[A study of the safety of probiotic bacilli].

The aim of the study was to evaluate the biological safety of a range of probiotic bacilli, ingredients of the commercial preparations biosporin, bactisubtil, and irilis; their biological effects were scrutinized in an animal experiment. The study demonstrated that the strains B. subtilis 3, 07, and B. licheniformis 31, 09, ingredients of biosporin and irilis, unlike B. cereus IP 5832, an ingredient of bactisubtil, were not toxic, toxigenic, or virulent, and did not impair the antitoxic hepatic function, which allows considering them to be safe.

[Pre-clinical trials of new spore probiotics].

The paper presents the results of a pre-clinical trial of Bacillus bacterial strains that are ingredients of biosporin, bisporin, and irilis, new Russian probiotic preparations. The study included determination of the cultural and biochemical properties of the probiotic strains, their antagonistic activity, adhesion ability, resistance to various biological liquids, and the length of their persistence in mouse alimentary tract. The listed properties were compared with those of foreign commercial preparations, bactisubtil and enterogermin. The study demonstrated high antagonistic activity of biosporin, bisporin, and irilis against many bacteria of different taxonomic groups, and Candidae. Besides this, the study showed that, unlike similar foreign preparations, the Russian ones were characterized by relatively low adhesive ability, short-term persistence, and absence of virulence factors. According to the results of the study, the most prospective probiotic strains for further clinical application are B. subtilis VKPM B-8611 and B. licheniformis VKPM B-8610, an irilis ingredient.

[Spore probiotics]. / Sporovye probiotiki.

Data on probiotic preparations prepared from spore-forming bacteria is reviewed. Information on indications for use, effectiveness and the mechanism of action is given. Preparations being produced in Russia (Biosporin, Sporobacterin and Bactosporin) are evaluated in detail as well as the prospects of the application of probiotics from spore-forming bacteria.

Combined effect of low-frequency laser and polyphenol oxidase on adhesive activity of Escherichia coli strains isolated from patients with urinary diseases.

The effects of polyphenol oxidase and low-frequency laser irradiation on adhesion of pathogenic Escherichia coli to human erythrocytes and buccal epithelial cells were studied. The maximum decrease in adhesive activity of these strains was observed after complex exposure to laser and enzyme.

Complex effects of pulsed infrared laser and asparaginase on Escherichia coli strains isolated from patients with urinary diseases.

Treatment with L-asparaginase and low-frequency laser decreased adhesion of uropathogenic Escherichia coli to human erythrocytes. The maximum effect was observed after combined treatment (laser exposure followed by enzyme treatment).

[Safety of bacteria of the genus Bacillus, forming the base of some probiotics]. / Izuchenie bezopasnosti bakterii roda Bacillus, sostavliaiushchikh osnovu nekotorykh probiotikov.

The study of the safety of bacillary strains forming the base of new probiotics (biosporin and subalin) was made. For control, the safety of Bacillus sp. IP5832 (the base of the preparation of bactisubtil) was studied. The results obtained in this investigation demonstrated that the strains contained in biosporin and subalin were safe when injected intravenously and intraperitoneally into animals in dose of 5 x 10(9) cells per 0.5 ml of physiological saline. The introduction of Bacillus sp. IP5832 in the same dose produced 100% lethal effect.