Anti-atherogenic and cardio-protective properties of sweet melon (Cucumis melo. L. Inodorus) seed extract on high fat diet induced obesity in male wistar rats.

BACKGROUND: Cucumis melon is a medicinal plant with multiple pharmacological properties such as anti-inflammatory, antioxidant, and diuretic effects. An increasing body of scientific evidence established the anti-diabetic/anti-obesity effects of Cucumis melo in humans, mice, and hamster models. However, there are no tangible reports on its ability to prevent cardiovascular complications following diet-induced obesity. The anti-atherogenic and cardioprotective effects of the Methanolic extract of Cucumis melo. L. Inodorus seeds on a high-fat diet (HFD)-induced obese rats was assessed in this study.  METHODS: Forty male Wistar rats were randomly divided into five groups, (n = 8/group); i.e., Normal (N), HFD, HFD + 50 mg/kg b.w. of MCMs (Methanolic extract of Cucumis melon seeds), HFD + 100 mg/Kg b.w. of MCMs and HFD + 200 mg/kg b.w. of MCMs. The experimental animals were anaesthetized and sacrificed after 10 weeks, and blood samples and heart tissue were collected for further analysis. Using the Graph Pad Prism version 5.0, the results expressed as Mean ± SD was tested using the one-way ANOVA to show intergroup differences, followed by Bonferonni 's post hoc test. The level of significance was determined at P ≤ 0.05. RESULTS: MCMs significantly (P < 0.05) reduced body weight, adiposity index, total fat mass, low-density lipoprotein cholesterol (LDL-c), and total cholesterol (TC) compared with the HFD obese groups MCMs caused a significant reduction in the body weight, total fat mass, adiposity index, low-density lipoprotein cholesterol (LDL-c), and total cholesterol (TC) when compared to the animals in HFD obese groups. Also, the Atherogenic index of plasma (AIP), Castelli index and, malondialdehyde (MDA) significantly (P < 0.05) decreased in MCMs treated groups compared to the HFD obese group. The catalase, protein, and HDL levels were significantly increased in MCMs treated groups compared to HFD-obese animals. Expression of nitric oxide in the form of nitrite in the heart tissue significantly increased in the MCMs treated compared to the HFD-obese rats, with the majority of the positive results recorded at 100 mg/Kg b.w. of MCMs. CONCLUSIONS: MCMs have anti-atherogenic and Cardio-protective properties on High Fat Diet-Induced Obesity in Male rats via an antioxidant and nitric oxide-dependent mechanism. Further study is recommended to evaluate the molecular mechanisms to which these anti-atherogenic and cardio-protective actions can be attributed and exploit the GCMS result in the development of drug candidates.

Women in Neurosurgery: Historical Path to Self-Segregation and Proposal for an Integrated Future.

Despite the rising percentage of women accessing the medical profession over the last few decades, surgical specialties are still largely male-dominated; in particular, a remarkable gender disparity is evident in neurosurgery, where only 19% of practitioners are females. Although women may be reluctant to choose a challenging specialty like neurosurgery due to concerns around how to balance family and career, it must be admitted that prejudices against female neurosurgeons have been deeply rooted for long, prompting many to give up and switch track to less demanding subspecialties. Among those who have persisted, many, if not most, have experienced difficulties in career progression and received unequal treatment in comparison with their male counterparts. In 1989, a group of 8 female neurosurgeons founded Women in Neurosurgery (WINS), an organization that aimed to guarantee inclusivity in neurosurgery, encouraging a better and more egalitarian working environment. Thereafter, WINS sessions were regularly promoted at international conferences, offering female neurosurgeons a platform to report issues related to gender discrimination. Over recent years, the mission of WINS sessions in national and international conferences has taken an unexpected deviation; they have progressively become supplementary scientific sessions with only women neurosurgeons as speakers, thus paving the road to a form of self-segregation. This tendency has also resulted in the establishment of sections of only female neurosurgeons within some national societies. Although there remains a faction that fiercely supports the WINS mindset of reserved spaces for women, such segregation is an upsetting prospect for those who believe that science and professionalism have no gender; a growing part of the global neurosurgical community believes that the conception of a "female neurosurgery" and a "male neurosurgery" is misguided and counterproductive and consider the existence of the WINS as anachronistic and no longer necessary.

