Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 23
Filtrar
Mais filtros










Intervalo de ano de publicação
1.
Anim Reprod ; 19(1): e20220018, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35493785

RESUMO

SexedULTRA-4M™ is made using an improved method of sex-sorting sperm in a less damaging environment for better retaining sperm integrity throughout the sorting process. The objective of this research was to compare conventional (CONV) and SexedULTRA-4M™ (ULTRA-4M) semen for bovine IVP using four Angus bulls. Matured slaughterhouse oocytes (n = 4000) were divided into the CONV group and the ULTRA-4M group (2000 COCs for each semen type). The IVF process was implemented with CONV and ULTRA-4M semen from the same bull. The cleavage rates, eight cell embryos and blastocysts on day 7 of culture were evaluated for each semen type and each bull. The statistical analysis was carried out with the ANOVA procedure SAS software. The results were 54.45% ± 1.03 and 58.10% ± 1.07; 35% ± 1.57 and 39.15% ± 1.62; 22.8% ± 1.09 and 27.15% ± 1.12 for CONV and ULTRA-4M, respectively, for cleavage rate, eight cell embryos and blastocysts on day 7 for the average of all bulls, comparing only the semen type. Concerning only the semen type, ULTRA-4M was significantly superior to CONV for cleavage rates (P = 0.01) and blastocysts on day 7 (P = 0.009). There were no significant differences between the CONV and ULTRA-4M groups (P>0.05) for all variables analyzed for Bull 1 and Bull 4, however, for Bull 2 ULTRA-4M was significantly superior to CONV for cleavage rates and blastocysts on day 7 (P< 0.05). In Bull 3, ULTRA-4M was significantly higher (P< 0.05) for blastocysts on day 7 compared to CONV. In conclusion, under the conditions of this research the ULTRA-4M and CONV semen produced similar bovine IVP results overall.

2.
Vet Ital ; 55(2): 183-187, 2019 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-31274182

RESUMO

The cuniculture has become an important source of animal protein in many countries. The coccidiosis is the most common parasitic disease of the rabbits and is responsible for severe economic losses for breeders. Rabbit coccidiosis is caused by 11 species of the genus Eimeria, which vary considerably in terms of their morphology and pathogenicity. The aim of this study was to evaluate prevalence of Eimeria spp in backyard farms from Mexico during annual seasons. Cross-sectional sampling was performed in young rabbits (20 to 60 days of age) with diarrhea history, from three municipalities located in the south-east region from the State of Mexico. Flotation and Mc Master techniques were performed; oocysts were sporulated and measured for morphometric identification. The highest prevalence of Eimeria was found in autumn (75%) in Temamatla and winter (88%) in Amecameca, being the lower prevalence in spring (5%) in Temamatla. In terms of their pathogenicity  E. itestinalis was the more pathogen found in this study, being the annual prevalence of 11.3%. It is important to continue with studies of prevalence in other regions of the State of Mexico, in order to understand the pattern of presentation and distribution of the Eimeria spp infection.


Assuntos
Coccidiose/veterinária , Eimeria/isolamento & purificação , Coelhos , Animais , Coccidiose/epidemiologia , Coccidiose/parasitologia , Estudos Transversais , México/epidemiologia , Prevalência , Estações do Ano
3.
Int. j. morphol ; 36(1): 59-62, Mar. 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-893187

RESUMO

SUMMARY: In order to evaluate the effect on sexual behavior and spermatic parameters of white New Zealand rabbits, at the onset of puberty, they were fed a standard diet supplemented with canola oil (obesogenic). Two groups were formed; one of which received daily 180 g of a standard commercial diet supplemented with 13 ml of canola oil during 12 weeks, while the second group (control) were fed daily 180 g of only the standard commercial diet. Starting at 20-weeks of age, seminal volume, en masse and individual sperm motility, and sperm concentration per mm3 were measured from each individual, as well as the behaviors olfactory exploration, chinning, and first and second attempt at mounting were assessed. No significant differences were found between groups in terms of seminal volume, and en masse and individual sperm motility count and percentage (P>0.05). In relation to sexual behavior, the group fed with supplemental canola oil showed reduced the response time to females in the sexual behaviors of chinning, olfactory exploration, and first and second attempt at mounting when compared to the control group (P<0.01). The data indicate that the addition of canola oil to a standard rabbit diet at the start of puberty and continued for 12 weeks increases the body mass index and reduces the response time in sexual behaviors such as olfactory exploration, chinning, and second attempt at mounting when interacting with female stimulus. Nevertheless, the seminal parameters did not show any modification. Other studies are needed to show the benefits or disadvantages of the addition of varying inclusion percentages of other vegetable oils, to evaluate their effects on reproductive efficiency in breeder male rabbits.


RESUMEN: Con el propósito de evaluar en conejos Nueva Zelanda blanca de granja el efecto de una dieta estándar suplementada con aceite de canola (obesogénica) sobre el comportamiento sexual y los parámetros espermáticos al inicio de la pubertad, se formaron dos grupos. Un grupo recibió una dieta consistente de 180 g al día de una dieta estándar comercial adicionada con 13 ml de aceite de canola durante 12 semanas y al otro grupo (testigo) se le suministró 180 g por día de la misma dieta estándar sin aceite de canola. A partir de las 20 semanas de edad en cada individuo se midió el volumen seminal, la motilidad espermática en masa e individual, la concentración espermática por mm3 y se evaluaron la conducta de: exploración olfatoria, frotamiento del mentón (Chinning), primer intento de monta y segundo intento de monta. En las variables volumen seminal, conteo y porcentaje de motilidad espermática en masa e individual no hubo diferencia significativa entre ambos grupos (P>0,05). Con respecto al comportamiento sexual, el grupo suplementado con aceite vegetal presentó menor tiempo de respuesta a la hembra estímulo en las conductas de frotamiento del mentón, exploración olfatoria y segundo intento de monta con respecto al grupo testigo (P<0,01). Los datos obtenidos indican que la adición de aceite de canola a la dieta estándar de conejos al inicio de la pubertad durante 12 semanas aumenta el índice de masa corporal de los individuos y disminuye el tiempo de respuesta en las conductas de exploración olfatoria, de frotamiento del mentón y de segundo intento de monta al interaccionar con la hembra estímulo. Sin embargo, no modificó los parámetros seminales evaluados. Es necesario realizar otros estudios con el fin de evaluar en conejos sementales el beneficio o desventaja sobre su eficiencia reproductiva al utilizar diferentes porcentajes de inclusión en la dieta de otros aceites vegetales.


