RESUMO
Nitric oxide (NO) has a profound role in the generation, differentiation, survival, and physiology of neurons. We have created a novel transgenic model to study the action of NO in the adult brain, in which the neuronal isoform of NO synthase (nNOS) is expressed under control of the promoter of the calcium-calmodulin multifunctional kinase IIalpha (CaMKIIalpha) gene. We show that the transgenic nNOS RNA and protein are expressed in the cortex, the hippocampus and the striatum of the transgenic mice. We also show that expression of several genes involved in the protection of neurons from oxidative stress and cell death is not affected in neurons of the transgenic mice. Furthermore, generation of new cells is depressed in the neurogenic brain areas in transgenics. In addition, we analyze gene expression in the hippocampus of the transgenic animals using microarray RNA profiling and Q-PCR. Our experiments describe specific changes in cell division and gene activity in the CaMKII-nNOS transgenic model and demonstrate its utility for studying the action of NO in the adult brain.
Assuntos
Regulação Enzimológica da Expressão Gênica , Sistema Nervoso/metabolismo , Óxido Nítrico Sintase Tipo I/biossíntese , Óxido Nítrico Sintase Tipo I/genética , Óxido Nítrico/fisiologia , Animais , Apoptose/genética , Apoptose/fisiologia , Proteína Quinase Tipo 2 Dependente de Cálcio-Calmodulina , Proteínas Quinases Dependentes de Cálcio-Calmodulina/genética , Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Divisão Celular/genética , Córtex Cerebral/metabolismo , Corpo Estriado/metabolismo , Perfilação da Expressão Gênica , Hipocampo/metabolismo , Camundongos , Camundongos Transgênicos , Microscopia Confocal , Neurônios/fisiologia , Análise de Sequência com Séries de Oligonucleotídeos , Estresse Oxidativo/genética , Reação em Cadeia da Polimerase , Regiões Promotoras Genéticas , RNA/biossíntese , RNA/genética , TransgenesRESUMO
The New Zealand environment is low in selenium and iodine, and is therefore ideally suited for the study of these anionic trace elements. The aim of this study was to determine urinary excretion of selenium and iodine during pregnancy and postpartum as part of an investigation of the influence of pregnancy and lactation on selenium metabolism in women of low selenium status. In a double-blind placebo-controlled study, 35 women in the earliest stages of pregnancy and 17 non-pregnant women were recruited in Dunedin, New Zealand. Eighteen pregnant women received 50 microg selenium as L-selenomethionine, while the others received a placebo daily during pregnancy and 12 months postpartum. The non-pregnant women received the supplement, serving as a positive control. Blood samples and twenty-four hour urine samples were collected monthly during pregnancy and at 3, 6, and 12 months postpartum for analysis of selenium and iodine. Selenium content in plasma and urinary excretion of selenium fell during pregnancy; however, total excretion of selenium was greater during pregnancy than postpartum. Urinary iodine excretion was much lower than reported previously in New Zealand. Due to large intra- and inter-subject variability, no trends in iodide excretion were observed. Factors which influence urinary excretion of selenium include dietary intake, but more closely, plasma concentrations of selenium (which is probably related to total selenium pool), creatinine excretion and therefore lean body mass, and glomerular filtration rate. The exact mechanism and sequence of events remains unclear and future studies incorporating new speciation techniques are necessary.
Assuntos
Iodo/urina , Lactação/urina , Gravidez/urina , Selênio/urina , Creatina/metabolismo , Creatina/urina , Feminino , Humanos , Iodo/metabolismo , Rim/metabolismo , Taxa de Depuração Metabólica/fisiologia , Valores de Referência , Selênio/sangue , Selênio/farmacocinética , Oligoelementos/metabolismo , Oligoelementos/urinaRESUMO
Mutations and deletions in mitochondrial DNA (mtDNA) lead to a number of human diseases characterized by neuromuscular degeneration. Accumulation of truncated mtDNA molecules (delta-mtDNA) lacking a specific 4977-bp fragment, the common deletion, leads to three related mtDNA diseases: Pearson's syndrome; Kearns-Sayre syndrome; and chronic progressive external ophthalmoplegia (CPEO). In addition, the proportion of delta-mtDNA present increases with age in a range of tissues. Consequently, there is considerable interest in the effects of the accumulation of delta-mtDNA on cell function. The 4977-bp deletion affects genes encoding 7 polypeptide components of the mitochondrial respiratory chain, and 5 of the 22 tRNAs necessary for mitochondrial protein synthesis. To determine how the accumulation of delta-mtDNA affects oxidative phosphorylation we constructed a series of cybrids by fusing a human osteosarcoma cell line depleted of mtDNA (rho0) with enucleated skin fibroblasts from a CPEO patient. The ensuing cybrids contained 0-86% delta-mtDNA and all had volumes, protein contents, plasma-membrane potentials and mitochondrial contents similar to those of the parental cell line. The bioenergetic consequences of accumulating delta-mtDNA were assessed by measuring the mitochondrial membrane potential, rate of ATP synthesis and ATP/ADP ratio. In cybrids containing less than 50-55% delta-mtDNA, these bioenergetic functions were equivalent to those of cybrids with intact mtDNA. However, once the proportion of delta-mtDNA exceeded this threshold, the mitochondrial membrane potential, rate of ATP synthesis, and cellular ATP/ADP ratio decreased. These bioenergetic deficits will contribute to the cellular pathology associated with the accumulation of delta-mtDNA in the target tissues of patients with mtDNA diseases.
