Validation of locomotion scoring as a new and inexpensive technique to record circadian locomotor activity in large mammals.

BACKGROUND: The locomotor activity (LA) rhythm, widely studied in rodents, has not been fully investigated in large mammals. This is due to the high cost and the brittleness of the required devices. Alternatively, the locomotion scoring method (SM), consisting of attribution of a score to various levels of activity would be a consistent method to assess the circadian LA rhythm in such species. NEW METHOD: To test this, a SM with a score ranging from 0 to 5 has been developed and used in two domestic large mammals, the camel and the goat. One minute interval scoring was performed using visual screening and monitoring of infra-red camera recording videos and carried out by two evaluators. RESULTS: The SM provides a clear daily LA rhythm that has been validated using an automate device, the Actiwatch-Mini. The obtained curves and actograms were indeed highly similar to those acquired from the Actiwatch-Mini. Moreover, there were no statistical differences in the period and acrophase. The period was exactly of 24.0h and the acrophases occurred at 12h05 ± 00h03 and 12h14 ± 00h07 for the camel and at 13h13 ± 00h09 and 12h57 ± 00h09 for the goat using SM and Actiwatch-Mini respectively. COMPARISON WITH EXISTING METHODS: Compared to the automatic system, the SM is inexpensive and has the advantage of describing all types of performed movements. CONCLUSIONS: The new developed SM is highly reliable and sufficiently accurate to assess conveniently the LA rhythm and specific behaviors in large mammals. This opens new perspectives to study chronobiology in animal models of desert, tropical and equatorial zones.

Estimation of the liveweight of working mules in Morocco from their body measurements.

During March 2001, 117 working mules, randomly chosen from four markets in Morocco, were weighed and a series of body measurements recorded; their age and body condition score were also recorded. Linear regression techniques were used to derive a 'best fit' equation for predicting liveweight from the other variables. For the working mules weighing between 131 and 391 kg the best prediction equation using two variables was: liveweight (kg) = -33 + 2.8 x heart girth (cm)+1.36 x length (cm). Other prediction equations available for estimating the liveweight of equidae were tested on the data, but in all cases they significantly overestimated the weights of the Moroccan mules.

Regeneration of neurosecretory axons into various types of intrahypothalamic grafts is promoted by the absence of blood brain barrier: fine structural analysis.

Isogenous grafts of neural lobe and optic nerve and autologous grafts of sciatic nerve were placed into contact with the intrahypothalamically transected hypothalamo-neurohypophysial tract, and their fine structural characteristics examined at various time periods thereafter. The vascular bed of neural lobe grafts is composed primarily of fenestrated capillaries, that are permeable to blood-borne HRP throughout the entire experimental period. The microvasculature of sciatic nerve grafts consists of continuous, as well as fenestrated capillaries, which are similarly permeable to HRP. Fenestrated capillaries and HRP leakage in optic nerve grafts are observed at 10 days, but only in grafts located ventrally in the hypothalamus at 30 days. Neurosecretory axon regeneration is seen only in grafts or adjacent hypothalamus where the blood-brain barrier is breached. Regenerating axons are closely associated with the specific glial cells of the respective graft. Based on these observations, we conclude that blood-borne factors are necessary to initiate and sustain regeneration of transected neurosecretory axons, and that such regeneration occurs only in the presence of glial cells.

Regeneration of neurosecretory axons into various types of intrahypothalamic graft is promoted by the absence of the blood-brain barrier: a neurophysin-immunohistochemical and horseradish peroxidase-histochemical study.

In order to test the hypothesis that neurosecretory axon regeneration occurs only in the presence of specific vascular, perivascular, and glial microenvironments, isografts of neural lobe and optic nerve and autografts of sciatic nerve were transplanted into the hypothalamo-neurohypophysial tract at the lateral retrochiasmatic area of adult male rats. The integrity of the blood-brain barrier (BBB) to intravenously administered horseradish peroxidase (HRP), the regenerative process of neurosecretory axons, and functional recovery from lesion-induced diabetes insipidus were analyzed at 18 hr, 36 hr, 10 days, 30 days, and 80 days postsurgery. Neurophysin-positive axons invaded all grafts, as well as perivascular spaces of the adjacent hypothalamus. Wherever neurosecretory axon regeneration occurred, the BBB was breached. Reestablishment of the BBB was paralleled by a decrease in both density and staining intensity of regenerated neurophysin-positive axons. These observations illustrate that neurosecretory axon regeneration is tributary of the absence of BBB. It is speculated that blood-borne factors, provided when the BBB is breached, initiate and sustain neurosecretory axon regeneration. In addition, products of glial elements may enhance or complement the above stimulatory processes.

Estimation of the liveweight and body condition of working donkeys in Morocco.

The age, sex, liveweight and body measurements (heart girth, umbilical girth, height, length from elbow to tuber ischii and circumference of the foreleg cannon bone) of 516 donkeys used to transport goods in Morocco were recorded. There were few donkeys over 12 years of age. For adult donkeys weighing from 74 to 252 kg, the best equation with only one variable for predicting liveweight was: liveweight (kg) = heart girth (cm)2.65/2188. The inclusion of two variables improved the prediction marginally, but the addition of further variables gave little further improvement. The best prediction equation for adult donkeys was: liveweight (kg) = (heart girth [cm]2.12 x length [cm]0.688)/380. For donkeys under three years of age, weighing from 52 to 128 kg, the best prediction equation was: liveweight (kg) = (umbilical girth [cm]1.41 x length [cm]1.09)/1000. Other liveweight prediction equations for donkeys and horses were tested on the data and tended to overestimate the weight of these working donkeys. A subjective method for assessing the body condition of the donkeys was developed, using a scale from 1 (emaciated) to 9 (obese).

An immunohistochemical and fine-structural analysis of peptidergic hypothalamic neurosecretory axon regeneration into the leptomeninges of the rat.

Regeneration of severed hypothalamic peptidergic neurosecretory axons into the ventral pia-arachnoid was observed in rats at the light microscopic and fine-structural levels. A temporal increase occurred in the number of neurophysin-positive axons regenerating into the leptomeninges for distances up to 3.3 mm by 40 days post-lesioning. A consistent pattern of parallel, meshed and clustered axons, occurring either singly or in bundles, was present within the connective tissue, while plexus and bundles were observed in association with leptomeningeal blood vessels. Axons were characterized by preterminal and terminal dilatations. Neurosecretory granulated vesicles occurred throughout axons. The presence of microvesicles at contact points with basal lamina suggests the possibility of hormone release. Most axons were arranged as fascicles associated closely with basal lamina-bounded support cells whose thin lamellar processes wrapped single axons or fascicles of axons. We conclude, therefore, that cellular and intercellular leptomeningeal microenvironments support and sustain the growth and regeneration of transected neurosecretory axons.