Assuntos
Animais , Masculino , Óleo de Brassica napus/farmacologia , Coelhos , Sêmen/efeitos dos fármacos , Comportamento Sexual Animal/efeitos dos fármacos , Suplementos Nutricionais , Maturidade Sexual
4.
J Gastrointest Oncol ; 7(4): 632-7, 2016 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-27563455

RESUMO

BACKGROUND: Stromal tumors of the digestive tract are uncommon malignant diseases, are subclassified as leiomyosarcomas and Gastrointestinal Stromal Tumors (GIST) depending on the molecular expression of tyrosine kinase receptor KIT (CD117). GISTs represent 1% of malignant tumors affecting this anatomical site. Localized tumours diseases are reasonably well controlled by surgical resection and several criteria define the need for adjuvant therapy. In the case of metastatic disease a poor prognosis has been reported with systemic treatment based on chemotherapy. Recently, significant advances have been shown since tyrosine kinase inhibitors (TKIs) were introduced, with median overall survival close to 5 years. Unfortunately in Mexico, even though the therapy has been long used there are no published data of the experience in the treatment of these tumors. METHODS: We used an electronic data base to obtain clinical, radiological and histological data of patients diagnosed with GIST and treated in the oncological center of the Mexican Institute of Social Security, patients were subclassified by stage, symptoms at diagnosis as well as the initial and subsequent systemic treatment. Finally we made an analysis for progression free survival and overall survival identifying prognostic factors. RESULTS: We obtained information of 71 patients with metastatic, non-resectable or recurrent GIST, treated with a TKI, we observed a predominant relation for women (60.4%) with median age of 58 years. Stage at diagnosis was predominantly metastatic (46.5%), most frequently affected sites were lung, liver and retroperitoneum. Median progression free survival was 30.6 months and overall survival was 81.3 months. All patients were initially treated with imatinib at a dose of 400 mg per day. Treatment was well-tolerated in most cases. CONCLUSIONS: Metastatic GIST evaluated in our center shows a different affection in gender and age, and our population shows a different response to TKIs, compared to those reported in other series with superior overall survival. Poor prognosis is associated with lung affection. Biological studies will be started for the molecular evaluation of these tumors.

5.
Int. j. morphol ; 33(4): 1371-1376, Dec. 2015. ilus
Artigo em Inglês | LILACS | ID: lil-772324

RESUMO

Two groups were formed in order to evaluate the effect of an obesogenic diet on body and liver weight, body fat accumulation and the histometric characteristics of liver and testicles of New Zealand rabbits during the peripuberal period. One group received a standard farm diet and the other received a standard farm diet with canola oil added. During the study, weight of both groups was recorded. At the end of the study, the rabbits were euthanized and liver, testicles, and visceral, scapular and scrotal fat were weighed. Tissue samples were obtained from liver and testicles to carry out histometric analysis. Body weight, body mass index, liver and visceral fat weight were significantly different (P<0.01) in the obesogenic diet when compared to the control group. Notwithstanding, there were no significant differences between both groups in testicular weight, and scapular and scrotal fat (P>0.01). Histometry of the liver of the obesogenic diet group showed the greater total area and greater nucleus area of hepatocytes in the square lobe, right lobe and left lobe medial segment (P<0.01). Histometry of both testicles of individuals from the obesogenic diet group had a greater germinal epithelial thickness and intertubular intersticium (P<0.01). It is concluded that New Zealand male rabbits that received an obesogenic diet during 12 weeks had a higher liver and body weight, as well as, an important increased visceral fat. Furthermore, said diet caused histometric changes in liver and testicles. This set confirms that the domestic rabbit is a good model for the study of the body fat accumulation process associated to the consumption of an obesogenic diet and its effects on liver and testicles.


Con el propósito de evaluar el efecto de una dieta obesogénica sobre el peso corporal, hepático, acumulo de grasa corporal y las características histométricas del hígado y testículo de conejos Nueva Zelanda durante el período peripuberal, se formaron dos grupos; a uno se le proporcionó una dieta estándar de granja y el otro recibió una dieta estándar de granja adicionada con aceite de canola. Durante el estudio se registró el peso corporal de ambos grupos. Al final del estudio los conejos fueron eutanasiados y se pesó el hígado, testículos, la grasa visceral, escapular y escrotal. Se obtuvieron muestras de tejido hepático y testicular para realizar el análisis histométrico. El grupo con dieta obesogénica presentó diferencias significativas con respecto al grupo testigo, en las variables: peso corporal, índice de masa corporal, peso del hígado y de la grasa visceral (P<0,01). Por otra parte, no hubo diferencias significativas entre ambos grupos para las variables: peso de testículos, grasa escapular y escrotal (P>0,01). En la histometría del hígado el grupo con la dieta obesogénica presentó mayor área total y mayor área del núcleo de los hepatocitos en el lóbulo cuadrado, lóbulo derecho y lóbulo izquierdo segmento medial (P<0,01). En la histometría de ambos testículos del grupo con la dieta obesogénica se encontró mayor grosor del epitelio germinal y perímetro del intersticio intertubular (P<0,01). Se concluye que los conejos Nueva Zelanda machos que recibieron una dieta obesogénica durante 12 semanas presentaron mayor peso corporal, hepático y aumento de la grasa visceral. Además, presentaron cambios histométricos en hígado y testículo. Estos resultados en conjunto confirman que el conejo doméstico es un buen modelo para el estudio del proceso de acumulo de grasa corporal asociado al consumo de una dieta obesogénica y de sus efectos en el hígado y testículos.


