Relationships between age, body measurements, and testicular measurements in Arabia bucks in Algeria.

Body size and testicular measurements were found to be important parameters for the evaluation of the breeding quality. A study was therefore carried out on Arabia bucks (n = 180) to record normal testicular characteristics and to evaluate the effect of age on body (BCS, BW, WH, CG, AC, CS, and BL) and testicular (SC, TW, EW, TL, TD, and TL) measurements. Animals were classified into three age classes (less than or equal to 12 months, from 12 to 24 months, and over 24 months). The BW, BCS, testicular, and body measurements of the 3rd age group were significantly higher than those of age groups 1 and 2 (p < 0.01). Male age had a significant effect (p < 0.01) on BW, BCS, and body measurements. Indeed, the parameters of the 3rd age group were significantly higher than those of the 1st and 2nd age groups (p < 0.01). The same was true for testicular measurements (SC, TL, TW, EW, and EL) except for testicular diameter (TD) where the results of the first two age groups had no significant difference. The highest correlation coefficients were recorded between BW and all body (r = 0.7-0.9) and testicular traits (r = 0.72-0.85), BCS and body measurements (r = 0.73-0.89), and scrotal circumference and testicular measurements (r = 0.77-0.85). In conclusion, it was found that the use of BCS is readily measurable in live animals and is expected to be the best indicator of testicular and epididymal measurements. These results, particularly the predictive models developed, could be useful in the selection of males for reproduction.

Fasciolosis due to Fasciola hepatica in ruminants in abattoirs and its economic impact in two regions in Algeria.

and February 2009. The aim of the study was to determine the prevalence of infected livers with fascioliasis and to evaluate the economic loss due to the condemnation of livers infected with fascioliasis. A total of 3,457 cattle, 6,161 sheep and 5,764 goats were slaughtered and examined in El Tarf abattoir, north Algeria (humid climate), and 2,151 cattle, 5,724 sheep, 351 goats and 582 camels in Ouargla abattoir, south Algeria (saharan climate). In El Tarf region, the prevalence of fascioliasis infection was 26.7±2.5%, 6.5±0.4% and 2.5±0.2% in cattle, sheep and goats, respectively (P< 0.001). In Ouargla region, fascioliasis is reported for the first time in cattle (1.7±0.7%). Sheep, goats and camels were not infected. The overall economic loss due to hepatic condemnations as a result of fascioliasis was estimated at 60,000 euros in El Tarf region and 4,000 euros in Ouargla region. According to this study, it can be concluded that fascioliasis is endemic in the northern Algeria in ruminants and, the disease should be considered seriously as a great threat to ruminant health and profitability.

Prevalence of Fasciola hepatica in Galba truncatula detected by Multiplex PCR in the province of El Tarf (Algeria).

The prevalence of Fasciola hepatica infection in the snail Galba truncatula was studied for the first time in Algeria using Multiplex PCR. A total of 722 individuals were collected from 11 typical habitats (temporary ponds, road ditches and puddles) distributed in five localities of the El Tarf province, known for endemicity of fascioliasis. Snails were divided in 75 groups and DNA extraction was performed using Chelex® (Biorad, Nazareth Eke). Two primers were used, the first is specific for 124 bp DNA fragment of Fasciola sp and the second one represents the ITS-2 lymnaeids sequence (500-600 bp). The prevalence of the infection was estimated to be 46.66% for pools (35/75 were positive). The bursting out of the pools show that 75 snails were carriers of F. hepatica which gives an overall infection rate of about 10.74%. The results of snails infection according to their sizes showed significant differences in the studied size classes, thus snails of big sizes were the most infected. Based on these data, epidemiological implication of Multiplex PCR as a fully reliable technique to highlight high risks periods of fasciolosis will be of a great interest.

Prevalence of Fasciola hepatica in Galba truncatula detected by Multiplex PCR in the province of El Tarf (Algeria)

The prevalence of Fasciola hepatica infection in the snail Galba truncatula was studied for the first time in Algeria using Multiplex PCR. A total of 722 individuals were collected from 11 typical habitats (temporary ponds, road ditches and puddles) distributed in five localities of the El Tarf province, known for endemicity of fascioliasis. Snails were divided in 75 groups and DNA extraction was performed using Chelex® (Biorad, Nazareth Eke). Two primers were used, the first is specific for 124 bp DNA fragment of Fasciola sp and the second one represents the ITS-2 lymnaeids sequence (500–600 bp). The prevalence of the infection was estimated to be 46.66% for pools (35/75 were positive). The bursting out of the pools show that 75 snails were carriers of F. hepatica which gives an overall infection rate of about 10.74%. The results of snails infection according to their sizes showed significant differences in the studied size classes, thus snails of big sizes were the most infected. Based on these data, epidemiological implication of Multiplex PCR as a fully reliable technique to highlight high risks periods of fasciolosis will be of a great interest.