Assessment of clinical effects and safety of an oral supplement based on marine protein, vitamin C, grape seed extract, zinc, and tomato extract in the improvement of visible signs of skin aging in men.

BACKGROUND: Skin aging is a natural process that may be aggravated by environmental factors. Topical products are the conventional means to combat aging; however, the use of oral supplements is on the rise to assist in the management of aged skin. OBJECTIVE: The purpose of this study was to assess the effects and safety of an oral supplement containing (per tablet) marine protein (105 mg), vitamin C (27 mg), grape seed extract (13.75 mg), zinc (2 mg), and tomato extract (14.38 mg) in the improvement of skin aging in men. METHODS: This single-center, open-label, quasi-experimental clinical study enrolled 47 male subjects, aged 30-45 years, with phototypes I-IV on the Fitzpatrick scale. Subjects received two tablets of the oral supplement for 180 consecutive days. Each subject served as their own control. Clinical assessments were made by medical personnel and by the subjects, respectively. Objective assessments were carried out through pH measurements, sebumetry, corneometry, ultrasound scanning, skin biopsies, and photographic images. RESULTS: Forty-one subjects (87%) completed the study. Clinical improvements on both investigator- and subject-rated outcomes were found for the following parameters: erythema, hydration, radiance, and overall appearance (P<0.05). The objective measurements in the facial skin showed significant improvements from baseline in skin hydration (P<0.05), dermal ultrasound density (P<0.001), and reduction of skin pH (P<0.05). No statistical improvement in relation to sebum was found. The photographic assessment showed an improvement in the overall appearance. The results of the objective measurements were found to be correlated with the subjects' satisfaction by an increase of collagen and elastic fibers. CONCLUSION: The use of an oral supplement based on a unique biomarine complex, vitamin C, grape seed extract, zinc, and tomato extract produced improvements in the signs of skin aging in men.

Eficácia in vitro e clínica do uso cosmético tópico de fitoestrógenos em pele fotoenvelhecida / In vitro and clinical efficacy of the use of phytoestrogens-based topical cosmetic use in photoaged skin

Introdução: O tratamento do envelhecimento cutâneo representa um desafio clínico. Objetivos: Avaliar a eficácia in vitro e in vivo, e a segurança clínica de cosmético com fitoestrógenos na abordagem do fotoenvelhecimento. Métodos: A etapa in vitro foi realizada pela análise da expressão gênica de fibronectina e pró-colágeno, avaliação da atividade imunomoduladora e análise histoquímica e por imunofluorescência da pele e da junção dermoepidérmica com o produto analisado. No estudo clínico in vivo foi 76 mulheres, foram randomizadas em dois grupos: o Grupo A usou creme contendo complexo de fitoestrógenos e FPS 20 duas vezes ao dia, enquanto o Grupo B usou este mesmo creme associado a outro com função de antienvelhecimento aplicado à noite. O estudo durou 120 dias tendo sido realizadas mensalmente avaliações médicas, da voluntária, ultrassonografia (20MHz), fotografias e biópsias pré e pós-tratamento. Resultados: No estudo in vitro houve aumento na expressão de fibronectina e procolágeno, potencial imunomodulador, representado pelo aumento de IL-1α diminuição de IL-10; melhora da integridade da JDE, aumento da viabilidade e espessura da epiderme, e da síntese de colágeno. in vivo: melhora global subjetiva da aparênciada pele da face; redução de manchas, eritema, poros e porfirina cutânea. O ultrassom e a biópsia revelaram aumento da densidade dérmica (52,7%) e de fibras dérmicas (22,25%), respectivamente. Conclusões: Fitoestrógenos tópicos melhoram a condição geral da pele, avaliada clínicamente, histológicamente e por ultrassonografia; acrescentam-se resultados in vitro de aumento da síntese de fibronectina, prócolágeno e colágeno, melhoria da integridade da junção dermoepidérmica e restauração da resposta imunológica da pele.

