Psychosocial Adjustment Factors Associated with Child-Parent Violence: The Role of Family Communication and a Transgressive Attitude in Adolescence.

According to official sources, the amounts of children-to-parent violence (CPV) in most advanced countries have been on an increasing trend for more than a decade, which generates great social concern. This phenomenon has also aroused enormous interest among researchers, who have identified risk and protective factors related to adolescent CPV in numerous studies. The aim of the present study was to analyse the relationship between offensive family communication and CPV in adolescence, and the moderating role that two psychosocial adjustment factors may be playing: a positive attitude towards the transgression of rules and psychological distress. A total of 7787 adolescents between 11 and 16 years of age (M = 13.37, SD = 1.34) from secondary schools in the state of Nuevo León (Mexico) participated in the study (51.5% boys, 48.5% girls). Structural equation modelling was performed using structural equation modelling software (EQS). The results showed that offensive family communication has a direct and significant relationship with CPV. It was also observed that there is an indirect relationship between both variables, through the relationships of psychological distress and a positive attitude towards the transgression of rules. The multigroup analysis performed showed gender differences in some of these relationships. Finally, the results and their implications in the field of family intervention are discussed.

Conserved cobalamin acquisition protein 1 is essential for vitamin B12 uptake in both Chlamydomonas and Phaeodactylum.

Microalgae play an essential role in global net primary productivity and global biogeochemical cycling. Despite their phototrophic lifestyle, over half of algal species depend for growth on acquiring an external supply of the corrinoid vitamin B12 (cobalamin), a micronutrient produced only by a subset of prokaryotic organisms. Previous studies have identified protein components involved in vitamin B12 uptake in bacterial species and humans. However, little is known about its uptake in algae. Here, we demonstrate the essential role of a protein, cobalamin acquisition protein 1 (CBA1), in B12 uptake in Phaeodactylum tricornutum using CRISPR-Cas9 to generate targeted knockouts and in Chlamydomonas reinhardtii by insertional mutagenesis. In both cases, CBA1 knockout lines could not take up exogenous vitamin B12. Complementation of the C. reinhardtii mutants with the wild-type CBA1 gene restored B12 uptake, and regulation of CBA1 expression via a riboswitch element enabled control of the phenotype. When visualized by confocal microscopy, a YFP-fusion with C. reinhardtii CBA1 showed association with membranes. Bioinformatics analysis found that CBA1-like sequences are present in all major eukaryotic phyla. In algal taxa, the majority that encoded CBA1 also had genes for B12-dependent enzymes, suggesting CBA1 plays a conserved role. Our results thus provide insight into the molecular basis of algal B12 acquisition, a process that likely underpins many interactions in aquatic microbial communities.

Thiamine metabolism genes in diatoms are not regulated by thiamine despite the presence of predicted riboswitches.

Thiamine pyrophosphate (TPP), an essential co-factor for all species, is biosynthesised through a metabolically expensive pathway regulated by TPP riboswitches in bacteria, fungi, plants and green algae. Diatoms are microalgae responsible for c. 20% of global primary production. They have been predicted to contain TPP aptamers in the 3'UTR of some thiamine metabolism-related genes, but little information is known about their function and regulation. We used bioinformatics, antimetabolite growth assays, RT-qPCR, targeted mutagenesis and reporter constructs to test whether the predicted TPP riboswitches respond to thiamine supplementation in diatoms. Gene editing was used to investigate the functions of the genes with associated TPP riboswitches in Phaeodactylum tricornutum. We found that thiamine-related genes with putative TPP aptamers are not responsive to supplementation with thiamine or its precursor 4-amino-5-hydroxymethyl-2-methylpyrimidine (HMP), and targeted mutation of the TPP aptamer in the THIC gene encoding HMP-P synthase does not deregulate thiamine biosynthesis in P. tricornutum. Through genome editing we established that PtTHIC is essential for thiamine biosynthesis and another gene, PtSSSP, is necessary for thiamine uptake. Our results highlight the importance of experimentally testing bioinformatic aptamer predictions and provide new insights into the thiamine metabolism shaping the structure of marine microbial communities with global biogeochemical importance.

Impact of Bullying-Victimization and Gender over Psychological Distress, Suicidal Ideation, and Family Functioning of Mexican Adolescents.

