One hundred and ninety (190) apparently healthy students were screened and sera samples obtained were separated and analysed for HBsAg using a commercially available Enzyme Linked Immunosorbent Assay (ELISA)-based kit (Dialab). Information was obtained for risk factors using structured questionnaire. Out of the 190 samples screened; 35 (18.4) were sero-positive. Subjects aged 13-15 years recorded 6.8 positivity (x2 = 1.084; p0.05) and male subjects had 25.5 positivity compared to 10.9 positivity for females (x2 = 6.768; p0.05). Risk factors such as blood transfusion was 32.0 among male subjects compared to 30.0 in females (x2

Seroprevalence of Hepatitis B Surface Antigen (HBsAg) among Children of Primary School Age in a Community; North-Central; Nigeria

Globally; Hepatitis B Virus has been identified as one of the most common infectious diseases and a major public health problem.This study was therefore carried out to assess the prevalence of Hepatitis B virus infection among primary school children attending LGE primary school; Sabon Pegi; Kuru; Nigeria. Three hundred and sixty (360) blood samples were collected from the pupils and sera separated and analyzed for HBsAg using one step Hepatitis B surface antigen test strip. Of the 360 samples screened; 35 (9.7) were sero-positive. Pupils within the age 7-9 years had the highest prevalence of 3.9. Male subjects recorded a prevalence of 6.1compared to 3.6for females. Risk factors such as blood transfusion recorded 1.6. Furthermore; family history of HBV infection accounted for 3.6; while male subjects that had traditional method of circumcision recorded a high prevalence of 3.3. Unfortunately; the prevalence of this ravaging infectious agent appears to be high amongst the subjects studied. It is strongly suggested that public awareness be accorded urgent attention while socioeconomic development in these areas be given priority as a measure to preventing further spread of this virus amongst children; particularly the school age group