Assuntos
DNA Mitocondrial/genética , Deleção de Sequência , Difosfato de Adenosina/metabolismo , Trifosfato de Adenosina/biossíntese , Trifosfato de Adenosina/metabolismo , DNA Mitocondrial/metabolismo , Metabolismo Energético , Humanos , Membranas Intracelulares/metabolismo , Membranas Intracelulares/fisiologia , Cinética , Potenciais da Membrana , Microscopia Eletrônica , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Células Tumorais CultivadasRESUMO
1. Peroxynitrite is a short-lived and damaging oxidant that forms rapidly from the reaction of superoxide with nitric oxide. 2. In 1990, Joseph Beckman proposed that peroxynitrite contributed significantly to pathological oxidative stress in living tissues, and subsequent evidence strongly supports this proposal. 3. In this review, we outline the properties of peroxynitrite and discuss how it can affect biological systems and contribute to human pathologies.
Assuntos
Nitratos/fisiologia , Oxidantes/fisiologia , Animais , Humanos , Nitratos/metabolismo , Óxido Nítrico/metabolismo , Estresse Oxidativo/fisiologia , Espécies Reativas de Oxigênio/metabolismo , Superóxidos/metabolismoRESUMO
The aim of this study was to establish methodology for a survey of the iodine and selenium status of New Zealand residents, more specifically to investigate the correlation between fasting or random casual urine samples and 24 hour urines for iodine and selenium excretion. Sixty-two (31 M, 31 F) adults collected casual, fasting and 24 hour urine samples for analysis of iodide, selenium and creatinine. Plasma and serum samples were collected for analysis of selenium and glutathione peroxidase activity. Results indicated that fasting urine samples, but not casual urines, may give a reasonable estimate of urinary output of iodine and selenium on a population basis, but that 24 hour urines are necessary for diagnosis of iodine deficiency in an individual and for research purposes. The results for iodine also give no support for expressing iodine as the iodide-creatinine ratio, although there was some indication that the selenium-creatinine ratio might be useful. Significant correlations between total daily excretion of selenium and iodine and also for urinary concentrations of the two trace elements in fasting and in 24 hour urine specimens may reflect a relationship of selenium and iodine to body size which may have implications for dietary requirements of these trace elements. Alternatively the correlations may reflect a relationship between dietary intake of the two trace elements in a country in which food concentrations are low, and this needs further investigation.
Assuntos
Iodo/urina , Selênio/urina , Adolescente , Adulto , Estudos de Avaliação como Assunto , Jejum/urina , Feminino , Glutationa Peroxidase/sangue , Humanos , Iodetos/urina , Masculino , Pessoa de Meia-Idade , Nova Zelândia , Selênio/sangueRESUMO
Peroxynitrite is a biologically important oxidant that damages mitochondria in a number of ways. We investigated the interaction of peroxynitrite with the mitochondrial glutathione pool by measuring the formation of oxidised glutathione and glutathione-protein mixed disulfides in mitochondria exposed to either peroxynitrite or tert-butylhydroperoxide. In contrast to tert-butylhydroperoxide, peroxynitrite converts 40-50% of mitochondrial glutathione to products other than disulfides, primarily higher oxidation states of sulfur. These data show that peroxynitrite interacts with mitochondria quite differently from oxidants commonly used in studying mitochondrial oxidative stress. Peroxynitrite also induces a permeability transition in the mitochondrial inner membrane, and here we show that this permeability transition is prevented by the NAD(P)H-linked substrates glutamate and malate and by the thiol reagent dithiothreitol. Glutamate and malate prevented complete oxidation of the NAD(P)H pool by peroxynitrite or tert-butylhydroperoxide but did not prevent oxidation of the mitochondrial glutathione pool or the formation of glutathione-protein mixed disulfides. This study is consistent with regulation of the permeability transition by critical protein thiol groups, whose redox state responds to that of the mitochondrial NAD(P)H pool, but which do not equilibrate directly with the mitochondrial glutathione pool.
Assuntos
Glutationa/metabolismo , Fígado/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Niacinamida/metabolismo , Nitratos/farmacologia , Animais , Feminino , Permeabilidade/efeitos dos fármacos , Ratos , Ratos WistarRESUMO
The mitochondrial respiratory chain continually produces superoxide leading to high levels of mitochondrial oxidative stress. This oxidative damage has been attributed to the formation of hydroxyl radicals and hydrogen peroxide from superoxide. Alternatively, mitochondrial superoxide may react with nitric oxide forming the potent oxidant peroxynitrite, thus damaging mitochondrial protein, lipid and DNA. To test this hypothesis we induced mitochondrial superoxide formation in the presence of nitric oxide. Here we demonstrate that mitochondrial superoxide reacts with nitric oxide to form peroxynitrite, suggesting that mitochondria may be a significant intracellular source of peroxynitrite.
