Phenotypic, molecular, and functional characterization of COQ7-related primary CoQ10 deficiency: Hypomorphic variants and two distinct disease entities.

Primary coenzyme Q10 (CoQ10) deficiency is a group of inborn errors of metabolism caused by defects in CoQ10 biosynthesis. Biallelic pathogenic variants in COQ7, encoding mitochondrial 5-demethoxyubiquinone hydroxylase, have been reported in nine patients from seven families. We identified five new patients with COQ7-related primary CoQ10 deficiency, performed clinical assessment of the patients, and studied the functional effects of current and previously reported COQ7 variants and potential treatment options. The main clinical features included a neonatal-onset presentation with severe neuromuscular, cardiorespiratory and renal involvement and a late-onset disease presenting with progressive neuropathy, lower extremity weakness, abnormal gait, and variable developmental delay. Baker's yeast orthologue of COQ7, CAT5, is required for growth on oxidative carbon sources and cat5Δ strain demonstrates oxidative growth defect. Expression of wild-type CAT5 could completely rescue the defect; however, yeast CAT5 harboring equivalent human pathogenic variants could not. Interestingly, cat5Δ yeast harboring p.Arg57Gln (equivalent to human p.Arg54Gln), p.Arg112Trp (equivalent to p.Arg107Trp), p.Ile69Asn (equivalent to p.Ile66Asn) and combination of p.Lys108Met and p.Leu116Pro (equivalent to the complex allele p.[Thr103Met;Leu111Pro]) partially rescued the growth defects, indicating these variants are hypomorphic alleles. Supplementation with 2,4 dihydroxybenzoic acid (2,4-diHB) rescued the growth defect of both the leaky and severe mutants. Overexpression of COQ8 and 2,4-diHB supplementation synergistically restored oxidative growth and respiratory defect. Overall, we define two distinct disease presentations of COQ7-related disorder with emerging genotype-phenotype correlation and validate the use of the yeast model for functional studies of COQ7 variants.

Psychometric Properties of the Iowa Fluoride Study Oral Health Questionnaire in Mexican Adolescents.

BACKGROUND AND OBJECTIVE: The perception of quality of life about health status is subjective; assessment of the impact includes well-being while eating, speaking, smiling, interacting with others, and feeling good about the esthetic appearance of teeth and mouth. The objective of the present study was to cross-culturally adapt and determine the validity and reliability of the Mexican version of the Iowa Fluoride Study Oral Health for adolescents. MATERIAL AND METHODS: A cross-sectional study was carried out in a sample of 240 Mexican adolescents aged 15 to 19 years. The questionnaire was translated, back-translated, and administered through the Google Forms platform. The cultural adaptation consisted of the evaluation of the grammatical, conceptual, and linguistic equivalences. The evaluation of the utility and acceptability was carried out through an analysis of semantic equivalence. The utility of the questionnaire was also evaluated by analyzing its grammatical readability. Reliability tests, Kaiser-Meyer-Olkin (KMO), factor analysis, and Pearson's correlation were performed. RESULTS: The mean age of all participants was 16.4±1.4; 65.3% (n=158) were female. Face validity was considered adequate. The wording of the objective and instructions of the questionnaire were improved. The confidentiality assurances were highlighted. The questions were clear, understandable, and pertinent, and they showed adequate syntax. The INFLESZ index corresponds to a "fairly easy" level of readability. In the quantitative validation, the correlation of items was greater than 0.4. The KMO was 0.930 (p=0.001), and Bartlett sphericity was 2466.5 (p=0.001). Through the exploratory factorial analysis, we evaluated the emotional well-being (12 items), social welfare (five items), and oral symptoms (OS) (three items) dimensions. Internal consistency was high (Cronbach's α=0.942). CONCLUSION: The culturally translated and adapted questionnaire is valid and reliable for use in research on Mexican adolescents.

Low-Dose ACTH Stimulation Test in Obesity: A Randomized Dose Assessment.

Background: The short cosyntropin test is widely used for adrenal insufficiency screening and diagnosis. Lower cosyntropin doses may have greater sensitivity vs. the standard dose in detecting adrenal dysfunction. Obesity and overweight are increasing, impacting the clinical presentation of some diseases. Currently more than 50% of the subjects diagnosed with autoimmune adrenal insufficiency have a BMI greater than 25, and hence individuals living with overweight and obesity are more frequently requiring evaluation of the adrenal cortical function. Fixed-dose cosyntropin stimulation may not be appropriate for individuals with obesity. Objective: The primary objective was to compare cortisol response to a weight-adapted cosyntropin dose vs. a fixed low dose (1 µg) and a more physiologically fixed dose (10 µg). Methods: Twenty individuals with obesity and 20 age-matched healthy controls underwent in a randomized sequence at least one-week apart, to the The short cosyntropin test with three different doses, 0.2 µg/kg of body weight, 1 or 10 µg fixed dose stimuli. The assessment and data analysis were blinded to the individual and the investigator. Results: Cortisol response was reduced in the group with obesity with the 1 µg fixed dose stimuli at 30 minutes (median, IQR) 649.6 µg, 567.3-738.4 µg for the control group vs. 568.6 µg, 528.4-623.13 µg, p=0.04; there was a lower cortisol peak at 60' in all the three evaluated doses, with a dose-dependent trend. A weight-adapted cosyntropin dose of 0.2 µg in obesity produces a similar response to the one observed in individuals without obesity. The 1 µg ACTH test falls short on stimulating the cortisol adrenal response in individuals with obesity.