Bullying victimization is strongly associated with increased psychological distress and suicide in adolescents and poor family functioning. Knowledge of gender differences influencing these factors will improve the prevention of mental problems and suicide in victimized adolescents. A total of 1685 Mexican secondary students, 12-17 years old (m = 13.65), of whom 54% were girls, responded to a standardized scale questionnaire to analyze such differences. Based on the statistical analysis, girls reported significantly lower family functioning and higher psychological distress and suicidal ideation than boys. The cluster analysis classified adolescents into high (5.78%), moderate (24.07%), and no-victimization (69.76%) groups. Boys predominated in the high (3.1%) and moderate-victimization (12.4%) clusters, and girls in the no-victimization group (39.51%). Multivariate statistical analyses found significant differences between the three groups, with the highest means of psychological distress and suicidal ideation and lowest family functioning in the high-victimization group. Only for suicidal ideation, there was an interaction between gender and the degree of victimization, with girls showing a higher increase of suicidal ideation than boys in the same cluster. Conclusions: Early detection and intervention in bullying-victimized adolescents, aiming to decrease psychological distress and suicidal ideation and strengthen family functioning, should consider contextual gender differences for effective prevention of mental health problems and suicide in adolescents.

Exploring the Impact of Terminators on Transgene Expression in Chlamydomonas reinhardtii with a Synthetic Biology Approach.

Chlamydomonas reinhardtii has many attractive features for use as a model organism for both fundamental studies and as a biotechnological platform. Nonetheless, despite the many molecular tools and resources that have been developed, there are challenges for its successful engineering, in particular to obtain reproducible and high levels of transgene expression. Here we describe a synthetic biology approach to screen several hundred independent transformants using standardised parts to explore different parameters that might affect transgene expression. We focused on terminators and, using a standardised workflow and quantitative outputs, tested 9 different elements representing three different size classes of native terminators to determine their ability to support high level expression of a GFP reporter gene. We found that the optimal size reflected the median size of element found in the C. reinhardtii genome. The behaviour of the terminator parts was similar with different promoters, in different host strains and with different transgenes. This approach is applicable to the systematic testing of other genetic elements, facilitating comparison to determine optimal transgene design.

Cybervictimization, Offline Victimization, and Cyberbullying: The Mediating Role of the Problematic Use of Social Networking Sites in Boys and Girls / Cibervictimización, victimización offline y ciberbullying: el rol mediador del uso problemático de las redes sociales virtuales en chicos y chicas

The aim of the present study was to examine the mediating role of the problematic social networking sites use (PSNSU) in the relationship between both cybervictimization and offline victimization and cyberbullying in boys and girls. The sample consisted of 2,011 adolescents (50.67% boys and 49.32% girls), aged between 12 and 18 years old (M = 14.17, SD = 1.47), enrolled in schools in Andalusia, Spain. To examine this objective, moderated mediation model of the PROCESS macro was used. The results showed that both cybervictimization and offline victimization are positively related to cyberbullying directly and indirectly through PSNSU. Moreover, it was observed that boys victimized both online and offline demonstrated a higher involvement in cyberbullying, whereas girls reported a higher PSNSU. However, the PSNSU mediating effect was not moderated by gender. Finally, the results and their practical implications are discussed. (AU)

El objetivo del presente estudio fue examinar el papel mediador del uso problemático de las redes sociales virtuales (UPRSV) en la relación entre la cibervictimización, la victimización offline y el ciberbullying en chicos y chicas. Los participantes fueron 2,011 adolescentes (50.67% chicos y 49.32% chicas), con edades comprendidas entre los 12 y los 18 años (M = 14.17, DT = 1.47), escolarizados en centros educativos de Andalucía (España). Para examinar este objetivo se utilizó el modelo de mediación moderada de la macro de PROCESS. Los resultados obtenidos mostraron que la cibervictimización y la victimización offline se relacionan positivamente con el ciberbullying de manera directa e indirecta, a través del UPRSV. Además, se observó que los chicos victimizados tanto online como offline mostraron una mayor implicación en el ciberbullying mientras que las chicas mostraron un mayor UPRSV. Sin embargo, el efecto mediador del UPRSV no era moderado por el género. Finalmente se discuten los resultados y sus implicaciones prácticas. (AU)

Relationship between School Integration, Psychosocial Adjustment and Cyber-Aggression among Adolescents.

