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1.
Proteomics ; 14(12): 1543-6, 2014 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-24723484

RESUMO

Yellow lupin (Lupinus luteus L.) is a legume crop containing a large amount of protein in its seeds. In this study, we constructed a seed-protein catalog to provide a foundation for further study of the seeds. A total of 736 proteins were identified in 341 2DE spots by nano-LC-MS/MS. Eight storage proteins were found as multiple spots in the 2DE gels. The 736 proteins correspond to 152 unique proteins as shown by UniRef50 clustering. Sixty-seven of the 152 proteins were associated with KEGG-defined pathways. Of the remaining proteins, 57 were classified according to a GO term. The functions of the remaining 28 proteins have yet to be determined. This is the first yellow lupin seed-protein catalog, and it contains considerably more data than previously reported for white lupin (L. albus L.).


Assuntos
Lupinus/metabolismo , Proteínas de Plantas/metabolismo , Proteoma/metabolismo , Proteômica/métodos , Sementes/metabolismo , Cromatografia Líquida , Eletroforese em Gel Bidimensional , Proteínas de Plantas/genética , Sementes/química , Espectrometria de Massas em Tandem
2.
PLoS One ; 8(11): e80369, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24278278

RESUMO

The need of quality protein in the aquaculture sector has forced the incorporation of alternative plant proteins into feeding diets. However, most plant proteins show lower digestibility levels than fish meal proteins, especially in carnivorous fishes. Manipulation of protein content by plant breeding can improve the digestibility rate of plant proteins in fish, but the identification of low digestibility proteins is essential. A reduction of low digestibility proteins will not only increase feed efficiency, but also reduce water pollution. Little is known about specific digestible protein profiles and/or molecular identification of more bioavailable plant proteins in fish diets. In this study, we identified low digestibility L. luteus seed proteins using Atlantic salmon (Salmo salar) crude digestive enzymes in an in vitro assay. Low digestibility proteins were identified by comparing SDS-PAGE banding profiles of digested and non-digested lupin seed proteins. Gel image analysis detected a major 12 kDa protein band in both lupin meal and protein isolate digested products. The 12 kDa was confirmed by 2D-PAGE gels and the extracted protein was analyzed with an ion trap mass spectrometer in tandem mass mode. The MS/MS data showed that the 12 kDa low digestibility protein was a large chain δconglutin, a common seed storage protein of yellow lupin. Comparison of the protein band profiles between lupin meal and protein isolates showed that the isolatation process did not affect the low digestibility of the 12 kDa protein.


Assuntos
Ração Animal , Lupinus/embriologia , Salmão , Sementes , Espectrometria de Massas em Tandem/métodos , Sequência de Aminoácidos , Animais , Eletroforese em Gel Bidimensional , Lupinus/química , Dados de Sequência Molecular , Proteínas de Plantas/química
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