Protective factors for caries of a school-based oral health program in Bangkok, Thailand: a retrospective cohort study.

OBJECTIVES: The aim of the study was to assess protective factors of a school-based oral health preventive program on caries in permanent teeth in students and to identify other risk and protective factors associated with caries in this group. STUDY DESIGN: This is a retrospective cohort study. METHODS: A total of 433 students were examined in the 1st grade and followed up until the 6th grade. Each student was annually assessed for caries as per the World Health Organization index. Binary simple and multiple logistic regressions were performed to assess risk or protective factors with a P-value ≤0.05. RESULTS: In 1st graders, the caries prevalence in deciduous teeth was 87.2%, and the mean decayed, missing and filled teeth (dmft) index was 6.44 (±4.4); in 6th graders, the caries prevalence in permanent teeth was 51.7%, and the mean DMFT was 1.37 (±1.84). Protective factors for caries in permanent teeth were complete participation in the program, receiving fluoride varnish >4 times, and sealant applications on all first molars. Risk factors were female sex and caries prevalence in deciduous teeth. After multiple logistic regression, sealant application only remained significant, with an odds ratio (OR) of 0.19 (95% confidence interval [CI] = 0.06-0.63, P = 0.007), and for risk factor, caries prevalence in deciduous teeth only remained significant, with an OR of 5.44 (95% CI = 2.23-13.27, P < 0.001). CONCLUSIONS: There is an association between deciduous dentition caries and permanent dentition caries. Early prevention in school settings by applying fluoride varnish and dental sealants onto molars is protective and feasible. The study suggests that regular fluoride varnish and sealant applications should be extended to similar low-income area schools.

Clock gene expression levels and relationship with clinical and pathological features in colorectal cancer patients.

The clock gene machinery controls cellular metabolism, proliferation, and key functions, such as DNA damage recognition and repair. Dysfunction of the circadian clock is involved in tumorigenesis, and altered expression of some clock genes has been found in cancer patients. The aim of this study was to evaluate the expression levels of core clock genes in colorectal cancer (CRC). Quantitative real-time polymerase chain reaction (qPCR) was used to examine ARNTL1, CLOCK, PER1, PER2, PER3, CRY1, CRY2, Timeless (TIM), TIPIN, and CSNK1? expression levels in the tumor tissue and matched apparently healthy mucosa of CRC patients. In the tumor tissue of CRC patients, compared to their matched healthy mucosa, expression levels of ARNTL1 (p=.002), PER1 (p=.002), PER2 (p=.011), PER3 (p=.003), and CRY2 (p=.012) were lower, whereas the expression level of TIM (p=.044) was higher. No significant difference was observed in the expression levels of CLOCK (p=.778), CRY1 (p=.600), CSNK1 (p=.903), and TIPIN (p=.136). As to the clinical and pathological features, a significant association was found between low CRY1 expression levels in tumor mucosa and age (p=.026), and female sex (p=.005), whereas high CRY1 expression levels in tumor mucosa were associated with cancer location in the distal colon (p?=?.015). Moreover, high TIM mRNA levels in the tumor mucosa were prevalent whenever proximal lymph nodes were involved (p= .013) and associated with TNM stages III-IV (p=.005) and microsatellite instability (p=.015). Significantly poorer survival rates were evidenced for CRC patients with lower expression in the tumor tissue of PER1 (p=.010), PER3 (p= .010), and CSNKIE (p=.024). In conclusion, abnormal expression levels of core clock genes in CRC tissue may be related to the process of tumorigenesis and exert an influence on host/tumor interactions.

Acute aortic dissection in the young: clinical series.

Acute aortic dissection (AAD) is an uncommon lethal cardiovascular emergency demanding prompt diagnosis and aggressive therapeutic intervention. Although it usually affects males over 60 years of age, it may also occur in young adults with specific risk factors such as Marfan syndrome, bicuspid aortic valve and larger aortic dimensions. Moreover, it should be underlined that it is frequently associated with unusual presentation and that the mortality risk is similar to older AAD patients. Thus ''a call to arms'' of the medical community is needed to better understand the spectrum of acute aortic syndromes and to define appropriate diagnostic and therapeutic pathways. We report a series of 6 young patients with type A - AAD referred over the past years at our institute.

Idiopathic isolated coronary ostial stenosis: a rare lesion with particular clinical and surgical implications.