The aim of this study was to analyze the relationships between sociometric types in the classroom-rejected, preferred, neglected, controversial and average-and psychological discomfort, life satisfaction and cyber-aggression, based on the adolescent's gender. 2398 adolescents of both sexes participated in the study (49.8% girls), aged between 12 and 18 years (M = 16.03, SD = 1.91). Multivariate analyses of variance were performed. The results showed significant relationships between sociometric types, life satisfaction and cyber-aggression. Rejected adolescents also showed less satisfaction with life and greater cyber-aggression. Furthermore, the boys, regardless of their sociometric type in the classroom, displayed less psychological distress and less involvement in cyber-aggression. Controversial adolescents also showed greater involvement in cyber-aggression. Finally, programs should be promoted for the prevention of social difficulties in the school, based on the promotion of social integration, not only in the classroom, but also on the Internet.

Bullying Victimization among Mexican Adolescents: Psychosocial Differences from an Ecological Approach.

This transversal study over a random representative sample of 1687 Mexican students attending public and private secondary schools (54% girls, 12-17 years old, M = 13.65. DT = 1.14) aimed to analyze psychosocial differences between victims and non-victims of bullying from the bioecological model. It included individual variables (ontosystem), familiar, community, and scholar factors (microsystem), and gender (macrosystem) to perform a multivariate discriminant analysis and a logistic regression analysis. The discriminant analysis found that psychological distress, offensive communication with mother and father, and a positive attitude toward social norms transgression characterized the high victimization cluster. For the non-victims, the discriminant variables were community implication, positive attitude toward institutional authority, and open communication with the mother. These variables allowed for correctly predicting membership in 76% of the cases. Logistic regression analysis found that psychological distress, offensive communication with the father, and being a boy increased the probability of high victimization, while a positive attitude toward authority, open communication with the mother, and being a girl decrease this probability. These results highlight the importance of open and offensive communication between adolescents and their parents on psychological distress, attitude toward authority, community implication, and bullying victimization.

Development of Novel Riboswitches for Synthetic Biology in the Green Alga Chlamydomonas.

Riboswitches are RNA regulatory elements that bind specific ligands to control gene expression. Because of their modular composition, where a ligand-sensing aptamer domain is combined with an expression platform, riboswitches offer unique tools for synthetic biology applications. Here we took a mutational approach to determine functionally important nucleotide residues in the thiamine pyrophosphate (TPP) riboswitch in the THI4 gene of the model alga Chlamydomonas reinhardtii, allowing us to carry out aptamer swap using THIC aptamers from Chlamydomonas and Arabidopsis thaliana. These chimeric riboswitches displayed a distinct specificity and dynamic range of responses to different ligands. Our studies demonstrate ease of assembly as 5'UTR DNA parts, predictability of output, and utility for controlled production of a high-value compound in Chlamydomonas. The simplicity of riboswitch incorporation in current design platforms will facilitate the generation of genetic circuits to advance synthetic biology and metabolic engineering of microalgae.

Blocking late stages of splicing quickly limits pre-spliceosome assembly in vivo.

Pre-messenger RNA splicing involves multi-step assembly of the large spliceosome complexes that catalyse the two consecutive trans-esterification reactions, resulting in intron removal. There is evidence that proof-reading mechanisms monitor the fidelity of this complex process. Transcripts that fail these fidelity tests are thought to be directed to degradation pathways, permitting the splicing factors to be recycled. While studying the roles of splicing factors in vivo, in budding yeast, we performed targeted depletion of individual proteins, and analysed the effect on co-transcriptional spliceosome assembly and splicing efficiency. Unexpectedly, depleting factors such as Prp16 or Prp22, that are known to function at the second catalytic step or later in the splicing pathway, resulted in a defect in the first step of splicing, and accumulation of arrested spliceosomes. Through a kinetic analysis of newly synthesized RNA, we observed that a second step splicing defect (the primary defect) was rapidly followed by the first step of splicing defect. Our results show that knocking down a splicing factor can quickly lead to a recycling defect with splicing factors sequestered in stalled complexes, thereby limiting new rounds of splicing. We demonstrate that this 'feed-back' effect can be minimized by depleting the target protein more gradually or only partially, allowing a better separation between primary and secondary effects. Our findings indicate that splicing surveillance mechanisms may not always cope with spliceosome assembly defects, and suggest that work involving knock-down of splicing factors or components of other large complexes should be carefully monitored to avoid potentially misleading conclusions.

Parental Socialization, School Adjustment and Cyber-Aggression among Adolescents.

