Enhancing boreal forest resilience: A four-year impact of biochar on soil quality and fungal communities.

Boreal forests commonly suffer from nutrient deficiency due to restricted biological activity and decomposition. Biochar has been used as a promising strategy to improve soil quality, yet its impacts on forest soil microbes, particularly in cold environment, remains poorly understood. In this study, we investigated the effects of biochar, produced at different pyrolysis temperatures (500 °C and 650 °C) and applied at different amounts (0.5 kg·m-2 and 1.0 kg·m-2), on soil property, soil enzyme activity, and fungal community dynamics in a boreal forest over a span of two to four years. Our results showed that, four-year post-application of biochar produced at 650 °C and applied at 1.0 kg·m-2, significantly increased the relative abundance of Mortierellomycota and enhanced fungal species richness, α-diversity and evenness compared to the control (CK) (P < 0.05). Notably, the abundance of Phialocephala fortinii increased with the application of biochar produced at 500 °C and applied at 0.5 kg·m-2, exhibiting a positively correlation with the carbon cycling-related enzyme ß-cellobiosidase. Functionally, distinct fungal gene structures were formed between different biochar pyrolysis temperatures, and between application amounts in four-year post-biochar application (P < 0.05). Additionally, correlation analyses revealed the significance of the duration post-biochar application on the soil properties, soil extracellular enzymes, soil fungal dominant phyla, fungal community and gene structures (P < 0.01). The interaction between biochar pyrolysis temperature and application amount significantly influenced fungal α-diversity (P < 0.01). Overall, these findings provide theoretical insights and practical application for biochar as soil amendment in boreal forest ecosystems.

The impact of biochar on wood-inhabiting bacterial community and its function in a boreal pine forest.

Biochar is considered to be a possible means of carbon sequestration to alleviate climate change. However, the dynamics of the microbial community during wood decomposition after biochar application remain poorly understood. In this study, the wood-inhabiting bacterial community composition and its potential functions during a two-year decomposition period after the addition of different amounts of biochar (0.5 kg m-2 and 1.0 kg m-2), and at different biochar pyrolysis temperatures (500 °C and 650 °C), in a boreal Scots pine forest, were analyzed using Illumina NovaSeq sequencing combined with Functional Annotation of Prokaryotic Taxa (FAPROTAX). The results showed that the wood decomposition rates increased after biochar addition to the soil surface in the second year. Treatment with biochar produced at high temperatures increased the diversity of wood-inhabiting bacteria more than that produced at low temperatures (P < 0.05). The wood-inhabiting bacterial diversity and species richness decreased with decomposition time. The biochar treatments changed the wood-inhabiting bacterial community structure during the decomposition period. The pyrolysis temperature and the amount of applied biochar had no effect on the bacterial community structure but shifted the abundance of certain bacterial taxa. Similarly, biochar application shifted the wood-inhabiting bacterial community function in the first year, but not in the second year. The wood-inhabiting bacterial community and function were affected by soil pH, soil water content, and soil total nitrogen. The results provide useful information on biochar application for future forest management practices. Long-term monitoring is needed to better understand the effects of biochar application on nutrient cycling in boreal forests.

The role of terrestrial productivity and hydrology in regulating aquatic dissolved organic carbon concentrations in boreal catchments.

The past decades have witnessed an increase in dissolved organic carbon (DOC) concentrations in the catchments of the Northern Hemisphere. Increasing terrestrial productivity and changing hydrology may be reasons for the increases in DOC concentration. The aim of this study is to investigate the impacts of increased terrestrial productivity and changed hydrology following climate change on DOC concentrations. We tested and quantified the effects of gross primary production (GPP), ecosystem respiration (RE) and discharge on DOC concentrations in boreal catchments over 3 years. As catchment characteristics can regulate the extent of rising DOC concentrations caused by the regional or global environmental changes, we selected four catchments with different sizes (small, medium and large) and landscapes (forest, mire and forest-mire mixed). We applied multiple models: Wavelet coherence analysis detected the delay-effects of terrestrial productivity and discharge on aquatic DOC variations of boreal catchments; thereafter, the distributed-lag linear models quantified the contributions of each factor on DOC variations. Our results showed that the combined impacts of terrestrial productivity and discharge explained 62% of aquatic DOC variations on average across all sites, whereas discharge, gross primary production (GPP) and RE accounted for 26%, 22% and 3%, respectively. The impact of GPP and discharge on DOC changes was directly related to catchment size: GPP dominated DOC fluctuations in small catchments (<1 km2 ), whereas discharge controlled DOC variations in big catchments (>1 km2 ). The direction of the relation between GPP and discharge on DOC varied. Increasing RE always made a positive contribution to DOC concentration. This study reveals that climate change-induced terrestrial greening and shifting hydrology change the DOC export from terrestrial to aquatic ecosystems. The work improves our mechanistic understanding of surface water DOC regulation in boreal catchments and confirms the importance of DOC fluxes in regulating ecosystem C budgets.

