Unlocking the potential of microalgae cultivated on wastewater combined with salinity stress to improve biodiesel production.

Microalgae have the potential as a source of biofuels due to their high biomass productivity and ability to grow in a wide range of conditions, including wastewater. This study investigated cultivating two microalgae species, Oocystis pusilla and Chlorococcus infusionum, in wastewater for biodiesel production. Compared to Kühl medium, KC medium resulted in a significant fold increase in cellular dry weight production for both O. pusilla and C. infusionum, with an increase of 1.66 and 1.39, respectively. A concentration of 100% wastewater resulted in the highest growth for O. pusilla, with an increase in biomass and lipid content compared to the KC medium. C. infusionum could not survive in these conditions. For further increase in biomass and lipid yield of O. pusilla, different total dissolved solids (TDS) levels were used. Maximum biomass and lipid productivities were achieved at 3000 ppm TDS, resulting in a 28% increase in biomass (2.50 g/L) and a 158% increase in lipid yield (536.88 mg/g) compared to KC medium. The fatty acid profile of O. pusilla cultivated on aerated wastewater at 3000 ppm TDS showed a high proportion of desirable saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) for biodiesel production. Cultivating microalgae in wastewater for biodiesel production can be cost-effective, especially for microalgae adapted to harsh conditions. It could be concluded that O. pusilla is a promising candidate for biodiesel production using wastewater as a growth medium, as it has high biomass productivity and lipid yield, and its fatty acid profile meets the standard values of American and European biodiesel standards. This approach offers a sustainable and environmentally friendly solution for producing biofuels while reducing the environmental impact of wastewater disposal.

Nanobiotechnological advancements in agriculture and food industry: Applications, nanotoxicity, and future perspectives.

The high demand for sufficient and safe food, and continuous damage of environment by conventional agriculture are major challenges facing the globe. The necessity of smart alternatives and more sustainable practices in food production is crucial to confront the steady increase in human population and careless depletion of global resources. Nanotechnology implementation in agriculture offers smart delivery systems of nutrients, pesticides, and genetic materials for enhanced soil fertility and protection, along with improved traits for better stress tolerance. Additionally, nano-based sensors are the ideal approach towards precision farming for monitoring all factors that impact on agricultural productivity. Furthermore, nanotechnology can play a significant role in post-harvest food processing and packaging to reduce food contamination and wastage. In this review, nanotechnology applications in the agriculture and food sector are reviewed. Implementations of nanotechnology in agriculture have included nano- remediation of wastewater for land irrigation, nanofertilizers, nanopesticides, and nanosensors, while the beneficial effects of nanomaterials (NMs) in promoting genetic traits, germination, and stress tolerance of plants are discussed. Furthermore, the article highlights the efficiency of nanoparticles (NPs) and nanozymes in food processing and packaging. To this end, the potential risks and impacts of NMs on soil, plants, and human tissues and organs are emphasized in order to unravel the complex bio-nano interactions. Finally, the strengths, weaknesses, opportunities, and threats of nanotechnology are evaluated and discussed to provide a broad and clear view of the nanotechnology potentials, as well as future directions for nano-based agri-food applications towards sustainability.

Screening of seaweeds for sustainable biofuel recovery through sequential biodiesel and bioethanol production.

The present study evaluated the sequential biodiesel-bioethanol production from seaweeds. A total of 22 macroalgal species were collected at different seasons and screened based on lipid and carbohydrate contents as well as biomass production. The promising species was selected, based on the relative increase in energy compounds (REEC, %), for further energy conversion. Seasonal and annual biomass yields of the studied species showed significant variations. The rhodophyte Amphiroa compressa and the chlorophyte Ulva intestinalis showed the highest annual biomass yield of 75.2 and 61.5 g m-2 year-1, respectively. However, the highest annual carbohydrate productivity (ACP) and annual lipid productivity (ALP) were recorded for Ulva fasciata and Ulva intestinalis (17.0 and 3.0 g m-2 year-1, respectively). The later was selected for further studies because it showed 14.8% higher REEC value than Ulva fasciata. Saturated fatty acids (SAFs) showed 73.4%, with palmitic acid as a dominant fatty acid (43.8%). Therefore, biodiesel showed high saturation degree, with average degree of unsaturation (ADU) of 0.508. All the measured biodiesel characteristics complied the international standards. The first route of biodiesel production (R1) from Ulva intestinalis showed biodiesel recovery of 32.3 mg g-1 dw. The hydrolysate obtained after saccharification of the whole biomass (R2) and lipid-free biomass (R3) contained 1.22 and 1.15 g L-1, respectively, reducing sugars. However, bioethanol yield from R3 was 0.081 g g-1 dw, which represented 14.1% higher than that of R2. Therefore, application of sequential biofuel production using R3 resulted in gross energy output of 3.44 GJ ton-1 dw, which was 170.9% and 82.0% higher than R1 and R2, respectively. The present study recommended the naturally-grown Ulva intestinalis as a potential feedstock for enhanced energy recovery through sequential biodiesel-bioethanol production.

