[The list of the occupational disease and medical surveillance: the function of preventive medicine doctor]. / L'elenco delle nuove malattie professionali e sorveglianza sanitaria: il ruolo del medico competente.

The recent revision of tables of occupational diseases according to the article 3 and 211 of TU 1124/65 is helpful to working physician. This revision established the necessity of explain the link between the causative agent and disease and inserted the disease caused by biomechanics overload of upper limbs. The previous absence of these disease was in contrast to the duty of declaration according to the article 139 of TU 1124/65. The new decree allowed to define better the link between the causative agent and the target organ of neoplastic occupational disease. Even if the decree set in order the duties of working physician a simplification is needed. In short about health supervision, it's necessary to clarify the link between the duty of health supervision and working activities that can cause occupational disease inserted in the tables. For this reason the intervention of the new Consultative Commission, according to article 6 of decree 81/2008, is desirable.

Aislamiento y caracterización de antígenos principales en Anisakis simplex / Isolation and characterization of major antigens from Anisakis simplex

La anisakiasis o anisakidosis es una enfermedad causada por la larva de Anisakis simplex tras la ingesta de pescado crudo o poco cocinado. Se han descrito cuadros gastrointestinales, gastroalérgicos y un tercer grupo que correspondería con la llamada hipersensibilidad de Anisakis. Caracterizar los antígenos implicados en esta reacción alérgica fue la meta del estudio. Métodos: Tras la obtención de las larvas del parásito se realizó un marcaje metabólico con Leu-C14 y posterior homogeneizado de éstas y de las restantes larvas sin marcar. Se identificaron las distintas proteínas presentes en el parásito y en el huésped por PAGE-SDS y posterior autorradiografía, diferenciando así las proteínas que sintetiza el nematodo. Se fraccionó un extracto de Anisakis por filtración en gel (Sephacryl S-200), y se ensayaron las fracciones obtenidas por inmunodetección. Resultados: Se aisló una proteína de 22 KDa que estaba presente en el grupo de aquellas que sintetizaba el parásito. La fracción que contenía esta proteína fue reconocida en todos los casos en los sueros de pacientes hipersensibilizados a Anisakis simplex. Posteriormente se analizó su punto isoeléctrico, presentando un pl de aproximadamente 5.5. Conclusiones: Se ha logrado caracterizar una proteína de 22kDa y pl 5.5 que podría estar implicada en las reacciones de hipersensibilidad de algunos pacientes frente a Anisakis simplex. (AU)

Maturation of maritime pine (Pinus pinaster Ait.) seedlings after exposure to a period of continuous light.

Nine half-sib families of maritime pine (Pinus pinaster Ait.) of known adult performance were grown in continuous light at either 25 degrees C or 25/20 degrees C for 18 weeks. They were then exposed to a dormancy induction period followed by a dormancy release period and then grown for a further 9 weeks in a 16-h photoperiod at a day/night temperature of 25/20 degrees C. Seedlings exhibited great diversity in morphology at the end of the first growth period. The number of morphogenetic cycles varied between one and three and the form of the apical meristem ranged from a typical rosette to an adult-like bud. The type of seedling obtained at the end of the first growth period strongly influenced later growth, independently of the temperature regime. Maturity was proportional to the number of morphogenetic cycles achieved during the first growth period and was characterized by short growth duration, small primary needles and a high degree of fixed growth. The state of the apical meristem that underwent the dormancy period had less influence on the rate of maturation than the number of morphogenetic cycles. The time course of maturation was endogenously controlled and varied among traits. Conspicuous morphological differences were not associated with changes in the relationship between growth components at the phenotypic level. However, there seemed to be a shift in the genetic correlations between growth components after first budset.