Water quality and the biodegradability of dissolved organic carbon in drained boreal peatland under different forest harvesting intensities.

Boreal peatlands are major sources of nitrogen (N), phosphorus (P) and dissolved organic carbon (DOC) to downstream aquatic ecosystems, and forest harvesting generally further increases the loading of DOC and nutrients. Continuous cover forestry (CCF) is proposed to be an environmentally more sustainable management option for peatland forests than conventional even-aged clear-cutting. However, the impacts of CCF on water quality, the biodegradability of DOC and consequent CO2 emissions from inland waters are poorly known. We studied the concentrations of N, P and DOC, the quality of DOC, and the mineralization of DOC to CO2 in ground water and ditch water in clear-cut, partially harvested, i.e. CCF, and uncut drained forests in Finland. Groundwater total N, NH4-N and PO4-P concentrations were significantly lower in CCF and uncut forest than in the clear-cut forest. Groundwater DOC concentrations were often highest in the clear-cut forest, where the water table was closer to the soil surface. Ditch water DOC and N concentrations were lowest next to the clear-cut area. DOC aromaticity in ground water was higher in the uncut forest than in the clear-cut and CCF, whereas ditch water aromaticity did not differ between the treatments. The biodegradation of DOC was studied by incubating water (at 15 °C for 24 h) 1, 3, 7 and 21 days after sampling. The results indicated that the majority of the CO2 production took place during the first three days, and CO2 fluxes were considerably higher from the ditch water than from the groundwater. The CO2 emissions were lower in summer than in the other seasons. Ditch water and groundwater CO2 production were generally significantly higher in the clear-cut than in the uncut forest. The results suggest that CCF can decrease the nutrient concentrations as well as CO2 emissions from inland waters compared to conventional clear-cutting.

Assessment of a portable UV-Vis spectrophotometer's performance in remote areas: Stream water DOC, Fe content and spectral data.

This paper presents data for the assessment of a portable UV-Vis spectrophotometer's performance on predicting stream water DOC and Fe content. The dataset contains DOC and Fe concentrations by laboratory methods, in-situ and ex-situ spectral absorbances, monitoring environmental indexes such as water depth, temperature, turbidity and voltage. The records in Yli-Nuortti river (Cold station, Finland) took place during the hydrological year 2018-2019 and in Krycklan (C4 and C5, Sweden) during the hydrological years 2016-2019. The data analyses were conducted with 'pls' and 'caret' package in R. The correlation coefficient (R), root-mean-square deviation (RMSD), standard deviation (STD) and bias were used to check the performance of the models. This dataset can be combined with datasets from other regions around the world to build more universal models. For discussion and more information of the dataset creation, please refer to the full-length article "Assessment of a portable UV-Vis spectrophotometer's performance for stream water DOC and Fe content monitoring in remote areas" [1].

Assessment of a portable UV-Vis spectrophotometer's performance for stream water DOC and Fe content monitoring in remote areas.

Quantification of dissolved organic carbon (DOC) and iron (Fe) in surface waters is critical for understanding the water quality dynamics, brownification and carbon balance in the northern hemisphere. Especially in the remote areas, sampling and laboratory analysis of DOC and Fe content at a sufficient temporal frequency is difficult. Ultraviolet-visible (UV-Vis) spectrophotometry is a promising tool for water quality monitoring to increase the sampling frequency and applications in remote regions. The aim of this study was (1) to investigate the performance of an in-situ UV-Vis spectrophotometer for detecting spectral absorbances in comparison with a laboratory benchtop instrument; (2) to analyse the stability of DOC and Fe estimates from UV-Vis spectrophotometers among different rivers using multivariate methods; (3) to compare site-specific calibration of models to pooled models and investigate the extrapolation of DOC and Fe predictions from one catchment to another. This study indicates that absorbances that were measured by UV-Vis sensor explained 96% of the absorbance data from the laboratory benchtop instrument. Among the three tested multivariate methods, multiple stepwise regression (MSR) was the best model for both DOC and Fe predictions. Accurate and unbiased models for multiple watersheds for DOC were built successfully, and these models could be extrapolated from one watershed to another even without site-specific calibration for DOC. However, for Fe the combination of different datasets was not possible.

