Review: Strategies for enteric methane mitigation in cattle fed tropical forages.

Methane (CH4) is a greenhouse gas (GHG) produced and released by eructation to the atmosphere in large volumes by ruminants. Enteric CH4 contributes significantly to global GHG emissions arising from animal agriculture. It has been contended that tropical grasses produce higher emissions of enteric CH4 than temperate grasses, when they are fed to ruminants. A number of experiments have been performed in respiration chambers and head-boxes to assess the enteric CH4 mitigation potential of foliage and pods of tropical plants, as well as nitrates (NO3-) and vegetable oils in practical rations for cattle. On the basis of individual determinations of enteric CH4 carried out in respiration chambers, the average CH4 yield for cattle fed low-quality tropical grasses (>70% ration DM) was 17.0 g CH4/kg DM intake. Results showed that when foliage and ground pods of tropical trees and shrubs were incorporated in cattle rations, methane yield (g CH4/kg DM intake) was decreased by 10% to 25%, depending on plant species and level of intake of the ration. Incorporation of nitrates and vegetable oils in the ration decreased enteric CH4 yield by ∼6% to ∼20%, respectively. Condensed tannins, saponins and starch contained in foliages, pods and seeds of tropical trees and shrubs, as well as nitrates and vegetable oils, can be fed to cattle to mitigate enteric CH4 emissions under smallholder conditions. Strategies for enteric CH4 mitigation in cattle grazing low-quality tropical forages can effectively increase productivity while decreasing enteric CH4 emissions in absolute terms and per unit of product (e.g. meat, milk), thus reducing the contribution of ruminants to GHG emissions and therefore to climate change.

PRIMER REPORTE DE Cryptosporidium parvum EN TERNEROS HOLSTEIN (Bos taurus) DE MANIZALES, CALDAS, COLOMBIA / FIRST REPORT OF Cryptosporidium parvum IN HOLSTEIN CALVES (Bos taurus) FROM MANIZALES, CALDAS, COLOMBIA

El objetivo de este estudio fue identificar especies o genotipos del protozoario parásito Cryptosporidium presentes en heces colectadas de terneros Holstein del municipio de Manizales, Departamento de Caldas, Colombia. El ADN fue extraído a 80 muestras de materia fecal, de las cuales 11 fueron diagnosticadas positivas para Cryptosporidium spp., mediante reacción en cadena de la polimerasa (PCR). El análisis PCR-RFLP del locus 18S ADNr, identificó la presencia de Cryptosporidium parvum en todas las muestras positivas analizadas. Este hallazgo sugiere que el ganado puede ser una fuente potencial de infección por Cryptosporidium en humanos y se constituye en el primer reporte publicado de C. parvum en bovinos de Manizales, Caldas.

The objective of this study was to identify species or genotypes of Cryptosporidium parasite present in feces collected from Holstein calves in Manizales city, Caldas Department, Colombia. DNA was extracted from 80 fecal samples, which 11 were diagnosed positive for Cryptosporidium spp., by the Polymerase Chain Reaction method. PCR-RFLP analysis of 18S rDNA locus identified the presence of Cryptosporidium parvum in all samples tested positive. This finding suggests that cattle may be a potential source of human infection by Cryptosporidium, and it becomes the first published report of C. parvum in cattle in Manizales, Caldas.

Disordered eating and suicidal intent: the role of thin ideal internalisation, shame and family criticism.

OBJECTIVE: We explored the effect of thin ideal internalisation, shame proneness and family criticism on disordered eating and suicidal intent in female Mexican adolescents. METHOD: We studied a probabilistic sample of 2537 high school students in central Mexico, stratified by marginalisation status and migratory intensity. We used a generalised logistic regression model to estimate the odds of disordered eating and suicidal intent across scores for three predictors: Internalisation of the thin ideal, shame and family criticism. RESULTS: Disordered eating was reported by 4.2% (95% CI = 0.9-7.5%) and suicidal intent by 13.2% (95% CI = 12.0-14.4%) of girls. The unadjusted odds ratios of any disordered eating for thin ideal internalisation, shame proneness and familial criticism were 1.2, 1.1 and 3.2, respectively. The positive association between thin ideal internalisation and disordered eating remained even after controlling for shame proneness and familial criticism. The association of these variables with suicidal intent was weaker. DISCUSSION: Results support stronger effects for disordered eating than suicidal intent across the three unadjusted predictors. It also highlights the presence of the relationship of criticism and disordered eating in female adolescents from low and middle socio-economic backgrounds.