El impacto de la hipomineralización molar incisivo en la calidad de vida de los niños brasileños / O impacto da hipomineralização molar incisivo na qualidade de vida de crianças brasileiras / The impact of molar incisor hypomineralization in the quality of life in Brazilian school children

El objetivo de este estudio fue verificar la prevalencia de HMI, la severidad y el impacto en la calidad de vida de niños de 8 a 10 años. Un total de 864 niños de ambos sexos fueron evaluados en la ciudad de Vila Velha, ES. La valoración clínica del HMI estuvo de acuerdo con los criterios diagnósticos de la EAPD. Como criterio de exclusión de la investigación se consideraron niños con déficit cognitivo, neuropsicomotor y pacientes con aparatología de ortodoncia. La condición leve o severa se determinó evaluando la necesidad de tratamiento 1 y la afectación de los incisivos y molares permanentes. Para evaluar el impacto en la calidad de vida se aplicó el Cuestionario de Percepción Infantil, con 29 preguntas relacionadas con síntomas orales, limitaciones funcionales, bienestar emocional y social. Los resultados se analizaron después de las pruebas estadísticas: Chi Cuadrado, Fischer y Poisson. Se encontró una frecuencia de 183 niños (21 %) con HMI, 125 (68,3 %) con el tipo severo que necesita tratamiento. En la aplicación de CPQ, la sensibilidad y el dolor en los dientes afectados tuvieron una puntuación más alta, y cuando asociado con la presencia o ausencia del HMI, los individuos afectados tuvieron un mayor impacto en este dominio (P = 0,04). Se concluye que el HMI afecta la población estudiada en condición severa, requiriendo cuidados odontológicos preventivos y reparadores, y puede tener un impacto negativo en la calidad de vida de los estudiantes en relación a su salud bucal, en cuanto a síntomas bucales.

O objetivo deste estudo foi verificar a prevalência da HMI, a severidade e o impacto na qualidade de vida, em crianças dos 8 aos 10 anos de idade. Foi avaliado um total de 864 crianças de ambos os sexos, na cidade de Vila Velha, ES. A avaliação clínica da HMI foi de acordo com os critérios diagnósticos da EAPD. Como critério de exclusão da investigação foram consideradas crianças com déficits cognitivos, neuropsicomotores e portadoras de aparelhos ortodônticos. A condição leve ou severa foi determinada através da avaliação da necessidade de tratamento 1 e do envolvimento de molares e incisivos permanentes. Para avaliar o impacto na qualidade de vida, foi aplicado o Questionário de Percepção Infantil, composto por 29 perguntas relacionadas aos sintomas orais, limitações funcionais, bem-estar emocional e social. Os resultados foram analisados após os testes estatísticos: Chi Square, Fischer e Poisson. Foi encontrada uma frequência de 183 crianças (21 %) com HMI, com 125 (68,3 %) com o tipo severo a necessitar de tratamento. Na aplicação do CPQ, a sensibilidade e a dor nos dentes afetados apresentaram uma pontuação mais elevada, e quando este instrumento foi associado à presença ou ausência do HMI, os indivíduos afetados também tiveram um maior impacto neste domínio (P = 0,04). Concluímos que a HMI afeta a população estudada na condição do tipo severa, exigindo cuidados dentários preventivos e restauradores, e pode ter um impacto negativo na qualidade de vida dos estudantes em relação à sua saúde oral, no que diz respeito aos sintomas orais.

Purpose of this study was to verify the prevalence of MIH, the severity and the impact on the quality of life, in children from 8 to 10 years of age. Materials and methods: A total of 864 children of both genders, in the city of Vila Velha, ES, were evaluated. The clinical assessment followed the diagnostic criteria of the EAPD. As exclusion criterion of the research were children with cognitive deficits, neuropsychomotor and carriers orthodontic appliances. The mild or severe conditions were determined by assessing the need for treatment (WHO) and involvement of permanent molars and incisors. To evaluate the impact on quality of life, the Child Perception Questionnaire was applied, composed of 29 questions elaborated to observe the child's perception regarding oral symptoms, functional limitations, well-being Emotional and social. For statistical analysis, Chi, Square, Fischer and Poisson tests were used. Results: A frequency of 183 children (21%) with MIH was found, with 125 (68.3%) with the severe type requiring treatment. In the application of CPQ, the sensitivity and pain in affected teeth presented a higher score, and when this instrument was associated with the presence or absence of MHI, the affected individuals also had a greater impact in this domain (P = 0.04). Conclusions: The MIH affects the studied population in severe condition, requiring preventive and restorative dental care, and can negatively impact the quality of life of the students in relation to their oral health, as far as oral symptoms are concerned