Temperature sensitivity patterns of carbon and nitrogen processes in decomposition of boreal organic soils - Quantification in different compounds and molecule sizes based on a multifactorial experiment.

Climate warming and organic matter decomposition are connected in a recursive manner; this recursion can be described by temperature sensitivity. We conducted a multifactorial laboratory experiment to quantify the temperature sensitivity of organic carbon (C) and nitrogen (N) decomposition processes of common boreal organic soils. We incubated 36 mor and 36 slightly decomposed Carex-Sphagnum peat samples in a constant moisture and ambient temperature for 6 months. The experiment included three temperature and two moisture levels and two food web manipulations (samples with and without fungivore enchytraeid worms). We determined the release of carbon dioxide (CO2) and dissolved organic carbon (DOC) in seven molecular size classes together with ammonium N and dissolved organic N in low molecular weight and high molecular weight fractions. The temperature sensitivity function Q10 was fit to the data. The C and N release rate was almost an order of magnitude higher in mor than in peat. Soil fauna increased the temperature sensitivity of C release. Soil fauna played a key role in N release; when fauna was absent in peat, the N release was ceased. The wide range of the studied C and N compounds and treatments (68 Q10 datasets) allowed us to recognize five different temperature sensitivity patterns. The most common pattern (37 out of 68) was a positive upwards temperature response, which was observed for CO2 and DOC release. A negative downward pattern was observed for extractable organic nitrogen and microbial C. Sixteen temperature sensitivity patterns represented a mixed type, where the Q10function was not applicable, as this does not allow changing the sign storage change rate with increasing or decreasing temperature. The mixed pattern was typically connected to intermediate decomposition products, where input and output fluxes with different temperature sensitivities may simultaneously change the storage. Mixed type was typical for N processes. Our results provide useful parameterization for ecosystem models that describe the feedback loop between climate warming, organic matter decomposition, and productivity of N-limited vegetation.

Temperature sensitivity of soil organic matter decomposition after forest fire in Canadian permafrost region.

Climate warming in arctic/subarctic ecosystems will result in increased frequency of forest fires, elevated soil temperatures and thawing of permafrost, which have implications for soil organic matter (SOM) decomposition rates, the CO2 emissions and globally significant soil C stocks in this region. It is still unclear how decomposability and temperature sensitivity of SOM varies in different depths and different stages of succession following forest fire in permafrost regions and studies on long term effects of forest fires in these areas are lacking. To study this question, we took soil samples from 5, 10 and 30 cm depths from forest stands in Northwest Canada, underlain by permafrost, that were burnt by wildfire 3, 25 and over 100 years ago. We measured heterotrophic soil respiration at 1, 7, 13 and 19 °C. Fire had a significant effect on the active layer depth, and it increased the temperature sensitivity (Q10) of respiration in the surface (5 cm) and in the deepest soil layer (30 cm) in the 3-year-old area compared to the 25- and more than 100-year-old areas. Also the metabolic quotient (qCO2) of soil microbes was increased after fire. Though fires may facilitate the SOM decomposition by increasing active layer depth, they also decreased SOM quality, which may limit the rate of decomposition. After fire all of these changes reverted back to original levels with forest succession.

A synthesis of the impacts of ditch network maintenance on the quantity and quality of runoff from drained boreal peatland forests.

Drained peatlands are an important source of forest biomass in boreal regions and ditch network maintenance (DNM) operations may be needed to restore the drainage functions of ditches. By reviewing the available literature, as well as utilizing an existing hydrological model and analyzing the characteristics of eroded sediments, we assessed the impacts of DNM on runoff and exports of suspended solids (SS), dissolved organic carbon (DOC), nitrogen (N), and phosphorus (P). In general, DNM had minor impact on runoff and dissolved N and P, and it decreased rather than increased DOC exports. To increase the understanding of the hydrochemical impacts of DNM, future research should focus on the characteristics of SS and particulate nutrient exports. A major gap in knowledge is also the very limited regional representativeness of the available studies. High erosion risk in the ditches reaching the mineral soil below peat should be acknowledged when planning mitigation measures.