Pattern of Self-care practices among type 2 diabetes patients in Southwest, Nigeria.

BACKGROUND: Self-care practices in individuals with diabetes are important skills required to effectively prevent, manage, and limit complications associated with diabetes since patients spend considerably less time with health care providers than spend alone to manage their diabetes condition. AIMS: The aim of the study was to assess self-cate practices and their determinants among patients with type 2 diabetes. Hence, this study aimed at assessing self-care practices and their determinants among patients with type 2 diabetes. MATERIALS AND METHODS: This cross-sectional, descriptive, multi-center study was conducted among 348 type 2 diabetes patients selected from six tertiary hospitals in Southwest Nigeria. Descriptive statistical analysis was employed for categorical and continuous variables and multivariable logistic regression assessed association between determinant factors and adherence to self-monitoring of blood glucose (SMBG). RESULTS: Of the study participants, 83.1%, 66.9%, 28.4%, and 27.9% adhered to prescribed medications, physical exercise, had meal plans incorporated into their diabetes management and SMBG, respectively. There was a statistically significant association between male gender, duration of diabetes, and previous episode of hypoglycemia with adherence to SMBG practices while lower educational level and use of insulin were associated with less likelihood of adherence to prescribed medications. CONCLUSION: The degree to which individuals with diabetes adhered to recommended self-care practice components were less than satisfactory especially SMBG, physical activity, and having meal plans.

Antioxidant and the ameliorating effect of Allium cepa (Onion) fortified feed against potassium bromate induced oxidative damage in Wistar rats.

Allium cepa Linn (Onion) Organosulfuric compounds and phytonutrients have medicinal benefits. The study estimated the antioxidant effect of Allium cepa in fortified feed against oxidative damage caused by potassium bromate. Commercial feed was fortified by substituting 10 %, 20 %, and 30 % of rat's daily ration with the respective portion of pulverized Allium cepa. Potassium bromate was administered orally to the rats in all the groups except rats in the normal control. The rats in the test groups were allowed access to the fortified feed ad libitum. The animals were sacrificed; consequently, the serum, liver, and kidney were obtained for biochemical assay and histological assessment. The percentage composition of some amino acids and some proximate were higher in the fortified feed. Furthermore, Malondialdehyde (MDA) level in the liver, kidney, and serum decreased significantly (P ≤ 0.05) in rats fed with fortified feed compared to administered only Potassium bromate. Similarly, the concentration of total protein increased significantly (P ≤ 0.05) in the liver, kidney, and serum of the animals fed with fortified feed. The hematology result was normal in rats fed with fortified feed. The liver and kidney cell architecture was normal in animals fed with fortified feed. Allium cepa may have conferred protection and amelioration to oxidative damage by potassium bromate in rats.

Aqueous extract of Solanum macrocarpon Linn leaves abates hyperglycaemia and expression of glucose transporters gene in alloxan-induced diabetic rats.

PURPOSE: In this study, antihyperglycaemic and level of gene expression of glucose transporters in alloxan-induced diabetic rats administered aqueous extract of S. macrocarpon leaves were assessed. METHOD AND RESULTS: Diabetes was induced by a single intraperitoneal (I.P) injection of freshly prepared alloxan. The animals were divided into six groups, euthanized on the fourteenth day of the experiment and different hyperglycaemic parameters were evaluated. Administration of different doses of the plant extract significantly (p < 0.05) reduced the fasting blood glucose level, glycated haemoglobin, serum lipid profiles, lipid peroxidation, and glucose-6-phosphatase. There was a significant (p < 0.05) increase in liver glycogen content, antioxidant enzyme activities, hexokinase activity, and expression of glucose transporter genes (GLUT-2 and GLUT-4) in diabetic rats administered different doses of S. macrocarpon. CONCLUSION: It can be concluded that the aqueous extract of S. macrocarpon leaves could be helpful in the management of diabetes mellitus and its metabolic complications.

Prevalence and Determinants of Complementary and Alternative Medicine (CAM) Use among Diabetes Patients in Southwestern Nigeria.