Narrativas de jóvenes con intentos suicidas: una mirada al cuerpo y la micropolítica / Narratives of young people with suicide attempts: a look at the body and micropolitics

Este artículo es el resultado de una investigación que da cuenta de la relación entre el intento de suicidio y la micropolítica en jóvenes de una institución educativa del municipio de Chinchiná, Caldas, Colombia. El referente teórico se basa en las discusiones en torno a los conceptos de micropolítica y cuerpo. La fundamentación epistemológica y metodológica fue fenomenológica hermenéutica. Todo esto dio la posibilidad de que los jóvenes en sus narrativas autobiográficas relataran sus vidas y encontraran nuevas posibilidades de existencia. Se encontraron categorías como: el encuentro con el mundo, un mundo sin cuidado, la violencia sexual, el cuerpo como espacio de comunicación, la incertidumbre, entre otras. Se concluye la importancia de comprender los procesos de singularización de los jóvenes para interpretar los sentidos que le dan al cuerpo y su relación con la micropolítica; asimismo, cómo los jóvenes manifiestan su rechazo a la producción de subjetividad capitalista a través del intento de suicidio

This article is the result of an investigation that gives an account of the relationship between suicide attempts and micropolitics in young people of an educational institution in the municipality of Chinchiná, Caldas, Colombia. The theoretical reference is based on discussions around the concepts of micropolitics and the body. The epistemological and methodological foundation was hermeneutic phenomenological. All this gave the possibility for young people in their autobiographical narratives to relate their lives and find new possibilities of existence Categories such as the encounter with the world, a world without a care, sexual violence, the body as a space of communication, uncertainty, among others, were found. It concludes the importance of understanding the processes of singularization of young people to interpret the senses they give to the body and its relationship with micropolitics. Likewise how young people manifest their rejection of the production of capitalist subjectivity through the attempt of suicide

Physiological testosterone replacement effects on male aged rats with orchiectomy-induced osteoporosis in advanced stage: a tomographic and biomechanical pilot study.

Aim: This study aimed to evaluate the effect of physiological testosterone replacement on male aged rats with orchiectomy-induced osteoporosis in advanced stage.Methods: Thirty male rats (Rattus norvegicus albinus, Holtzman lineage) were randomly distributed into 3 groups (n = 10): 1-sham, 2-orchiectomy (OCX), 3-OCX + testosterone replacement (OCX + T). On day 0, a sham or orchiectomy surgery was performed according to the groups. Thirty and sixty days after surgeries, the animals from OCX + T group received testosterone intramuscularly, and the rats in all groups were euthanized on day 77. The femurs were removed for micro-CT scanning and biomechanical test.Results: Orchiectomy resulted in a marked trabecular bone damage (p < 0.05), which was not reversed with testosterone treatment (OCX + T group). The femoral strength was lower in orchiectomized animals (p < 0.05), while the bone strength in OCX + T group was similar to that observed in the sham animals (p > 0.05) and correlated to this parameter the deformation of rupture was smaller in OCX + T group.Conclusion: In conclusion, testosterone depletion induced by orchiectomy established an osteoporotic environment, mainly affecting the trabecular bone. Moreover, even though testosterone treatment did not enhance these variables, the hormonal replacement improved the femoral fracture strength and promoted beneficial effects on the biomechanical parameters compromised by castration in femoral bone.

Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages.

Natural products have emerged as a rich source of bioactive compounds for adjunctive treatments of many infectious and inflammatory conditions, including periodontitis. Among the monoterpenes with significant biological properties, there is the perillyl alcohol (POH), which can be found in several essential oils and has shown immunomodulatory properties in recent studies, which may be interesting in the treatment of non-neoplastic inflammatory disorders. Objective To determine the antibacterial and immune modulatory activities of the POH. Methodology The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the POH for two significant Gram-negative periodontal pathogens were determined by macrodilution and subculture, respectively. Cell proliferation and cytotoxicity in RAW 264.7 macrophages were determined by Trypan Blue and mitochondrial enzymatic activity assay. The modulation of reactive oxygen species (ROS) was analyzed by flow cytometry and expression of TNF and arginase-1 by real-time PCR. Results The POH was effective against P. gingivalis (ATCC 33277) and F. nucleatum (ATCC 25586) with MIC= MBC=1600 µM. No cytotoxicity up to 100 µM was observed on macrophages. The cell proliferation was inhibited from 48 hours at 100 µM (p<0.05) and 250 µM (p<0.01). The POH increased ROS production at both 10 µM and 100 µM (p<0.05) in unstimulated cells. The PMA-induced ROS production was not affected by POH, whereas 100 µM significantly reduced lipopolysaccharide-induced (LPS-induced) ROS. The expression of TNF was not affected by POH in unstimulated cells or in cells polarized to M1 phenotype, whereas both concentrations of POH reduced (p<0.05) the expression of arginase-1 in M2-polarized macrophages. Conclusion The POH has antibacterial activity against periodontal pathogens and reduced proliferation of murine macrophages without significant cytotoxicity at concentrations up to 100 µM. In addition, the POH reduced the LPS-induced ROS and the expression of arginase-1 in M2-polarized macrophages.

