Habitat suitability of four threatened Himalayan species: Asiatic black bear, common leopard, musk deer, and snow leopard.

Background: Biodiversity conservation is becoming challenging day by day. For this, it is essential to understand the distribution, habitat, and impact of anthropogenic activities on animals at risk. We assessed the suitable habitats and anthropogenic impacts on Asiatic black bears, common leopards, musk deer, and snow leopards in and outside the protected areas of Gandaki Province, Nepal. Methods: We collected the presence locations of Asiatic black bears, common leopards, musk deer, and snow leopards based on scats and other signs. We employed the Maximum Entropy (MaxEnt) tool to identify suitable habitats of our studied species and their anthropogenic impacts on them. Results: The total suitable habitat of the common leopard was found to be 6,052 km2, followed by the Asiatic black bear (5,819 km2), snow leopard (4,447 km2), and musk deer (1,690 km2) in Gandaki Province. Most of the areas of suitable habitat for common leopards and Asiatic black bears were outside the protected areas, and for musk deer and snow leopards were inside the protected areas. Elevation was the most important variable determining habitat suitability of Asiatic black bear, common leopard, and musk deer, whereas the distance to water was the most important variable determining habitat suitability of snow leopard. Asiatic black bears, common leopards, and musk deer face significant anthropogenic impacts, but snow leopards face some anthropogenic impacts. Conclusion: Managing these animals' habitats inside and outside protected areas is essential. Hence, biodiversity conservation and livelihood opportunities should be balanced in the Himalayas on a win-win basis.

Predicting suitable habitat of swamp deer (Rucervus duvaucelii) across the Western Terai Arc Landscape of Nepal.

Over the last few years, intensifying human impact and the deterioration of natural habitats have severely restricted the global distribution of large herbivores. Rucervus duvaucelii, commonly recognized as the swamp deer, is a habitat-specialist endemic large herbivore of the Indian Subcontinent. It is classified as vulnerable by the IUCN and listed in CITES Appendix I due to a steep decline in its population, which is primarily due to anthropogenic causes. In Nepal, the last remaining population of this species is confined to limited pocket areas within the western Terai Arc Landscape. We explored potential habitat for swamp deer across this landscape using species distribution modelling through the MaxEnt algorithm by using 173 field-verified presence points alongside six anthropogenic, four topographic, and four vegetation-related variables. Our study found that out of the total study area (9207 km2), only 6% (590 km2) was suitable for swamp deer. Approximately 45% of suitable habitat was incorporated within protected areas, with Shuklaphanta National Park harboring the largest habitat patch. The suitability of habitat was discovered to be positively associated with low-elevation areas, areas near water sources, and areas far from settlements, implying the need to conserve water sources and minimize the extension of anthropogenic pressure for their long-term conservation. Additionally, we suggest the implications of a swamp deer-centric conservation strategy, with an emphasis on increasing connectivity through the corridors and landscape-level population connectivity through trans-boundary conservation initiatives between Nepal and India. Moreover, considering large herbivores' high vulnerability to extinction, similar researche incorporating anthropogenic factors is of the utmost importance to produce vital information on habitat suitability for conserving other regionally and globally endemic, habitat-specialized herbivores.

Potential risk zone for anthropogenic mortality of carnivores in Gandaki Province, Nepal.

Anthropogenic pressures in human-dominated landscapes often contribute to wildlife mortality. Carnivores are especially vulnerable to human-induced mortality due to the perceived threat to livestock and humans. Despite having widespread conservation implications, carnivore mortality data have been largely underutilized within Nepal. This study utilized Maxent to identify high-risk areas and explore the contribution of habitat attributes associated with carnivore mortality using the casualty database within the Gandaki province of central Nepal. We categorized the risk to carnivore species in three taxonomic groups, Felid, Viverridae, and Herpestidae, and identified a 3704-km2 area within the province at high risk for carnivore casualty. The middle mountains were the riskiest physiographic zone, and the Annapurna Conservation Area represented the largest risk zone among the four protected areas. Agricultural land was the most problematic area in terms of carnivore casualty. The human population was positively associated with high-risk areas and the number of casualties, whereas protected area cover had a negative association. This study identified that the common leopard was at the highest risk of mortality and therefore would benefit from the implementation of an action plan and species-specific conservation strategies, especially within identified high-risk zones. An expansion of protected areas in the middle mountain region would serve to greatly reduce carnivore casualty. Species distribution modeling can be further used with national-level spatial and temporal mortality data to identify the most prominent casualty times and pinpoint potential casualty locations throughout the country.