Post-fire carbon and nitrogen accumulation and succession in Central Siberia.

Improved understanding of carbon (C) accumulation after a boreal fire enables more accurate quantification of the C implications caused by potential fire regime shifts. We coupled results from a fire history study with biomass and soil sampling in a remote and little-studied region that represents a vast area of boreal taiga. We used an inventory approach based on predefined plot locations, thus avoiding problems potentially causing bias related to the standard chronosequence approach. The disadvantage of our inventory approach is that more plots are needed to expose trends. Because of this we could not expose clear trends, despite laborious sampling. We found some support for increasing C and nitrogen (N) stored in living trees and dead wood with increasing time since the previous fire or time since the previous stand-replacing fire. Surprisingly, we did not gain support for the well-established paradigm on successional patterns, beginning with angiosperms and leading, if fires are absent, to dominance of Picea. Despite the lack of clear trends in our data, we encourage fire historians and ecosystem scientists to join forces and use even larger data sets to study C accumulation since fire in the complex Eurasian boreal landscapes.

Runoff changes have a land cover specific effect on the seasonal fluxes of terminal electron acceptors in the boreal catchments.

Climate change influences the volume and seasonal distribution of runoff in the northern regions. Here, we study how the seasonal variation in the runoff affects the concentrations and export of terminal electron acceptors (i.e. TEAs: NO3, Mn, Fe and SO4) in different boreal land-cover classes. Also, we make a prediction how the anticipated climate change induced increase in runoff will alter the export of TEAs in boreal catchments. Our results show that there is a strong positive relationship between runoff and the concentration of NO3-N, Mn and Fe in agricultural catchments. In peaty catchments, the relationship is poorer and the concentrations of TEAs tend to decrease with increasing runoff. In forested catchments, the correlation between runoff and TEA concentrations was weak. In most catchments, the concentrations of SO4 decrease with an increase in runoff regardless of the land cover or season. The wet years export much higher amounts of TEAs than the dry years. In southern agricultural catchments, the wet years increased the TEA export for both spring (January-May) and autumn (September-December) periods, while in the peaty and forested catchments in eastern and northern Finland the export only increased in the autumn. Our predictions for the year 2099 indicate that the export of TEAs will increase especially from agricultural but also from forested catchments. Additionally, the predictions show an increase in the export of Fe and SO4 for all the catchments for the autumn. Thus, the climate induced change in the runoff regime is likely to alter the exported amount of TEAs and the timing of the export downstream. The changes in the amounts and timing in the export of TEAs have a potential to modify the mineralization pathways in the receiving water bodies, with feedbacks in the cycling of C, nutrients and metals in aquatic ecosystems.

Nitrogen balance along a northern boreal forest fire chronosequence.

Fire is a major natural disturbance factor in boreal forests, and the frequency of forest fires is predicted to increase due to climate change. Nitrogen (N) is a key determinant of carbon sequestration in boreal forests because the shortage of N limits tree growth. We studied changes in N pools and fluxes, and the overall N balance across a 155-year non stand-replacing fire chronosequence in sub-arctic Pinus sylvestris forests in Finland. Two years after the fire, total ecosystem N pool was 622 kg ha-1 of which 16% was in the vegetation, 8% in the dead biomass and 76% in the soil. 155 years after the fire, total N pool was 960 kg ha-1, with 27% in the vegetation, 3% in the dead biomass and 69% in the soil. This implies an annual accumulation rate of 2.28 kg ha-1 which was distributed equally between soil and biomass. The observed changes in N pools were consistent with the computed N balance +2.11 kg ha-1 yr-1 over the 155-year post-fire period. Nitrogen deposition was an important component of the N balance. The biological N fixation increased with succession and constituted 9% of the total N input during the 155 post-fire years. N2O fluxes were negligible (≤ 0.01 kg ha-1 yr-1) and did not differ among post-fire age classes. The number and intensity of microbial genes involved in N cycling were lower at the site 60 years after fire compared to the youngest and the oldest sites indicating potential differences in soil N cycling processes. The results suggest that in sub-arctic pine forests, the non-stand-replacing, intermediate-severity fires decrease considerably N pools in biomass but changes in soil and total ecosystem N pools are slight. Current fire-return interval does not seem to pose a great threat to ecosystem productivity and N status in these sub-arctic forests.