Introdução: Introduction: Skin aging is a challenge to treat. Objectives: To evaluate the in vitro and in vivo efficacy and clinical safety of a phytoestrogens-based cosmetic in the management of photoaging. Methods: The in vitro investigation was performed using the analysis of the genic expression of fibronectin and procollagen, evaluation of the immunomodulatory activity (proinflammatory and anti-inflammatory cytokines synthesis) and histochemistry and immunofluorescence analyses of the skin and the dermoepidermal junction. The in vivo investigation ­ performed in 76 women randomized into Group A (phytoestrogens complex cream and SPF 20 twice daily) or Group B (the same product plus a commercially available anti-aging cream applied overnight). The study lasted for 120 days, with physician- and patient-led evaluations, in addition to monthly ultrasound (20 MHz) and photographic analysis. Skin biopsies of the face were performed before and after treatment. Results: The study showed In vitro: increase in the expression of fibronectin, in procollagen, immunomodulator potential, represented by an increase in IL-1α and a decrease in IL-10; improvement in the integrity of the dermoepidermal junction; increase in the viability and thickness of the epidermis; increase in collagen synthesis .In vivo: subjective global improvement of the skin's appearance; reduction in the count and intensity of spots, erythema, skin pores, and cutaneous porphyrin. The ultrasound and biopsy revealed increased dermal density (52.7%) and dermal fibers (22.3%), respectively. Conclusions: The topical use of phytoestrogens-based cosmetics improves the overall condition of the skin.

Clinical, biometric and ultrasound assessment of the effects of daily use of a nutraceutical composed of lycopene, acerola extract, grape seed extract and Biomarine Complex in photoaged human skin.

BACKGROUND: The use of nutraceuticals has become frequent in the cutaneous approach to photoaging. OBJECTIVES: To assess the clinical efficacy of a nutraceutical product composed of lycopene, acerola extract, grape seed extract and Biomarine ComplexT in photoaged human skin. METHODS: 50 women, from 35 to 60 years of age, phototypes I to III, were assessed. For 120 days, they associated the nutraceutical product with the use of a sunscreen FPS15. On days 0 (D0), 30 (D30), 60 (D60), 90 (D90) and 120 (D120) they were evaluated and underwent Medical Assessments and Self-Assessment and cutaneous biometric analyses (corneometry, sebumetry and pH-metry) in the skin of the left zygomatic region and the upper medial side region of the left arm; on days 0 (D0), 30 (D30) and 120 (D120) the skin of the same regions was analyzed by ultrasound. On days 0 (D0) and 120 (D120) skin biopsies were performed in the areas where instrumental evaluation was performed (to evaluate collagen and elastic fibers). RESULTS: There was an improvement of the general status of the skin of all volunteers by the Medical and Volunteer Self- Assessments; increased parameters of cutaneous hydration, reduction of pH, increasing of ultrasound density and a histological increment of collagen and elastic fibers (both on the face and arm); there was a reduction of seborrhea (only on the face) CONCLUSIONS: The daily use of a nutraceutical product containing lycopene, acerola extract, grape seed extract and Biomarine ComplexT showed an important adjuvant effect to counteract skin photoaging.

Clinical, biometric and ultrasound assessment of the effects of daily use of a nutraceutical composed of lycopene, acerola extract, grape seed extract and Biomarine Complex in photoaged human skin / Avaliações clínica, biométrica e ultrassonográfica dos efeitos do uso diário de um nutracêutico a base de licopeno, extrato de acerola, extrato de semente de uva e Complexo Biomarinho na pele fotoenvelhecida humana

BACKGROUND: The use of nutraceuticals has become frequent in the cutaneous approach to photoaging. OBJECTIVES: To assess the clinical efficacy of a nutraceutical product composed of lycopene, acerola extract, grape seed extract and Biomarine ComplexT in photoaged human skin. METHODS: 50 women, from 35 to 60 years of age, phototypes I to III, were assessed. For 120 days, they associated the nutraceutical product with the use of a sunscreen FPS15. On days 0 (D0), 30 (D30), 60 (D60), 90 (D90) and 120 (D120) they were evaluated and underwent Medical Assessments and Self-Assessment and cutaneous biometric analyses (corneometry, sebumetry and pH-metry) in the skin of the left zygomatic region and the upper medial side region of the left arm; on days 0 (D0), 30 (D30) and 120 (D120) the skin of the same regions was analyzed by ultrasound. On days 0 (D0) and 120 (D120) skin biopsies were performed in the areas where instrumental evaluation was performed (to evaluate collagen and elastic fibers). RESULTS: There was an improvement of the general status of the skin of all volunteers by the Medical and Volunteer Self- Assessments; increased parameters of cutaneous hydration, reduction of pH, increasing of ultrasound density and a histological increment of collagen and elastic fibers (both on the face and arm); there was a reduction of seborrhea (only on the face) CONCLUSIONS: The daily use of a nutraceutical product containing lycopene, acerola extract, grape seed extract and Biomarine ComplexT showed an important adjuvant effect to counteract skin photoaging.