Production and characterization of antimicrobial active substance from the cyanobacterium Nostoc muscorum.

In this investigation, the cyanobacterium Nostoc muscorum exhibited antagonistic activity against Gram-positive and Gram-negative bacteria and filamentous fungi. The results indicated that the active substance produced maximally after 12 days of incubation in shaken culture at 35°C, at pH 8.0 in BG-11 medium. The increase in nitrate concentration of the medium led to an increase in the antimicrobial production. Chloroform was the best solvent for extracting the active material. The antagonistic material was purified using thin layer chromatography. The compound showed maximum absorption at 240nm. Infrared (IR) and nuclear magnetic resonance (NMR) indicated presence of γOH, γCH aromatic, γCH aliphatic, γCN, γCO, γCC and CO. Mass spectroscopy indicated that its molecular weight is 279. The results also indicated that the compound is phenolic compound.

Growth and heavy metals removal efficiency of Nostoc muscorum and Anabaena subcylindrica in sewage and industrial wastewater effluents.

The growth of Nostoc muscorum and Anabaena subcylindrica in sterilized sewage wastewater and N. muscorum in sterilized wastewater of El-Soda Company was higher than those grown in Allen synthetic medium. Whereas, the growth of A. subcylindrica in El-Soda Company sterilized wastewater and N. muscorum as well as A. subcylindrica grown in Verta Company sterilized wastewater was slightly lower than that grown in the standard synthetic medium. The contents of chlorophyll a, carotenoids and protein of N. muscorum and A. subcylindrica grown in sterilized sewage wastewater were higher than those grown in the standard medium. Similarly, N. muscorum and the bio-mixture of N. muscorum and A. subcylindrica grown in the sterilized wastewater of El-Soda Company showed high pigments and protein contents more than those reared in Allen medium. On the other hand, the bio-mixture of N. muscorum and A. subcylindrica grown in the sterilized sewage wastewater, A. subcylindrica grown in El-Soda Company and Verta Company sterilized wastewater showed lower contents of pigments and protein compared to synthetic medium. Heavy metals, copper, cobalt, lead and manganese were removed by 12.5-81.8, 11.8-33.7, 26.4-100 and 32.7-100%, respectively, from wastewater by using cyanobacterial cultures. The metal sorption efficiency depended on the type of biosorbent, the physiological state of the cells, availability of heavy metal, concentration of heavy metal and chemical composition of wastewater. It was observed also that the single cultures in most cases was better than the mixed cultures in heavy metal removal, this may be due to the cyanobacterial competition for nutrients in mixed cultures.

Differential effects of Co(2+) and Ni(2+) on protein metabolism in Scenedesmus obliquus and Nitzschia perminuta.

Growth, morphological changes, amino acid composition, total soluble protein, and protein electrophoretic pattern were monitored for Scenedesmus obliquus and Nitzschia perminuta grown in the presence of different concentrations of Co(2+) and Ni(2+). Lower concentrations of cobalt stimulated the dry mass production and total soluble protein content of the two algae, whereas higher concentrations were inhibitory. Generally, N. perminuta showed more tolerance to the phytotoxicity of the two metals than S. obliquus and more tolerance to nickel than cobalt. However, S. obliquus seems to be more tolerant to cobalt than nickel. Cobalt and nickel have induced an increase in cell volume, change and disorder in cell shape. The increase in cell volume was much observed in Ni(2+) treated cells. At the same time, the two metals did not induce any distinct morphological abnormalities in N. perminuta. Co(2+) has stimulated the biosynthesis of all free amino acids in S. obliquus, except aspartic acid and phenylalanine, whereas Ni(2+) caused 22% inhibition in the content of total free amino acids, except cystine and arginine. On the other hand, Co(2+) has reduced the content of free amino acids in N. perminuta, except cystine, methionine, valine, and lysine. On the other hand, Ni(2+) stimulated the biosynthesis of glycine, alanine and histidine and highly stimulated valine and sulphur containing amino acids (cystine and methionine) in N. perminuta. High cobalt concentration (4ppm) resulted in the disappearance of 28.7kDa protein, 3.5ppm Ni(2+) stimulated the appearance of 18 and 20kDa proteins in S. Obliquus, while 37kDa proteins disappeared from N. perminuta treated with high doses of Co(2+) and Ni(2+).