Assuntos
Animais , Masculino , Coelhos , Peso Corporal/efeitos dos fármacos , Gorduras na Dieta/administração & dosagem , Fígado/efeitos dos fármacos , Testículo/efeitos dos fármacos , Tecido Adiposo , Índice de Massa Corporal , Obesidade/induzido quimicamente , Puberdade
7.
Parasit Vectors ; 8: 285, 2015 May 24.
Artigo em Inglês | MEDLINE | ID: mdl-26003809

RESUMO

BACKGROUND: The mite Psoroptes cuniculi is a common worldwide ectoparasite and the most frequently found in rabbit farms. It causes significant economic losses on commercial rabbit breeding associated with poor leather quality, reduced conception rates, weight loss, poor growth and death. Several strategies have been proposed for the treatment of mange caused by this mite, ranging from the use of acaricides, entomopathogenic fungi, essential oils and vaccines. However, therapy and control of both human scabies and animal mange are still based mainly on the use of drugs and chemicals such as ivermectin, which involves disadvantages including genotoxic and cytotoxic effects, resistance and environmental damage. Bacillus thuringiensis is a bacterium, innocuous for human being, domestic animals and plants that produces highly biodegradable proteins, and has been used worldwide for biological control. The aim of this work was to find an alternative treatment based on biological control for scabies caused by Psoroptes cuniculi, using protein extracts from strains of Bacillus thuringiensis. METHODS: P. cuniculi mites were obtained from naturally infected New Zealand rabbits, and different doses of protein from B. thuringiensis were added to the mites. We measured mortality and obtained the median lethal concentration and median lethal times. For histological analysis, the mites were fixed in 10% formalin, processed according to the paraffin embedded tissue technique. Sections were stained with hematoxylin-eosin to observe the general histological structure. RESULTS: We report here for the first time evidence about the in vitro acaricidal effect caused by the strain GP532 of B. thuringiensis on the mite Psoroptes cuniculi, with an LC50 of 1.3 mg/ml and a LT50 of 68 h. Histological alterations caused by B. thuringiensis on this mite, included the presence of dilated intercellular spaces in the basal membrane, membrane detachment of the peritrophic matrix and morphological alterations in columnar cells of the intestine. CONCLUSIONS: Since this mite is an obligate ectoparasite that affects rabbits, goats, horses, cows and sheep, B. thuringiensis protein extracts are proposed as a potential treatment for biological control of mange in farm animals.


Assuntos
Acaricidas/farmacologia , Bacillus thuringiensis/química , Proteínas de Bactérias/farmacologia , Infestações por Ácaros/veterinária , Controle Biológico de Vetores/métodos , Psoroptidae/efeitos dos fármacos , Coelhos/parasitologia , Acaricidas/isolamento & purificação , Animais , Proteínas de Bactérias/isolamento & purificação , Feminino , Masculino , Infestações por Ácaros/tratamento farmacológico
8.
Int. j. morphol ; 32(2): 732-737, jun. 2014. ilus
Artigo em Inglês | LILACS | ID: lil-714337

RESUMO

The objective of this study was to establish in New Zealand female rabbits, the effect of ordinal number of parturitions on some histological parameters on the day after weaning. Tissue fragments of uterus and vagina were obtained from females of first, second and third parturitions and were processed imbedding them in paraffin in order to do histological cuts. In eight microscope fields captured by a camera connected to an optic microscope, the lumen and glandular endometrial epithelium height, as well as the thickness of vaginal and myometrium epithelium thickness were measured. From the results that were obtained it was concluded that there are significant differences (p<0.05) in histological parameters evaluated in uterus and vagina between does of different parturitions, which indicates that the number of parturitions has an effect on histometric characteristics of genital tract organs in breeder female rabbits.


El objetivo del presente estudio fue determinar en conejas Nueva Zelanda el efecto del número ordinal de partos sobre algunos parámetros histológicos, en el día posterior al destete. A partir de hembras de primero, segundo y tercer parto se obtuvieron fragmentos de tejido uterino y vaginal los que se procesaron mediante la técnica de inclusión en parafina para efectuar cortes histológicos. En ocho campos microscópicos capturados con una cámara conectada a un microscopio óptico se midió la altura del epitelio luminal y glandular del endometrio; el grosor del epitelio de la vagina y del miometrio. A partir de los resultados obtenidos se concluye que existen diferencias significativas (p<0,05) en los parámetros histológicos evaluados en útero y vagina entre las conejas de distintos partos, lo que indica que el número de partos tiene un efecto sobre las características histométricas de los órganos del tracto genital de la coneja.


