[The classification of ileostomies]. / Klassifikatsiia ileostom.

From experience in the treatment of 527 patients the authors suggested an original classification of ileostomy. The systematization was based on the following criteria: the object of ileostomy, the shape and method for formation of the opening, the peculiarities of its construction, localization, functions, as well as the nature of complications linked with ileostomy. The suggested classification takes into account the modern concept on artificially formed intestinal fistulas, and gives a complete idea of patients with ileostomy. The use of the principles of the suggested classification in practice allows a rational variant of ileostomy to be chosen according to the existing conditions and effective measures for rehabilitation of patients with ileostomy to be marked out later.

[Cloning of the locus encoding synthesis of an outer membrane protein (Omp2) of the calcium dependence plasmid of Yersinia pestis (Lehmann, Neumann)]. / Klonirovanie lokusa plazmidy kal'tsiizavisimosti Yersinia pestis (Lehmann, Neumann), kodiruiushchego sintez belka vneshnei membranyi (BVM2)

The genetic locus of Yersinia pestis encoding synthesis of a 46-kDa heat-inducible outer membrane protein (Omp2) was cloned into pBR322 plasmid. The Omp2 was shown to be analogous to previously described YopH and Yop2b proteins. The fifth HindIII fragment of 48-MDa calcium dependence plasmid pCad358 mediates production of 31- and 28-kDa proteins, irrespective of orientation of the insertion. A 31-kDa polypeptide seems to correspond to the YopJ described elsewhere. The maps of BamHI and HindIII of pCad358 region studied differed from those described for pCD1 plasmid of Y. pestis KIM. The products encoded by genes from the fragment cloned in the Pgm+ background give rise to considerable growth of Y. pestis within mouse peritoneal macrophages but were not sufficient to cause lethal infectious process.

[The role of IS-elements of Yersinia pestis (Lehmann, Neumann) in the emergence of calcium-independent mutations]. / Rol' IS-élementov Yersinia pestis (Lehmann, Neumann) v vozniknovenii mutatsii kal'tsiinezavisimosti.

Calcium independent mutants of two Yersinia pestis strains were studied. Insertions of IS100 element at three different sites of plasmid pCad within calcium dependence region were detected in Y. pestis EV, as well as two extensive deletions covering the whole region. It was shown that IS100 carries no HindIII sites. Novel IS element of Y. pestis designated IS101 was discovered in strain 358, in addition to IS100. It is distinguished by a slightly smaller size, HindIII site presence and high specificity of integration.

[Expression of Yersinia pestis antigens encoded by the Ca2+-dependence plasmid]. / Ekspressiia antigenov chumnogo mikroba, kodiruemykh plazmidoi Ca2+-zavisimosti.

Antigens coded by the Ca2(+)-dependance plasmid were found in the cultural medium, cytoplasm and outer membranes of the three monoplasmid (pCadV) strains of Yersinia pestis with the different basic properties. The presence of 20 mM of Mg2+ at least in the medium is necessary for optimal expression of these proteins. The existence of strain differences in the bacterial cells reaction to temperature, cultivation medium has been demonstrated. No difference in the pCad-dependent proteins was found in Yersinia pestis and the causative agents of pseudotuberculosis, enterocolitis.

[Reconstructive surgery in patients with nonspecific ulcerative colitis and Crohn disease]. / Rekonstruktivnye operatsii u bol'nykh nespetsificheskim iazvennym kolitom i bolen'iu Krona.

The article analyses experience in reconstructive operations on 89 patients with unspecific ulcerative colitis and Crohn's disease in the period from 1960 to 1987. Most frequently, surgical rehabilitation of these patients proved to be possible when the time between the onset of the disease and the primary operation was no more than 3 years. Special selection of patients for reconstructive operations does not exclude those with complications in the anorectal region, the manifestations of which should be, however, minimal. The inflammatory process in the rectum should not exceed the first degree of activity. The most favourable periods for reconstructive operations are 6 to 12 months after removal of the colon. The best results were achieved in patients with ileorectal and a low ileosigmoid anastomosis which were created in 90% of patients with unspecific ulcerative colitis and in 41% of those with Crohn's disease.

[Functional state of the distal part of the large intestine in Crohn disease]. / Funktsiona'lnoe sostoianie dista'lnogo otdela tolstoi kishki pri bolezni Krona.

State of the locking apparatus has been studied in 22 patients with Crohn's disease free of the anorectal complications. Even prior to the development of these complications caused by metabolic abnormalities and inflammatory changes in the intestinal wall the locking apparatus undergoes certain functional changes.