Factors influencing the global distribution of the endangered Egyptian vulture.

Vultures are ecologically important primarily because of their scavenging role in cleaning carcasses of the environment. Because of anthropogenic impacts, the Egyptian vulture (Neophron percnopterus) has suffered catastrophic declines in parts of its range and, thus, information about its global distribution and factors influencing its occurrence within this range are essential for its conservation. To this end, we estimated the global distribution of Egyptian vulture and variables related to this distribution. We used occurrence points (n = 4740) from online data sources and literature, environmental variables related to these sites and Maximum Entropy software to model the distribution of this species and its relationship to environmental variables during the entire year, breeding and overwintering. Out of ~ 49 million km2 study area, the Egyptian vulture had a predicted range of 6,599,508 km2 distributed across three continents: Africa, Asia and Europe. The densest distribution was in Southern Europe, India and Northern Africa and a sparser distribution was around Mid and Western Africa, the Middle East and Afghanistan. Climate was related to the vulture's most probable range: in particular medium temperature seasonality and low precipitation during the coldest yearly quarter were important variables regardless of the season of observations examined. Conservation of identified habitats and mitigation of anthropogenic impacts to conserve these vultures are recommended for immediate and long-term conservation of the Egyptian vulture globally.

Factors affecting livestock depredation by snow leopards (Panthera uncia) in the Himalayan region of Nepal.

The snow leopard (Panthera uncia) found in central Asia is classified as vulnerable species by the International Union for Conservation of Nature (IUCN). Every year, large number of livestock are killed by snow leopards in Nepal, leading to economic loss to local communities and making human-snow leopard conflict a major threat to snow leopard conservation. We conducted formal and informal stakeholder's interviews to gather information related to livestock depredation with the aim to map the attack sites by the snow leopard. These sites were further validated by district forest office staffs to assess sources of bias. Attack sites older than 3 years were removed from the survey. We found 109 attack sites and visited all the sites for geo location purpose (GPS points of all unique sites were taken). We maintained at least a 100 m distance between attack locations to ensure that each attack location was unique, which resulted in 86 unique locations. A total of 235 km2 was used to define livestock depredation risk zone during this study. Using Maximum Entropy (MaxEnt) modeling, we found that distance to livestock sheds, distance to paths, aspect, and distance to roads were major contributing factors to the snow leopard's attacks. We identified 13.64 km2 as risk zone for livestock depredation from snow leopards in the study area. Furthermore, snow leopards preferred to attack livestock near livestock shelters, far from human paths and at moderate distance from motor roads. These identified attack zones should be managed both for snow leopard conservation and livestock protection in order to balance human livelihoods while protecting snow leopards and their habitats.

Ecological status of a freshwater tectonic lake of the indo-burmese province: Implications for livelihood development.

Tectonic lakes are among the most geologically fascinating and environmentally versatile hydrobiological systems found on the earth's surface. We conducted a study on the limnology of Tasek Lake, a tectonic lake located in the Indo-Burma Province of the South Asian region. Physico-chemical parameters of the lake's water along with its plankton were considered for the study. Their relationship was analysed by understanding their seasonal variations and through linear regression models. The water quality index (WQI), plankton diversity indices and canonical correspondence analysis (CCA) were computed. The ichthyofaunal diversity was also studied to get an insight into the lake's fishery potential. A preliminary assessment on the economic feasibility of converting Tasek Lake into a fishery was also completed. Results indicate moderate eutrophication in the lake and the plankton population is observed to be rich and abundant. The WQI value confirms the water to be of "very poor" quality. The CCA was done to analyze the relationships of physico-chemical parameters with months and seasons, and the relation between seasons and plankton assemblages. Results corroborate the results of WQI. Identified fish population suggest ample fishery potential of the lake. The economic assessment reveals that in order to maintain the ecological sustainability of the lake, it should be transformed into a recreational fishery, following a catch-and-release model. The study calls for urgent restoration of the lake so that not only its pristine ecology is survived but also its fishery potential is sustainably harnessed and local livelihood is improved.

Suitable habitat of wild Asian elephant in Western Terai of Nepal.

