[Clinical and experimental aspects of the treatment effect of laser irradiation].

The article summarizes literature data and results of the authors' studies on clinical experimental aspects of the treatment effect of laser emission. The literature shows that among different therapeutic effects of laser emission, the most studied are neurotropic, methabolic, immunomodulating, antioxidant, anti-inflammatory as well as its ability to stimulate the regeneration of wounds and bones. The authors stressed on biostimulative effect of laser therapy.

[Preparation of diagnostic sera to enteroviruses 69, 70 and 71 having medical importance]. / Prigotovlenie diagnosticheskikh syvorotok k énterovirusam 69, 70 i 71, imeiushchim meditsinskoe znachenie.

The use of enteroviruses, types 69, 70 and 71, purified with freon and concentrated with polyethylene glycol, for immunizing rabbits, following 4 immunization schedules, has made it possible to obtain active virus-neutralizing diagnostic sera with antibody titers of up to 1:2560, 1:806 and 1:9123, respectively.

[Participation of Coxsackie B viruses in the occurrence and recurrence of infectious-allergic myocarditis (a serological study)]. / Uchatsie virusov Koksaki B v vozniknovenii i retsidivirovanii infektsionno-allergicheskogo miokardita (serologicheskoe issledovanie).

The serological study of sera from patients with infectious allergic myocarditis and from healthy persons has revealed essential differences in the occurrence and titers of antibodies to Coxsackie virus B. In patients with infectious allergic myocarditis the infectious process has been found to be significantly more frequently linked with Coxsackie viruses B, serovars 2 and 4.

[Detection of antibodies against enterovirus 71 in the sera of Moscow residents]. / Vyiavlenie antitel protiv énterovirusa 71 v syvorotkakh zhitelei Moskvy.

Twenty three out of 55 serum samples obtained from subjects aged 20-55 years have proved to be seropositive, 15 of the positive serum samples (i.e. more than 50%) possessing the titer greater than or equal to 1:16. The presence of specific antibodies in the sera of the inhabitants of Moscow suggests that either enterovirus 71 or some other virus whose antigens are partly identical to those of enterovirus 71 apparently circulates among the population of Moscow.

[Efficient rabbit immunization schemes for obtaining diagnostic sera against adenoviruses]. / Ratsional'nye skhemy immunizatsii krolikov dlia polucheniia diagnosticheskikh syvorotok protiv adenovirusov.

Virus-neutralizing and hemagglutinating antibodies in higher titers can be obtained by using the schemes designed for long-term immunization of rabbits, while complement-fixing antibodies in higher titers accumulate in the blood serum after the injection of the antigen into lymph nodes.

[Rational method for producing sera with high titers of virus-neutralizing antibodies. Part III]. / Ratsional'nyi metod polucheniia syvorotok s vysokimi titrami virusnei tralizuiushchikh antitel. Soobshchenie III.

The effect of a number of nonspecific stimulators of immunogenesis (complete-Freund's adjuvant, complete adjuvant prepared from Soviet made components, arlacel with vaseline oil, aluminium hydroxide) on the immune process has been studied. The highest titers of virus-neutralizing antibodies have been obtained with the use of complete Freund's adjuvant land complete adjuvant prepared from Soviet components (X=1 : 54040 and X=1 : 40960, respectively).

[Rational method of obtaining sera with a high titre of virus-neutralizing antibodies. Report 2]. / Ratsional'nyi metod polucheniia syvorotok s vysokim titrom virusneitralizuiushchikh antitel. Soobshchenie 2.

In addition to report I (ZHMEI, 1977, No. 1) a study was made of 9 more schemes of rabbit immunization with the poliomyelitis virus, type I, for the purpose of obtaining the neutralizing sera of high titre. Vitamins A and C were used in the experiments in the capacity of the activators of the organism reaction; Freund's adjuvant of different make was tested; different reimmunization periods and different amounts of the adjuvant were administered. Titration of rabbit sera in the process of immunization and reimmunization showed immunization into the lymph nodes with the subsequent single reimmunization in one month to be the most effective and economical method of obtaining high effective sera.

[Rational method of producing sera with high titers of virus neutralizing antibodies]. / Ratsional'nyi metod polucheniia syvorotok s vysokim titrom virusneitralizuiushchikh antitel

A possibility of obtaining the diagnostic specific sera with a high titre against the poliovirus, type I, was studied experimentally. Five different immunization schemes by the same antigen were tested in parallel experiments. A method of the viral antigen (both of the concentrated and of the unconcentrated) mixed with complete Freund's adjuvant injection into the popliteal lymph nodes with the subsequent single reimmunization proved to be most effective. In this way it was possible to obtain type-specific sera with a high titre (1:20 000--1:40 000) in the course of five weeks.