The objective of the present study is to analyse the relationships between parental socialization styles-indulgent, authoritarian, authoritative and negligent, school adjustment (social integration, academic competence and family involvement) and cyber-aggression (direct and indirect) in adolescents. Participating in this study were 1304 Spanish students of both sexes (53.1% girls), aged between 12 and 18 years (M = 13.87, SD = 1.33). Multivariate analyses of variance were performed. The results showed significant relationships between parental socialization styles, school adjustment and cyber-aggression. It was observed that adolescents from indulgent and authoritative families showed greater academic competence and greater family involvement. Additionally, the children from authoritarian families displayed greater involvement in direct and indirect cyber-aggression behaviours. The results obtained and their implications are discussed in the final section.

Relationships between Parental Socialization Styles, Empathy and Connectedness with Nature: Their Implications in Environmentalism.

Parents exert a strong influence on several adjustment outcomes. However, little is known about their influence on adolescents' connectedness with the environment. This study examined the relationships between parenting styles, empathy and connectedness with the environment. The two-dimensional socialization model was used with four resulting styles: Indulgent, authoritative, neglectful and authoritarian. The sample comprised 797 adolescents (52.7% girls) from six public secondary schools who were aged between 12 and 16 years (M = 13.94, SD = 1.28). The results showed significant relationships between parental socialization styles, empathy and connectedness with nature. It was also observed that adolescents from indulgent and authoritative families showed higher levels of empathy and connectedness with the environment than adolescents raised by authoritarian and neglectful parents, with males from such families consistently presenting the lowest levels of empathy and connectedness, which was not the case among women. Additionally, women, regardless of the parental style in which they had been educated, showed greater cognitive and emotional empathy with the natural environment, while adolescents raised in indulgent and authoritative families displayed higher levels of empathy and connectedness than those with authoritarian and neglectful parents. These results suggest that indulgent and authoritative styles are stronger enablers of empathy and connectedness with nature.

Tuning Degradation to Achieve Specific and Efficient Protein Depletion.

The plant auxin binding receptor, TIR1, recognizes proteins containing a specific auxin-inducible degron (AID) motif in the presence of auxin, targeting them for degradation. This system is exploited in many non-plant eukaryotes, such that a target protein, tagged with the AID motif, is degraded upon auxin addition. The level of TIR1 expression is critical; excessive expression leads to degradation of the AID-tagged protein even in the absence of auxin, whereas low expression leads to slow depletion. A ß-estradiol-inducible AID system was created, with expression of TIR1 under the control of a ß-estradiol inducible promoter. The level of TIR1 is tunable by changing the time of incubation with ß-estradiol before auxin addition. This protocol describes how to rapidly deplete a target protein using the AID system. The appropriate ß-estradiol incubation time depends on the abundance of the target protein. Therefore, efficient depletion depends on optimal timing that also minimizes auxin-independent depletion.

A fast and tuneable auxin-inducible degron for depletion of target proteins in budding yeast.

The auxin-inducible degron (AID) is a useful technique to rapidly deplete proteins of interest in nonplant eukaryotes. Depletion is achieved by addition of the plant hormone auxin to the cell culture, which allows the auxin-binding receptor, TIR1, to target the AID-tagged protein for degradation by the proteasome. Fast depletion of the target protein requires good expression of TIR1 protein, but as we show here, high levels of TIR1 may cause uncontrolled depletion of the target protein in the absence of auxin. To enable conditional expression of TIR1 to a high level when required, we regulated the expression of TIR1 using the ß-estradiol expression system. This is a fast-acting gene induction system that does not cause secondary effects on yeast cell metabolism. We demonstrate that combining the AID and ß-estradiol systems results in a tightly controlled and fast auxin-induced depletion of nuclear target proteins. Moreover, we show that depletion rate can be tuned by modulating the duration of ß-estradiol preincubation. We conclude that TIR1 protein is a rate-limiting factor for target protein depletion in yeast, and we provide new tools that allow tightly controlled, tuneable, and efficient depletion of essential proteins whereas minimising secondary effects.

