UCEasy: A software package for automating and simplifying the analysis of ultraconserved elements (UCEs).

BACKGROUND: The use of Ultraconserved Elements (UCEs) as genetic markers in phylogenomics has become popular and has provided promising results. Although UCE data can be easily obtained from targeted enriched sequencing, the protocol for in silico analysis of UCEs consist of the execution of heterogeneous and complex tools, a challenge for scientists without training in bioinformatics. Developing tools with the adoption of best practices in research software can lessen this problem by improving the execution of computational experiments, thus promoting better reproducibility. NEW INFORMATION: We present UCEasy, an easy-to-install and easy-to-use software package with a simple command line interface that facilitates the computational analysis of UCEs from sequencing samples, following the best practices of research software. UCEasy is a wrapper that standardises, automates and simplifies the quality control of raw reads, assembly and extraction and alignment of UCEs, generating at the end a data matrix with different levels of completeness that can be used to infer phylogenetic trees. We demonstrate the functionalities of UCEasy by reproducing the published results of phylogenomic studies of the bird genus Turdus (Aves) and of Adephaga families (Coleoptera) containing genomic datasets to efficiently extract UCEs.

Validity and reliability of a 6-a-side small-sided game as an indicator of match-related physical performance in elite youth Brazilian soccer players.

The aims of this study were: (i) to compare the external and internal load during a 6-a-side small-sided game (6v6-SSG) according to age-group; (ii) to relate these parameters between the 6v6-SSG and official matches; and (iii) to test the reliability of the 6v6-SSG. A total of 51 Brazilian youth soccer players participated in this study (U11 [n = 16]; U13 [n = 10]; U15 [n = 9]; U17 [n = 8]; U20 [n = 8]). Three experiments were conducted. Experiment A: fifty-one U11 to U20 players were submitted to 6v6-SSGs (n = 10 games; two for each age-group). Experiment B: thirty-two players were randomized to also play official matches (n = 6 matches). Experiment C: thirty-five youth players played the 6v6-SSG twice for test and retest reliability analysis. External load was obtained using Global Positioning Systems and the internal load parameter was calculated through mean heart rate. Statistical approaches showed progressive increases in all parameters according to categories (U11< U13< U15< U17< U20; p < 0.05; ES = 0.42-23.68). Even controlling for chronological age, all parameters showed likely to almost certain correlations between 6v6-SSG and official matches (r = 0.25-0.92). Collectively, the proposed protocol indicates good reliability (CV% = 2.0-12.6; TE% = 2.3-2.7%; ICC = 0.78-0.90). This research suggests that the 6v6-SSG is an alternative tool to indicate match-related physical performance in youth soccer players.

Team Dynamics, Running, and Skill-Related Performances of Brazilian U11 to Professional Soccer Players During Official Matches.

Palucci Vieira, LH, Aquino, R, Moura, FA, Barros, RMLd, Arpini, VM, Oliveira, LdP, Bedo, BLdS, and Pereira Santiago, PR. Team dynamics, running, and skill-related performances of Brazilian U11 to professional soccer players during official matches. J Strength Cond Res 33(8): 2202-2216, 2019-Analyses of movements during soccer competition have been used previously to help develop conditioning programs. However, this has not been extensively studied in youth populations. Therefore, the purposes of this study were to examine (1) dynamics of collective tactical movements, (2) running, and (3) skill-related performances during soccer matches disputed by children to senior players. A total of 120 Brazilian players in the age groups U11, U13, U15, U17, U20, and professional (PRO) were monitored during official competition matches (N = 12). Using semiautomatic video-based tracking (30 Hz), match running variables including total distance traveled, average speed, maximum sprint speed, and high-intensity activities were evaluated. Tactical metrics were computed as team surface area, spread, and median frequency. Through notational analysis, technical skills such as involvements with the ball, passes, ball touches, duels, and goal attempts were also recorded. One-way analysis of variance and magnitude-based inferences were used to detect differences between ages. Although the average speed, team surface area, and spread tended to present stabilized increases from the U15 (e.g., U15 > U13 > U11), maximal sprinting speed (PRO > U17 > U15, U13, U11) and percentage at very high-intensity activities (U20 > PRO, U17 > U15 > U13 > U11) demonstrated continuous gains. Median frequencies were higher in the younger groups (U13, U15, U17 > U20, PRO), although the percentage of successful passes was higher in the older groups (PRO > U17, U15 > U13, U11). We concluded that Brazilian U11 to PRO players present different performance profiles for running, collective movement dynamics, and technical skills, and that the rate of development regarding these components varies. Coaches should be aware of these differences to select and adapt training content for each age group.

Acute Effects of Active, Ballistic, Passive, and Proprioceptive Neuromuscular Facilitation Stretching on Sprint and Vertical Jump Performance in Trained Young Soccer Players.

