Résultats du traitement des fractures de la diaphyse humérale au CHU d'Owendo

Introduction. Le traitement des fractures de la diaphyse humérale bénéficie d'un arsenal thérapeutique et varié. À Libreville, il n'est pas consensuel du fait des nombreuses écoles dont sont issus les praticiens. Le but de notre travail était d'évaluer les résultats du traitement de ces fractures dans notre service dans ce contexte particulier. Matériels et méthodes. Il s'agit d'une étude rétrospective sur un an allant du 1er mars 2017 au 1er mars 2018. Il a porté sur 33 patients traités dans le service et régulièrement suivis jusqu'à consolidation. Pour chaque patient, les paramètres analysés ont été : l'âge et le sexe, le mécanisme et les circonstances de survenue, le membre concerné et les délais de prise en charge, le type anatomopathologique, les complications immédiates et lésions associés, les méthodes thérapeutiques utilisées et les résultats anatomiques et fonctionnels. Résultats. Il y avait 20 hommes et 13 femmes ; leur âge moyen était de 33 ans. Dans 27 cas (83%), il s'agissait d'accidents de la voie publique. Treize patients (39 %) ont bénéficié d'un traitement orthopédique par plâtre et 20 patients (61 %) ont bénéficié d'un traitement chirurgical. 10 patients (84%) ayant eu un traitement orthopédique ont des bons / très bons résultats. 16 patients (80%) des patients ayant bénéficié d'un traitement chirurgical ont eu des bons et très bons résultats. Conclusion. Nos résultats (anatomiques ou fonctionnels) sont superposables entre le traitement chirurgical et le traitement orthopédique

Envelope sequence variability and serologic characterization of HIV type 1 group O isolates from equatorial guinea.

Four sera from Equatorial Guinea (EG) suspected to contain antibody against HIV-1 group O-related viruses were identified on the basis of unusual and differential serologic reactivity in selected commercial assays and Western blot. Degenerate primers, designed from HIV-1 group O published sequences, were used to PCR amplify envelope (env) gene sequences from the suspect EG sera. A complete envelope gene sequence from each serum was determined from the overlapping env gene fragments. Analysis (PHYLIP package of programs) of Env amino acid sequences (translated from nucleotide sequences) indicated that the amino acid sequences obtained from EG sera clustered more closely with HIV Env sequences of group O compared to group M. The amino acid sequences at the octameric tip of the V3 loop were either RIGPLAWY (one isolate), RIGPMAWY (two isolates), or GLGPLAVY (one isolate). The V3 tip tetrameric sequence GPLA is represented only once in the 1995 HIV (Los Alamos) database, but was present in two of our group O-related EG samples. The gp41 immunodominant regions (IDR) protein sequences were identical for sequences from three of the sera, RLLALETLIQNQQLLNLWGCKGR(K)L(I)VCYTSVK(T)W, whereas sequence from the fourth serum contained three changes as noted in parentheses. IDR sequences derived from EG sera were unique compared to those reported for other HIV-1 group O isolate ANT70, VAU, or MVP5180. Antibody in each EG serum directed against the IDR could be detected using synthetic peptides comprising sequences from the ANT70 or MVP5180 IDRs, but were most reactive against the sequences derived from the samples themselves. Little or no serologic reactivity was detected when EG sera were reacted against peptides comprising the IDR of HIV-1 group M (subtype B consensus) or HIV-2 (consensus).

PIP: The genetic variation and epidemiology of HIV-1 group O isolates are of considerable importance to the design of HIV-1 diagnostic and screening assays, especially since current serologic and genetic methods to detect HIV-1 have been developed mainly on the basis of sequences from isolates belonging to HIV-1 group M. The HIV envelope protein, especially the gp41 immunodominant region, plays a major antigenic role in the detection of HIV infection and for discriminating HIV-1 from HIV-2 antibody. This paper reports upon genetic variation and the serologic characterization of env sequences from 4 people living in Equatorial Guinea (EG) who were infected with HIV-1 group O. Selected commercial assays and Western blot were first used to identify the sera, then degenerate primers, designed from HIV-1 group O published sequences, were used to PCR amplify envelope (env) gene sequences. A complete envelope gene sequence from each serum was determined from the overlapping env gene fragments. The env amino acid sequence analysis found the EG sera sequences to be clustered more closely with the HIV env sequences of group O rather than to group M. The amino acid sequences at the octameric tip of the V3 loop were either RIGPLAWY, RIGPMAWY, or GLGPLAVY. Although the V3 tip tetrameric sequence GPLA is represented only once in the 1995 HIV database, it was present in 2 of the group O-related EG samples. The gp41 immunodominant regions (IDR) protein sequences were identical for sequences from 3 of the sera. IDR sequences derived from the EG sera were unique compared to those reported for other HIV-1 group O isolates ANT70, VAU, or MVP5180. Other findings are discussed in detail.