Percepción de inseguridad, victimización y restricciones en la vida cotidiana en función del ciclo vital, en Morelos, México / Perception of insecurity, victimization and restrictions in daily life according to the life cycle, in Morelos, Mexico / Percepção da insegurança, vitimização e as limitações na vida diária baseada no ciclo vital, em Morelos, México

Resumen Los estudios sobre percepción de inseguridad, victimización y restricciones en la vida cotidiana en países con altos índices de criminalidad son escasos. Objetivo: examinar la percepción de inseguridad, victimización y variaciones de las rutinas en función de la edad. Método: se ha realizado una adaptación de la Encuesta Nacional sobre Victimización y Percepción de Inseguridad (ENVIPE). Participaron 8.170 sujetos de ambos sexos (49,9 % mujeres y 50,1 % hombres), de entre 12 y 75 años, residentes en el Estado de Morelos, seleccionados a partir de un muestreo probabilístico estratificado y proporcional. Respecto a la edad, se establecieron los siguientes intervalos en función de las distintas etapas del ciclo vital: [12-17 años] 24 %, [18-20 años] 8 %, [21-30 años] 14 %, [31 y 40 años] 14 %, [41 y 60 años] 20 % y [61 o más años] 20 %. Resultados: indicaron diferencias significativas en la percepción de inseguridad, victimización y restricciones en las actividades cotidianas en función de la edad. Los adolescentes informaron de mayor percepción de inseguridad y de menos restricciones en su vida cotidiana. También, los adolescentes y los mayores de 61 años presentaron una menor victimización. Conclusión: los adolescentes constituyen el grupo de mayor vulnerabilidad para la victimización, perciben mayor inseguridad y realizan menos cambios en sus rutinas para protegerse de la delincuencia. Finalmente, se discuten los resultados.

Abstract Studies on the perception of insecurity, victimization and restrictions in daily life in countries with high crime rates are scarce. Objective: examining the awareness of insecurity and victimization, and the routine variations taking place according to age. Method: an adaptation has been made of the National Survey on Victimization and Perception of Insecurity (ENVIPE). A total of 8,170 subjects of both sexes (49.9% women and 50.1% men), between 12 and 75 years old, residents in the State of Morelos, selected from a stratified and proportional probabilistic sampling. Regarding age, the following intervals were established depending on the different stages of the life cycle: [12-17 years] 24%, [18-20 years] 8%, [21-30 years] 14%, [31 and 40 years] 14%, [41 and 60 years] 20% and [61 or above] 20%. Results: Significant differences in the perception of insecurity, victimization and restrictions in daily activities based on age were shown. Adolescents reported greater insight with respect to insecurity and fewer restrictions in their daily lives. Also, adolescents and those over 61 years of age presented lower victimization. Conclusion: adolescents are the most vulnerable group for victimization; they perceive higher uncertainty and generally introduce fewer changes in their routines to protect themselves from crime. Finally, the results are discussed.

Resumo Os estudos sobre a percepção da insegurança, vitimização e as limitações na vida diária nos países com índices elevados de criminalidade são escassos. Objetivo: examinar a percepção da insegurança, vitimização e as variações das rotinas baseadas na idade. Método: uma adaptação da Escola Nacional sobre Vitimização e Percepção de Insegurança (ENVIPE) foi realizada. 8.170 sujeitos de ambos os sexos participaram (49.9% mulheres e 50.1% homens), entre e 75 anos, residentes no estado de Morelos, selecionados de uma amostra probabilística estratificada e proporcional. Com respeito à idade, os seguintes intervalos baseados nos diferentes estágios do ciclo vital forma estabelecidos: [12-17 anos] 24%, [18-20 anos] 8%, [21-30 anos] 14%, [31 e 40 anos] 14%, [41 e 60 anos] 20% e [61 ou mais anos] 20%. Resultados: indicaram diferenças significativas na percepção de insegurança, vitimização e as limitações nas atividades diárias baseadas na idade. Os adolescentes informaram uma maior percepção de insegurança e de menos limitações em sua vida diária. Também, os adolescentes e maiores de 61 anos apresentaram uma vitimização menor. Conclusão: os adolescentes constituem o grupo de maior vulnerabilidade para a vitimização, percebem maior insegurança e fazem menos mudanças em suas rotinas para proteger-se da delinquência. Finalmente, os resultados são discutidos.

[Spanish adolescents' low perception of risk in alcohol consumption]. / Baja percepción de riesgo en el consumo de alcohol en adolescentes españoles.