INTRODUCTION: The epidemic of diabetes mellitus (DM) in African countries demands a critical assessment and review of patients' management protocols and their self-care habits. The objective of this research was to evaluate the pattern of usage of CAM, its role in the management of diabetes and possible determinants of its use in patients with diabetes. METHODS: Subjects were recruited from six selected hospitals from four of the six States in South western, Nigeria. Participants were consecutively recruited from the diabetes clinics of the selected hospitals. Participants completed a multicomponent questionnaire which comprised three sections. RESULTS: The majority of the subjects were females (57.7%) and of Yoruba extraction (89.7%). Their mean age was 62.58±11.80 years and their mean duration of diabetes was 7.95±6.41 years (range 1-36 years). Two hundred and forty one (62.1%) of the subjects reported the use of at least one type of CAM of which 213(88.4%) indicated using CAM as complementary to regular conventional anti-diabetic medications, while in the remaining 28 (11.6%) CAM users, it served as alternative medicine. The predictors of CAM use included male gender, and patients with at least secondary level education . CONCLUSION: The prevalence of the use of CAM therapies is high in this study among people living with diabetes in Southwest, Nigeria. CAM is used mainly as complementary rather than as an alternative therapy. The most commonly used CAM therapy was biological therapy-Vernonia amygdalina (bitter leaf), garlic and Moringa oleifera. Orientation about CAM should be included in diabetes education at diagnosis and regularly re-enforced at subsequent clinic visits.

Feasibility of Hyperfunctioning Parathyroid Gland Localization Using [18F]fluciclovine PET/CT.

PURPOSE: To evaluate the ability of anti-1-amino-3-anti-1-amino-3-[18F]fluorocyclobutane-1-carboxylic acid ([18F]fluciclovine) positron emission tomography/X-ray computed tomography (PET/CT) in comparison to Technetium-99m 2-methoxy isobutyl isonitrile ([99mTc]sestamibi) single-photon emission computed tomography/CT (SPECT/CT) for the localization of hyperfunctioning parathyroid glands in patients with hyperparathyroidism. PROCEDURES: Four patients with hyperparathyroidism underwent 60-minutes sequential neck and thorax PET/CT after [18F]fluciclovine (352 ± 28 MBq) injection. Lesion uptake and target-to-background ratios (TBR) were compared with [99mTc]sestamibi (798 ± 27 MBq) SPECT/CT in the same patient. RESULTS: Both techniques detected 4/5 hyperfunctioning parathyroid glands identified at surgery. The highest [18F]fluciclovine uptake and TBRs were at 5-9 min with rapid washout. [99mTc]sestamibi had significantly higher TBRs compared with [18F]fluciclovine (5-9 min) for blood pool (10.9 ± 4.7 vs 1.3 ± 0.6; p < 0.01) and reference muscle backgrounds (5.8 ± 3.0 vs 1.7 ± 0.6; p < 0.01), with non-significant trend for thyroid tissue background (1.3 ± 0.5 vs 1.1 ± 0.5; p = 0.73). CONCLUSION: Hyperfunctioning parathyroid glands can be detected on [18F]fluciclovine PET/CT at early imaging, but conspicuity (TBR) is better with [99mTc]sestamibi. [18F]fluciclovine PET/CT does not seem promising in the detection of hyperfunctioning parathyroid glands.

Surgical decompression for traumatic spinal cord injury in a tertiary center.

BACKGROUND: There are controversies regarding the importance and timing of spinal cord decompression following trauma. Documented evidence shows that early decompression in the setting of acute spinal cord injury (SCI) improves neurologic outcomes. Our objective was to evaluate the outcome of posttraumatic spinal cord decompression with or without spinal stabilization in our region. METHODOLOGY: We performed a cross-sectional study on adult patients who presented with acute spinal cord compression of traumatic etiology within a 2-year period. The primary outcome was change in Frankel's grading 6 months after surgery. Secondary outcomes were complication rates and mortality. RESULTS: A total of 35 patients made up of 24 (68.6%) males and 11 (31.4%) females were recruited into the study. The spectrum of injuries included cervical 27 (77.1%), thoracic 7 (20.0%), and lumbar vertebrae 1 (2.9%). The outcome as measured by Frankel's grade at 6 months after surgery showed improvement in 9 (25.7%) patients following intervention. All patients who presented with Frankel's Grade C and D improved to Grade E while none of those who presented with Frankel's Grade E deteriorated. The common complications of spine decompression and fixation in this series were surgical site infections (11.4%) and chest infections (11.4%), especially in high cervical injury. CONCLUSION: Spinal cord decompression with spinal stabilization enhances the rehabilitation of patients with unstable spine and completes spinal cord injuries. Our experience shows improvement in neurological function in patients with spinal cord decompression despite the challenges of instrumentation in Sub-Saharan Africa.

