Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy.

The next milestone of synthetic biology research relies on the development of customized microbes for specific industrial purposes. Metabolic pathways of an organism, for example, depict its chemical repertoire and its genetic makeup. If genes controlling such pathways can be identified, scientists can decide to enhance or rewrite them for different purposes depending on the organism and the desired metabolites. The lignocellulosic biorefinery has achieved good progress over the past few years with potential impact on global bioeconomy. This principle aims to produce different bio-based products like biochemical(s) or biofuel(s) from plant biomass under microbial actions. Meanwhile, yeasts have proven very useful for different biotechnological applications. Hence, their potentials in genetic/metabolic engineering can be fully explored for lignocellulosic biorefineries. For instance, the secretion of enzymes above the natural limit (aided by genetic engineering) would speed-up the down-line processes in lignocellulosic biorefineries and the cost. Thus, the next milestone would greatly require the development of synthetic yeasts with much more efficient metabolic capacities to achieve basic requirements for particular biorefinery. This review gave comprehensive overview of lignocellulosic biomaterials and their importance in bioeconomy. Many researchers have demonstrated the engineering of several ligninolytic enzymes in heterologous yeast hosts. However, there are still many factors needing to be well understood like the secretion time, titter value, thermal stability, pH tolerance, and reactivity of the recombinant enzymes. Here, we give a detailed account of the potentials of engineered yeasts being discussed, as well as the constraints associated with their development and applications.

Metabolic pathways of an organism depict its chemical repertoire and its genetic makeup.Autonomous synthetic microbes can be developed for lignocellulose biorefinery (LCB).LCBs can be harnessed with synthetic microbes to boost global bioeconomy.Yeasts can be engineered to enhance downstream process of LCB.

Shotgun metagenomics dataset of Striga hermonthica-infested maize (Zea mays L.) rhizospheric soil microbiome.

This dataset includes shotgun metagenomics sequencing of the rhizosphere microbiome of maize infested with Striga hermonthica from Mbuzini, South Africa, and Eruwa, Nigeria. The sequences were used for microbial taxonomic classification and functional categories in the infested maize rhizosphere. High throughput sequencing of the complete microbial community's DNA was performed using the Illumina NovaSeq 6000 technology. The average base pair count of the sequences were 5,353,206 bp with G+C content of 67%. The raw sequence data used for analysis is available in NCBI under the BioProject accession numbers PRJNA888840 and PRJNA889583. The taxonomic analysis was performed using Metagenomic Rapid Annotations using Subsystems Technology (MG-RAST). Bacteria had the highest taxonomic representation (98.8%), followed by eukaryotes (0.56%), and archaea (0.45%). This metagenome dataset provide valuable information on microbial communities associated with Striga-infested maize rhizosphere and their functionality. It can also be used for further studies on application of microbial resources for sustainable crop production in this region.

Corrigendum to "Shotgun metagenomics dataset of Striga hermonthica-infested maize (Zea mays L.) rhizospheric soil microbiome" [Data in Brief, volume 48 (2023) 1-5/109132].

[This corrects the article DOI: 10.1016/j.dib.2023.109132.].

Characterization and antagonistic potentials of selected rhizosphere Trichoderma species against some Fusarium species.

Trichoderma fungi have been proved as efficient bioagents with great antifungal properties while many species in the plant's rhizospheres have been characterized as plant growth-promoting agents. However, many rhizosphere Trichoderma are yet to be fully explored for plant disease management. In this study, Trichoderma species were isolated from the rhizosphere of maize, banana, and cassava, and their biocontrol potentials were screened against some Fusarium species from oak leaves (F2B and F3) and laboratory cultures (Fus 296 and Fus 294). The isolated rhizosphere Trichoderma were identified as Trichoderma virens 1 (TCIV), T. virens 2 (TCVII), T. virens 3 (TMSI), T. hazianum strain 1 (TCVI), T. harzianum strain 2 (TCVIII), T. erinaceum (TMZI), and T. koningiopsis (TMZII). The dual culture experiment recorded the highest percentage inhibition in TMZII against OakF2B (31.17%), TCVIII against Fus 294 (45.18%), TMZI against Fus 296 (47.37%), while TCIV was most effective against Oak F3 (44.15%). Among the Trichoderma culture filtrates evaluated, TCIV showed the highest percentage inhibition against Oak F3 (52.39%), Oak F2B (48.54%), Fus 294 (46.65%), and Fus 296 (44.48%). All the Trichoderma isolates demonstrated expressed varying levels of antagonism against the Fusarium pathogens in vitro.

Trichoderma: Potential bio-resource for the management of tomato root rot diseases in Africa.

Trichoderma spp. are among the front-line microorganisms commonly employed in novel biotechnology applications. They have been well-proven as biopesticides, biofertilizers, and biostimulants for managing plants against biotic and abiotic stresses. They are instrumental in managing plant diseases of economic importance, such as tomato root rot. However, this group of fungi has not been well-exploited en-mass in developing countries, while the use of bioagents in-lieu of chemical pesticides is still not a common practice in many African countries. Africa contributes 11.8% to global tomato production. Unfortunately, more than half of the actual product is lost due to diseases. The root rot of tomatoes predominantly caused by soil-borne fungal pathogens are among significant problems of tomato cultivation in Africa. Here, we review the constraints of tomato root rot in Africa and the roles of Trichoderma in repositioning the crop for optimum productivity. We gave a comprehensive overview of the economic importance, root rot epidemiology, and how to circumvent it through gene pool to resistant tomato and employ Trichoderma's biological control potentials. Furthermore, this review gives an overview of the mechanisms of action of Trichoderma, gaps in the advocacy, adoption, commercialization, and regulation of Trichoderma as biocontrol agents of tomato rot diseases in Africa.

Phytoremediation technology and food security impacts of heavy metal contaminated soils: A review of literature.

Heavy metal accumulation in soil and water is one of major problems caused by inorganic contaminants. Their presence in agricultural soils in high quantities have impacted the food security significantly and, by extension, the human health. Amongst various physico-chemical methods available for remediation of heavy-metals-polluted-sites, phytoremediation approaches have been found to be safe and environment friendly. This review gathered scattered information on heavy metal phytoremediation studies published in both review and research articles. It described the impact of heavy metals on food security and comprehensively discussed the application of different phytoremediation approaches for treatment of heavy metal-polluted soils, the basic principles underlining them, their strengths and weaknesses. Our findings indicated that, while hundreds of hyper-accumulator plants are being reported yearly, only few describe limitations inherent in them, such as low growth rate, low biomass production, and low metal tolerance. Hence, this review also gave a detailed overview of research gaps in phytotechnology and advocates consideration of the 'omics' studies; genomics, proteomics, metabolomics and likes in selecting and enhancing potential plants for phytoremediation. For a sustainable large-scale phytoremediation application, we established a multi-technology repair strategy via the combination of different methods like application of biological composts, plant-growth promoting microorganisms, and phytohormones for stimulation of the plant-growth during phytoremediation. We also gave comprehensive insights to proper disposal of plants used for phytoremediation, this subject is often not well considered/planned while deciding the application of plants for removal of heavy metals from polluted environments.