Effects of acute and chronic caffeine intake on intravenous self-administration of morphine in two rat strains.

Effects of acute and chronic caffeine intake on the level and pattern of morphine self-administration behavior in WAG/G and Fisher-344 rats were studied. Both acute and chronic caffeine intake decreased morphine self-administration only in WAG/G rats, which attested to increased sensitivity of these rats to reinforcing effects of morphine. Possible relationship between the observed changes and increased anxiety in rats receiving caffeine is discussed.

[Monoclonal antibodies to bovine prolactin interacting with human prolactin]. / Monoklonal'nye antitela k prolaktinu krupnogo rogatogo skota, vzaimodeistvuiushchie s prolaktinom cheloveka.

Two stable hybridomas producing antibodies (Mab 1 and Mab 2) to bovine prolactin and belonging to the IgG1 subclass have been prepared. The cross-reactivity of Mab 1 and Mab 2 with some structurally similar pituitary protein (human, pig, whale, rat prolactins, bovine and human somatotropins) using indirect immunoenzymatic assay, was studied. It has been shown that Mab 2 reacts specifically only with bovine prolactin whereas Mab 1 interacts with human prolactin and prolactins of different animals. The specificity of Mab 1 to human prolactin was confirmed by immunoradiodetection assay on nitrocellulose filters. The data obtained give evidence of the existence of at least two different sterically nonoverlapping epitopes: one of them is specific exclusively for bovine prolactin and the other one is common, i.e. extraspecific.

[Obtaining of monoclonal antibodies to human growth hormone and their characteristics]. / Poluchenie monoklonal'nykh antitel k gormonu rosta cheloveka i ikh kharakteristika.

The aim of present study was to obtain the hybridomas producing monoclonal antibodies against human growth hormone (Mabs hGH), to investigate the properties of the obtained Mabs and the possibility of their application in immunoanalytical systems. Two hybridomas secreting Mabs against hGH and belonging to the IgGI subclass have been obtained. The cross-reactivity of the Mabs with structurally similar to hGH hormones (hGHbio, hPL, hPRL, bGH, bPRL, pPRL) using indirect IFA has been studied. It has been shown that Mabs hGHI and Mabs hGH2 are directed to common specific antigenic determinant i.e. they have the same epitope specificity and don't react with other structurally related hormones, i.-e. this determinant is unique for hGH. The obtained Mabs hGH2 would find application for determination hGH by immunochemical methods in fractions while the hormone isolation from pituitaries and hGH obtained recombinant DNA methodology. The development of immunosorbents on the basis Mabs hGH2 seems to be perspective. Application of this immunosorbent may give possibility to optimize hormone isolation process.

[Monoclonal antibodies against the beta subunit of human chorionic gonadotropin]. / Monoklonal'nye antitela protiv beta-sub''edinitsy khorionicheskogo gonadotropina cheloveka.

The aim of investigation was creation of hybridomas, which produce monoclonal antibodies to the beta-subunit of human chorionic gonadotropin (beta-hCG), characterization of monoclonal antibodies, which necessary for hCG immunoassay in biological fluids, as an immunological methods of detection of early stage of pregnancy and choriocarcinoma. 4 hybridomas, producing monoclonal antibodies to-hCG of IgG1 isotype, were created. On the base of monoclonal antibodies, which produced by D2 hybridoma cell line, test-systems for RIA of hCG in blood serum and urine were elaborated. These test-systems can be used in medical practice for diagnosis of early stages of pregnancy and choriocarcinoma.

[Ultrastructure of the ovaries of guinea pigs immunized with preparations of purified and unpurified antigens of the zona pellucida of xenogenic ova]. / Ul'trastruktura iaichnikov morskikh svinok, immunizirovannykh preparatami ochishchennykh i neochishchennykh antigenov prozrachnoi zony ksenogennykh iaitsekletok.

The state of the guinea pig ovaries has been studied electron microscopically in 8-15 and 30 days after an active immunization course with purified and non-purified antigens of the ovum zona pellucida has been completed. Both antigens produce early total morphofunctional rearrangements in the ovaries: increasing process of follicular atresia and disturbance of folliculogenesis, accompanied with hyperplasia of the interstitial tissue. The non-purified antigen produces more pronounced changes in the cavitary follicles: disturbances of cellular communications, barrier function of the follicular epithelium becomes weak, its respiratory, plastic and secretory function changes with subsequent distension of the follicle and with an enhanced development of connective tissue in its membranes. Injection of the purified antigen results in progressive atresia of the cavitary follicles and in hyperplasia of the interstitial tissue. Reduction of the corpus luteum is not observed.

[Cross-reacting antigens of the mammalian ovular zona pellucida]. / Perekrestno-reagiruiushchie antigeny blestiashchei obolochki iaitsekletok mlekopitaiushchikh.

Antigens of zona pellucida (ZP) of different mammalian species, including the man, were studied by means of various immunochemical methods. The analysis was carried out using rabbit antisera to the pig, mouse, guenon and Java macaque eggs. After immunoadsorption (by blood serum and tissue extracts) these anti-ZP-sera reacted with water-insoluble ZP components only (in immunofluorescence). The adsorbed anti-ZP-sera were specific not only to homologous ZP, but also to ZP of other mammalian species. The spectrum of antigens of the ape ZP was most closely related to that of human ZP. The pig eggs contained antigens common with the human ZP as well. The antiserum to the mouse ZP did not react with ZP of man, ape, pig, cow, rabbit and other species under study. The blood of sterile women contained the antibodies reacting (in immunofluorescence) with ZP of mouse, pig, guinea pig, sheep and man (very weakly). The data obtained suggest the complexity of antigenic mosaic of the mammalian ZP including both "cross-reacting" (common) antigens and species specific ones.