1.
Nat Biotechnol
; 41(12): 1729-1733, 2023 Dec.
Artigo
em Inglês
| MEDLINE
| ID: mdl-36879007
RESUMO
Spinal cord circuits play crucial roles in transmitting pain, but the underlying activity patterns within and across spinal segments in behaving mice have remained elusive. We developed a wearable widefield macroscope with a 7.9-mm2 field of view, ~3- to 4-µm lateral resolution, 2.7-mm working distance and <10-g overall weight and show that highly localized painful mechanical stimuli evoke widespread, coordinated astrocyte excitation across multiple spinal segments.