Isolated coronary ostial stenosis is an unusual condition, most often atherosclerotic in origin. Only few cases have been reported as idiopathic. The clinical profile of the latter patients suggests that this group may represent a distinct clinical entity, with peculiar diagnostic and therapeutic features. We present our experience concerning six patients with isolated idiopathic coronary ostial stenosis, who successfully underwent transaortic left main ostioplasty with autologous pericardial patch fixed in glutaraldehyde. A review of previous reports in regard to the surgical management of coronary ostial disease is presented.

Total anomalous left pulmonary venous connection with intact atrial septum: surgical treatment of a rare case.

Total anomalous left pulmonary venous connection with intact atrial septum is an extremely rare form of congenital heart disease. We describe a case never reported before in which the left pulmonary veins drained directly into the right atrium through a common vein. Surgical correction was successfully obtained by redirecting the blood flow through a newly created atrial septal defect into the left atrium using a gluteraldehyde-treated autologous pericardial patch. Follow-up at 6 months shows no signs of any complication.

Massive pulmonary embolism 3 hours after cardiopulmonary bypass. An exceeding rare case.

A 70-year-old woman underwent an aortocoronary bypass. Three hours later, she experienced severe pulmonary embolism, diagnosed by transesophageal echocardiography and followed by cardiac arrest. Resuscitation maneuvers were unsuccessful. Autopsy confirmed the diagnosis. When pulmonary embolism occurs after cardiopulmonary bypass for cardiac surgery, it usually occurs in the 2nd postoperative week; and to the best of our knowledge, the literature contains no other reports of cases that occurred during a shorter postoperative interval. Prevention of pulmonary embolism in high-risk patients is mandatory. When embolism occurs, transesophageal echocardiography is an essential tool in making the diagnosis and in guiding the surgeon during intervention.

Primary malignant melanoma of the oesophagus with a left atrial metastasis.

A 75 year old man with an eight month history of dysphagia and weight loss underwent pericardiocentesis for a massive pericardial effusion. The echocardiogram showed a mass in the left atrium, and computed tomography also showed distal oesophageal narrowing, which was found to be due to a malignant melanoma. The left atrial mass, investigated by immunoscintigraphy with technetium-99m labelled monoclonal antibody, was diagnosed as metastatic melanoma. This represents a rare case of primary oesophageal melanoma with left atrial metastasis.

Clinical experience with the transvenous Medtronic Pacer Cardioverter Defibrillator (PCD) System.

We review our experience with the transvenous Medtronic Pacer Cardioverter Defibrillator System (Model 7217B), a multifunction implantable pacer defibrillator combined with a transvenous lead system (Transvene). From April 1991 to October 1992, we implanted this device in 19 consecutive patients (11 men and 8 women; average age, 56.5 years). Nine patients (47.4%), 5 with coronary artery disease and 4 with dilated cardiomyopathy, had an ejection fraction of < 30%. The average operative time was 129 minutes. In 18 patients (94.7%), the transvenous lead system provided effective sensing, pacing, and defibrillation during intraoperative testing. In each of these cases, the defibrillation threshold was less than 18 J. In 1 patient (5.3%), it was necessary to switch to epicardial leads, which were implanted through a left thoracotomy. All patients were extubated in the recovery room. The average hospital stay was 8 days. There was no early mortality or morbidity. During a maximum follow-up period of 17 months (mean, 9.2 months), no sudden death occurred. The implantable system terminated 245 ventricular tachycardia episodes in 14 patients (73.7%) and 82 ventricular fibrillation episodes in 13 patients (68.4%). Two hundred eleven (86.1%) of the ventricular tachycardia episodes were resolved by antitachycardia pacing alone. In 2 patients (10.5%), the caval electrode became dislocated; repositioning of the electrode was followed by repeat defibrillation threshold evaluation. Our experience shows that the transvenous Medtronic Pacer Cardioverter Defibrillator System provides safe, effective treatment of ventricular tachyarrhythmias. Because the perioperative mortality and morbidity are extremely low, use of this device may be particularly beneficial in patients with a high operative risk. Moreover, the lower number of unpleasant therapeutic shocks should increase patient a acceptance of the device.

One-year monkey heart xenograft survival in cyclosporine-treated baboons. Suppression of the xenoantibody response with total-lymphoid irradiation.

