Knowledge about relationship of diets with non-communicable disease and prospects for amelioration by consuming mushrooms in South-East Nigeria.

Background: Knowledge plays a key role in shaping people's attitudes and behaviors to adopt healthy lifestyles, which is important in any program of NCD prevention. There is no data about how much the general public in South-East Nigeria (SE) knows about how their diets may dispose or help them prevent NCDs. Aim: This study aims to assess knowledge available on the relationship between NCDs and diets/foods including mushrooms among the general public of SE Nigeria. Methods: Data was collected using the survey questionnaire and interview method in the five SE States: Abia, Anambra, Ebonyi, Enugu, and Imo States. Simple descriptive statistics were used to analyze data. Results: A total of 846 responses were received from 1000 questionnaires. Proportion of respondents who knew (p^yes) about a relationship between diets and NCDs was 51.5%. p^yes was significantly higher among males than females and increased with age and level of education of respondents. It also varied among the States of SE Nigeria. Knowledge scores based on a scale of 0-5 revealed that respondents knew more about foods, which can reduce (2.3 ± 0.102) e.g., fruits/vegetables, than can increase (1.9 ± 0.096) e.g., sugary/starchy foods, the risk of NCDs. Varying proportions of respondents in all States of the SE knew that mushroom consumption can ameliorate NCDs. Conclusion: This study reveals that half of population of SE Nigeria knows about the relationship between diets and NCDs. A significant proportion also knows that mushroom consumption can ameliorate NCDs suggesting prospects of its utilization in preventing NCDs.

Draft Genome Sequence of Citrobacter cronae Awk (Sequence Type 880), Associated with Edible Snails Commercially Available in Awka, Nigeria.

We describe the draft genome sequence and annotation of Citrobacter cronae strain Awk (sequence type 880), recovered from fresh edible snails (Achatina achatina) commercially available in Awka Metropolis, Nigeria. The genome contains 4,629 protein-coding genes, 107 RNA-coding genes, and several antimicrobial resistance genes, including blaCMY-98 and qnrB12.

First Report of the Presence of Enterotoxin Gene in Coagulase-negative Staphylococci Recovered from Meat of Snails (Achatina Achatina).

Introduction: It has been suggested that coagulase-negative staphylococci can serve as reservoirs of virulence genes for other bacteria. This study assessed the presence of such genes in selected isolates recovered from meat of the giant African snail (Achatina achatina). Material and Methods: Virulence genes were detected using a polymerase chain reaction targeting specific primers. Two representative isolates were identified using 16S rRNA gene sequencing. Results: The results showed that the staphylococcal enterotoxin A gene (sea) was present in five out of the eight isolates studied. The isolates expressed resistance mainly to three antibiotics: chloramphenicol, norfloxacin and cloxacillin in descending order of incidence. Most importantly, the Staphylococcus sciuri isolate NEDU 181, in addition to being resistant to the three aforementioned antibiotics, also harboured the sea gene. Conclusion: Our findings demonstrate, for the first time, the presence of toxigenic and antibiotic-resistant coagulase-negative Staphylococcus spp. in commercially-available fresh snail meat. With staphylococcal enterotoxin A known to survive cooking temperature, this presents a food safety concern.

Genome Sequence of OXA-726-Encoding Aeromonas dhakensis Igbk (Sequence Type 1171) from an Edible Snail Traded in Nigeria.

Aeromonas dhakensis is the most virulent Aeromonas species pathogenic to both animals and humans. The degree of its risk to health seems masked by misidentifications. We present the genome sequence of A. dhakensis Igbk (sequence type 1171), associated with snails, harboring the OXA-726 gene in the chromosome.

Conversion of cassava wastes for biofertilizer production using phosphate solubilizing fungi.

Two fungi characterized as Aspergillus fumigatus and Aspergillus niger, isolated from decaying cassava peels were used to convert cassava wastes by the semi-solid fermentation technique to phosphate biofertilizer. The isolates solubilized Ca(3)(PO(4))(2), AlPO(4) and FePO(4) in liquid Pikovskaya medium, a process that was accompanied by acid production. Medium for the SSF fermentation was composed of 1% raw cassava starch and 3% poultry droppings as nutrients and 96% ground (0.5-1.5mm) dried cassava peels as carrier material. During the 14days fermentation, both test organisms increased in biomass in this medium as indicated by increases in phosphatase activity and drop in pH. Ground cassava peels satisfied many properties required of carrier material particularly in respect of the organisms under study. Biofertilizer produced using A. niger significantly (p<.05) improved the growth of pigeon pea [Cajanus cajan (L.) Millsp.] in pot experiments but product made with A. fumigatus did not.

Improvement of organoleptic quality of retted cassava products by alkali pretreatment of roots and addition of sodium nitrate during retting.

Alkali pretreatment of cassava roots before retting and addition of sodium nitrate during retting were used to manipulate the metabolism of microorganisms involved in cassava (Manihot esculenta Crantz) retting, as a method for removing the characteristic offensive odour of retted cassava products. Odour was assessed by organoleptic methods. The characteristics of fermentation of cassava by the traditional method (control) were as follows; aerobic mesophilic count (APC) on nutrient agar (NA) at 30 degrees C/48 h, attained a maximum of 2.3 x 10(7)/ml retting juice while counts on de Man Rogosa and Sharpe agar (MRS) at 30 degrees C/48 h were 1.6 x 10(8)/ml. Maximum titrable acidity was 0.062% lactic acid by weight of retting juice. Cassava was retted in 3 days and the product exhibited characteristic offensive odour. Addition of NaNO3 into retting water effectively removed odour at a concentration of 0.3 g/l. Maximum APC on NA/30 degrees C/48 h was 6.8 x 10(6)/ml. Counts on MRS/30 degrees C/48 h exceeded 2.4 x 10(9)/ml. Retting was complete in 3 days with a final titrable acidity of 0.068% of retting juice. Removal of odour likely resulted from selection of homo-fermentative lactic acid bacteria, thus producing mostly odourless lactic acid. Alkali pretreatment of roots before retting was efficacious in removing odour at a concentration of 10 g/l for 30 min. This fermentation was characterized by APC on NA/30 degrees C/48 h of 5.4 x 10(6)/ml; MRS/30 degrees C/48 h reached a maximum of only 10 x 10(4)/ml and correspondingly low titrable acidity of 0.003%. Low counts of lactic acid bacteria correlate well with the absence of odour in this sample. Both treatments did not adversely affect the detoxification process, yielding "foo-foo" with HCN levels lower than 10 mg/kg. Residual nitrates and nitrites of 30 mg/kg in the sodium nitrate-treated sample were also within the safe limits of 156 mg/kg allowed in many countries. Organoleptically improved samples were acceptable to the public.