FUNDAMENTOS: O uso de nutracêuticos se tornou uma condição frequente na abordagem cutânea do fotoenvelhecimento. OBJETIVOS: Avaliar a eficácia clínica do uso de um produto nutracêutico a base de licopeno, extrato de acerola, extrato de semente de uva e Complexo BiomarinhoT na pele fotoenvelhecida humana. MÉTODOS: Foram avaliadas 50 mulheres, de 35 a 60 anos de idade, fototipo I a III. Por 120 dias, elas associaram ao uso de fotoprotetor FPS15 a tomada diária do produto nutracêutico em questão. Nos dias 0 (D0), 30 (D30), 60 (D60), 90 (D90) e 120 (D120) elas foram avaliadas, quando sofreram Avaliações Médica e Auto-Avaliação e análises biométricas cutâneas (corneometria, sebumetria e pHmetria) nas peles da região zigomática esquerda e face súpero-medial do braço esquerdos; nos dias 0 (D0), 30 (D30) e 120 (D120) a pele das mesmas regiões foram analisadas do ponto de vista ultrassonográfico. Nos dias 0 (D0) e 120 (D120) biópsias cutâneas foram realizadas nas respectivas áreas das análises instrumentais (para avaliar colágeno e das fibras elásticas). RESULTADOS: Houve melhora do estado geral da pele de todas as voluntárias pelas Avaliações Médica e Voluntária; aumento alterações dos parâmetros cutâneos na hidratação, redução do pH cutâneo, aumento da densidade ultrassonográfica e aumento histológico na densidade colágena e elástica (tanto na face quanto no braço); redução da seborreia somente na face. CONCLUSÕES: O uso diário de um produto nutracêutico a base de licopeno, extrato de acerola, extrato de semente de uva e Complexo BiomarinhoT mostra-se um adjuvante importante na abordagem do fotoenvelhecimento cutâneo.

Resultado de 360 das de uso de suplemento oral à base de proteína marinha, acerola concentrada, extrato de semente de uva e extrato de tomate em mulheres posrtadoras de envelhecimento cutâneo / Treating cutaneous aging in women, with an oral supplement based on marine protein, concentrated acerola, grape seed extract and tomato extract, within 360 days

Introdução: O envelhecimento cutâneo atinge ou atingirá todas as pessoas, e seu tratamento representa um desafio clínico. Objetivo: Avaliar efeitos cutâneos do uso de um suplemento oral a base de proteína marinha, acerola concentrada e extratos de semente de uva e tomate por 360 dias em portadoras de fotoenvelhecimento cutâneo. Métodos: Quarenta e cinco voluntárias usaram dois comprimidos diários do referido suplemento por 360 dias consecutivos. Avaliações clínicas (por parte dos investigadores e das voluntárias), ultrassonográficas e fotográficas foram realizadas a cada 30 dias. Resultados: Trinta e três voluntárias (73,3%) concluíram o estudo. A melhora clínica foi evidenciada pelos investigadores e voluntárias após 30 dias de uso do suplemento oral, o que resultou em ganho estatístico ao longo do tempo (p<0,05). Após 330 dias, constatou-se a tendência à estabilização dos resultados (p=0,05). Pela análise ultrassonográfica, percebeu-se aumento na densidade dérmica das áreas fotoexpostas (132,3%; p<0,001) e das fotoprotegidas (51,9%; p=0,001). Encontraram-se melhorias estatisticamente significativas nos quesitos rugas, linhas finas, melanoses solares, outras hipercromias, eritema, hidratação, viço, oleosidade, suavidade ao toque e aparência geral da pele através da análise médica; segundo a autoavaliação das voluntárias, obteve-se melhoria no padrão das rugas, linhas finas, melanoses solares, outras hipercromias, eritema, hidratação, viço, oleosidade, suavidade ao toque e aparência geral da pele. Conclusão: O uso de suplementação oral à base de proteína marinha, acerola concentrada e extratos de semente de uva e tomate por longo prazo (360 dias) mostra-se boa abordagem sistêmica adjuvante para o fotoenvelhecimento cutâneo.