The shortage of cadaveric human organs for transplantation could be alleviated by the use of xenografts. Long-term (> one-year) survival of xenografts in humans or experimental animals has not been achieved with previous immunosuppressive protocols. Poor results in xenotransplantation compared with allotransplantation have been attributed to a more potent antibody response rather than to cell-mediated responses. To investigate these issues a concordant heterotopic cardiac xenograft model was developed in conjunction with cyclosporine and/or total-lymphoid irradiation. Concordant models permit examination of xenoantigen induced antibody and cell-mediated responses since preformed humoral factors (in discordant models) do not cause hyperacute rejection. Four groups of baboon recipients received cervical heart transplants from cynomolgous monkeys. Group I (n = 2), untreated, mean survival (MS) = 6 days; group II (n = 5), CsA and methylprednisolone, MS = 25 days; group III (n = 3), preoperative TLI, MS = 29 days; group IV (n = 6), preoperative TLI and CsA+MP, MS = 255 days (> 77, > 108, > 142, 184, > 480, 540 days). Heart xenografts of CsA-MP-treated recipients appear to be destroyed predominantly by antibody (IgM)-mediated processes whereas cell-mediated rejection is likely the major reaction in TLI-treated recipients. CsA-MP-treated recipients had early immunohistochemical evidence of antibody and complement-mediated rejection (deposition of IgM and complement but not IgG on heart xenografts). In contrast IgM and complement deposits were not detected on heart xenografts in TLI- and TLI-CsA-treated recipients. IgG xenoantibodies were only detected on the two rejected heart grafts of TLI-CsA-treated recipients. CsA-MP-treated recipients rapidly developed high xenoantibody titers (1:256 to 1:512) that immediately preceded rejection. In contrast, TLI-treated animals developed lower levels of xenoantibody (< or = 1:8) and TLI-CsA-treated recipients had no detectable xenoantibody during the initial three months after transplantation (and titers no greater than 1:8 thereafter.) The lack of xenoantibody was likely not due to a generalized inhibitory effect of the immunosuppressants on B cell function since all classes of serum immunoglobulins were in the normal range. Intragraft cytolytic lymphocyte activity was detected in rejecting TLI- and TLI-CsA-treated recipients but could not be detected in xenografts of CsA-MP-treated recipients. One explanation for these data is that TLI (either directly or indirectly) induces a state of specific B cell unresponsiveness to monkey xenoantigens, thereby preventing IgM mediated rejection in the third week after transplantation.(ABSTRACT TRUNCATED AT 400 WORDS)

Effect of complete cardiac denervation on atrial natriuretic factor release in baboons.

We investigated the influence of cardiac innervation on atrial natriuretic factor (ANF) release in baboons. For this purpose, plasma ANF levels were measured in control conditions and in response to head-down (-45 degrees) and head-up tilt (+45 degrees) in six anesthetized baboons before and after complete cardiac denervation obtained by orthotopic autotransplantation of the heart. Cardiac denervation did not modify baseline plasma ANF levels (60.4 +/- 17 pg/ml before and 63.1 +/- 16 pg/ml after heart autotransplantation). In contrast the significant ANF responses to changes in central venous pressure (CVP) induced by postural maneuvers (-45 degrees, + 16.2 +/- 4 pg/ml; +45 degrees, -18.5 +/- 4 pg/ml) were markedly altered after cardiac denervation (-45 degrees, +5.8 +/- 2 pg/ml; +45 degrees, -7.6 +/- 1 pg/ml). The changes in CVP and systemic blood pressure evoked by the postural challenges were comparable before and after cardiac denervation. These results demonstrate that cardiac nerves play a role in the control of ANF release.

Ten questions to ask about revolving drug funds.

For any health financing scheme, the key issue is how to raise sufficient resources to provide a reasonable service when poverty means that many people are unable to afford such a service. A revolving drug fund is a scheme where drugs are sold at cost-price, plus a mark-up, and the revenue is used to replenish the drug stocks. Probably the most difficult part of establishing an RDF is setting prices. There should be sufficient mark-up to cover exempt treatments, transport costs, inflation and currency devaluations. Adequate records must be kept. If the amount of money and/or drugs in the RDF appears to be declining, the reason can be established. RDFs should not be seen in isolation from other considerations of the health care system. Drugs should not be sold without an adequate check on prescribing habits. Care should also be taken that the money-making potential of drugs does not distract from preventive activities.

Successful cardiac xenogeneic transplantation in primates using total lymphoid irradiation.