Surgical site infection in posterior spine surgery.

BACKGROUND: Surgical site infections (SSIs) in spine surgery remain a significant cause of morbidity and prolonged hospitalization. Factors affecting SSI includes patient's comorbidities, duration of surgery, type and indication for surgery among others. We intend to document our experience in our center and highlight possible factors influencing SSI in posterior spine surgery. METHODOLOGY: All consecutive patients who had posterior spine surgeries between January 2012 and July 2014 were recruited into the study. All patients who had wound infection were noted and culture sensitivities were documented as well. Results were analysed to get the infection rate, reasons for prolonged stay on admission as well as possible contributing factors to wound infections. RESULTS: A total of 62 patients' records were reviewed with 34 males and 28 females (male:female = 1.2:1). SSI was classified as deep or superficial to the fascia. Ten (16.1%) patients were found to have an SSI with 7 (11.3%) patients having deep infections and 3 (4.8%) had superficial infection. Vertebral level operated, etiology, and diagnosis were not statistically significant for SSI. However, spinal instrumentation, surgery on cervical region and wound inspection on or before postoperative day 5 were associated with an increase in the rate of SSI. Comorbidities such as diabetes mellitus, obesity, and anemia were significant risk factors. The organisms cultured were Pseudomonas and Staphylococcus species. CONCLUSIONS: Wound infection is a significant complication of posterior spine surgery. This causes distress for both patient and surgeons alike. Uncontrolled diabetes, spine instrumentation and long duration of surgery are significant risk factors for SSI. Practices of early wound inspection, frequent wound dressing changes and not keeping to nontouch technique for changing and removing dressings are important risk factors for SSI in posterior spine surgeries that need to be changed to reduce the burden of SSI.

Improvised external ventricular drain in neurosurgery: A Nigerian tertiary hospital experience.

BACKGROUND: The most common type of hydrocephalus in developing countries is post infective hydrocephalus. Infected cerebrospinal fluid (CSF) however cannot be shunted for the reason that it will block the chamber of the ventriculo-peritoneal (VP) shunt due to its high protein content. In centers where standard external ventricular drain (EVD) sets are not available, improvised feeding tube can be used. AIM: The main focus of this study is to encourage the use of improvised feeding tube catheters for EVD when standard sets are not available to improve patients' survival. METHODOLOGY: This was a prospective study. Consecutive patients with hydrocephalus that cannot be shunted immediately for high chances of shunt failure or signs of increasing intracranial pressure were recruited into the study. Other inclusion criteria were preoperative brain tumor with possibility of blocked CSF pathway and massive intraventricular hemorrhage necessitating ventricular drainage as a salvage procedure. Standard EVD set is not readily available and too expensive for most of the parents to afford. Improvised feeding tube is used to drain/divert CSF using the standard documented procedure for EVD insertion. Outcome is measured and recorded. RESULTS: A total of 28 patients were recruited into the study over a time frame of 2 years. There were 19 (67.9%) male and 9 (32.1%) females with a ratio of about 2:1. Age ranges varied from as low as 7 days to 66 years. The median age of the study sample was 6.5 months while the mean was 173.8 months. Duration of EVD varied from 2 days to 11 days with a median of 7 while the average was 6 days. Eventual outcome following the procedure of EVD showed that 19 (67.9%) survived and were discharged either to go home or to have VP shunt afterwards while 8 (28.6%) of the patients died. CONCLUSIONS: External ventricular drain can and should be done when it is necessary. Potential mortalities could be reduced by the improvised drainage using a standard feeding tube as described.

Early decompressive craniectomy for traumatic brain injury in resource poor centres:a tertiary institution experience.

