RESUMO
This study investigated changes in hematological and antioxidant parameters of carp exposed to two different doses of synthetic pyrethroid permethrin (control, vehicle, 10 ppm, and 20 ppm) for two different periods (4 days and 21 days). Hematological analyses were then performed on a veterinary Ms4 (Melet Schloesing, France) blood counter using commercially available kits (Cat. No. WD1153). Buege and Aust for MDA, Luck for CAT, McCord and Frivovich for SOD, Lawrence and Burk methods for GSH-Px were used to determine antioxidant parameters. Decreases in RBC count, Hb amount, Hct value, granulocyte ratios, and increases in total WBC and lymphocyte ratios were statistically significant in both dose groups treated with permethrin compared to the control group (p < 0.05). However, there was no statistically significant difference in monocyte ratios (p > 0.05). Overall, permethrin exposure caused an increase in MDA levels in the liver and gill tissues of carp in both dose and duration groups compared to the control group. Also, no statistically significant difference between the two dose groups in the liver tissue was observed in terms of duration (p > 0.05). Nonetheless, the increase in MDA levels in PERM10 and PERM20 dose groups in the gill tissues over 21 days was statistically significant (p < 0.05). Furthermore, permethrin exposure increased CAT, SOD, and GSH-Px enzyme activities in the gill tissue, while impacting in the opposite direction the liver tissue. Finally, regarding MDA, CAT, SOD, and GSH-Px levels, the control, and control acetone dose groups of all experimental groups were observed to be similar (p > 0.05). As a result, permethrin produced a toxic effect on Cyprinus carpio, triggering changes in blood parameters and inducing the antioxidant enzyme system.
Assuntos
Carpas , Piretrinas , Animais , Antioxidantes/metabolismo , Piretrinas/toxicidade , Permetrina/toxicidade , Carpas/metabolismo , Superóxido Dismutase/metabolismo , Estresse OxidativoRESUMO
Propolis, a resinous material produced by worker bees from the leaf buds and exudates of plants, is reported to possess various therapeutic properties. The aim of this study was to investigate the effects of propolis on biochemical parameters and histopathologic findings in carp Cyprinus carpio L. exposed to arsenic. A sublethal concentration of arsenic (0.01 mg l-1) and/or 10 mg l-1 propolis were administered to fish for 1 wk. Catalase (CAT) activities and malondialdehyde (MDA) levels were determined in liver, gill and muscle tissues in control, arsenic only, propolis only and arsenic+propolis treatment groups. Results showed that CAT activity decreased in the arsenic group compared to the control and propolis groups. CAT activity in the arsenic+propolis group was significantly higher compared to the arsenic group. MDA levels in fish exposed to 0.01 mg l-1 arsenic significantly increased compared to the control group. However, MDA levels in the arsenic+propolis group were significantly lower compared to the arsenic group. Histopathological changes in the liver, gill and muscle tissues of carp were examined by light microscopy: various changes were observed in all tissues of fish in the arsenic group. Propolis showed important antioxidant effects against arsenic toxicity in all fish tissues.
Assuntos
Antioxidantes/uso terapêutico , Intoxicação por Arsênico/veterinária , Carpas , Doenças dos Peixes/induzido quimicamente , Própole/uso terapêutico , Animais , Intoxicação por Arsênico/tratamento farmacológico , Brânquias/efeitos dos fármacos , Fígado/efeitos dos fármacos , Músculo Esquelético/efeitos dos fármacos , Estresse Oxidativo/efeitos dos fármacosRESUMO
This study showed the effects of propolis on biochemical and hematological parameters in chronic nitric oxide synthase inhibited rats by Nω-Nitro-L-arginine methyl ester (L-NAME). Rats are given L-NAME for 15 days and the propolis for the last 5 days with L-NAME together. The levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and gamma glutamyltransferase in the L-NAME group compared to control group have increased (P<0.05). The levels of these parameters in L-NAME+propolis group compared to the L-NAME group have decreased (P<0.05). L-NAME caused increase (P<0.05) in levels of glucose, albumin, globulin, creatinine, urea, triglyceride and cholesterol. Erythrocyte number, total leukocyte, hemoglobin, hematocrit, neutrophil and monocyte decreased (P<0.05), platelets and lymphocyte increased (P<0.05) in L-NAME+propolis group compared to the L-NAME group. The study concluded that homeostasis is modulated in L-NAME administrated rats by adding propolis which causes increasing generation of vascular nitric oxide.
Assuntos
Inibidores Enzimáticos/farmacologia , NG-Nitroarginina Metil Éster/farmacologia , Óxido Nítrico Sintase/antagonistas & inibidores , Óxido Nítrico/metabolismo , Própole/farmacologia , Animais , Biomarcadores/sangue , Células Sanguíneas/efeitos dos fármacos , Células Sanguíneas/metabolismo , Análise Química do Sangue , Contagem de Eritrócitos , Contagem de Leucócitos , Masculino , Óxido Nítrico Sintase/metabolismo , Ratos , Ratos Wistar , Fatores de TempoRESUMO
The main purpose of this study is to discuss the effect of Cd+2, Cr+3 and Se metals on biochemical parameters in liver tissue of Oncorhynchus mykiss. The rainbow trout were exposed to heavy metal stress (Cd+2, Cr+3) at 2 ppm dosage. The present study was undertaken to determine the protective effect of selenium treatment at the same dosage (2 ppm) on some biochemical parameters. The activity of catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and the changes in levels of malondialdehyde (MDA) from biochemical parameters were determined in liver tissue of the fish groups exposed to heavy metals, especially for the selenium-applied groups. Results of this study showed that the activities of CAT, GSH-Px and SOD in the tissues of fish exposed to the stress of Cd+2 and Cr+3 were significantly lower than the control groups (P < 0.05). Meanwhile, the closer values to the control groups were obtained in selenium-added groups (Cr+3 + Se+4, Cd+2 + Se+4). For the level of MDA, the last production of lipid peroxidation showed increases (P < 0.05) in the groups exposed to the metal stress, whereas significant decreases were obtained in selenium-applied groups. The result of the statistical evaluation showed that the negative effects occurring in the biochemical parameters of the applied groups exposed to the toxicity of heavy metal were significantly eliminated (P < 0.05) as a result of selenium treatment.