Spatial variability of mercury and polyunsaturated fatty acids in the European perch (Perca fluviatilis) - Implications for risk-benefit analyses of fish consumption.

This study evaluated the spatial variability of risks and benefits of consuming fish from humic and clear lakes. Mercury in fish is a potential risk for human health, but risk assessment may be confounded by selenium, which has been suggested to counterbalance mercury toxicity. In addition to the risks, fish are also rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), which are known to be beneficial for cardiovascular health and brain cognitive function in humans. We found that the concentrations of EPA + DHA and mercury in European perch (Perca fluviatilis) vary spatially and are connected with lake water chemistry and catchment characteristics. The highest mercury concentrations and the lowest EPA + DHA concentrations were found in perch from humic lakes with high proportion of peatland (30-50%) in the catchment. In addition, the ratio of selenium to mercury in perch muscle was ≥1 suggesting that selenium may counterbalance mercury toxicity. The observed variation in mercury and EPA + DHA content in perch from different lakes indicate that the risks and benefits of fish consumption vary spatially, and are connected with lake water chemistry and catchment characteristics. In general, consumption of perch from humic lakes exposed humans to greater risks (higher concentrations of mercury), but provided less benefits (lower concentrations of EPA + DHA) than consumption of perch from clear lakes.

Predicting the export and concentrations of organic carbon, nitrogen and phosphorus in boreal lakes by catchment characteristics and land use: A practical approach.

The majority of C, N and P in boreal lakes are in organic form. Organically bound nutrients are released through biodegradation or photodegradation which affects the water quality, eutrophication and greenhouse gas emissions of lakes. We tested whether open land-use data combined with land-use-specific export coefficients can be used to predict total organic carbon (TOC), dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP) loading and lake water concentrations. Using data from 12 lake catchments in eastern Finland, we found that land use and management of the catchment explained a substantial proportion of the variations in TOC (r 2 = 0.78), DON (r 2 = 0.55) and DOP (r 2 = 0.80) concentrations between lakes. The computation does not account for in-lake processes, which are reflected as mismatch between the predicted and observed concentrations. However, this simple practical approach is useful in ranking lakes according to their water quality. The results indicated that natural sources dominate TOC, DON and DOP exports; the background leachings accounted for 57-99 %, 48-96 % and 55-99 % of TOC, DON and DOP export, respectively. The proposed method has promise as a practical decision support tool for assessing the impacts of land use on water quality. The results showed that possibilities to control TOC, DON and DOP loading to surface waters are limited to catchments where the peatland proportion is low and anthropogenic sources significant.

A method to estimate the impact of clear-cutting on nutrient concentrations in boreal headwater streams.

Large-scale forestry operations, like clear-cutting, may impair surface water quality if not done with environmental considerations in mind. Catchment and country level estimates of nutrient loads from forestry are generally based on specific export values, i.e., changes in annual exports due to the implemented forestry operations expressed in kg ha(-1). We introduce here a specific concentration approach as a method to estimate the impact of clear-cutting on nutrient concentrations and export in headwater streams. This new method is potentially a more dynamic and flexible tool to estimate nutrient loads caused by forestry, because variation in annual runoff can be taken into account in load assessments. We combined water quality data from eight boreal headwater catchment pairs located in Finland and Sweden, where the effect of clear-cutting on stream water quality has been studied experimentally. Statistically significant specific concentration values could be produced for total nitrogen, nitrate, ammonium, and phosphate. The significant increases in the concentrations of these nutrients occurred between 2 and 6 years after clear-cutting. Significant specific concentration values could not be produced for total phosphorus and total organic carbon with the whole dataset, although in some single studies significant increases in their concentrations after clear-cutting were observed. The presented method enables taking into account variation in runoff, temporal dynamics of effects, and the proportional size of the treated area in load calculations. The number of existing studies considering large site-specific variation in responses to clear-cutting is small, and therefore further empirical studies are needed to improve predictive capabilities of the specific concentration values.

Nitrogen, phosphorus, carbon, and suspended solids loads from forest clear-cutting and site preparation: long-term paired catchment studies from eastern Finland.

