RESUMO
Introduction: Due to its biological and antibacterial qualities, many plants, including curcumin, are used as phytomedicines in dentistry. They are primarily used as intracanal medication in endodontics to prevent probable chemical side effects and also to address antimicrobial resistance. Curcumin nanoformulations have improved antibacterial activity and improved dispersion, making them the superior form of curcumin. The purpose of this study was to assess curcumin and nanocurcumin's antibacterial properties. As a gutta-percha coating, they are to be tested against Escherichia coli. Materials and Methods: The study employs the standard strain of E. coli, ATCC 25922. The antibacterial activity of gutta-percha cones against E. coli is assessed after coating them with suspensions of curcumin and nanocurcumin. Scanning electron microscopy is utilized to evaluate the coatings' continuity. Results: The gutta-percha cones that are untreated, coated with curcumin, and coated with nanocurcumin exhibit significantly different levels of antibacterial activity. There is statistically significant variation in their antibacterial activity. Conclusion: (1) Compared to curcumin-coated and untreated gutta-percha cones, those coated with nanocurcumin exhibit a stronger antibacterial activity. (2) Compared to uncoated gutta-percha cones, gutta-percha cones coated with curcumin exhibit more antibacterial action.
RESUMO
Background: Nanocurcumin has antimicrobial properties and it is to be tested as a coating on gutta-percha against Enterococcusi faecalis. Aim: To evaluate the antimicrobial efficacy of nanocurcumin-coated gutta-percha against E. faecalis in comparison with conventional gutta-percha. Materials and Methods: The broth dilution method and colony-forming unit count assay were chosen for the evaluation of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration of nanocurcumin against E. faecalis. ISO size 30, 4% taper gutta-percha cones were manually coated with nanocurcumin. All the coated and noncoated gutta-percha cones were examined under a scanning electron microscope to study the exterior surface. Antibacterial efficacy of nanocurcumin-coated gutta-percha and conventional gutta-percha was seen by agar diffusion method against E. faecalis. Results: MIC of nanocurcumin was observed at 50 mg/ml for E. faecalis. Nanocurcumin-coated gutta-percha showed a larger zone of inhibition when compared to conventional gutta-percha which showed a smaller zone of inhibition (P < 0.0001). Nanocurcumin-coated gutta-percha showed moderate antimicrobial activity, while conventional gutta-percha showed weak activity. Conclusion: The results of the study reveal that nanocurcumin has an antimicrobial activity against E. faecalis. The use of herbal alternatives in endodontics might prove to be advantageous.
RESUMO
Background: The persistence of microorganisms in root canal system is pivotal factor pertinent to endodontic failure. Even if you meet the highest technical and asepsis standards and also minimize the procedural errors, failures result. The rapidly increasing antibiotic resistance among these bacteria and the adverse effects of these antibiotics along with their toxicity are the main situations indicating the utmost urgent requirement of a safe, effective, natural phytochemical like curcumin with tremendous medicinal potential. Nanoformulations of curcumin are their improved version with enhanced antibacterial activity. Materials and Methods: A thin layer of nanocurcumin was coated on the surface of gutta-percha cones. To observe the uniformity and adherence of nanocurcumin coating on the exterior surface of gutta-percha, scanning electron microscopy (SEM) was done. Further agar gel diffusion technique was used to assess the antimicrobial activity of nanocurcumin-coated gutta-percha cones and conventional gutta-percha cones and their results were compared statistically. Results: The results of SEM study showed a layer of nanocurcumin adhering uniformly to the surface of gutta-percha cones. Furthermore, the nanocurcumin-coated gutta-percha cones demonstrated higher antibacterial activity as compared to the conventional cones. Conclusions: Our study results reveal that the coating of nanocurcumin on gutta-percha cones has augmented their antibacterial activity.
