RESUMO
ABSTRACT: At our medical center, HIV nucleic acid tests are recommended when the HIV antigen-antibody screening immunoassay and antibody differentiation tests are discordant, but not done reflexively. A retrospective chart review found that 35% of discordant test results did not have HIV nucleic acid test completed as recommended.
Assuntos
Algoritmos , Infecções por HIV , Técnicas de Amplificação de Ácido Nucleico , Humanos , Infecções por HIV/diagnóstico , Estudos Retrospectivos , Masculino , Feminino , Adulto , Teste de HIV , RNA Viral , Programas de Rastreamento/métodos , Pessoa de Meia-Idade , HIV-1/isolamento & purificação , HIV-1/imunologia , Anticorpos Anti-HIV/sangue , Imunoensaio/métodosRESUMO
Our experiments aim to determine if decreasing the amount of phosphatidylcholine (PC) relative to phosphatidylethanolamine (PE) at the lipid droplet surface changes the localization of specific lipid droplet proteins. We manipulate lipid droplet phospholipids in both a cultured mouse hepatocyte (AML12) cell line and on synthetic lipid droplets. Decreasing the PC:PE ratio increases perilipin 2, decreases DGAT2, and does not change rab18 or lanosterol synthase levels on lipid droplets. These differences may be explained by the distinct structural motifs that mediate the protein-lipid droplet interactions.