The long-term impacts of current forest management methods on surface water quality in Fennoscandia are largely unexplored. We studied the long-term effects of clear-cutting and site preparation on runoff and the export of total nitrogen (total N), total organic nitrogen (TON), ammonium (NH(4)-N), nitrate (NO(3)-N), total phosphorus (total P), phosphate (PO(4)-P), total organic carbon, and suspended solids (SS) in three paired-catchments in Eastern Finland. Clear-cutting and soil preparation were carried out on 34 % (C34), 11 % (C11), and 8 % (C8) of the area of the treated catchments and wide buffer zones were left along the streams. Clear-cutting and soil preparation increased annual runoff and total N, TON, NO(3)-N, PO(4)-P, and SS loads, except for SS, only in C34. Runoff increased by 16 % and the annual exports of total N, TON, NO(3)-N, and PO(4)-P by 18, 12, 270, and 12 %, respectively, during the 14-year period after clear-cutting. SS export increased by 291 % in C34, 134 % in C11, and 16 % in C8 during the 14, 6, and 11-year periods after clear-cutting. In the C11 catchment, NO(3)-N export decreased by 12 %. The results indicate that while current forest management practices can increase the export of N, P and SS from boreal catchments for many years (>10 years), the increases are only significant when the area of clear cutting exceeds 30 % of catchment area.

Iron concentrations are increasing in surface waters from forested headwater catchments in eastern Finland.

Observations of increased water colour have been made in lakes and rivers all across the northern mid-latitudes of Europe and North America, particularly during the last 10-20 yr. This water browning or brownification has been attributed to the increased organic carbon concentrations due to climate change and decreased acid atmospheric deposition. Given that iron (Fe) may also increase water colour, the contribution of Fe to water brownification has received small attention. Our aim was to study the temporal trends of Fe in forested headwater catchments in eastern Finland, where an increasing air temperature and total organic carbon (TOC) trend had been observed in an earlier study. We found a statistically significant increasing trend also in stream water Fe concentrations and a strong correlation between the trends of TOC and Fe. The average increase in TOC and Fe concentrations between 1995 and 2006 was 0.5 mg l(-1) yr(-1) (2.5%), and 34.6 µ gl(-1) yr(-1) (3.5%), respectively. These results indicate that the increased water colour or brownification in Northern Europe may not only be due to increased concentrations of organic matter but also increased concentrations of Fe. The change in precipitation and temperature conditions, particularly during late autumn and early winter periods, appeared to be the main environmental factor behind increasing Fe trends. The strong correlation between the trends of Fe and TOC indicated that the increased Fe-organic matter complexation is the mechanism behind increasing Fe trends, but further research is needed to assess the chemical forms of increased Fe that coupled with increased TOC concentrations would enhance water brownification.

Trends in hydrometeorological conditions and stream water organic carbon in boreal forested catchments.

Temporal trends in stream water total organic carbon (TOC) concentration and export were studied in 8 forested headwater catchments situated in eastern Finland. The Seasonal Kendall test was conducted to identify the trends and a mixed model regression analysis was used to describe how catchment characteristics and hydrometeorological variables (e.g. precipitation, air and stream water temperatures, and atmospheric deposition) related to the variation in the concentration and export of stream water TOC. The 8 catchments varied in size from 29 to 494 ha and in the proportion of peatland they contained, from 8 to 70%. Runoff and TOC concentration were monitored for 15-29 years (1979-2006). Trends and variation in TOC levels were analysed from annual and seasonal time series. Mean annual TOC concentration increased significantly in seven of the eight catchments. The trends were the strongest in spring and most apparent during the last decade of the study period. The slopes of the trends were generally smaller than the variation in TOC concentration between years and seasons and between catchments. The annual TOC export showed no clear trends and values were largely determined by the temporal variability in runoff. Annual runoff showed a decreasing trend in two of the eight catchments. Mean annual air and stream water temperatures showed increasing trends, most clearly seen in the summer and autumn series. According to our modeling results, stream water temperature, precipitation and peatland percentage were the most important variables explaining annual and most seasonal TOC concentrations. The atmospheric deposition of SO4, NH4, and NO3 decreased significantly over the study period, but no significant link with TOC concentration was found. Precipitation was the main hydrometeorological driver of the TOC export. We concluded that stream water TOC concentrations and exports are mainly driven by catchment characteristics and hydrometeorological factors rather than trends in atmospheric acid deposition.