According to recent studies, Spanish adolescents show low perception of risk in alcohol consumption. The current study aims to analyze the factors that favor this low perception based on the opinion of a group of 32 professional experts on adolescence, family, school, mass media, and local policies. A qualitative methodology was used, based on Grounded Theory, using information from 5 focus groups guided by semi-structured interviews. Twelve factors or subcategories were identified, grouped in 4 general categories: short-term risk, immediacy, and perception of invulnerability ("adolescent thinking" category); benevolent view of alcohol, normalization of consumption, and alcohol-entertainment binomial ("social norms" category); parents' habitual consumption, verbal/non-verbal inconsistency in parental model, risk-free consumption depicted in the mass media, consumption with positive results in the media ("social models" category); and excessive health content, long-term risk ("preventive discourse" category). After discussing the results in the context of the current scientific literature, the article offers various proposals for increasing risk perception in adolescents: stronger impact of contents on short-term risks of alcohol; educational strategies targeted to adolescents to include agents of socialization, especially parents; and policies centered on the substance and reduction of supply.

Understanding Rejection between First-and-Second-Grade Elementary Students through Reasons Expressed by Rejecters.

Objective: The aim of this research was to obtain the views of young children regarding their reasons for rejecting a peer. Method: To achieve this goal, we conducted a qualitative study in the context of theory building research using an analysis methodology based on Grounded Theory. The collected information was extracted through semi-structured individual interviews from a sample of 853 children aged 6 from 13 urban public schools in Spain. Results: The children provided 3,009 rejection nominations and 2,934 reasons for disliking the rejected peers. Seven reason categories emerged from the analysis. Four categories refer to behaviors of the rejected children that have a cost for individual peers or peer group such as: direct aggression, disturbance of wellbeing, problematic social and school behaviors and dominance behaviors. A further two categories refer to the identities arising from the preferences and choices of rejected and rejecter children and their peers: personal identity expressed through preferences and disliking, and social identity expressed through outgroup prejudices. The "no-behavior or no-choice" reasons were covered by one category, unfamiliarity. In addition, three context categories were found indicating the participants (interpersonal-group), the impact (low-high), and the subjectivity (subjective-objective) of the reason. Conclusion: This study provides researchers and practitioners with a comprehensive taxonomy of reasons for rejection that contributes to enrich the theoretical knowledge and improve interventions for preventing and reducing peer rejection.

Baja percepción de riesgo en el consumo de alcohol en adolescentes españoles / Spanish adolescents' low perception of risk in alcohol consumption / Baixa percepção de risco entre adolescentes espanhóis em relação ao consumo de álcool

Según los estudios recientes, los adolescentes españoles muestran una baja percepción del riesgo asociado al consumo de alcohol. El objetivo de la presente investigación es analizar los factores que favorecen esta baja percepción a partir de la opinión de un grupo de 32 profesionales/expertos en adolescencia, familia, escuela, medios de comunicación y políticas locales. Se utilizó una metodología cualitativa, fundamentada en Grounded Theory, a partir de la información obtenida mediante 5 grupos de discusión guiados por entrevistas semiestructuradas. Se identificaron 12 factores o subcategorías agrupados en 4 categorías generales: riesgo a corto plazo, inmediatez y percepción de invulnerabilidad (categoría "pensamiento adolescente"); concepción benévola del alcohol, normalización del consumo y binomio alcohol-diversión (categoría "normas sociales"); consumo habitual en los padres, inconsistencia verbal-no verbal en el modelado parental, consumo sin riesgo en los medios, consumo con resultados positivos en los medios (categoría "modelos sociales"); excesivo contenido sanitario, riesgo a largo plazo (categoría "discurso preventivo"). Tras discutir los resultados en el contexto de la literatura científica actual, se realizan varias propuestas con el objetivo de aumentar la percepción del riesgo en los adolescentes: incidir con mayor fuerza en contenidos sobre los riesgos a corto plazo del alcohol; orientar las estrategias educativas dirigidas al adolescente, también hacia los agentes de socialización, especialmente los padres; incidir en políticas centradas en la sustancia y en reducir la oferta.

According to recent studies, Spanish adolescents show low perception of risk in alcohol consumption. The current study aims to analyze the factors that favor this low perception based on the opinion of a group of 32 professional experts on adolescence, family, school, mass media, and local policies. A qualitative methodology was used, based on Grounded Theory, using information from 5 focus groups guided by semi-structured interviews. Twelve factors or subcategories were identified, grouped in 4 general categories: short-term risk, immediacy, and perception of invulnerability ("adolescent thinking" category); benevolent view of alcohol, normalization of consumption, and alcohol-entertainment binomial ("social norms" category); parents' habitual consumption, verbal/non-verbal inconsistency in parental model, risk-free consumption depicted in the mass media, consumption with positive results in the media ("social models" category); and excessive health content, long-term risk ("preventive discourse" category). After discussing the results in the context of the current scientific literature, the article offers various proposals for increasing risk perception in adolescents: stronger impact of contents on short-term risks of alcohol; educational strategies targeted to adolescents to include agents of socialization, especially parents; and policies centered on the substance and reduction of supply.

