Resección tarsal vertical limitada en un caso de carcinoma basocelular de párpado inferior / Limited vertical tarsal resection in a case of basal cell carcinoma of the lower eyelid

Exponemos el caso clínico de una mujer de 71 años de edad con antecedente de múltiples carcinomas basocelulares (CBC) que presenta una lesión nodular en la totalidad de la extensión del borde libre de párpado inferior. Se realiza un abordaje de la lesión mediante exéresis del margen palpebral con resección limitada vertical de tarso y colgajo de Tripier con un resultado estético y funcional correcto, márgenes histológicos libres y ausencia de recidiva en un seguimiento de 12 meses (AU)

We present the clinical case of a 71-year-old woman with a history of multiple basal cell carcinomas (BCC) who presented a nodular lesion in practically the entire extension of the free edge of the lower eyelid. The lesion was approached by excision of the palpebral margin with limited vertical resection of the tarsus and Tripier flap with a correct aesthetic and functional result, free histological margins and no recurrence in a 12-month follow-up (AU)

[Comparison between the standard MPN method recommended by the American Public Health Association and the Simplate and Petrifilm methods for identification of the coliform group and Escherichia coli in tilapia (Oreochromis sp) originating from aquaculture in fresh water]. / Comparación entre el método estándar sugerido por APHA y los métodos Simplate y Petrifilm, para la identificación del grupo coliforme y de Escherichia coli en tilapia (Oreochromis sp) procedente de piscicultura de agua dulce.

We have compared three methods for the detection and quantification of coliforms and E. coli in fish: the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapia's external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1%, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100% of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.

Comparación entre el método estándar sugerido por APHA y los métodos Simplate y Petrifilm, para la identificación del grupo coliforme y de Escherichia coli en tilapia (Oreochromis sp) procedente de piscicultura de agua dulce / Comparison between the standard MPN method recommended by the American Public Health Association and the Simplate and Petrifilm methods for identification of the coliform group and Escherichia coli in tilapia (Oreochromis sp) originating from aquaculture in fresh water

We have compared three methods for the detection and quantification of coliforms and E. coli in fish: the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapia's external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100 of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.

Comparación entre el método estándar sugerido por APHA y los métodos Simplate y Petrifilm, para la identificación del grupo coliforme y de Escherichia coli en tilapia (Oreochromis sp) procedente de piscicultura de agua dulce / Comparison between the standard MPN method recommended by the American Public Health Association and the Simplate and Petrifilm methods for identification of the coliform group and Escherichia coli in tilapia (Oreochromis sp) originating from aquaculture in fresh water

We have compared three methods for the detection and quantification of coliforms and E. coli in fish: the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapias external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100 of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.(AU)

Comparación entre el método estándar sugerido por APHA y los métodos Simplate y Petrifilm, para la identificación del grupo coliforme y de Escherichia coli en tilapia (Oreochromis sp) procedente de piscicultura de agua dulce. / [Comparison between the standard MPN method recommended by the American Public Health Association and the Simplate and Petrifilm methods for identification of the coliform group and Escherichia coli in tilapia (Oreochromis sp) originating from aquaculture in fresh water]

We have compared three methods for the detection and quantification of coliforms and E. coli in fish: the conventional three-tube MNP method (APHA), the Simplate Method and the Petrifilm Method. Thirty samples of the tilapias external surface (skin, gills and fin) produced in an artificial pond were collected. Samples were processed individually in buffered peptone water 1

, then they were diluted and inoculated in Simplate, Petrifilm and LST tubes. Coliforms were isolated from 100

of the samples. The Simplate and 3M Petrifilm results were obtained after 24 h of incubation. The conventional three-tube MNP method showed results only after 96 hours of incubation. Simplate showed better recovery of coliforms and E. coli than 3 M Petrifilm and APHA method (P < 0.05). 3M Petrifilm and APHA were not significantly different for E. coli recovery.