Assuntos
Animais , Feminino , Gravidez , Coelhos , Coelhos/anatomia & histologia , Útero/anatomia & histologia , Vagina/anatomia & histologia , Parto , Desmame
9.
Int. j. morphol ; 31(3): 932-936, set. 2013. ilus
Artigo em Inglês | LILACS | ID: lil-694981

RESUMO

Most of the neotropical bats reproduce in a seasonal fashion. The purpose of this study was to assess changes in testicular tissue by morphometry, in Tadarida brasiliensis bat, in a south urban zone of Mexico City during summer, autumn, and winter. Three sample collections (February, June, and September) from T. brasiliensis were carried out (n=6). Testicle fragments were obtained for histological studies. Diameter of the seminiferous tubules and interstitial space were measured, cellular populations were identified and counted. June samples showed smaller diameter of seminiferous tubules and larger interstitial space between tubules; also there were less number of germinal epithelium cells and spermatids were absent. Tissues from September and February showed a significant increase (p 0.05) in tubule diameter, germinal epithelium thickness, and number of germinal epithelium cells when compared to June samples. Only February samples showed presence of spermatids. Our results suggest the existence of seasonal variations in the reproductive activity of T. brasiliensis, under conditions in which the study was conducted.


La mayoría de los murciélagos neotropicales se reproducen de manera estacional. El objetivo de este estudio fue determinar los cambios morfométricos en el parénquima testicular del murciélago Tadarida brasiliensis que habita en un área urbana del sur de la Ciudad de México durante las estaciones de verano, otoño e invierno. Se obtuvieron fragmentos de tejido testicular para su estudio histológico. Se midió el diámetro de los túbulos seminíferos y el espacio interstisial y se identificaron y contaron distintos tipos celulares. En las muestras de junio se encontró un menor diámetro de los túbulos seminíferos y un mayor espacio intersticial entre los túbulos; también hubo un menor número de células del epitelio germinal y no hubo presencia de espermátides. En las muestras de tejido obtenidas en el mes de septiembre y febrero se observó un incremento significativo en el diámetro del túbulo, grosor del epitelio germinal y número de células del epitelio germinal cuando fueron comparadas con las muestras de junio (p 0.05). Solo en las muestras de febrero hubo presencia de espermátides. En conjunto, nuestros resultados sugieren la existencia de variaciones estacionales en la actividad reproductiva de T. brasiliensis, bajo las condiciones en que se realizó el estudio.


Assuntos
Animais , Quirópteros/anatomia & histologia , Testículo/anatomia & histologia , México , Estações do Ano , Área Urbana
10.
Int. j. morphol ; 31(3): 991-996, set. 2013. ilus
Artigo em Inglês | LILACS | ID: lil-694991

RESUMO

The purpose of this study was to analyze the macroscopic morphometry of the ovaries, uterus and vagina in rabbit does in the first, second and third parturition at 24 hours post-weaning in order to determine if there are differences between parturitions. Weaning of the litter was performed at 30 days post-partum and 24 hours later the does were euthanized. Right and left ovaries, uterine horns-cervix and vagina were removed, and the length of each one was measured. Significant differences were found in the average of the right ovary length between the first group with respect to second and third parturition group (P 0.05 and P 0.01). Also, left ovary length was different between the first and second with respect to third parturition group (P <0.001). Average total ovary length increased significantly as the number of parturitions increased in second and third parturition groups (P 0.01 and P0.001). The weight of the left and right ovaries was higher in does in their second and third parturition when compared to those in their first (P 0.5; P 0.01). The average total ovary weight increased significantly as the number of parturitions in second and third parturition groups (P0.001). The length of the left and right uterine horn-cervix of the rabbits after second and third parturition decreased when compared to that of females in their first parturition (P 0.05; P0.001). The average total uterine horns-cervix length decreased significantly as the number of parturitions in second and third parturition groups (P0.001). Furthermore, vagina length in females in their third parturition was greater than that of first and second parturition (P 0.01).The color of the vulva of the majority of females in their first parturition (3/4) was pale pink while. In contrast, the majority of females in their second parturition (3/4) had their vulva intensely red. All of the females (4/4) in the third parturition group had their vulva intensely red...


El propósito de este estudio fue analizar la morfometría macroscópica de los ovarios, útero y vagina en conejas de primero, segundo y tercer parto a las 24 horas post-destete con el propósito de determinar si existen diferencias entre los partos. El destete de la camada se realizó a los 30 días después del parto y 24 horas después fueron eutanasiadas. De cada animal se removieron los ovarios derecho e izquierdo, cuernos-cuello uterinos derecho e izquierdo y la vagina y se midió la longitud de cada uno de los órganos genitales. Se encontraron diferencias significativas en la longitud del ovario derecho entre el grupo de primer parto con respecto al de segundo y tercer parto (P 0,05 y P 0,01). Además, la longitud media de ovario izquierdo fue significativamente diferente entre el grupo de primero y segundo parto con respecto al de tercer parto (P <0,001). La longitud total de los ovarios aumentó con el número de partos y fue mayor en los grupos de segundo y tercer parto (P 0,01 y P 0,001). El peso de los ovarios derecho e izquierdo fue mayor en el grupo de segundo y tercer parto al compararlo con el de primero parto (P 0,5; P 0,01). Por otra parte, el peso total de los ovarios aumentó en los grupos de segundo y tercer parto (P 0,001). La longitud del cuerno-cuello uterino izquierdo y derecho de los grupos de segundo y tercer parto disminuyó al compararlo con el de las hembras de primer parto (P 0,05; P 0,001). La longitud total del cuerno-cuello uterino izquierdo y derecho disminuyó significativamente en las hembras de segundo y tercer parto (P 0,001). La longitud de la vagina de hembras de tercer parto fue mayor que la de primero y segundo parto (P 0,01). El color de la vulva de la mayoría de las hembras de primer parto (3/4) fue rosa pálido. En contraste, la mayoría de las hembras en su segundo parto (3/4) presentaron vulva de color rojo intenso. Todas las hembras del grupo de tercer parto (4/4) presentaron vulva de color rojo intenso...