Combined total lymphoid irradiation (TLI) and cyclosporine (CyA) dramatically prolong cardiac xenografts in small animals. In order to evaluate this immunosuppressive regimen in primates, heterotopic cardiac cervical xenografts were implanted in a monkey-to-baboon model. The following groups evolved: group 1 (n = 2) control, no immunosuppression; group 2 (n = 4) CyA and steroids; group 3 (n = 2) preoperative TLI (800 rad total); group 4 (n = 4) TLI combined with CyA and steroids as in groups 2 and 3. Complement-dependent cytotoxicity, mixed lymphocyte culture, cell mediated cytotoxicity as well as myocardial biopsies were periodically monitored. A Muga scan was performed at 1 year posttransplant to assess ventricular function. Cardiac xenograft survival was best in group 4 animals (108, 184, 480, 540 days) compared with 5 and 7 days in the control group. In group 2, graft survival was 13, 17, 18 and 63 days. TLI alone prolonged survival up to 28 and 29 days. Hemorrhage and myocyte necrosis were seen in all rejected grafts of group 1, 2 and 3 animals. Mononuclear cell infiltrate and fibrosis were present in group 4 grafts at rejection. High antibody titers (1:256 to 1:512) were detected at rejection only in group 1, 2 and 3 animals. This suggests that the combined use of TLI and CyA substantially prolongs xenograft survival in a primate model by preventing early antibody-mediated rejection as well as by limiting cellular response.

Myocardial revascularization in women.

To compare the results of coronary artery bypass in women and men, we reviewed our experience from January 1976 through June 1989. During this period, 170 women with coronary artery disease but with no other cardiac abnormalities underwent coronary artery bypass. We compared this group with a group of 150 men, matching them according to age, presence of angina, extent of disease, and surgical treatment. Preoperative clinical features, surgical data, and early and late results were analyzed. The operative mortality was similar between groups (2.9% for women vs 2.6% for men). The women, however, were more frequently overweight (54% vs 15%; p <0.001) and more often had the following: diabetes mellitus (34% vs 20%; p <0.01), a coronary artery diameter of <1.8 mm (64% vs 29%; p <0.001), poor saphenous vein quality (50% vs 16%; p <0.001), and incomplete revascularization (20% vs 4%; p <0.001). After a mean follow-up of 6 years, the women also had a higher incidence of recent-onset myocardial infarction (31% vs 12%; p <0.001) and a greater tendency to be symptomatic (48% vs 19%; p <0.001). The 12-year cumulative survival rates were similar in both groups (76.2% for women vs 77.1% for men). According to logistic regression analysis of the significantly different variables, the only independent determinants of postoperative asymptomatic status were satisfactory coronary artery caliber, good saphenous vein quality, and complete revascularization. We conclude that poorer functional results after coronary artery bypass surgery in women may be caused by a poorer quality of revascularization, which in turn is a result of smaller coronary artery diameter, worse distal runoff, and less satisfactory vein quality.

Primate cardiac allo-and xenotransplantation: modulation of the immune response with photochemotherapy.

Survival of heterotopic allo- and xenografts was studied in a primate cardiac transplantation model. Initially, animals were presensitized to donor xenoantigens by blood transfusion and treated with strategies designed to elicit pretransplant immunosuppression. These animals underwent rapid rejection whether treated with ciclosporin and conventional immunosuppression, autologous sera, sensitized lymphocytes, or photochemotherapy. In a nonsensitized xenograft study, xenografts were maintained for prolonged periods with a combination of ciclosporin and photochemotherapy. All animals treated with photochemotherapy demonstrated periodic suppression of the mixed leukocyte culture response to their donor. Inhibition of lymphocytotoxic antibody was achieved only in animals treated with both ciclosporin and photochemotherapy. Xenosensitized animals were secondarily exposed to donor alloantigens. These animals were successfully allografted. Ciclosporin therapy was terminated and the animals maintained by photochemotherapy alone. This preliminary trial indicates that a combination of ciclosporin and photochemotherapy may be of value in maintaining concordant xenografts and preventing antibody mediated rejection.

[A case of hypophosphatasia in an infant]. / Su di un caso di ipofosfatasia nel lattante.

Hypophosphatasia is a rare familial disease characterized by abnormalities of the skeleton, low serum alkaline phosphatase level and presence of abnormal quantities of phosphorylethanolamine in plasma and urine. The biochemical bases of inadequate calcification of the bone matrix are unknown. We report the case of a 4 month-old infant who presented symptoms of rickets. Laboratory analysis showed normal serum Ca and P levels, low serum alkaline phosphatase activity, PEA level increased in plasma and urine. X-ray examination of the bones disclosed poor mineralization and the presence of focal defects of the skull. The therapeutic problems are discussed.