The challenge of diagnosing atypical odontalgia. Case report / O desafio para o diagnóstico da odontalgia atípica. Relato de caso

ABSTRACT BACKGROUND AND OBJECTIVES: Atypical odontalgia is characterized by continuous pain that persists for more than three months in one or more teeth or in the socket after extraction, without apparent dental and neurological causes, with transient pain relief and worsening within a few days or even weeks in patients undergoing extensive dental treatment. These patients are at risk of going through unnecessary dental/surgical procedures, which would worsen their pain. This article aims to report a difficult case of atypical odontalgia diagnosis of a patient that underwent extensive dental treatment accompanied by severe pain. CASE REPORT: A 57-year-old female patient with severe and excruciating pain in the right maxillary region of no identified source. After an endodontic retreatment on teeth 14 and 16, the pain worsened suggesting traumatic pericementitis or reaction to the intracanal drug used. Since the pain did not improve, a pulpectomy on 13 was performed. However, the pain increased significantly, and after an evaluation by volumetric computed tomography, a paraendodontic surgery was performed, but the pain irradiated to the ocular fundus and the maxillary region. The absence of neurological, ophthalmological, and otorhinolaryngological alterations led to the diagnosis of atypical odontalgia. CONCLUSION: The diagnosis of atypical odontalgia is difficult, requiring a multidisciplinary approach listening to the patient's complaint, and, in case of doubt, avoid any procedures not to worsen pain and turn it chronic.

RESUMO JUSTIFICATIVA E OBJETIVOS: A odontalgia atípica caracteriza-se por dor contínua que persiste por mais de três meses em um ou mais dentes ou no alvéolo após extração, sem causas dentárias e neurológicas aparentes, com alívio transitório, e piora da dor dentro de poucos dias ou até semanas, em pacientes com amplo tratamento odontológico. A dificuldade para o diagnóstico pode levar a procedimentos odontológicos desnecessários e mutiladores, com piora e/ou cronificação da dor. Este artigo tem como objetivo relatar um caso de difícil diagnóstico de odontalgia atípica em paciente submetida a extenso tratamento odontológico. RELATO DO CASO: Paciente do sexo feminino, 57 anos, apresentava queixa de dor lancinante em região maxilar direita cuja origem não identificava. Após retratamento endodôntico dos dentes 14 e 16, houve piora da dor sugerindo pericementite traumática ou reação a fármaco intracanal. Como a dor não melhorou foi realizada uma pulpectomia no dente 13. Entretanto, a dor aumentou e após tomografia computadorizada volumétrica foi realizada cirurgia parendodôntica, porém a dor irradiou para fundo do olho direito e região maxilar. A ausência de alterações neurológicas, oftalmológicas e otorrinolaringológicas permitiram diagnosticar a odontalgia atípica. CONCLUSÃO: O diagnóstico é difícil, sendo recomendada a abordagem multidisciplinar, valorizar a queixa do paciente e, em casos de dúvida, evitar quaisquer procedimentos para não piorar e cronificar a dor.

Perillyl alcohol has antibacterial effects and reduces ROS production in macrophages

Abstract Natural products have emerged as a rich source of bioactive compounds for adjunctive treatments of many infectious and inflammatory conditions, including periodontitis. Among the monoterpenes with significant biological properties, there is the perillyl alcohol (POH), which can be found in several essential oils and has shown immunomodulatory properties in recent studies, which may be interesting in the treatment of non-neoplastic inflammatory disorders. Objective To determine the antibacterial and immune modulatory activities of the POH. Methodology The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the POH for two significant Gram-negative periodontal pathogens were determined by macrodilution and subculture, respectively. Cell proliferation and cytotoxicity in RAW 264.7 macrophages were determined by Trypan Blue and mitochondrial enzymatic activity assay. The modulation of reactive oxygen species (ROS) was analyzed by flow cytometry and expression of TNF and arginase-1 by real-time PCR. Results The POH was effective against P. gingivalis (ATCC 33277) and F. nucleatum (ATCC 25586) with MIC= MBC=1600 μM. No cytotoxicity up to 100 µM was observed on macrophages. The cell proliferation was inhibited from 48 hours at 100 μM (p<0.05) and 250 μM (p<0.01). The POH increased ROS production at both 10 μM and 100 μM (p<0.05) in unstimulated cells. The PMA-induced ROS production was not affected by POH, whereas 100 μM significantly reduced lipopolysaccharide-induced (LPS-induced) ROS. The expression of TNF was not affected by POH in unstimulated cells or in cells polarized to M1 phenotype, whereas both concentrations of POH reduced (p<0.05) the expression of arginase-1 in M2-polarized macrophages. Conclusion The POH has antibacterial activity against periodontal pathogens and reduced proliferation of murine macrophages without significant cytotoxicity at concentrations up to 100 μM. In addition, the POH reduced the LPS-induced ROS and the expression of arginase-1 in M2-polarized macrophages.