Medical ethnobotany of the Teribes of Bocas del Toro, Panama.

Ethnomedical uses of 108 medicinal plant species, belonging to 52 families, 89 genera, used by the Teribe Amerindians of Bocas del Toro Province in Panama, along with their socio-cultural practices are reported here. The methods of administration of the herbal remedies, the plant parts used, their families and local names are also documented. The recorded medicinal plants were used mainly for fever, various type of pain and inflammation. The potential value of 26 plants and their traditional uses was elucidated through literature search.

Caracterización de linfocitos T gingivales en pacientes con periodontitis agresiva / Characterizarion of gingival T lymphocytes in patients with aggressive periodontitis

En este estudio se investigó la localización y distribución de os linfocitos T en el tejido gingival afectado de pacientes con periodontits agresiva (PA) y gingivitis inducida por placa bacteriana (GIPB). Las biopsias se obtuvieron de 20 pacientes entre 18 y 41 años de edad y fueron procesadas para estudio histpatológico e inmunohistoquímico. Los linfocitos T gingivales (CD3+), las células T ayudantes (CD4+) y las células T supresoras-citotóxicas (CD8+) fueron identificadas usando anticuerpos monoclonales y la técnica de inmunoperoxidasa. El infiltrado inflamatorio en GIPB fue escaso y se localizó principalmente en la lámina propia debajo del epitelio sulcular y de unión y estuvo dominado por linfocitos T, mientras que en las biopsias de periodontitis agresiva se localizó primordialmente en el tejido conjuntivo profundo, debajo del epitelio de la bolsa y estaba compuesto por linfocitos y células plasmáticas. El porcentaje de células CD3+ disminuyó en PA, al compararlo con GIPB, como consecuencia de una reducción en las células CD4+, especialmente. Estos hallazgos sugieren un papel inmunorregulador de las células T en la patogenia de las enfermedades periodontales.

Non-contrast computed tomography-guided intra-articular corticosteroid injections of severe bilateral hip arthritis in a patient with ankylosing spondylitis.

Progressively destructive hip arthritis is a common complication of patients with ankylosing spondylitis. Palliative intra-articular corticosteroid hip injections are helpful in younger patients who wish to delay joint replacement surgery. Non-contrast computed tomography (CT)-guided intra-articular procedures can potentially minimize radiation exposure compared to fluoroscopy-guided techniques. We describe a CT-guided technique for intra-articular corticosteroid hip injections for severe bilateral hip arthritis which avoids the use of radiologic contrast by documenting the presence of a pre-injection air bubble underneath the fibrous capsule. Significant reduction in symptoms within 48 hours and at 6 weeks post-injection confirmed the successful outcome of this technique.

Catalyzed Reformatsky reactions with ethyl bromofluoroacetate for the synthesis of alpha-fluoro-beta-hydroxy acids.

The presence of catalytic amounts of CeCl(3) improves yields and simplifies procedure in the Reformatsky reactions of ethyl bromofluoroacetate with aldehydes and ketones to generate diastereomeric mixtures of alpha-fluoro-beta-hydroxy esters, some of which can be separated by crystallization or column flash chromatography. Diastereomerically pure alpha-fluoro-beta-hydroxy acids are obtained by mild alkaline hydrolysis of the resolved alpha-fluoro-beta-hydroxy esters. Detailed NMR data of new alpha-fluoro-beta-hydroxy esters and alpha-fluoro-beta-hydroxy acids are also presented.

Diffusion- and perfusion-weighted MR imaging of dural sinus thrombosis.

A patient with dural sinus thrombosis had progressively worsening symptoms and signs that resolved after intradural thrombolysis. Intradural sinus pressures were 54 mm Hg. Echo-planar MR imaging revealed complex abnormalities of diffusion and widespread delay in mean transit time that improved immediately after thrombolysis. This case suggests that diffusion- and perfusion-weighted imaging can provide valuable information noninvasively to help triage patients with dural sinus thrombosis between conservative and aggressive management.