De acordo com estudos recentes, os adolescentes espanhóis mostram baixa percepção de risco em relação ao consumo de álcool. O estudo tem como objetivo analisar os fatores associados com a baixa percepção de risco, com base nas opiniões de um grupo de 32 especialistas em adolescência, família, escola, mídia e políticas locais. Foi utilizada uma metodologia qualitativa, baseada na Teoria Fundamentada, usando informações de cinco grupos focais orientados por entrevistas semi-estruturadas. Foram identificados 12 fatores ou subcategorias, agrupados em quatro categorias gerais: risco no curto prazo, imediatismo e percepção de invulnerabilidade (categoria de "pensamento adolescente"); visão benevolente em relação ao álcool, normalização do consumo e binômio álcool-entretenimento (categoria de "normas sociais"); consumo habitual pelos pais, inconsistência na comunicação verbal versus não verbal no modelo representado pelos pais, consumo isento de risco retratado pela mídia, consumo com resultados positivos na mídia (categoria de "modelos sociais") e excesso de conteúdo relacionado à saúde, risco no longo prazo (categoria de "discurso preventivo"). Depois de discutir os resultados no contexto da literatura científica atual, o artigo oferece várias propostas para aumentar a percepção de risco entre os adolescentes: impacto mais forte de conteúdos sobre os riscos do álcool no curto prazo; estratégias educativas orientadas aos adolescentes para incluir os agentes da socialização, especialmente os pais, além de políticas centradas na substância e na redução da oferta.

[Satisfaction with life, victimization, and perception of insecurity in Morelos]. / Satisfacción con la vida, victimización y percepción de inseguridad en Morelos, México.

OBJECTIVE: To examines the influence of victimization, perceived insecurity and restrictions on daily routines in life satisfaction. MATERIALS AND METHODS: Participants were 7535 (50.2% men) aged between 12 and 60, selected from a proportional stratified sampling. MANOVA and polytomous logistic regression model were calculated. RESULTS: We found significant differences in victimization, perceived insecurity and restrictions on daily routines in relation with life satisfaction levels. Also, physical protective measures, control of personal information, perception of insecurity in public areas and restrictions on daily routines were related to lower levels of satisfaction with life. CONCLUSIONS: Lowest levels of satisfaction with life were associated with victimization, perception of insecurity in public areas, and restrictions on daily routines.

Satisfacción con la vida, victimización y percepción de inseguridad en Morelos, México / Satisfaction with life, victimization, and perception of insecurity in Morelos

Objetivo. Examinar la influencia que ejercen la victimización, la percepción de inseguridad y los cambios en las rutinas en la satisfacción con la vida. Material y métodos. Participaron 7 535 sujetos (50.2% hombres) de entre 12 y 60 años, seleccionados a partir de un muestreo estratificado proporcional. Se calculó un análisis multivariado de la varianza (Manova) y un análisis de regresión logística politómica. Resultados. Se observaron diferencias significativas en victimización, percepción de inseguridad y restricciones en actividades cotidianas en función del grado de satisfacción con la vida. Además, las medidas de protección, la percepción de inseguridad y las restricciones en actividades cotidianas se relacionaron con la satisfacción con la vida. Conclusiones. Un bajo nivel de satisfacción con la vida se asoció con haber sido víctima, con la percepción de inseguridad en espacios públicos y con la adopción de medidas de protección física y control de la información.

Objective. To examines the influence of victimization, perceived insecurity and restrictions on daily routines in life satisfaction. Materials and methods. Participants were 7535 (50.2% men) aged between 12 and 60, selected from a proportional stratified sampling. MANOVA and polytomous logistic regression model were calculated. Results. We found significant differences in victimization, perceived insecurity and restrictions on daily routines in relation with life satisfaction levels. Also, physical protective measures, control of personal information, perception of insecurity in public areas and restrictions on daily routines were related to lower levels of satisfaction with life. Conclusions. Lowest levels of satisfaction with life were associated with victimization, perception of insecurity in public areas, and restrictions on daily routines.