Atypical odontalgia: pathophysiology, diagnosis and management / Odontalgia atípica: fisiopatologia, diagnóstico e tratamento

ABSTRACT BACKGROUND AND OBJECTIVES: Atypical odontalgia, a subtype of persistent idiopathic facial pain, is characterized by continuous pain in one tooth or more, or inside the alveolus after exodontia, with no apparent clinical causes. These patients run the risk of going through unnecessary dental/surgical procedures which would worsen their pain. Since the pathophysiology, diagnosis, and management of atypical odontalgia are not clear, this article aims to present an integrative literature review about these aspects. CONTENTS: A review of articles related to the topic was conducted on the Pubmed database using the keywords "atypical odontalgia" OR "phantom tooth pain" OR "idiopathic tooth pain" OR "odontalgia" OR "odontalgias" OR "atypical toothache". Applying the inclusion criteria (publications in the last ten years, in English, as clinical trials, multicenter studies, case reports, reviews, integrative and systematic reviews, 114 articles were found, and 39 were selected after the application of the exclusion criteria (articles with no relation to the topic). CONCLUSION: Although studies suggest the involvement of strong neuropathic mechanism, the psychological/psychiatric aspects might be considered not as a primary cause, but as an aggravator of the patient´s pain. Knowledge of other pathologies is recommended in order to determine the differential diagnosis. Also, complementary image tests, qualitative somatosensorial test, and reference to an orofacial pain specialist should be considered. In case of uncertain diagnosis, it is recommended to avoid any dental procedures because the pain can get worse.

RESUMO JUSTIFICATIVA E OBJETIVOS: A odontalgia atípica, um subtipo da dor facial idiopática persistente, se caracteriza por dor contínua em um ou mais dentes, ou no alvéolo, após exodontia sem qualquer causa aparente e é um desafio para o dentista. O desconhecimento por parte do profissional pode levar a procedimentos odontológicos desnecessários e mutiladores, piorando e/ou cronificando a dor do paciente. Diante desse panorama, o objetivo deste estudo foi apresentar informações referentes à fisiopatologia, diagnóstico e tratamento da odontalgia atípica através de uma revisão integrativa da literatura. CONTEÚDO: A busca na base de dados Pubmed foi realizada com os termos: "atypical odontalgia" OR "phantom tooth pain" OR "idiopathic tooth pain" OR "odontalgia" OR "odontalgias" OR "atypical toothache". Aplicando-se critérios de inclusão (publicações nos últimos 10 anos, de língua inglesa, tipo ensaio clínico, estudo multicêntrico, relato de caso, revisão, revisão integrativa científica e sistemática) foram encontrados 114 artigos, dos quais 39 foram selecionados após aplicação do critério de exclusão (trabalhos sem relação com o tema). CONCLUSÃO: Embora os estudos apontem forte envolvimento de mecanismos neuropáticos, aspectos psicogênicos/psiquiátricos devem ser levados em consideração como agravante do estado de dor do paciente. Sugere-se conhecimento sobre as outras doenças existentes para se realizar um diagnóstico diferencial, exames complementares de imagem, realização do teste somatossensorial qualitativo, encaminhamento a um especialista em dor orofacial e neurologista, e em casos de dúvida, não realizar nenhum procedimento a fim de não piorar a sua dor.

Importance of Dental Clinic Recalls for Caries Prevention in Children: Practice-Based Research.

Objective: to investigate the association between caries incidence in children and the number of clinical preventive visits and follow-up period (intervals). Study Design: a 30-year cohort composed of the dental records of 600 children who were 12-23 months old at their first dental appointment and who were followed up to 12 years of age (200 followed since 1981, Group 1; 200 followed since 1991, Group 2; and 200 followed since 2001, Group 3) was analytically and quantitatively evaluated. Random sample calculation was performed with 95% confidence, a maximum error of 2.95% and a ratio of 50%. Results: association was found between caries incidence and the number of dental visits and consultation intervals. Children who attended a smaller number of preventive visits had a higher incidence of the disease. Children who had an interval between returns greater than 12 months had an 18.7-times greater caries risk compared to children who had intervals no longer than 8 months between return visits. Conclusions: preventive consultations with an average interval of up to 8 months seemed to increase family adherence to preventive procedures and acted as a protective factor against caries incidence in children.

Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding blaNDM-like genes as determined by WGS.

OBJECTIVES: NDM carbapenemases have spread worldwide. However, little information exists about the impact of NDM-producing Enterobacteriaceae in Spain. By WGS, we sought to elucidate the population structure of NDM-like-producing Klebsiella pneumoniae and Escherichia coli in Spain and to determine the plasmids harbouring blaNDM-like genes. METHODS: High-resolution SNP typing, core-genome MLST and plasmid reconstruction (PlasmidID) were performed on 59 NDM-like-producing K. pneumoniae and 8 NDM-like-producing E. coli isolated over an 8 year period in Spain. RESULTS: Five major epidemic clones of NDM-producing K. pneumoniae caused five important nationwide outbreaks: ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1; in contrast, the spread of NDM-producing E. coli was polyclonal. Three blaNDM types were identified: blaNDM-1, 61.2%; blaNDM-7, 32.8%; and blaNDM-5, 6%. Five K. pneumoniae isolates co-produced other carbapenemases (three blaOXA-48 and two blaVIM-1). The average number of acquired resistance genes was higher in K. pneumoniae than in E. coli. The plasmids encoding blaNDM-like genes belonged to IncFII, IncFIB, IncX3, IncR, IncN and IncC types, of which IncF, IncR and IncC were associated with MDR. The genetic surroundings of blaNDM-like genes showed a highly variable region upstream of ISAba125. CONCLUSIONS: In recent years NDM-producing K. pneumoniae and E. coli have emerged in Spain; the spread of a few high-risk K. pneumoniae clones such as ST437/NDM-7, ST437/NDM-1, ST147/NDM-1, ST11/NDM-1 and ST101/NDM-1 have caused several interregional outbreaks. In contrast, the spread of NDM-producing E. coli has been polyclonal. Plasmid types IncFII, IncFIB, IncX3, IncR, IncN and IncC carried blaNDM, and the same IncX3 plasmid was detected in K. pneumoniae and E. coli.

Oral characteristics and medical considerations in the dental treatment of individuals with Williams syndrome.

AIM: The aim of this study was to evaluate oral characteristics and comorbidities that may affect dental treatment of individuals with Williams syndrome (WS). METHODS AND RESULTS: Fifty-two subjects diagnosed with WS were included in this observational study. Demographic data and medical history were compiled. Facial aspects, tooth abnormalities and oral characteristics were obtained through clinical and radiographic evaluation by a researcher/dentist. Among 52 subjects, 25 were also evaluated for temporomandibular and occlusal disorders, caries and periodontal disease. From the 52 subjects, 23 (44.2%) were female and the average age was of 20 years old (range from 4 to 35 years old). Cognitive impairment and congenital heart disease were the most common medical disorders found in all 52 (100%) and in 41 (78.8%) subjects, respectively. Among the 52 subjects, 51 (98%) presented at least one dental developmental abnormality, with generalized diastemas (72.5%) and hypodontia (50.9%) being the most frequent ones. Angle class III malocclusion was observed in 52% (13/25) of the subjects. CONCLUSIONS: The dentist should be aware of the medical conditions of individuals with WS and thus offer an adequate dental treatment. The high prevalence of tooth abnormalities and occlusal disorders requires an early dental treatment planning.

Violencias cotidianas: perspectivas situadas desde las experiencias de niñas y mujeres en el municipio de Medellín, Colombia / Violências cotidianas: perspectivas situadas nas experiências de meninas e mulheres no município de Medellín, Colômbia / Everyday violences: perspectives on the experiences of girls and women in the municipality of Medellín, Colombia

Resumen: Este artículo de resultados emerge como parte del análisis de algunos aspectos del trabajo de campo que se generó en el año 2015, en el marco de la investigación "Procesos de construcción social de la niñez en contextos de conflicto armado en el Eje Cafetero, Antioquia y Área Metropolitana de Bogotá: la paz, la reconciliación y la democracia desde la perspectiva de narrativas generativas de niños y niñas". El artículo evidencia, a partir del análisis de las narrativas de niños, niñas y mujeres -participantes de la investigación en una de las instituciones educativas que hicieron parte del proceso investigativo-, las vulneraciones a las que se ven expuestas las niñas y mujeres desde los estereotipos de género y las violencias cotidianas. Como hallazgo central se encontró que, aunque el conflicto armado colombiano generó violencias contra las mujeres y las niñas, ellas han vivido y siguen viviendo cotidianamente violencias, que afectan de múltiples maneras sus vidas y sus cuerpos, las cuales se encuentran fuertemente naturalizadas y coexisten con la violencia armada o la justifican.

