RESUMO
During the search for secondary metabolites with antiproliferative activity, six new lanostane triterpenoids, tyrosamic acids A-F (1-6) together with ten known compounds (7-16), were isolated from the fruiting body of Tyromyces sambuceus. Their structures were elucidated using MS analyses, extensive 2D-heteronuclear NMR data interpretation and the structure of 3 was further confirmed by single-crystal X-ray data analyses. All lanostane triterpenoids (1-16) possesses a carboxy group at C-20 position and their strength of antiproliferative activity was affected by the presence or absence of a hydroxy group at C-15 position and at the side chain. Four of the compounds (1, 6, 10, 14) showed antiproliferative activities against human cancer cell lines with IC50 values of 16.8-48.3 µM (HL-60).
Assuntos
Antineoplásicos/farmacologia , Carpóforos/química , Polyporaceae/química , Triterpenos/química , Antineoplásicos/química , Linhagem Celular Tumoral , Humanos , Triterpenos/classificaçãoRESUMO
Three new lanostane triterpenoids (1, 7, 8) and five new lanostane triterpene glucosides (2-6) have been isolated from the fruit bodies of Daedalea dickinsii. Their structures were established primarily by NMR experiments, and their biological activity against HL-60 and HCT-15 cell lines was investigated. Compounds 3-6 induced internucleosomal DNA fragmentation characteristic of apoptotic cell death in the HL-60 cell line.