Assuntos
Animais , Feminino , Coelhos , Coelhos/anatomia & histologia , Genitália Feminina/anatomia & histologia , Período Pós-Parto , Desmame
11.
PLoS One ; 8(6): e65083, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23762288

RESUMO

A decrease in fertility can have a negative economic impact, both locally and over a broader geographical scope, and this is especially the case with regard to the cattle industry. Therefore, much interest exists in evaluating proteins that might be able to increase the fertility of sperm. Heparin binding proteins (HBPs), specifically the fertility associated antigen (FAA) and the Type-2 tissue inhibitor of metalloproteinase (TIMP-2), act to favor the capacitation and acrosome reaction and perhaps even modulate the immune system's response toward the sperm. The objective of this research was to determine the effect on fertility of adding recombinant FAA (rFAA) and recombinant TIMP-2 (rTIMP-2) to bovine semen before cryopreservation for use in an artificial insemination (AI) program in a tropical environment. For this experiment, 100 crossbred (Bos taurus x Bos indicus) heifers were selected based on their estrus cycle, body condition score (BCS), of 4 to 6 on a scale of 1 to 9, and adequate anatomical conformation evaluated by pelvic and genital (normal) measurements. Heifers were synchronized using estradiol benzoate (EB), Celosil® (PGF2α) (Shering-Plough) and a controlled internal drug release (CIDR) device was inserted that contained progesterone. Inseminations were performed in two groups at random, 50 animals per group. The control group was inseminated with conventional semen. The treatment group was inseminated with semen containing rFAA (25 µg/mL) and rTIMP-2 (25 µg/mL). In the control group a 16% pregnancy rate was obtained versus a 40% pregnancy rate for the HBP treatment group, resulting in a significant difference (P = 0.0037). Given the results herein, one may conclude that the HBPs can increase fertility and could be an option for cattle in tropical conditions; however, one needs to consider the environment, nutrition, and the genetic interaction affecting the final result in whatever reproductive program that is implemented.


Assuntos
Fertilidade/fisiologia , Inseminação Artificial , Proteínas de Membrana/metabolismo , Taxa de Gravidez , Proteínas Recombinantes/metabolismo , Espermatozoides/metabolismo , Inibidor Tecidual de Metaloproteinase-2/metabolismo , Animais , Bovinos , Criopreservação , Sincronização do Estro , Feminino , Técnica Indireta de Fluorescência para Anticorpo , Humanos , Masculino , Proteínas de Membrana/administração & dosagem , Ovulação , Gravidez , Proteínas Recombinantes/administração & dosagem , Sêmen/metabolismo , Inibidor Tecidual de Metaloproteinase-2/administração & dosagem
12.
Vet. Méx ; 41(3): 211-218, jul.-sep. 2010. ilus, tab
Artigo em Espanhol | LILACS-Express | LILACS | ID: lil-632946

RESUMO

Morphometric characteristics were determined from female reproductive organs of New Zealand rabbits with different body weight in order to understand the anatomical changes of the various organs of the reproductive tract of this breed in the peripuberal period of transition. Four groups were formed according to a percentage of adult weight (AW), Group A with 2 ± 0.1 kg (40% of AW), Group B with 3 ± 0.1 kg (60% of AW), Group C with 3.5 ± 0.1 kg (70% of AW) and Group D with 4 ± 0.1 kg (80% of AW). The rabbits were euthanized according to the required weight for each group and ovaries, uterine horns, cervix and vagina were removed, in order to measure their length. Ovarian tissue fragments were obtained and processed by paraffin embedding for histological analysis using Masson's trichrome stain. Primordial, primary, secundary and tertiary follicles were counted in the ovarian cortex and medulla. Regarding the length of the left and right ovary there were differences between Group A with respect to B, C and D (P < 0.05). Regarding the length of the uterine horns there were differences between Group A with respect to groups B, C and D in both horns (P < 0.05). Regarding the length of the cervix there was difference between Group B and the others (P < 0.05) and the vagina showed difference between Group A and the other groups (P < 0.05). With respect to the variable number of primordial follicles there was difference between Group A and the other groups (P < 0.05). For primary and secondary follicles there were no differences between the four groups (P > 0.05). In tertiary follicles there were differences between Group A and the others (P < 0.05). Based on the results of this study it is concluded that in New Zealand rabbits the histological and morphometric changes of the reproductive tract associated with body weight, are more dramatic in the transition range from 40% to 60% (groups A and B) of AW, hence, it is a critical period in the maturation of the female reproductive system.


Se determinaron las características morfométricas de los órganos genitales de conejas de la raza Nueva Zelanda con distintos pesos corporales en el periodo de transición peripuberal. Se formaron cuatro grupos de acuerdo con un porcentaje del peso adulto (PA); Grupo A con 2 ± 0.1 kg (40% del PA); Grupo B con 3 ± 0.1 kg (60% del PA); Grupo C con 3.5 ± 0.1 kg (70% del PA) y Grupo D con 4 ± 0.1 kg (80% del PA). A las conejas se les sometió a eutanasia de acuerdo con el peso requerido para cada grupo. Se removieron ambos ovarios, cuernos uterinos, cérvix y vagina con el fin de medir su longitud. Se obtuvieron fragmentos de tejido ovárico que se procesaron por inclusión en parafina para efectuar cortes histológicos que se tiñeron con tricrómica de Masson. Se contaron los folículos primordiales, primarios, secundarios y terciarios presentes en la corteza y médula ovárica. Respecto de la longitud de los ovarios derecho e izquierdo, se encontraron diferencias entre el Grupo A y los grupos B, C y D (P < 0.05). En cuanto a la longitud de los cuernos uterinos, se encontraron diferencias entre el Grupo A y los grupos B, C y D, en ambos cuernos (P < 0.05). En relación con la longitud del cérvix, hubo diferencia entre el Grupo A y los demás grupos (P < 0.05); en la vagina se encontró diferencia entre el Grupo A y el resto de los grupos (P < 0.05). Acerca del número de folículos primordiales, hubo diferencia entre el Grupo A y los otros grupos (P < 0.05). Para los folículos primarios y secundarios no se encontraron diferencias entre los cuatro grupos (P > 0.05). En los folículos terciarios hubo diferencias entre el Grupo A y los demás grupos (P < 0.05). Con base en los resultados obtenidos se concluye que en la coneja Nueva Zelanda los cambios histológicos y morfométricos de los órganos genitales asociados con el peso corporal, son más drásticos en el rango de transición de 40% al 60% (grupos A y B) del PA. Se concluye que se trata de un periodo crítico en el proceso de maduración del aparato reproductor femenino.

