Transcriptome analyses suggest that changes in fungal endophyte lifestyle could be involved in grapevine bud necrosis.

Bud necrosis (BN) is a common disorder that affects Vitis vinifera L. and reduces its potential yield. To minimize the losses caused by BN, the double pruning management was applied in Brazilian Southeast vineyards. In this management strategy plants are pruned at the winter to promote a vegetative cycle and then, at summer, to promote the reproductive cycle at optimal environmental conditions. To investigate the relationship of BN and the double pruning management RNA-seq libraries were sequenced from healthy and necrotic tissues at four different stages of the year. The comparison of differentially expressed genes in necrotic and non-necrotic tissues showed an enhanced expression of genes related to cell death possibly induced by endophytic microorganisms in the necrotic tissues. The de novo assembly, characterization and quantification of transcripts within the RNA-seq libraries showed that genes from the endophytic fungus Alternaria alternata, responsible for the production of toxic compounds were highly expressed under BN. Here we propose a model in which unfavorable conditions and reduced carbohydrate levels in buds can promote the switch from a biotrophic lifestyle to a necrotrophic lifestyle in the endophytic fungi, which seems to be involved in the development of BN.

Índice s no diagnóstico da qualidade estrutural de latossolo muito argiloso sob manejo intensivo / S index in structural quality diagnostic of a very clayey oxisol under intense management

Neste estudo foi verificada a adequação de uso do índice S no diagnóstico da qualidade estrutural de um Latossolo Vermelho distrófico de cerrado cultivado com cafeeiro sob sistema de manejo que adota a aplicação de altas doses de gesso na linha da cultura, cultivo e manejo mecanizado de braquiária na entrelinha da cultura, além de preparo do sulco de plantio até 60 cm de profundidade, dentre outras técnicas de manejo intensivo. O índice S foi correlacionado à densidade e à porosidade do solo nas profundidades de 5, 20, 40 e 80 cm, na linha do cafeeiro, e 5, 10, 20, 40, 65 cm na entrelinha do cafeeiro. Para a determinação do índice S nas diferentes profundidades e locais de amostragem foram construídas curvas de retenção de água, sendo utilizadas amostras com estrutura preservada. Os valores deste atributo físico foram superiores a 0,045 indicando ótima qualidade física do solo sob o sistema de manejo adotado. Houve boa correlação entre o índice S com os atributos físicos analisados, mostrando ser esta uma boa ferramenta a ser utilizada no diagnóstico da qualidade estrutural do solo.

In this study we verified the adequacy of use of the Dexter S index in the diagnosis of structural quality of a Cerrado Oxisol cultivated with coffee in management system that adopts the application of high doses of gypsum in row culture, mechanized cultivation and management of Brachiaria in the interrows, and preparation of the furrow 60 cm deep, among other techniques of intensive management. The S was correlated with the density and porosity of the soil at depths of 5, 20, 40 and 80 cm along the row of coffee, and 5, 10, 20, 40, 65 cm between rows of coffee. For the determination of the S at different depths and sampling sites were built water retention curves, using samples with structure preserved. The values of this physical attribute were greater than 0.045 indicating good soil physical quality under the management system adopted. There was good correlation between the S with the physical attributes analyzed, showing that this is a good tool to use in the diagnosis of structural soil quality.