UNLABELLED: Decompressive craniectomy (DC) is the surgical management of increasing intracranial pressure (ICP) following a severe traumatic brain injury (TBI) as well as severe cerebrovascular insult. Established protocols for the use of DC include monitoring ICP and going through non-surgical methods to reduce ICP before DC which is the last resort. ICP monitors and facilities to render patients hypothermic and for inducing barbiturate coma are not readily available in resource depleted facilities, hence the need for a timely DC. DC when timely done saves lives and improves chances of survival following severe brain injury. AIMS AND OBJECTIVES: This study intends to justify early and appropriate DC in selected patients with radiological and clinical increasing ICP in resource poor centres. PATIENTS AND METHOD: A one-year prospective study of patients with severe brain injury with CT and clinical evidence of increasing ICP who had DC as the main modality of management. RESULTS: Ten patients were recruited into the study on the basis of deterioration in level of consciousness and CT evidence of raised intracranial pressure. Males were 8 (80%) and females were 2 (20%) with a ratio of 4:1. RTA accounted for 80% of aetiology of TBI. Out of the ten patients, 4 (40%) died after DC. Six (60%) of the patients survived and had cranioplasty with bone flap replacement (3), titanium (2) and acrylic (1). Two (20%) were discharged with GOS of 5, another 2 (20%) with 4 and last 2 (20%) with GOS of 3. CONCLUSION: Early decompressive craniectomy is beneficial for selected groups of patients most especially in settings where facilities for ICP monitoring and other medical options are not available.

Efficacy of endoscopic third ventriculostomy in the management of hydrocephalus in children under 2 years of age: experience from a tertiary institution in Nigeria.

BACKGROUND: The management of hydrocephalus in developing countries is challenging. Hydrocephalus is a common childhood disorder in developing countries in particular and its management is quite challenging. Ventriculoperitoneal (VP) shunt is associated with high failure rates and complications. Endoscopic third ventriculostomy (ETV) with potentially lower complication rate could improve care and reduce cost of management of hydrocephalus. OBJECTIVE: The aim of this study was to evaluate the efficacy (success rate) of ETV in children ≤2 years and to find out factors that may be responsible for good outcome of ETV. METHODS: This prospective observational study was conducted at Lagos University Teaching Hospital, Lagos. Nigeria. All consecutive children ≤2 years of age with hydrocephalus were recruited into the study. Relevant demographic and clinical data documented. All cases had ETV and were followed up to document 6 months outcome. RESULTS: A total of 34 patients (M: F ratio 1.1:1) were recruited over a 2-year period. Age, sex, presumed aetiology and image findings were not statistically significant in influencing outcome of ETV. Good outcome (defined as uneventful postoperative period, not requiring repeat ETV or VP shunt) was documented in 26 (73.5%). A total of 8 (26.5%) experienced poor outcome. Complication occurred in 2 (5.9%) as follows: Wound infection 1 (2.9%) and ventriculitis 1 (2.9%). Aetiology was divided into non post-infective hydrocephalus-20 (58.8%), post-infective hydrocephalus-5 (14.7%) and post-myelomeningocoele repair-9 (26.5%). CONCLUSIONS: This study shows that ETV success rate is high in the management of hydrocephalus in children ≤2 years in our clinical practice. Regardless of the clinical diagnosis, where the facilities are available, children with hydrocephalus will benefit from ETV irrespective of the age and aetiology in sub Saharan Africa.

Prophylactic effect of aqueous extract of Sesamum indicum seeds on ethanol-induced toxicity in male rats.

The liver is vulnerable to alcohol-related injury because it is the primary site of alcohol metabolism. Additionally, a number of potentially dangerous by-products are generated as alcohol is broken down in the liver. However, dietary supplements may prevent or relieve some of alcohol's deleterious effects. Therefore, this study was conducted to evaluate the prophylactic effect of aqueous extract of Sesamum indicum (SI) on ethanol induced toxicity in rats. Male Wistar albino rats were divided into control, ethanol, pre-treatment, simultaneous and post-treatment groups. In the prophylactic experiment, Sesamum indicum, (200 mg/kg body weight) was administered by oral gavage for 28 days; two hours before, simultaneously with or two hours after ethanol exposure. Toxicity was induced by administering 45% ethanol (4.8 g/kg bw) by oral gavage. Lipid peroxidation (TBARS) and reduced glutathione (GSH) levels and catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and gluthathione-S-transferase (GST) activities were then determined in the liver, serum triglyceride (TG) levels, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were monitored and histological examination was carried out. The results revealed that ethanol administration led to significant elevation of TBARS level while depleting in the level of GSH as well as CAT, GPx, SOD and GST activities. Similarly, TG level and ALT and AST activities were elevated. The SI pre-treated group significantly inhibited TBARS, restored GSH level, enhanced CAT, GPx, SOD and GST activities and significantly decreased the elevated level of serum TG, ALT and AST activities. SI treatment (simultaneously with ethanol) exhibited similar effects to those of the SI pre-treated groups, while the SI post-treated group did not show the same protection as the Pre-treated group. S. indicum possesses antioxidant and hepatoprotective properties, that eliminate the deleterious effects of toxic metabolites of ethanol.