13.
Anim Reprod Sci ; 120(1-4): 173-8, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20434280

RESUMO

Progesterone receptor (PR) plays an important role in mammals pregnancy which is characterized by greater progesterone plasma concentrations. We assessed PR protein distribution in the rabbit uterus by immunohistochemistry in two progestational conditions: pseudopregnancy (intact adult animals treated with hCG) and after application of a synthetic progestin, medroxyprogesterone acetate (MPA), to ovariectomized animals (OVX). PR immunoreactivity in uterine epithelium of pseudopregnant rabbits was increased in relation to non-pseudopregnant (NP) rabbits. Amounts were similar on Days 1, 3, and 5 of treatment, and was greater on Day 7 (P<0.001). In contrast, a significant diminution in PR immunoreactivity was observed in stroma cells from Days 1 to 7 (P<0.001). In OVX rabbits treated with MPA, an increase in PR immunoreactivity was observed in the uterine epithelium on Days 1 to 5 of treatment, reaching a maximum on Day 3 (P<0.001). In contrast, in stromal cells a diminution in PR immunoreactivity was observed when compared to the OVX group on Days 1, 3 and 7 of MPA treatment (P<0.001), and there was a slight increase on Day 5. Results suggest a differential time course and tissue specific immunoreactivity for PR in the uterus of the rabbit in two progestational conditions. The present study indicated synthetic progestins have different mechanisms of receptor regulation than those of natural hormones and it should be taken into account in reproductive applications.


Assuntos
Acetato de Medroxiprogesterona/farmacologia , Pseudogravidez/metabolismo , Receptores de Progesterona/metabolismo , Útero/efeitos dos fármacos , Útero/metabolismo , Animais , Gonadotropina Coriônica/farmacologia , Anticoncepcionais/farmacologia , Avaliação Pré-Clínica de Medicamentos , Feminino , Imuno-Histoquímica , Ovariectomia , Progesterona/sangue , Pseudogravidez/sangue , Pseudogravidez/patologia , Coelhos , Fatores de Tempo , Útero/patologia
14.
Vet. Méx ; 40(1): 9-16, ene.-mar. 2009. ilus
Artigo em Espanhol | LILACS-Express | LILACS | ID: lil-632898

RESUMO

Dog overpopulation is a major health problem in developing countries due to the existence of some zoonotic diseases in which dogs act as reservoirs, besides the aggressive events to humans. Distribution, behavior patterns and combined methodologies are needed aspects in the design of successful dog population control programs. Coumestrol is a phytoestrogen which induces alterations in the reproductive male system, when bind to alpha and beta estrogen receptors acting as an agonist or antagonist fashion. Both receptor types also exist in central nerve regions governing sexual behavior of those animals such as the preoptic area, ventro medial nucleous, the amygdala and the olfactory bulb. In this study, 300 μg/kg coumestrol was orally administered to male dogs, once a week for a 4 week period. Dogs were freed for 5 min in a 9 m² area having a recipient containing vaginal discharges from estrous dog females and other similar vessel containing sterile saline solution. Smelling latency time for each recipient, smelling frequency and territory marking in response to stimulus, was recorded. At the end of the test, semen was collected and evaluated. A significative difference (P < 0.005) in smelling latency time and smelling frequency to the vaginal discharge was found; sperm count decreased from 518.4 ± 215.4 × 10(6) to 57.6 ± 50.4 × 10(6) at week 4 and the abnormal sperm morphology increased from 14.7 ± 3.3% at 0 week to 60.0 ± 20%. In conclusion, 300 μg/kg coumestrol given orally to male dogs for 4 weeks induces alterations in the olfactory behavior along with an oligo and teratospermic effect.


La sobrepoblación canina es un problema importante de salud pública debido a la transmisión de enfermedades zoonóticas y las agresiones hacia el humano. En el diseño de programas para controlar la población canina se requiere del conocimiento de su distribución, comportamiento y metodologías combinadas para tener éxito. El coumestrol es un fitoestrógeno que induce alteraciones en el aparato reproductor de los machos al unirse a los receptores estrogénicos alfa y beta, en donde actúa de manera dosis-dependiente como agonista o antagonista. Estos receptores también existen en las estructuras del sistema nervioso que regulan el comportamiento sexual, como la región preóptica, núcleo ventromedial, la amígdala y el bulbo olfatorio. En este estudio se administró coumestrol (300 μg/kg) por vía oral a perros machos, una vez por semana durante cuatro semanas; los perros se colocaron durante cinco minutos en un área aislada de 9 m² en donde se colocó un frasco conteniendo secreciones vaginales obtenidas de perras en estro y otro con solución salina estéril. Se registró el tiempo de latencia de los perros para olfatear cada frasco, su frecuencia de respuesta y la frecuencia con que se presentó conducta de marcaje en respuesta al estímulo. Una vez concluido el experimento, se obtuvo semen y se evaluó. Se encontró diferencia significativa (P < 0.005) en el periodo de latencia y frecuencia con la que el macho se acercó a oler las secreciones vaginales y el conteo espermático disminuyó de 518.4 ± 215.4 × 10(6) al inicio del estudio a 57.6 ± 50.4 × 10(6) en la semana cuatro y el porcentaje de anormalidades espermáticas aumentó de 14.7 ± 3.3 puntos en la semana 0 a 60.0 ± 20.0% en la semana cuatro. Se concluye que el tratamiento de perros con coumestrol durante cuatro semanas ocasiona alteraciones en la conducta de exploración olfatoria y tiene un efecto oligospérmico y teratospérmico.