Which monotherapy for hypertension

The comparative blood pressure lowering and hemodynamic effects of four types of antihypertensive drugs, i.e.indapamide (diuretic), atenalol (betablocker), verapamil (calcium antagonist), and captopril (converting enzyme inhibitor), were evaluated among 30 middle aged men with mild to moderate essential hypertension in a double-blind randomized multiple crossover study. Blood pressure reduction were comparable regardless of hypotensives used. However atenolol 100 mg once daily also produced significant reduction in exercise-induced rate-pressure product and improved LV compliance echocardiogram indices within 2 weeks. Antihypertensive therapy is lifelong. Thus a daily single dose drug is advantageous for better patient acceptability and compliance during longterm antihypertensive therapy.(Auth)

Aspectos que afectan el análisis de contenido de ADN por citometría de flujo / Factors that affect DNA content analysis by flow cytometry

DNA ploidy and cell cycle analysis by flow cytometry is used to obtain additional information about the diagnosis and prognosis of different types of cancer. However, there are several disagreements among authors about the tissue source (fresh-frozen or paraffin embedded), cellular dissociation methods (mechanical, enzymatic or other), use of different dyes, lasers, analysis software with different mathematical models and interpretation of results. A discussion about the different aspects that affect the study of DNA ploidy and cell cycle and a consensus in publications is mandatory. A strict control of analysis processes and data interpretation is also necessary

The predictive value of lymphoscintigraphy for nodal metastases of cutaneous melanoma.

Lymphoscintigraphy is used to identify ambiguous drainage patterns in cutaneous melanoma of the head, neck and trunk. This study evaluated the efficacy and reliability of lymphoscintigraphy to identify the drainage nodes in 51 patients undergoing both elective and therapeutic lymphadenectomy over a seven-year period. All 13 patients who had lymph node metastases during this follow-up period had the metastatic disease in the very lymph node basins identified by lymphoscintigraphy. Most significantly, none of the 51 patients had metastatic disease in lymphatic basins that were not previously identified by lymphoscintigraphy.

Low prevalences of HIV infection and sexually transmitted disease among female commercial sex workers in Mexico City.

OBJECTIVES: This study tried to determine human immunodeficiency virus (HIV)/sexually transmitted disease (STD) prevalences among female commercial sex workers in Mexico City. METHODS: A sampling frame was constructed that included bars, massage parlors, and street corners. RESULTS: Prevalences for Treponema pallidum, herpes simplex virus type 2, HIV, Neisseria gonorrhoeae, and Chlamydia trachomatis were 6.4%, 65%, 0.6%, 3.7%, and 11.1%, respectively. A significant association was found between higher STD frequencies and working at street sites. CONCLUSIONS: Most STD frequencies were lower in comparison with rates found for female sex workers in other countries. However, preventive programs against STD/ HIV are needed in this population.

Subtalar arthrorisis and associated procedures.

Not all flatfeet require surgical correction. Through a careful history obtained from the child's parents, useful clues may be gathered pertaining to the disability arising from the flatfoot. There are three distinct classifications of flexible pes pronatus that require specific procedures for correction of the primary deformity. The STA-Peg, alone or in combination with other medial arch procedures, is used for correction of hindfoot pronation in the coronal planar dominant foot. The postoperative disability associated with the STA-Peg is relatively minimal, and complications are usually due to improper insertion of the implant.

Sagittal synostosis.

Sagittal synostosis comprises a spectrum of deformities caused by premature fusion of the sagittal suture. The treatment of sagittal synostosis is dependent on the age of the patient and the characteristics of the presenting deformity. Immediate correction of the scaphocephalic deformity is the goal of operative treatment. The operative procedures proposed involve whole-vault cranial reshaping, active shortening of the anteroposterior axis, and widening of the mediolateral dimension of the skull leading to a more normal, "rounded" skull shape.

Dual-angled CT-guided biopsy.

A new computed tomographic (CT)-guided biopsy technique is described which employs angling both the CT-gantry and the patient to access lesions considered unapproachable using conventional CT techniques. Angling the gantry and the patient, or dual angulation, was applied in five patients with masses located in the pelvis and retroperitoneum that were not easily accessible using gantry angling or patient tilting alone. In each case, the needle tip was demonstrated in the lesion and diagnostic tissue was retrieved. No complications were recorded. A dual-angled approach defines a safe path to a mass and allows obtaining a CT image in the plane of the biopsy needle.