BACKGROUND: There is currently very little available research on the habitat suitability, the influence of infrastructure on distribution, and the extent and connectivity of habitat available to the wild Asian elephant (Elephas maximus). Information related to the habitat is crucial for conservation of this species. METHODS: In this study, we identified suitable habitat for wild Asian elephants in the Western Terai region of Nepal using Maximum Entropy (MaxEnt) software. RESULTS: Of 9,207 km2, we identified 3194.82 km2 as suitable habitat for wild Asian elephants in the study area. Approximately 40% of identified habitat occurs in existing protected areas. Most of these habitat patches are smaller than previous estimations of the species home range, and this may reduce the probability of the species continued survival in the study area. Proximity to roads was identified as the most important factor defining habitat suitability, with elephants preferring habitats far from roads. CONCLUSIONS: We conclude that further habitat fragmentation in the study area can be reduced by avoiding the construction of new roads and connectivity between areas of existing suitable habitat can be increased through the identification and management of wildlife corridors between habitat patches.

An assessment of human impacts on endangered red pandas (Ailurus fulgens) living in the Himalaya.

Anthropogenic factors play an important role in shaping the distribution of wildlife species and their habitats, and understanding the influence of human activities on endangered species can be key to improving conservation efforts as well as the implementation of national strategies for sustainable development. Here, we used species distribution modeling to assess human impacts on the endangered red panda (Ailurus fulgens) in high-altitude regions of Nepal. We found that the distance to paths (tracks used by people and animals), livestock density, human population density, and annual mean temperature were the most important factors determining the habitat suitability for red pandas in Nepal. This is the first study that attempts to use comprehensive environmental and anthropogenic variables to predict habitat suitability for the red pandas at a national level. The suitable habitat identified by this study is important and could serve as a baseline for the development of conservation strategies for the red panda in Nepal.

Diet and macronutrient niche of Asiatic black bear (Ursus thibetanus) in two regions of Nepal during summer and autumn.

Relatively little is known about the nutritional ecology of omnivorous Asiatic black bears (Ursus thibetanus) in Nepal. We characterized the diet of black bears in two seasons (June-July, "summer"; and October-November "autumn") and two study areas (Dhorpatan Hunting Reserve [DHR]; and Kailash Sacred Landscape [KSL]). We then conducted nutritional analysis of species consumed by black bears in each study area, in combination with nutritional estimates from the literature, to estimate the proportions of macronutrients (i.e., protein [P], lipid [L], and carbohydrate [C]) in the seasonal bear foods and diets, as well as their macronutrient niche breadth. We found that bamboo (Arundinaria spp.) had the highest relative frequency in both study areas and seasons. Ants and termites were found in DHR diets, but not KSL diets. One anthropogenic crop was found in DHR summer diets (Zea mays) and two were found in KSL summer diets (Z. mays; and Kodo millet [Paspalum scrobiculatum]). Other than insects, no animal prey was found in either diet. The proportions of macronutrients in diets (i.e., realized macronutrient niches) were relatively high in carbohydrate for both study areas and seasons: DHRsummer 24.1P:8.7L:67.2C; KSLsummer 16.7P:8.2L:75.1C; DHRautumn 21.1P:10.5L:68.4C; KSHautumn 19.0P:11.0L:70.0C. Macronutrient niche breadth was 3.1 × greater in the DHR than KSL during summer, and 4.0 × greater in the autumn, primarily due to the higher proportion of lipid in ants and termites relative to plant foods. Within-study area differences in niche breadth were greater during summer than autumn; in the KSH the macronutrient breadth was 1.4 × greater in summer, while in the DHR it was 1.1 × greater in summer. Similarity in dietary macronutrient proportions despite differences in foods consumed and niche breadth are suggestive of foraging to reach a preferred macronutrient balance.

Habitat overlap between Asiatic black bear Ursus thibetanus and red panda Ailurus fulgens in Himalaya.

Studying habitat overlap between sympatric species is one of the best ways to identify interspecies relationships and to direct conservation efforts so that multiple species can benefit. However, studies exploring interspecies relationships are very limited in Nepal, making it difficult for the government of Nepal and conservation partners to manage wildlife in their habitats, especially in Himalayan protected areas. In this study, we identified habitat overlap between Asiatic black bear (Ursus thibetanus) and red panda (Ailurus fulgens) as well as important habitat types for both species in the Makalu Barun National Park, Nepal using Maximum Entropy (MaxEnt) modeling. GPS points of species occurrence were collected from the field, and environmental variables were extracted from freely available sources. We found that the study area contained 647 km2 of Asiatic black bear habitat and 443 km2 of the red panda habitat. 368 km2 supported both species, which constituted 57% of the Asiatic black bear habitat and 83% of the red panda habitat. We found that conifer forest was the most important habitat type for both species. Because the largest portions of both species' habitat were located inside the buffer zone, a peripheral zone of national park, conservation efforts for these sympatric species should be focused inside the buffer zone to be most effective.