15.
Vet Res Commun ; 33(1): 87-95, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18712487

RESUMO

Dog overpopulation is considered a human health risk; they are the terrestrial vector of rabies and reservoirs for other human diseases. Surgical neutering and intratesticular injections have been used in male dogs. Physiological and morphological alterations in reproductive organs can be induced by phytoestrogens. Our goal was to evaluate the effect of oral coumestrol on dog ejaculates and testis histology. Two groups of 5 healthy adult dogs were used. One coumestrolcontaining biscuit was given once a week for a 4 week period to the experimental group. Ejaculates were obtained and evaluated. After treatment, testis were obtained and processed for histology. Compared to controls, treated dogs have reduced tubules (462 +/- 1.4 vs 336 +/- 2 micron(2)), spermatogenic epithelium (49.1 +/- 0.01 vs 13.3 +/- 0.01 micron(2)), and lumen opening (891 +/- 1.4 vs 530 +/- 26.9 micron). Ejaculates from treated animals have increased numbers of abnormal spermatozoa and reduced sperm concentration.


Assuntos
Anticoncepcionais Masculinos/farmacologia , Cumestrol/farmacologia , Fitoestrógenos/farmacologia , Testículo/efeitos dos fármacos , Animais , Anticoncepcionais Masculinos/administração & dosagem , Cumestrol/administração & dosagem , Cães , Masculino , Fitoestrógenos/administração & dosagem , Contagem de Espermatozoides/veterinária
16.
Neuro Endocrinol Lett ; 29(6): 977-80, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19112405

RESUMO

INTRODUCTION: Estrogens are well recognized as important hormones in male reproduction and act as ligands to alpha and beta estrogen receptors. Both estrogen receptors could interact with estrogen-mimicking compounds such as the fluorescent phytoestrogen coumestrol, which acts both in an agonist or antagonist fashion. OBJECTIVE: To investigate the presence of Coumestrol-Estrogen Receptor complexes by fluorescence in testis and epididymis, its effect in the ER expression by immunostain in the same tissues and the effect of this binding in the testis histological characteristics. DESIGN: Adult healthy and sexually active dogs were assigned to either the experimental or control group .Coumestrol impregnated dog biscuits were given to each animal from the experimental group once a week for a 4 week period. The control group received a biscuit with no Coumestrol, also once a week and for the same period. Testis morphology, ER immunodetection, and coumestrol-receptor binding were evaluated. SETTING: The experiment was done in the facilities of the Mexico City canine shelter. Animals were caged individually with food and water ad libitum and having at least two daily hours for exercise. RESULTS: Morphological alterations in testis after oral administration of coumestrol were detected. The main alterations include decreased germinal epithelium in tubule, and the loss of a continuous proliferation and differentiation gamete layer. Fluorescence signals in testis interstitial Leydig cells and epididymus indicating ER-coumestrol complexes were detected at the same points to those Immunohystochemically detected ER. CONCLUSIONS: Coumestrol administration induces testis alterations and coumestrol-ER complexes can be co-localized by binding-enhanced fluorescence and immunoprecipitation.


Assuntos
Cumestrol/farmacologia , Epididimo/efeitos dos fármacos , Fitoestrógenos/farmacologia , Receptores de Estrogênio/metabolismo , Testículo/efeitos dos fármacos , Administração Oral , Animais , Cumestrol/administração & dosagem , Cães , Epididimo/metabolismo , Masculino , Fitoestrógenos/administração & dosagem , Distribuição Aleatória , Receptores de Estrogênio/efeitos dos fármacos , Túbulos Seminíferos/efeitos dos fármacos , Testículo/metabolismo
17.
Comp Biochem Physiol A Mol Integr Physiol ; 147(3): 685-690, 2007 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-16931081

RESUMO

We characterized the expression pattern of progesterone receptor (PR) in two regions of the oviduct (ampullae and isthmus), and the uterus (epithelium and stroma) of the rabbit (Oryctolagus cuniculus) during early pregnancy (1-4 days) by RT-PCR and immunohistochemistry. We observed a significant increase in the expression of PR at mRNA level in the uterus on days 1 and 2 of pregnancy, followed by a decrease on days 3 and 4. These changes were also observed at protein level in the uterine epithelium. Interestingly, PR immunoreactivity decreased in stromal cells in all days of pregnancy as compared with non-pregnant rabbits (NG). In the isthmus PR mRNA expression significantly increased on day 2 of pregnancy and diminished on days 3 and 4, whereas no significant changes were observed in the ampullae. In epithelial and stromal cells of the isthmus, PR immunostaining was reduced through pregnancy as compared with NG group. In contrast, a reduction in PR immunostaining was observed on days 1-3 with an increase on day 4 in epithelial and stromal cells of the ampullae. The overall results suggest that PR exhibit a differential expression pattern in the oviduct and the uterus during early pregnancy of the rabbit, and that these differences are related to different functions of PR in the reproductive tract during early pregnancy.