Skinfold Parameter as Predictor of Body Density for Undergraduate Female Students in a Nigeria University Community

The purpose of this study was to determine if some selected skinfold parameters are visible in predicting body density (BD) of undergraduate female students of Obafemi Awolowo University; lie Ife.Methods: Seventy five non- athletic female students; age ranged from 18 to 30 years old from Obafemi Awolowo University (OAU) lie Ife; Nigeria participated in the study. Underwater weight was measured using Hydrostatic Weighing (HSW) equipment and skinfold was measured with Lange skinfold calipers using standard protocol at chest; biceps; triceps; forearm; mid axillar; subscapular; abdomen; supra illiac; thigh and lateral calf. Body density (BD) was calculated with underwater weight and skinfold measurement using some established equations. Descriptive statistics and Pearson Moment Correlation coefficient was used to analyze the data collected.Results: The result showed that BD by HSW had significant negative correlation with skinfold at chest (r = - .505; p .001); biceps (r

Choroid plexus papilloma in a Nigerian child: a case report.

Choroid plexus papilloma is a rare but known cause of hydrocephalus among children. We report the case of an 8 month old girl who clinically was thought to have post-infective Hydrocephalus. Cranial CT however showed an associated intra-ventricular tumour which after surgical resection was reported as a Choroid plexus papilloma. This is the first time such a case is being diagnosed at our hospital and very few cases have been documented in literature from sub-Saharan Africa. This case highlights the available treatment and good prognosis that is possible with Choroid plexus papilloma. It also highlights the need for a high index of suspicion for less common causes of an enlarging head especially when the clinical picture is not typical as demonstrated in our patient. The seeming rarity of this disease in our environment may be partly due to the limited availability of CT scanners in many centres and the paucity of Neuroradiologist and Neurosurgeons in sub-Saharan Africa.

The antimicrobial activities of processed Nigerian and South African black tea.

The antibacterial activity of processed Nigerian Lipton Tea and South African 5 Roses Tea, extracted using distilled water, chloroform and 70% ethanol were determined against nine (9) enteropathogenic bacteria which includes; Bacillus subtilis, Proteus sp, Enterobacter sp., Klebsiella pneumoniae, Escherichia coli, Salmonella typhi, Salmonella paratyphi A, Salmonella arizona and Staphylococcus aureus. The 2 tea bags, Nigerian Lipton tea bag and the South African 5 Roses tea bag were extracted through Solvent extraction method using 3 extraction solvents; Distilled water, Chloroform and 70% Ethanol and then inoculated onto the Mueller-Hinton agar plates containing the standard isolates at 6 different points on each plate. The zones of inhibition of the bacterial isolates produced by each of these tea - extracts were determined while meaningful antibacterial activities against five (5) of the standard isolates; Enterobacter sp., K. pneumoniae, S. paratyphi A, B. subtilis and S. aureus were observed. Lipton tea water-extract was a more effective antibacterial agent than water-extract of 5 Roses tea. While the 70% ethanol-extract of 5 Roses tea had the greatest antibacterial activity of all the different tea-extract used for the study, the chloroform-extracts of the tea bags had no antimicrobial effect on all the test organisms used.