Estudio comparativo entre la biocompatibilidad histológica del Membraflex y el Gore-Tex como materiales para regeneración tisular guiada: estudio piloto / Comparative study between the histological biocompatibility of Membraflex and Gore-Tex as materials for guided tissue regeneration

El propósito de este estudio fue evaluar la respuesta histológica de dos materiales que pueden ser usados para regeneració tisular guiada usando un modelo subcutáneo en el abdomen de un primate no humano. Los materiales estudiados fueron dos membranas de politetrafluoretileno (PTFE): Gore-Tex (W.L. Gore Association, Inc., Flagstaff, Arizona, USA) y Membraflex (Laboratorio de Investigación, Productos Orales Ltda, Santa Fe de Bogotá, D.C., Colombia). Se colocaron 4 implantes en el primate bajo anestesia disociativa a las 5a. y 8a semanas, luego del procedimiento quirúrgico, se hicieron análisis histológicos de los tejidos que rodeaban las membranas y del tipo y cantidad del infiltrado celular alrededor de éstas. Se encontró un infiltrado de tipo crónico predominado por linfocitos, el cual disminuyó en la 8a. semana. Hubo una tendencia al encapsulamiento de las membranas mediante tejido conectivo fibroso denso. Se concluyó que el infiltrado inflamatorio producido por los dos tipos de membranas, al ser colocados subcutáneamente en un primate, es similar en calidad y cantidad a las 5 y 8 semanas postoperatorias

[The inequality of health services for the uninsured population in Mexico]. / Inequidad de los servicios de salud a población abierta en México.

In order to assess the coverage of health services for non-insured population receiving health care from the Ministry of Health (SSA), a cross-sectional study was developed. With official statistics of SSA and data from the XI General Census of Population and Housing (1990), ratios of coverage and mortality rates in hospitals were assessed. The data were processed through simple analysis. In addition, states were stratified by groups according to poverty indicators obtained through a factorial analysis with the method of multiple components. The results showed that a polarized health economy for the non-insured population in Mexico is the cause of complex difficulties in the health care provision. The low financial resources affect the distribution of health care services in the states. Seventy-six per cent of the non-insured population is concentrated in those states with the highest levels of poverty, with a potential coverage of only 34.2 per cent and just 63.4 per cent of potential population use. The coverage of health care services in the states with lower poverty indicators is 1.9 times that of the states with higher poverty indicators. It was also observed that hospital mortality rate among children less than one year old was 1.7 times higher in poor regions. In general, the health services coverage is low. Therefore, it is necessary to expand the access to health care and improve the quality of services as well as life conditions of the population in the states with the highest poverty indicators. If this occurs, it will be possible to close the gap in order to build a better National Health System.

[Trends in health services research in Mexico, 1984-1991]. / Tendencias de investigación en servicios de salud en México, 1984-1991.

Health services research is a basic tool for the development of the National Health System. This paper presents the results of a national survey on research projects carried out in Mexican health institutions, as well as in universities and research centers, from 1984 to 1991.

Barrett's esophagus in children. Diagnosis and management.

OBJECTIVE: To determine the local prevalence and optimal therapy for children with Barrett's esophagus (BE), the authors studied children with esophageal strictures or gastroesophageal reflux (GER), or both, to diagnose BE and to follow after therapy. SUMMARY BACKGROUND DATA: Barrett's esophagus is seldom reported in children and therapeutic recommendations are unclear. Barrett's esophagus usually develops during the mucosal reparative process after acid-reflux injury to the esophageal mucosa. Risk factors for BE include conditions that are associated with GER such as mental retardation, esophageal stricture, esophageal atresia, and reversed gastric tube esophagoplasty. Barrett's syndrome increases the risk of esophageal adenocarcinoma by 30 to 40 times. METHODS: All children with the risk factors had repeated esophagoscopy and multiple mucosal biopsies before and after therapy. RESULTS: Eleven children have been documented with BE. The initial diagnoses were: GER, 5; esophageal atresia, 4; nasogastric intubation, 1; lye ingestion, 1. A gastric tube esophagoplasty had been performed in three patients with BE in the esophagus proximal to the anastomosis. Three children with mid-esophageal strictures and long segments of BE had total resection with colic interposition. An additional two patients with tight stricture were treated with colic-patch esophagoplasty without resection. The final three patients were treated with fundoplication alone. CONCLUSIONS: Barrett's esophagus can be caused by acid from gastric tubes but responds to H2 blockers and diet. Resection eliminates BE; esophagoplasty only controls the stricture and must be accompanied by fundoplication. Barrett's esophagus persists in patients with fundoplication alone if reflux control is incomplete. The authors conclude that acid reflux must be controlled to treat BE successfully or the involved segment must be resected. Esophagogastrostomy apparently predisposes to BE.