Resumo: Os resultados deste artigo emergem como parte da análise de alguns aspectos do trabalho de campo realizado em 2015, no contexto da pesquisa "Procesos de construcción social de la niñez en contextos de conflicto armado en el Eje Cafetero, Antioquia y Área Metropolitana de Bogotá: la paz, la reconciliación y la democracia desde la perspectiva de narrativas generativas de niños y niñas". O artigo evidencia, a partir da análise das narrativas de crianças e mulheres que participam da pesquisa em uma das instituições de ensino que fizeram parte do processo de investigação, as violações a que estão expostas por causa de estereótipos de gênero e violência cotidiana. Verificou-se que, embora o conflito armado colombiano levou à violência contra mulheres e meninas, elas viveram e ainda vivem a violência diária que afetam de várias maneiras suas vidas e seus corpos, que são fortemente naturalizadas e coexistem com a violência armada ou a justifica.

Abstract: This article's results emerge as part of the analysis of some aspects of a 2015 field work within the framework of the research "Procesos de construcción social de la niñez en contextos de conflicto armado en el Eje Cafetero, Antioquia y Área Metropolitana de Bogotá: la paz, la reconciliación y la democracia desde la perspectiva de narrativas generativas de niños y niñas". The article evidences, from the analysis of the narratives of children and women -participants of the research in one of the educational institutions that were part of the investigative process-, the violations to which girls and women are exposed because of gender stereotypes and everyday violence. Although the Colombian armed conflict generated violence against women and girls, they have lived and continue to experience daily violence, which affects their lives and bodies in many ways, which are strongly naturalized and coexist with armed violence or justify it.

Craneosinostosis sindrómica: Características craneodentofaciales, tratamiento ortodón-tico-quirúrgico y factores asociados a tipos de síndrome / Craniossinostose sindrômica: Características craniodentofaciais, tratamento ortodônti-co-cirúrgico e fatores associados aos tipos de síndrome / Syndromic Craniosynostosis: Craniodentofacial features, orthodontic-surgical treatment and factors associated to types of syndrome

Objetivos: Describir las características craneodentofaciales, tratamientos ortodónticos-quirúrgicos y establecer una aso-ciación entre los tipos de síndrome presentes en pacientes con craneosinostosis sindrómica (CS). Material y métodos: Estudio retrospectivo de registros médicos y de ortodoncia de pacientes con CS. Los datos se recogieron en una forma específica y se sometieron a un análisis estadístico descriptivo para observar la distribución de frecuencias y se utilizó la prueba de Chi cuadrado con un nivel de significación del 5% para asociar el síndrome y los tipos de variables. Resultados: El síndrome de Crouzon fue el tipo predominante (59.2%) y la braquicefalia fue el tipo más frecuente de craneosinostosis (63.6%). Hubo una asociación significativa entre las variables braquicefalia (p = 0,014), presencia de paladar hendido (p = 0,043), mordida cruzada posterior (p = 0,013), distracción osteogénica realizada por elásticos intermaxilares (p = 0,030), barra de Erich (p = 0.007) y la extracción (p = 0.041) y los síndromes estudiados. Conclusión: Los pacientes con CS a menudo tienen cambios craneodentofaciais y algunas variables tienen asociaciones significativas en relación con los tipos de síndromes.

Objectives: Describe the craniodentofacial characteristics, orthodontic-surgical treatments and establish an association between the syndrome types present in patients with syndromic craniosynostosis (SC). Material and methods: Retros-pective study of medical and orthodontic records of patients with SC. Data was collected on a specific form and subjected to descriptive statistical analysis to observe the distribution of frequencies and chi-square test with level of significance of 5% was used to associate syndrome and the types of variables. Results: Crouzon syndrome was the predominant type (59.2%) and brachycephalia was the most prevalent type of craniosynostosis (63.6%). There was a significant association between the variables brachycephaly (p=0.014), presence of cleft palate (p=0.043), posterior cross bite (p=0.013), distrac-tion osteogenesis performed by intermaxillary elastics (p= 0.030), Erich bar (p= 0.007) and extraction (p=0.041) and the syndromes studied. Conclusion: Patients with SC often have craniodentofaciais changes and some variables had signifi-cant associations in relation to the types of syndromes.

Descrever as características craniodentofaciais, tratamentos ortodôntico-cirúrgicos e estabelecer uma associação entre os tipos de síndrome presentes em pacientes com craniossinostose sindrômica (CS). Material e métodos: Estudo retrospectivo de prontuários médicos e ortodônticos de pacientes com CS. Os dados foram coletados em formulário específico e submetidos à análise estatística descritiva para observar a distribuição das frequências e o teste qui-quadrado com nível de significância de 5% foi utilizado para associar a síndrome e os tipos de variáveis. Resultados: A síndrome de Crouzon foi o tipo predominante (59,2%) e a braquicefalia foi o tipo mais prevalente de craniossinostose (63,6%). Houve associação significativa entre as variáveis braquicefalia (p = 0,014), presença de fissura de palato (p = 0,043), mordida cruzada posterior (p = 0,013), distração osteogênica realizada pelos elásticos intermaxilares (p = 0,030), barra de Erich (p= 0,007) e extração (p = 0,041) e as síndromes estudadas. Conclusão: Pacientes com CS frequentemente apresentam alterações craniodentofaciais e algumas variáveis apresentaram associações significativas em relação aos tipos de síndromes.