Introduction: Cutaneous aging affects (or will affect) all people at some point in their life, and its treatment represents a clinical challenge. Objective: To evaluate the cutaneous effects of an oral supplement based on marine protein, concentrated acerola and extracts of grape seed and tomato in women with cutaneous aging. Methods: Forty-five volunteers used 2 daily tablets of the supplement for 360 consecutive days. Clinical evaluations (carried out by both the investigator physicians and by the volunteers), ultrasonographic and photographic examinations were carried out every 30 days. Results: Thirty-three volunteers (73.3%) completed the study. Clinically significant improvement was verified by the investigator physicians and the volunteers after 30 days of using the oral supplement (p < 0.05). The results were maintained after 330 days (p ¿ 0.05). The ultrasonographic analysis demonstrated increases in the dermal density of the photoexposed (132.3%; p < 0.001) and photoprotected areas (51.9%; p ¿ 0.001). Through medical analysis, statistically significant improvements were found in the following criteria: wrinkles, fine lines, solar melanoses, other hyperchromias, erythema, hydration, radiance , sebum, smoothness and overall appearance of the skin. Through thevolunteers' self-evaluation, improvements in the wrinkles' pattern, fine lines, solar melanoses, other hyperchromias, erythema, hydration, radiance, sebum, smoothness and overall appearance of the skin were reported. Conclusion: The long-term use (360 days) of an oral supplement based on marine protein, concentrated acerola andextracts of grape seed and tomato was proven to be a good adjuvant systemic approach for treating cutaneous aging.

Peeling de gel de ácido tioglicólico 10%: opção segura e eficiente na pigmentação infraorbicular constitucional / 10% thioglycolic acid gel peels: a safe and efficient option in the treatment of constitutional infraorbital hyperpigmentation

Introdução: A hipercromia infraorbicular constitucional é dermatose comum, de difícil tratamento. Objetivo: Avaliar a melhoria clínica da pigmentação infraorbicular constitucional com cinco peelings seriados de ácido tioglicólico a 10% em gel. Métodos: 10 voluntárias do sexo feminino, entre 24 e 50 anos de idade, realizaram cinco sessões quinzenais de peeling de ácido tioglicólico 10% gel. Na primeira sessão, o produto foi deixado por dois minutos, acrescentando-se três minutos a cada uma das sessões subsequentes, tendo na última sido deixado por 15 minutos; 15 dias após a última sessão, foi aplicada escala de satisfação clínica, de 0 (ausência de melhora) a 10 (melhora total), tanto às pacientes quanto ao médico aplicador e a um médico avaliador-cego. Resultados: A média da satisfação clínica apontada pelas pacientes foi 7,8; a do médico aplicador, 7,6; e a do médico avaliador-cego, 6,8, sem diferenças estatísticas entre eles (p=0,065). Conclusão: Os peelings seriados de ácido tioglicólico 10% em gel são alternativa segura, eficiente e barata para a abordagem da pigmentação infraorbicular constitucional.

Introduction: Constitutional infraorbital hyperchromia (commonly called under-eye circles) is a common dermatosis that is difficult to treat. Objective:To evaluate the clinical improvement of constitutional infraorbital pigmentation resulting from the application of a series of five 10% thioglycolic acid gel peeling sessions. Methods: Patients were administered five successive 10% thioglycolic acid gel peel sessions, in 15- day intervals. In the first session, the product was left on for two minutes, with three minutes added in each subsequent session, culminating with 15 minutes of contact in the last session. Fifteen days after the last session, a satisfaction scale ranging from 0 (lack of improvement) to 10 (total improvement) was administered to the patients, to the dermatologist investigating the peelings, and to a blindevaluator medical doctor (a dermatologist that had not had taken part in the treatment sessions). Results: Study subjects (n=10) were females aged 24-50.The average clinical satisfaction presented by the patients, the investigator, and the blind-evaluator were 7.8, 7.6 and 6.8, respectively, with no statistically significant differences among them (p=0.065). Conclusion: The serial application of 10% thioglycolic acid gel peels is a safe, efficient, and costeffective treatment for constitutional infraorbital pigmentation.