RESUMO
A method was elaborated to construct combined artificial immunogens mimicking virus particles. The gist was exposing protein antigenic determinants of one virus on the particle surface and delivering plasmids with genes for antigenic proteins of another virus to specialized immune cells. Such immunogens were constructed and shown to induce biosynthesis of specific antibodies against HIV-1 and the tick-borne encephalitis virus. The level and duration of the humoral and cell responses were assayed.
Assuntos
Desenho de Fármacos , Vacinas Sintéticas/genética , Vacinas Sintéticas/imunologia , Vacinas contra a AIDS/genética , Vacinas contra a AIDS/imunologia , Animais , Anticorpos/imunologia , Anticorpos/metabolismo , Formação de Anticorpos , Vírus da Encefalite Transmitidos por Carrapatos/imunologia , Epitopos/genética , Proteína do Núcleo p24 do HIV/genética , Proteína do Núcleo p24 do HIV/imunologia , HIV-1/imunologia , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Eletrônica , Plasmídeos/genética , Vacinas Sintéticas/farmacologia , Proteínas Virais/química , Proteínas Virais/genética , Proteínas Virais/imunologiaAssuntos
Genes Sintéticos , Modelos Imunológicos , Proteínas Recombinantes , Vacinas Combinadas/biossíntese , Vacinas Combinadas/imunologia , Vacinas Sintéticas/biossíntese , Vacinas Sintéticas/imunologia , Animais , Anticorpos/sangue , Anticorpos/imunologia , Vírus da Encefalite Transmitidos por Carrapatos/imunologia , Escherichia coli , Técnicas Imunoenzimáticas , Camundongos , Camundongos Endogâmicos BALB C , Proteínas/genética , Proteínas/imunologia , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/imunologia , Células Th1/imunologia , Células Th2/imunologia , VacinaçãoRESUMO
A method for designing molecular constructions of combined artificial immunogens mimicking viral particles is proposed. Using this method, it is possible to expose antigenic determinants of any viral protein on the surface of such particles and to deliver plasmids containing genes encoding the synthesis of protein antigens to target cells. This approach was used to create the constructions of combined artificial immunogens inducing the production of specific antibodies to HIV-1 and to evaluate the extent and duration of B-cellular immune response depending on the way of antigen exposure to immunocompetent cells.