Assuntos
Mitocôndrias Cardíacas/metabolismo , Nitratos/metabolismo , Óxido Nítrico/metabolismo , Superóxidos/metabolismo , Animais , Antimicina A/análogos & derivados , Antimicina A/farmacologia , Bovinos , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Dano ao DNA , DNA Mitocondrial/metabolismo , Mitocôndrias Cardíacas/efeitos dos fármacos , Estresse Oxidativo/fisiologiaRESUMO
The effect of the parkinsonian neurotoxin, 1-methyl-4-phenylpyridinium (MPP+) together with nitric oxide donors on mitochondrial calcium homeostasis and membrane potential was investigated. Simultaneous exposure of calcium-loaded mitochondria to MPP+ and nitric oxide donors led to Cyclosporin A-sensitive mitochondrial calcium efflux and depolarisation. When MPP+ was replaced with the respiratory inhibitor rotenone, mitochondrial calcium efflux and depolarisation also occurred. As both MPP+ and rotenone induce mitochondrial superoxide formation, the possibility that calcium efflux and depolarisation were due to peroxynitrite formation from reaction of superoxide with nitric oxide was investigated. It was shown that simultaneous exposure of mitochondrial membranes to nitric oxide donors and rotenone led to peroxynitrite formation. The possible roles of nitric oxide, peroxynitrite, mitochondrial depolarisation, and calcium efflux in MPP+ toxicity are discussed.
Assuntos
1-Metil-4-fenilpiridínio/toxicidade , Cálcio/metabolismo , Ciclosporina/farmacologia , Dopaminérgicos/toxicidade , Mitocôndrias Hepáticas/efeitos dos fármacos , Óxido Nítrico/toxicidade , Animais , Bovinos , Sinergismo Farmacológico , Feminino , Membranas Intracelulares/efeitos dos fármacos , Potenciais da Membrana , Mitocôndrias Cardíacas/efeitos dos fármacos , Mitocôndrias Hepáticas/metabolismo , Nitratos/metabolismo , Ratos , Ratos Wistar , Superóxidos/metabolismoRESUMO
Nitric oxide reacts rapidly with superoxide to form the potent oxidant peroxynitrite. Mitochondria in vivo produce superoxide and in pathological situations the amount of superoxide produced increases; therefore, in the presence of nitric oxide, mitochondria will be a major site of peroxynitrite formation. Oxidative stress induces cyclosporin-A-sensitive mitochondrial calcium efflux and depolarisation which may contribute to tissue damage in pathological situations. To determine whether peroxynitrite could induce calcium efflux and depolarisation we exposed mitochondria to both nitric oxide and superoxide simultaneously, thus subjecting the mitochondria to a continual flux of peroxynitrite similar to that found in pathological situations. Our results show that: (a) exposure of mitochondria to nitric oxide plus superoxide induces cyclosporin-A-sensitive mitochondrial calcium efflux and depolarisation; (b) neither nitric oxide nor superoxide on their own induce calcium efflux or depolarisation under these conditions; (c) calcium efflux and depolarisation occur when peroxynitrite production (from nitric oxide and superoxide) exceeds about 0.99 +/- 0.03 nmol peroxynitrite.min-1 mg mitochondrial protein-1. This rate of production of peroxynitrite is similar in magnitude to superoxide formation by mitochondria in pathological situations, suggesting that mitochondria can produce sufficient peroxynitrite in vivo to cause mitochondrial calcium efflux and depolarisation. Our experiments suggest a plausible model for tissue damage when mitochondrial superoxide production increases, for example in ischaemia-reperfusion injury or following exposure to neurotoxins. In the presence of nitric oxide the mitochondrial superoxide production will lead to peroxynitrite formation which induces cyclosporin-A-sensitive mitochondrial calcium efflux and depolarisation. This disruption to mitochondrial function may in turn contribute to cell damage and death.
Assuntos
Cálcio/metabolismo , Ciclosporina/farmacologia , Mitocôndrias Hepáticas/efeitos dos fármacos , Nitratos/metabolismo , Óxido Nítrico/biossíntese , Superóxidos/metabolismo , Animais , Transporte Biológico , Feminino , Membranas Intracelulares/fisiologia , Potenciais da Membrana , Mitocôndrias Hepáticas/metabolismo , Ratos , Ratos WistarRESUMO
Superoxide reacts with nitric oxide to form peroxynitrite, a potent oxidising agent which may contribute to tissue damage in pathological situations such as inflammation and ischaemia/reperfusion. One mechanism by which oxidative stress damages tissues is the induction of a specific Cyclosporin A-sensitive mitochondrial calcium efflux pathway. Here we show that peroxynitrite induces calcium efflux from mammalian mitochondria and that this efflux is blocked by Cyclosporin A. These data suggest that disruption of mitochondrial calcium efflux may contribute to tissue damage when superoxide and nitric oxide are present together in vivo.