Dry season diet composition of four-horned antelope Tetracerus quadricornis in tropical dry deciduous forests, Nepal.

It is essential to assess the feeding strategies of threatened species during resource-scarce seasons to understand their dietary niche breadth and inform appropriate habitat management measures. In this study, we examined the diet composition of four-horned antelope (FHA) Tetracerus and quadricornis, one of the least studied ungulate species, in Banke National Park, Nepal. A total of 53 fresh pellet groups were collected between December 2015 and January 2016 and analyzed using micro-histological fecal analysis technique. First, we prepared 133 micro-histological photographs of different parts of 64 reference plant species. Then we compared 1,590 fragments of 53 fecal samples with photographs of reference plants to assess the percentage of occurrence of different plant species in FHA diet. A total of 30 plant species belonging to 18 different families were identified in fecal samples. Chi-square goodness of fit tests showed that FHA appeared not to feed all plant uniformly. Out of 1,520 identified fragments in fecal samples, 1,300 were browse species and 220 were grass species. Browse represented 85.5% of the identified plant fragments, suggesting that FHA might be adopting a browser strategy at least during winter when grasses are low in abundance and their nutritive quality is poor. Tree species had the highest contribution in the diet (46.55%) followed by shrubs (24.52%). The family Gramineae was consumed in the highest proportion (27.68%) followed by Euphorbiaceae (11.95%). Overall, our results suggest that FHA has the feeding plasticity to adapt to resource fluctuation. Based on the findings of this study, we recommend that dicot plant species-particularly fruit trees and shrubs, which are the major source of nutrients for FHA during resource-lean, dry season-be conserved and natural regeneration of these taxa be promoted.

Large anthropogenic impacts on a charismatic small carnivore: Insights from distribution surveys of red panda Ailurus fulgens in Nepal.

Protected areas are key to preserving biodiversity and maintaining ecosystem services. However, their ability to ensure long-term survival of threatened andendangered species varies across countries, regions and landscapes. Distribution surveys can beparticularly important for assessing the value of protected areas, and gauging their efficacy incatering to species-specific requirements. We assessed the conservation value of one such reserve for a charismatic yet globally endangered species, the red panda Ailurus fulgens,in the light of on-going land-use transformation in Nepal. We conducted field surveys forindirect signs of red pandas along forest trails in 25-km2 sampling grid cells (n = 54) of Dhorpatan Hunting Reserve, and confronted a set of ecological hypotheses to the data using hierarchical occupancy models. We estimated overall occupancy at Ψ(SE) = 0.41 (0.007), with relatively high site-level detectability [p = 0.93 (SE = 0.001)]. Our results show that despitebeing a subsistence form of small-scale resource use, extraction of bamboo and livestock grazing negatively affected panda occurrence, albeit at different intensities. The amount of bamboo cover,rather than the overall proportion of forest cover, had greater influence on the panda occurrence. Despite availability of bamboo cover, areas with bamboo extraction and anthropogenic disturbances were less likely to be occupied by pandas. Together, these results suggest that long-term persistence of red pandas in this reserve and elsewhere across the species' range will require preventing commercial extractionof bamboo, coupled with case-specific regulation of anthropogenic exploitation of red panda habitats.

Diet and nutrient balance of red panda in Nepal.

We identified the winter plant species consumed by red panda in the Dhorpatan Hunting Reserve of eastern Nepal and compared this to the early-summer diet which was determined previously by Panthi et al. (2012). In addition, we estimated the proximate nutritional content of the leaves identified in red panda diet for both seasons, and we used nutritional geometry to explore macronutrient balance of leaves from the two different sampling periods. We identified six different plants in winter scats, which were the same as found in the previously determined early-summer diet. Arundinaria spp. bamboos were the main species found (82.1 % relative frequency), followed by Acer spp. (6.3 %), Betula utilis (4.6 %), Quercus semicarpifolia (3.7 %), Berberis spp. (1.3 %), and lichens (1.0 %), leaving 2.0 % unidentified. Geometric analysis suggested that the macronutrient balance of seasonal diets were similar in nutrient balance to the most frequently consumed Arundinaria spp. Differences in macronutrient balance may indicate seasonal nutrient preferences, such as increased carbohydrate intake in winter for thermogenesis, and increased protein and lipid intake in early summer to support reproduction and lactation; however, these differences may also indicate differences in resource availability. Habitat conserved for red panda in the region should include sufficient Arundinaria spp. as well as lesser consumed plants which may serve as complimentary foods.