RESUMO
The wing spot test of Drosophila melanogaster was done to evaluate the genotoxicity of the antitumor indenoisoquinoline analogues of nitidine chloride and fagaronine chloride in larvae. Both compounds have toxic effects but no statistically significant increase in the frequency of spots was detected with the analogue of nitidine chloride. This strongly suggests that this compound is not mutagenic to Drosophila larvae at the concentrations tested. Results with the analogue of fagaronine chloride were ambiguous. Low mutagenicity was detected in only one of two experiments and in the pooled results at 2 mM but not at 5 mM or 10 mM. These results suggest at best a very weak genotoxic effect but its biological significance needs confirmation by results from other assays.
Assuntos
Alcaloides/toxicidade , Antineoplásicos/toxicidade , Drosophila melanogaster/efeitos dos fármacos , Isoquinolinas/toxicidade , Mutagênicos/toxicidade , Animais , Benzofenantridinas , Drosophila melanogaster/genética , Larva/efeitos dos fármacos , Testes de Mutagenicidade , Fenantridinas/toxicidadeRESUMO
The wing somatic mutation and recombination test (SMART) of Drosophila melanogaster was used to study the mutagenic potential of three benzo[c]phenanthridines with antileukemic properties, fagaronine, nitidine and O-methylfagaronine, as compared with that of two structurally related aromatic polycyclic hydrocarbons: 7,12-dimethylbenz[a]anthracene and chrysene. Although toxic to larvae, the benzo[c]phenanthridines and chrysene gave negative or inconclusive results while 7,12-dimethylbenz[a]anthracene was found to be highly mutagenic and recombinogenic as previously reported. These results suggest that the alkoxy groups and the quaternary nitrogen of the benzo[c]-phenanthridines may reduce or eliminate their mutagenicity in spite of their similarity to methylated polycyclic aromatic hydrocarbons.
Assuntos
Alcaloides/toxicidade , Antimutagênicos/química , Antineoplásicos Fitogênicos/toxicidade , Drosophila melanogaster/efeitos dos fármacos , Mutagênicos/toxicidade , Fenantridinas/toxicidade , 9,10-Dimetil-1,2-benzantraceno/química , 9,10-Dimetil-1,2-benzantraceno/toxicidade , Alcaloides/química , Animais , Benzofenantridinas , Crisenos/química , Crisenos/toxicidade , Drosophila melanogaster/genética , Feminino , Masculino , Mutagênese , Testes de Mutagenicidade , Mutagênicos/química , Fenantridinas/química , Recombinação Genética , Relação Estrutura-AtividadeRESUMO
The antitumor drug, 3-nitrobenzothiazolo[3,2-a]quinolinium chloride (NBQ) was tested for genotoxicity with the sex-linked recessive lethal test by feeding Drosophila melanogaster males. Although toxic to adults, the drug tested negative at the concentrations studied.
Assuntos
Antineoplásicos/toxicidade , Drosophila melanogaster/efeitos dos fármacos , Compostos de Quinolínio/toxicidade , Animais , Drosophila melanogaster/genética , Feminino , Genes Letais , Genes Recessivos , Masculino , Testes de Mutagenicidade/métodosRESUMO
Fagaronine, a potent antileukemic agent, was shown to induce chromosome breaks and loss in sperm of Drosophila melanogaster.