In vivo assessment of changes to canine airway smooth muscle following bronchial thermoplasty with OR-OCT.

The inability to assess and measure changes to the airway smooth muscle (ASM) in vivo is a major challenge to evaluating asthma and its clinical outcomes. Bronchial thermoplasty (BT) is a therapy for asthma that aims to reduce the severity of excessive bronchoconstriction by ablating ASM. Although multiple long-term clinical studies of BT have produced encouraging results, the outcomes of BT treatment in practice have been variable, and questions remain regarding the selection of patients. Previously, we have demonstrated an imaging platform called orientation-resolved optical coherence tomography that can assess ASM endoscopically using an imaging catheter compatible with bronchoscopy. In this work, we present results obtained from a longitudinal BT study performed using a canine model (n = 8) and with the goal of investigating the use of orientation-resolved optical coherence tomography (OR-OCT) for measuring the effects of BT on ASM. We demonstrate that we are capable of accurately assessing ASM both before and in the weeks following the BT procedure using blinded matching to histological samples stained with Masson's trichrome (P < 0.0001, r2 = 0.79). Analysis of volumetric ASM distributions revealed significant decreases in ASM in treated airways (average cross-sectional ASM area: 0.245 ± 0.145 mm2 pre-BT and 0.166 ± 0.112 mm2 6 wk following BT). These results demonstrate that OR-OCT can provide clinicians with the feedback necessary to better evaluate ASM and its response to BT, and may potentially play an important role in phenotyping asthma and predicting which patients are most likely to respond to BT treatment.NEW & NOTEWORTHY The inability to assess ASM in vivo is a significant hurdle in advancing our understanding of airway diseases such as asthma, as well as evaluating potential treatments and therapies. In this study, we demonstrate that endoscopic OR-OCT can be used to accurately measure changes to ASM structure following BT. Our results demonstrate how this technology could occupy an important role in asthma treatments targeting ASM.

Numerical investigation of bubble-induced Marangoni convection.

The liquid motion induced by surface tension variation, termed the thermocapillary or Marangoni effect, and its contribution to boiling heat transfer has long been a very controversial issue. In the past this convection was not the subject of much attention because, under terrestrial conditions, it is superimposed by the strong buoyancy convection, which makes it difficult to obtain quantitative experimental results. The scenario under consideration in this paper may be applicable to the analysis of boiling heat transfer, specifically the bubble waiting period and, possibly, the bubble growth period. To elucidate the influence of Marangoni convection on local heat transfer, this work numerically investigates the presence of a hemispherical bubble of constant radius, R(b)= 1.0 mm, situated on a heated wall immersed in a liquid silicone oil (Pr= 82.5) layer of constant depth H= 5.0 mm. A comprehensive description of the flow driven by surface tension gradients along the liquid-vapor interface required the solution of the nonlinear equations of free-surface hydrodynamics. For this problem, the procedure involved solution of the coupled equations of fluid mechanics and heat transfer using the finite-difference numerical technique. Simulations were carried out under zero-gravity conditions for temperatures of 50, 40, 30, 20, 10, and 1 K, corresponding to Marangoni numbers of 915, 732, 550, 366, 183, and 18.3, respectively. The predicted thermal and flow fields have been used to describe the enhancement of the heat transfer as a result of thermocapillary convection around a stationary bubble maintained on a heated surface. It was found that the heat transfer enhancement, as quantified by both the radius of enhancement and the ratio of Marangoni heat transfer to that of pure molecular diffusion, increases asymptotically with increasing Marangoni number. For the range of Marangoni numbers tested, a 1.18-fold improvement in the heat transfer was predicted within the region of R(b)< or = r< or = 7R(b).