Long-term testosterone depletion attenuates inflammatory bone resorption in the ligature-induced periodontal disease model.

BACKGROUND: Testosterone is known to affect bone in physiological and pathological conditions. The purpose of this study is to evaluate the role of testosterone in experimental periodontal disease in rats. METHODS: In this study we used a ligature model of periodontal disease in rats submitted to orchiectomy (OCX, testosterone depletion) with and without testosterone replacement therapy (TR). Control animals were sham-operated and retained physiological testosterone levels. Sixty-two days after orchiectomy and sham operations, ligatures were placed around the lower first molars for 2 weeks to induce experimental periodontal disease. Negative control animals received no ligatures. The outcomes assessed in the periodontal tissues were: inflammatory cytokine expression by enzyme-linked immunosorbent assay (ELISA), stereometric analysis of the inflammatory process and quantitation of inflammatory bone resorption by microcomputed tomography (µ-CT). RESULTS: The OCX+TR group showed the greatest increase in fibroblastic cells and blood vessels with reduced inflammatory cell numbers in the gingival tissue with induction of periodontal disease. There were no significant differences between OCX and Sham-operated groups in all the stereometric parameters assessed. Ligature placement induced inflammatory bone resorption, which was significantly attenuated in OCX animals. Experimental periodontitis induced a significant increase in interleukin (IL)-1ß, but the lowest levels were observed in the periodontitis/OCX group. IL-6 levels were not affected by OCX, but were significantly reduced in OCX+TR animals. CONCLUSION: The findings of the present study suggest that testosterone depletion attenuates inflammatory bone resorption in ligature-induced periodontitis, which may be partly mediated via decreased production of IL-1ß.

Relationship between stress and sleep bruxism in children and their mothers: A case control study.

INTRODUCTION: Sleep bruxism (SB) is a frequent condition in children. Its pathophysiology involves certain neurotransmitters (serotonin, dopamine, noradrenalin, histamine), but the environment seems to influence its occurrence. OBJECTIVE: The objective of this study was to investigate the prevalence of SB in children and their biological mothers, relating it to stress through a case control study. METHODS AND MATERIAL: The group of cases (SG) was composed of 48 subjects, including 24 children with SB and their mothers, and the control group (CG), formed by 36 children without SB and their mothers. The chi-Square test was used and the probability was computed with 95% confidence intervals (p<0.05). Results: The prevalence of probable awareness of SB in children was 22.6%, while it was 30.8% among caregivers. There were no significant associations between the socio-demographic variables and SB. There was an increase in the occurrence of SB in children (p=0.02) when their caregiver also had this condition. CONCLUSIONS: Psychological stress did not establish a significant relationship with SB in either the child or their mothers.

Evaluation of oral manifestations of patients with mucopolysaccharidosis IV and VI: clinical and imaging study.

OBJECTIVE: The aim of this study is to assess oral manifestations in patients with mucopolysaccharidosis IV (MPS IVA) and mucopolysaccharidosis VI (MPS VI). MATERIALS AND METHODS: Seventeen patients were assessed, nine with MPS IVA and eight with MPS VI, treated at the Medical Genetics Outpatient Clinic of Hospital Universitário Alcides Carneiro (HUAC) in Campina Grande, Paraíba State, Brazil. Assessments included clinical and intraoral examinations, analysis of occlusal function, and panoramic X-rays. RESULTS: Among all, 64.7% of the patients had unerupted teeth and 52.9% of them had enamel hypoplasia. Regarding the temporomandibular joint, 88.2% of the patients showed flattening of the mandibular condyle, 52.9% showed condylar hypoplasia, and all of them showed decreased mobility. Enamel hypoplasia was observed only in patients with MPS IVA (p < 0.05). On the other hand, only MPS VI patients presented with anterior open bite, restricted mouth opening (p < 0.05), and a higher rate of unerupted teeth, hyperplastic tooth follicle, and condylar defects (p < 0.05). CONCLUSIONS: Enamel hypoplasia was observed only in patients with MPS IVA, whereas anterior open bite was observed only in patients with MPS VI. Abnormal findings in the maxillomandibular complex were more frequent in patients with MPS VI. CLINICAL RELEVANCE: The relevant frequency of MPS VI and IVA in the sample allows us to compare the changes occurring in both groups of patients, therefore enabling us to further comprehend the oral manifestations in specific types of MPS.