Microbial and Chemical analysis of potable water in public­water supply within Lagos University, Ojo

Water samples were collected especially into sterile containers at four designated pints within the Lagos State University; Ojo Campus. The water samples were immediately subjected to both chemical and microbiological analysis in order to evaluate the quality of potable water in circulation within the university and identify its sources of contami- nation. Levels of iron; calcium and magnesium detectable in the circulating drinking water were far below the WHO recommended limits. However; more potentially dangerous discovery was the level of Coliform contamination which far exceeds the WHO standards. This explains the high incidence of water-borne diseases such as Dysentry; Diarrhea; Typhoid fever within the university population. Meanwhile; other microorganisms detected were E. coli; Pseudomonas aeruginosa; Staphylococcus aureus; yeasts and moulds. The pH of potable water in circulation falls within recommended limits (6.0-8.0) but for faculty of arts and social sciences that had pH of 5.5 which suggested a high degree of public health concern. There is the need for adequate changes to be made at points where water distribution systems integrity appeared compromised. The university community is advised to boil water before drinking in order to avoid consumption of unwholesome biological agents in the water distribution

Outpatient interval female sterilization at the University College Hospital, Ibadan, Nigeria.

Between September 1975 and April 1981, 258 patients were sterilized at the University College Hospital (UCH), Ibadan, using minilaparotomy and laparoscopy. Selected sociodemographic data as well as the technical and surgical difficulties encountered have been reviewed in this article. The mean age at sterilization was 38.3 years whilst the mean parity was 7.2. In all, there were 215 minilaparotomy sterilizations and forty-three laparoscopic sterilizations. Surgical difficulties were reported for 12.1% of minilaparotomy and 11.6% for laparoscopic procedures. The most frequently reported difficulties were obesity and pelvic adhesions. The failure rates were 1.4% for minilaparotomy and 2.3% for laparoscopy.

PIP: This study reviewed selected sociodemographic data as well as technical difficulties encountered in the 258 outpatient female sterilization procedures performed at the University College Hospital in Ibadan, Nigeria, in 1975-81. This series included 215 minilaparotomy sterilizations and 43 laparoscopic procedures. Only women who requested sterilization for family planning reasons were include in the study. The mean age at sterilization was 38.3 years. 91% of women had 5 or more living children, with a mean parity of 7.2. 52% of sterilization patients had no education and only 8% had 13 or more years of schooling. Surgical difficulties were encountered in 12% of minilaparotomy and 12% of laparoscopic procedures. The most frequently reported problems were obesity and adhesions. Postoperative complaints were minimal, but generally involved abdominal pain. The failure rates were 1.4% for minilaparotomy and 2.3% for laparoscopy. These findings suggest that the target population for female sterilization in Nigeria would be multiparous women with 5 or more deliveries aged 35 years and above. It appears that the introduction of interval outpatient sterilization and demonstration and the demonstration of its safety is gradually leading to increased acceptance of sterilization as a method of family limitation.

Plasma high-density-lipoprotein cholesterol levels during long-term use of an oral contraceptive in Nigerian women.

Total and high-density lipoprotein (HDL) cholesterol were estimated in 131 blood samples obtained from women who had been taking the oral contraceptive Noriday 1 + 50Fe (one packet contains 21 tablets of 1 mg of norethindrone + 0.05 mg of mestranol, and 7 of 75 mg of ferrous fumarate) for 1-60 months. Thirty five women who had never used oral contraception (OC) formed the control group. There was a significantly higher mean HDL cholesterol level, and HDL cholesterol/total cholesterol ratio but not total cholesterol level in the women who had been using OC for 19-60 months. The values in women who had been using OC for 1-18 months were not significantly different from those in the control group. The increase in the HDL cholesterol level may not depend on the oestrogen content of the oral contraceptive but on the duration of its intake.

PIP: Total and high-density lipoprotein (HDL) cholesterol were estimated in 131 blood samples obtained from women who had been taking the oral contraceptive (OC) Noriday 1+50Fe (1 packet contains 21 tablets of 1 mg of norethindrone + 0.05 mg of mestranol, and 7 of 75 mg of ferrous fumarate) for 1-60 months. 35 women who had never used OCs formed the control group. There was a significantly higher mean HDL cholesterol level and HDL cholesterol/total cholesterol ratio but not total cholesterol level in the women who had been using OCs for 19-60 months. Values in women who had been using OCs for 1-18 months were not significantly different from the control group. The increase in the HDL cholesterol level may not depend on the estrogen content of the OC but on the duration of its intake. This increase may be beneficial as HDL-cholesterol has been reported to have an anti-atherogenic effect.