Assuntos
Tubas Uterinas/metabolismo , Regulação da Expressão Gênica , Receptores de Progesterona/genética , Receptores de Progesterona/metabolismo , Útero/metabolismo , Animais , Tubas Uterinas/citologia , Feminino , Imuno-Histoquímica , Gravidez , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Coelhos , Útero/citologia
18.
Theriogenology ; 62(3-4): 532-43, 2004 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-15226009

RESUMO

Goat ovarian follicles produce estrone and estradiol from androgens. The synthesis of C18 estrogens from C19 androgens requires cytochrome P450 aromatase, but little information about this key enzyme is available in the goat. We report here for the first time the cDNA sequence of the goat ovarian aromatase, the activity of the enzyme in a cell system, and its expression in the term goat placenta. A cDNA library from goat ovarian poly(A)+ RNA was constructed. Human aromatase cDNA was selected as probe to screen the library; several clones were isolated, but none was complete. The longest clone was 3.1 kb long, but it lacked the sequence coding for a few amino acids in the NH(2)-terminal. To obtain the missing sequence, we performed reverse amplification of the cDNA end (RACE). Sequence analysis indicated that goat aromatase possessed a very long 3'-untranslated region ( approximately 1790 bp), and a polyadenylation signal (AATAAA) located at position 3320 downstream from the ATG start codon. The coding region of goat cDNA was inserted in an expression vector and transfected into HEK-293 cells that were cultured in presence of [14C]-androstenedione, steroids extracted and further separated by TLC. The transfected cells efficiently transformed [14C]-androstenedione into estrone. This activity was inhibited by 4-hydroxyandrostenedione. We also investigated the presence of mRNA for P450 aromatase in the goat placenta, using reverse transcription-polymerase chain reaction (RT-PCR) and primers derived from the cDNA ovarian sequence and confirmed the expression of the mRNA in term placenta.


Assuntos
Aromatase/genética , DNA Complementar/química , DNA Complementar/isolamento & purificação , Cabras/metabolismo , Ovário/enzimologia , Placenta/enzimologia , Sequência de Aminoácidos , Animais , Aromatase/química , Aromatase/metabolismo , Sequência de Bases , Clonagem Molecular , Feminino , Humanos , Dados de Sequência Molecular , RNA Mensageiro/análise , Homologia de Sequência
19.
Vet. Méx ; 32(1): 7-12, ene.-mar. 2001. ilus, tab, CD-ROM
Artigo em Espanhol | LILACS | ID: lil-303161

RESUMO

El propósito del presente estudio fue evaluar el efecto del zeranol sobre la estructura histológica del útero de chinchillas implantadas con este compuesto por vía subcutánea a dosis de 12 mg/animal. Se utilizaron 30 chinchillas con las que se formaron dos grupos, uno de hembras vírgenes de 12 a 15 meses de edad y otro de hembras adultas de 24 a 30 meses de edad, con partos previos. Cada uno de estos grupos tuvo su respectivo testigo. Los animales fueron sacrificados y se obtuvieron fragmentos de útero para efectuar su evaluación histológica. En los animales implantados, de ambas edades, los hallazgos más relevantes fueron aumento de dos a tres veces las dimensiones del útero (determinado en campos microscópicos), congestión vascular en la lámina propia y en el miometrio. Asimismo, se observaron células epiteliales en mitosis o apoptosis en los animales implantados de 12 a 15 meses de edad, mientras que en las hembras con edad de 24 a 30 meses se identificaron de cuatro a cinco veces más células mitóticas o apoptóticas, respectivamente, en las hembras implantadas en relación con las no implantadas; se cuantificó un mayor número de glándulas endometriales en la mayoría de los animales implantados de ambas edades. En el endometrio de los animales no implantados no se observó congestión vascular y sólo en 20 por ciento de los animales de 24 a 30 meses de edad se observó mitosis o apoptosis. Estos resultados demuestran que el zeranol a dosis de 12 mg/ animal induce en el útero de las chinchillas jóvenes y adultas, cambios en los ámbitos vascular y tisular, característicos de un estado estrogénico predominante.


Assuntos
Animais , Zeranol , Chinchila , Útero , Endométrio
20.
Vet. Méx ; 30(4): 317-21, oct.-dic. 1999. tab, ilus
Artigo em Espanhol | LILACS | ID: lil-266736

RESUMO

Los mastocitos (MC) son células del tejido conjuntivo que participan activamente en los mecanismos de comunicación paracrina mediante la liberación de diversos mediadores químicos contenidos en sus gránulos. El presente estudio se efectuó con el propósito de evaluar la distribución de los MC en la glándula mamaria de la perra (Canis familiaris), en periodos activos e inactivo. Las muestras de tejido mamario se obtuvieron a partir de perras adultas. Dichas muestras se procesaron siguiendo el método de inclusión en parafina y los cortes histológicos obtenidos fueron teñidos con azul de tolouidina para posteriormente efectuar la cuantificación de MC por mm², considerando para este fin el estroma interalveolar y el estroma interlobulillar del tejido mamario. Durante el periodo activo de la glándula mamaria, la población de MC presentes en el estroma disminuye significativamente respecto del periodo inactivo (P< 0.05). Estos resultados en conjunto sugieren que los MC presentes en el estroma de la glándula mamaria de perra, participan activamente en los cambios proliferativos que experimentan el tejido conjuntivo laxo areolar, dependiendo del estadio funcional de la glándula


Assuntos
Animais , Feminino , Cães/anatomia & histologia , Glândulas Mamárias Animais/citologia , Mastócitos/citologia , Células Estromais , Cães/fisiologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...