Oliveira, LP, Vieira, LHP, Aquino, R, Manechini, JPV, Santiago, PRP, and Puggina, EF. Acute effects of active, ballistic, passive, and proprioceptive neuromuscular facilitation stretching on sprint and vertical jump performance in trained young soccer players. J Strength Cond Res 32(8): 2199-2208, 2018-The aim of this study was to compare the acute effects of active (AC), ballistic (BA), passive (PA), and proprioceptive neuromuscular facilitation (PNF) stretching methods on performance in vertical jumping, sit and reach, and sprinting in young soccer players. Twelve trained soccer players (17.67 ± 0.87 years) participated in the study. The jump height (H), peak power (PP), and relative power (RP) in the squat jump (SJ) and countermovement jump (CMJ), the range of motion (ROM), the rating of perceived exertion (RPE), and time (seconds) in 10-20-30-m sprints were evaluated. Significant differences (p < 0.05) in H were found in the comparisons between the PA and control (CO) condition for the SJ. For the CMJ, differences in H were observed between the PA and CO, and PNF with CO and BA, and in the PP between the PNF and CO, AC, and BA, as well as in the RP between the PNF and BA. Significant increases in ROM were found in the AC, BA, PA, and PNF compared with the CO. In relation to RPE, higher scores were reported in the PA and PNF conditions compared with the AC and BA. No significant differences were found in 10-20-30-m sprints. Therefore, the AC and BA methods can be used before vertical jump and sprint activities, with the aim of increasing flexibility. However, the PA and PNF methods should be avoided because of subsequent negative effects on vertical jump performance.

Periodization Training Focused on Technical-Tactical Ability in Young Soccer Players Positively Affects Biochemical Markers and Game Performance.

Aquino, RLQT, Cruz Gonçalves, LG, Palucci Vieira, LH, Oliveira, LP, Alves, GF, Pereira Santiago, PR, and Puggina, EF. Periodization training focused on technical-tactical ability in young soccer players positively affects biochemical markers and game performance. J Strength Cond Res 30(10): 2723-2732, 2016-The aim of this study was to investigate the effects of 22 weeks of periodized training, with an emphasis on technical-tactical ability, on indirect markers of muscle damage, and the on-field performance of young soccer players. Fifteen players (age 15.4 ± 0.2 years, height 172.8 ± 3.6 cm; body mass 61.9 ± 2.9 kg; % fat 11.7 ± 1.6; V[Combining Dot Above]O2max 48.67 ± 3.24 ml·kg·min) underwent 4 stages of evaluation: prepreparatory stage-T0; postpreparatory stage-T1; postcompetitive stage I-T2 and; postcompetitive stage II-T3. The plasmatic activity of creatine kinase (CK) and lactate dehydrogenase (LDH) were evaluated, as well as the on-field performance (movement patterns, tactical variables). Regarding the plasmatic activity of CK and LDH, there was a significant reduction (p ≤ 0.05) throughout the periodization training (T0: 350 U·L; T3: 150 U·L). Significant increases were observed (p ≤ 0.05) in the intensity of the game, high-intensity activities (HIA) (T0: 22%; T3: 27%), maximum speed (T0: 30 km·h; T3: 34 km·h) and tactical performance, team surface area (T0: 515 m; T3: 683 m), and spread (T0: 130 m; T3: 148 m). In addition, we found significant inverse correlations between the percentage variation of T0 to T3 in CK and LDH activities with percentage variation in high-intensity running (r = -0.85; p ≤ 0.05 and r = -0.84; p < 0.01, respectively) and HIA (r = -0.71 and r = -0.70; p ≤ 0.05, respectively) during the matches. We concluded that there was reduced activity in biochemical markers related to muscle damage, as well as increases in-game high-intensity performance and the tactical performance of the study participants. Furthermore, players who showed greater reduction in plasma activity of CK and LDH also obtained greater increases in-game high-intensity performance along the periodization. These results may contribute to the expansion and future consolidation of the knowledge of coaches and sport scientists to develop effective methodologies for training in soccer.

Biochemical, physical and tactical analysis of a simulated game in young soccer players.

BACKGROUND: The objectives of this study were to describe and compare the displacement patterns and the tactical performance of the players in the first to the second game time and verify possible associations between indirect markers of muscle damage with displacement patterns in a simulated game played by young soccer players. METHODS: Eighteen young soccer players were submitted to a simulated game and two blood collections, one before and another 30 minutes post-game to analyze the behavior of creatine kinase and lactate dehydrogenase enzymes. The patterns of displacement and tactics variables were obtained through functions developed in MATLAB environment (MathWorks, Inc., Natick, MA, USA). RESULTS: It is observed a significant increase in average speed (P=0.05), number of sprints (P<0.001), the percentage the total distance covered at high intensity (P<0.001) and tactical variables (team surface area: P=0.002; spreading: P=0.001) in the second period of the simulated game. In addition, there was significant reduction in the percentage of the total distance at low intensity (P≤0.05) in the second period, and there was a strong association between the percentage of change delta of creatine kinase and lactate dehydrogenase with the displacement patterns in the simulated game. CONCLUSIONS: The results show that indirect markers of muscle damage have great association with displacement patterns in game performed in training conditions for young soccer players, evidencing a need for reflection on the post-training recovery sessions strategies, contributing to better planning of sessions throughout the macrocycle.