Assuntos
Antioxidantes/farmacologia , Cádmio/toxicidade , Cromo/toxicidade , Fígado/efeitos dos fármacos , Oncorhynchus mykiss/fisiologia , Selênio/farmacologia , Animais , Antioxidantes/administração & dosagem , Selênio/administração & dosagemRESUMO
This study was carried out to understand the preventive effect of selenium (Se4+) on heavy metal stress induced by lead and copper in rainbow trout (Oncorhynchus mykiss). Variation in glutathione peroxidase (Se-GSH-Px) and superoxide dismutase (SOD) activity, and in malondialdehyde (MDA) levels in liver, spleen, heart, and brain tissues of rainbow trout after 72 h of exposure to Pb2+ and Cu2+ were investigated in the presence and absence of Se4+. In the presence of Se4+, Se-GSH-Px activity and SOD activity were found to be higher and MDA levels were lower compared with in its absence. Hematological parameters were also determined and it has been observed that total leukocyte count (WBC), mean cell volume (MCV), and mean cell hemoglobin (MCH) were increased and erythrocyte number (RBC), hemoglobin (Hb), and hematocrit value (Hct; P < 0.05) were decreased in fish exposed to heavy metals in the absence of selenium. Selenium presence recovered hematological parameters to normal levels. In the light of our findings, it could be stated that Pb2+ and Cu2+ lead to dramatic changes in biochemical and hematological parameters and selenium caused these parameters to converge to control levels when it was administered concurrently with these heavy metals.
Assuntos
Cobre/toxicidade , Chumbo/toxicidade , Oncorhynchus mykiss/fisiologia , Selenito de Sódio/farmacologia , Poluentes Químicos da Água/toxicidade , Animais , Tamanho Celular , Exposição Ambiental , Contagem de Eritrócitos , Eritrócitos/química , Eritrócitos/efeitos dos fármacos , Glutationa Peroxidase/metabolismo , Hematócrito , Hemoglobinas/análise , Contagem de Leucócitos , Leucócitos/química , Leucócitos/efeitos dos fármacos , Malondialdeído/metabolismo , Superóxido Dismutase/metabolismoRESUMO
DMBA (7,12-dimethylbenz[a]anthracene) is a polycyclic aromatic hydrocarbon (PAH) known to cause tumors in rats. Selenium is an essential element with physiological non-enzymatic antioxidant properties. Because of the health problems induced by many environmental pollutants, many efforts have been undertaken in evaluating the relative antioxidant potential of selenium and synthetic organoselenium compounds. In this study, adult female Wistar rats were treated with DMBA and the novel organoselenium compounds (1-isopropyl-3-methylbenzimidazole-2-selenone [SeI] and 1,3-di-p-methoxybenzylpyrimidine-2-selenone [SeII]) in the determined doses. The protective effects of novel synthetic organoselenium compounds (SeI and SeII) against DMBA-induced changes in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) activities and total glutathione (GSH) and malone-dialdehyde (MDA) levels of rat heart and brain were investigated. It was determined that SeI and SeII fully or partially restored enzyme activity. It was also found that lipid peroxidation was also decreased in SeI and SeII treated groups. Consequently, it was determined that novel synthetic organoselenium compounds (SeI and SeII) provided protection of antioxidant activity, and protection against lipid peroxidation measured as MDA in SeI and SeII treated groups was provided by novel synthesized organoselenium compounds. The ability of the organoselenium compounds to prevent oxidative damage induced by DMBA in rats was rationalized.
Assuntos
Antioxidantes/farmacologia , Compostos Organosselênicos/farmacologia , Animais , Antioxidantes/síntese química , Antioxidantes/química , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Catalase/metabolismo , Feminino , Glutationa/metabolismo , Glutationa Redutase/metabolismo , Coração/efeitos dos fármacos , Malondialdeído/metabolismo , Estrutura Molecular , Miocárdio/metabolismo , Compostos Organosselênicos/síntese química , Compostos Organosselênicos/química , Ratos , Ratos Wistar , Superóxido Dismutase/metabolismoRESUMO
Chemical toxic pollutants (especially heavy metals) are important sources of reactive oxygen species (ROS) in biological systems. Membrane phospholipids of aerobic organisms are continually subjected to oxidant challenges from endogenous and exogenous sources, while peroxidized membranes and lipid peroxidation products represent constant threats to aerobic cells. The primary antioxidant protection against free radical and ROS is provided by the enzymes glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT), respectively. The trace element selenium has been implicated in chemo-prevention and drug-resistance through reduction of oxidative stress. Selenium could prevent damage to the unsaturated fatty acid of subcellular membranes by lipid peroxidation induced by free radicals. The results reported here show that sodium selenite has an important contribution to antioxidative defense for the spleen and heart of rainbow trout. The ability of sodium selenite to prevent the oxidative stress induced by heavy metals (Cd(2+), Cr(3+)) in fish was rationalized.